Temel Tıp Bilimleri Bölümü

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Response of Zinc Levels During Aerobic Exercise: A Special Connection between Increased Metabolic Stress and Altered Oxidant to Antioxidant Balance
(Anatolia Sport Research, 2022) Kaya,Hüsamettin; Uğraş, Seda; Özdenk, Çağrı
ABSTRACT Aim: It was aimed to evaluate aerobic exercise induced metabolic stress on plasma zinc levels and its connection with oxidant antioxidant status. Material and Method: Thirty sedentary male performed a 45 minutes of running exercise at the work load correspond to lactate threshold reflecting moderate aerobic intensity. Blood samples were taken before and after exercise. Plasma zinc, malondialdehyde (MDA), total oxidant status (TOS) and total antioxidant status (TAS) were measured using HPLC and ELISA kits. Results: Exercise resulted significant decreases in zinc levels which is closely associated with increased TOS and MDA levels. Conclusion: Zinc shows great influence on aerobic exercise induced increased metabolic stress as indicated with significant alteration in TOS, TAS and MDA levels. Thus zinc supplement should be considered during long period high intensity exercise to support muscle damaged. Keywords: Exercise, Zinc, Oxidative stress, TAS, TOS ÖZET Amaç: Aerobik egzersize bağlı metabolik stresin plazma çinko düzeyleri ve oksidan antioksidan durumu ile ilişkisinin değerlendirilmesi amaçlandı. Gereç ve Yöntem: Otuz sedanter erkek, orta derecede aerobik yoğunluğu yansıtan laktat eşiğine karşılık gelen iş yükünde 45 dakikalık koşu egzersizi yaptı. Egzersiz öncesi ve sonrası kan örnekleri alındı. Plazma çinko, malondialdehit (MDA), toplam oksidan durumu (TOS) ve toplam antioksidan durumu (TAS), HPLC ve ELISA kitleri kullanılarak ölçüldü. Bulgular: Egzersiz, artan TOS ve MDA seviyeleri ile yakından ilişkili olan çinko seviyelerinde önemli düşüşlere neden oldu. Sonuç: Çinko, TOS, TAS ve MDA düzeylerinde önemli değişiklik ile belirtildiği gibi, aerobik egzersizin neden olduğu artan metabolik stres üzerinde büyük bir etki göstermektedir. Bu nedenle, uzun süreli yüksek yoğunluklu egzersiz sırasında hasarlı kasları desteklemek için çinko takviyesi düşünülmelidir.
Citation - WoS: 2
Citation - Scopus: 2
Thiol–disulfide balance and trace element levels in patients with seasonal allergic rhinitis
(African Health Sciences, 2022) Savas, Hasan Basri; Gunizi, Huseyin
Abstract Background: The prevalence of allergic diseases is gradually increasing worldwide. The most common such allergic disease is allergic rhinitis (AR). Objective: The present study investigated the possible relationship between seasonal AR and the thiol-disulfide balance and zinc and copper levels in adult individuals. Study Design and Methods: 130 male and female adults were included in the study. The participants’ serum thiol-disulfide balance and zinc and copper levels were measured spectrophotometrically using commercial kits. Statistical significance was accepted as p < 0.05 between the groups. Results: The serum copper (p = 0.001), native thiol (p = 0.006), reduced thiol (p < 0.001), and thiol oxidation reduction ratio (p < 0.001) levels were significantly lower in the seasonal AR group than in the control group. Conclusion: In AR patients, the low level of copper, which is an important trace element, the deterioration of the thiol-disulfide balance, which represents a unique indicator of the oxidant-antioxidant balance, the increased disulfide level caused by oxidative stress, and the decreased native thiol level can all serve as important biochemical markers.
