Browsing by Author "Yavaş, Mehmet Cihan"

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The effect of radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase (PON1)
(Annals of Medical Research, 2023) Yavaş, Mehmet Cihan; Kilitçi, Asuman
Aim: The development of technology increases the rate of everyone having a smartphone. Therefore, the possible biological effects of these devices are of concern. In this study, we aimed to investigate the effect of 2100 MHz radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase 1 level. Materials and Methods: Within the scope of the study, a 2100 MHz radiofrequency electromagnetic radiation model was created. In our study, we used Sprague Dawley male rats. Two groups were made as sham-control and exposure group (5 h a day for 2 weeks). Liver tissue and serum paraoxonase were studied. Results: The paraoxonase 1 value of the exposure group was higher than the sham control group, and did not have statistically important difference in the comparison of exposure and sham control groups (p>0.05). Did not have important difference in histopathological parameters of rat liver tissue (p>0.05). Conclusion: Although it seems that radiofrequency radiation does not cause liver dam age, more detailed studies with short- and long-term exposure are needed
The Effect of Radiofrequency Electromagnetic Radiation on Rat Liver Tissue and Serum Paraoxonase (PON1)
(2023) Kilitci, Asuman; Yavaş, Mehmet Cihan
Aim: The development of technology increases the rate of everyone having a smartphone. Therefore, the possible biological effects of these devices are of concern. In this study, we aimed to investigate the effect of 2100 MHz radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase 1 level. Materials and Methods: Within the scope of the study, a 2100 MHz radiofrequency electromagnetic radiation model was created. In our study, we used Sprague Dawley male rats. Two groups were made as sham-control and exposure group (5 h a day for 2 weeks). Liver tissue and serum paraoxonase were studied. Results: The paraoxonase 1 value of the exposure group was higher than the sham control group, and did not have statistically important difference in the comparison of exposure and sham control groups (p>0.05). Did not have important difference in histopathological parameters of rat liver tissue (p>0.05). Conclusion: Although it seems that radiofrequency radiation does not cause liver damage, more detailed studies with short- and long-term exposure are needed.
Effect of Tartrazine and Curcumin Intake on Serum Carcinoembryonic Antigen (CEA) and Stomach Histopathology in Rats
(2025) Yavaş, Mehmet Cihan; Toktaş, İzzettin; Hakseven, Musluh; Varlı, Metin; Basmaci, Guldidar
Objective: Food colorants are known to induce physiological and biochemical changes in tissues, raising concerns about their potential health effects. The purpose of this study was to assess tartrazine's effects and curcumin supplementation on stomach tissue and serum carcinoembryonic antigen (CEA) levels in rats. Methods: Thirty-five male rats were randomly divided into five groups (n=7 per group) and treated via oral gavage for three weeks: Group 1 consisted of a control group, Group 2 of low-dose tartrazine, Group 3 of high-dose tartrazine, Group 4 of low-dose tartrazine + curcumin, and Group 5 of high-dose tartrazine + curcumin. Serum CEA levels were measured and stomach tissues were subjected to histopathological examination. Results: Following tartrazine treatment, serum CEA levels were higher than in the control group; however, this difference was not of statistical significance (p > 0.05). CEA levels were somewhat lowered by co-administration of curcumin. When compared to the control, histopathological investigation showed that all tartrazine-treated groups had degenerated stomach tissues. Hyperkeratosis was notably increased in all experimental groups relative to the control group, with the most pronounced effect observed in Group 5 (p < 0.001). While hyperplasia in Group 2 did not differ significantly from the control (p > 0.05), Groups 3, 4, and 5 exhibited a statistically notable rise (p < 0.05). Conclusion: These results show that tartrazine may induce dose-dependent histopathological alterations in stomach tissue, and curcumin may exert a modest protective effect.
Investigation of Genotoxic Effects of Diethyl Phthalate (DEP) Exposure on Thyroid Function in Wistar Albino Female Rats
(2025) Kömürcü, Ayşe; Yavaş, Mehmet Cihan
Yaygın olarak kullanılan bir plastikleştirici olan dietil ftalat (DEP), endokrin bozulma ve oksidatif stres ile ilişkilendirilmektedir. Bu çalışmanın amacı, subakut DEP maruziyetinin Wistar dişi sıçanların tiroit dokusu üzerindeki genotoksik etkilerini; tiroit hormon düzeyleri, oksidatif stres parametreleri, DNA hasarı ve histomorfolojik değişiklikler üzerinden değerlendirildi. Yirmi sekiz dişi Wistar albino dişi sıçanı dört gruba (n=7) ayrılmıştır: kontrol, 100 mg/kg/gün, 300 mg/kg/gün ve 600 mg/kg/gün DEP uygulama grupları. DEP, oral gavaj yoluyla 21 gün süreyle verildi. Deney sonunda serum TSH, T3 ve T4 düzeyleri ELISA ile; oksidatif stres belirteçleri (TAS, TOS, OSI) biyokimyasal analizlerle; DNA hasarı ise Comet Assay yöntemi ile değerlendirildi. Histopatolojik incelemeler yapılmış ve vücut ağırlıkları takip edilmiştir. Kontrol grubu ile karşılaştırmalarda; T3, T4 düzeylerinde azalma (P<0.05), TSH düzeyinde ise artış izlenmiştir (p<0.05), TAS ve OSI düzeyinde azalma (p<0.05), TOS düzeyinde ise artış izlendi (p<0.05). Comet Assay sonuçları, DNA bütünlüğünde bozulma ve hücresel hasarın DEP dozunun artması orantılı yükseldiği (kuyrukta % DNA (tail DNA)) görüldü (p<0.05). Bununla beraber tiroit dokusunda belirgin bir histopatolojik değişime rastlandı. Elde edilen bulgular, DEP'nin tiroit fonksiyonu üzerinde doza bağlı genotoksik etkilere sahip olabileceğini, endokrin ve hücresel düzeyde ciddi bozukluklara yol açabileceğini gösterdi. Bu sonuçlar, DEP'ye karşı çevresel ve bireysel maruziyetin sınırlandırılması gerekliliğini ortaya koydu.
Tartrazine-Induced Nephrotoxicity via Oxidative and Genotoxic Pathways in Rats: Regulatory Insights and the Nephroprotective Role of Curcumin
(Academic Press Inc Elsevier Science, 2026) Demirtaş, Selim; Tan, Fazile Canturk; Keskin, Cumali; Varol, Salih; Yavaş, Mehmet Cihan; Cantürk Tan, Fazile
This study evaluated tartrazine-induced nephrotoxicity and the protective effects of curcumin in rats. Thirty-five male Wistar albino rats were assigned to five groups (n = 7): control; tartrazine 10 mg/kg/day; tartrazine 100 mg/kg/day; tartrazine 10 mg/kg/day + curcumin 20 mg/kg/day; and tartrazine 100 mg/kg/day + curcumin 20 mg/kg/day. After 21 days, blood and kidney samples were analyzed for biochemical, oxidative, genotoxic, and histopathological changes. High-dose tartrazine significantly elevated serum urea and creatinine levels compared with controls (urea, p = 0.033; creatinine, p < 0.001), indicating renal dysfunction. Curcumin co-treatment mitigated these elevations. Total antioxidant status (TAS) was elevated by tartrazine exposure but decreased with curcumin supplementation (p < 0.001), total oxidant status (TOS) showed a non-significant increasing trend and was reduced by curcumin. Compared to the control group, MDA levels decreased with low-dose tartrazine and increased with high-dose tartrazine, while curcumin supplementation increased levels (p < 0.05). Comet assay and histopathological analyses confirmed dose-dependent DNA and tissue damage, both of which were alleviated by curcumin. Overall, short-term tartrazine exposure may induce renal biochemical, oxidative, and genotoxic alterations in rats under experimental conditions, particularly at doses exceeding the ADI level. The antioxidant properties of curcumin may mitigate the negative effects of food dyes.