Beslenme Biyokimyası
(Nobel Akademik Yayıncılık, 2022) Savaş, Hasan Basri
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COVID-19 Pandemisi Süresince Huzurevinde Kalan Yaşlıların Hijyen Alışkanlıkları Üzerine Bir İnceleme
(Muş Alparslan Üniversitesi, 2023) Dicle, Yalçın; Dicle, Yalçın; Pamuk, Deniz; Yalçın
Abstract: Objective: Because the number of aging population is rising gradually this descriptive study is to evaluate the behaviors of individuals’ hygiene residing in residential homes during COVID-19 Pandemic. Method: The study was conducted through face-to-face interviews with 100 elderly individuals residing in nursing homes in two cities within the borders of the Eastern Anatolia Region, one of which has normal and the other metropolitan status. Data were collected with a socio-demographic information questionnaire and a questionnaire examining behaviors related to the mask-distance-cleanliness triad to protect against disease. Pearson chi-square analysis was applied to express the relationship between the groups. Results: A statistically significant relationship was found between the variable of the participants' COVID-19 recovery status and the use of masks indoors. (p: 0,008; r= .514) It was determined that there was a statistically significant relationship between the variable of COVID-19 transmission status of residential home residents and the use of hand sanitizer also there was a statistically significant relationship between the variable of the participants' status of having COVID-19 and the frequency of visitors to their rooms (p: 0,001; r= .646). Conclusions: Although the hygiene behaviors gained in order to prevent and reduce transmission during the pandemic are approaching the ideal level, it has been understood that more attention should be paid to the use of masks and social distance.
Anti-angiogenic and oxidative effects of brilliant blue at different concentrations in chorioallantoic membrane model
(Modestum, 2023) Savaş, Hasan Basri
Introduction: Artificial food colorings are increasingly used to make the color and appearance of foods more attractive. One commonly used food dye is E133, international-coded brilliant blue (BB). According to the European Food Safety Authority panel results in 2010, it was determined that BB daily remained below the acceptable daily intake (ADI) value for adults and exceeded ADI value for children. The purpose of this study was to investigate the effects of BB on angiogenesis and oxidative stress in the chorioallantoic membrane model. Materials & methods: In this investigation, fertilized chick eggs free of specific pathogens were used. The eggs that were not fully grown or fertilized were excluded. 50 embryos were distributed into five groups of 10 each. The negative control group was the control group, the positive control group was the bevacizumab group, and three different BB dosages (10-4 M, 10-5 M, and 10-6 M) were identified. At the end of the experiment, anti-angiogenesis scoring, and total antioxidant-oxidant capacity were evaluated. Results: According to the average score values, the control group had no anti-angiogenic impact, but the bevacizumab group had a strong anti-angiogenic effect (average score 1.1). Furthermore, the 10-4 M BB group had a weak anti-angiogenic impact (average score of 0.7), while the 10-5 M and 10-6 M BB groups had no anti-angiogenic effect (average score of 0.4 and 0.2, respectively). As a result of one-way analysis of variance test, it was seen that BB significantly increased total oxidant capacity and oxidative stress index values in proportion to the increase in dose (p<0.05). Conclusions: BB’s oxidant and anti-angiogenic effects indicate that high doses of processed foods containing artificial food dyes carry a risk for viable growth. Since there are not enough studies in the literature showing the oxidant or antiangiogenic effects of BB in chorioallantoic membrane model, the original data we presented in this study are pioneering.
Citation - WoS: 1
Citation - Scopus: 1
The distribution and the antimicrobial susceptibility features of microorganisms isolated from the burn wounds; a ten-year retrospective analysis
(Oxford University Press, 2023) Dicle, Yalçın; Dicle, Yalçın; Bayram, Yasemin; Parlak, Mehmet; Akış, Feride; Çallı, İskan
In this study we aimed to evaluate the distribution features and antimicrobial susceptibility test results of the microorganisms isolated from the wounds of pediatric and adult burn patients. The culture and susceptibility test results of the microorganisms isolated from the wound swabs of the patients hospitalised in a tertiary burn care center in 10-years period, were retrospectively screened on the microbiology department databases. Their distribution of isolated microorganisms regarding species and susceptibility test results were compared with previous studies. A total of 367 microorganisms isolated from the burn wounds of 293 patients (13± 18.9 years, F/M: 0.93) (73 adult and 220 pediatric patients), were included in this study. A solitary agent was isolated in 239 (81.6%) patients, while two were isolated in 43 (14.7%) and three or more agents in 11 (3.8%). From these, 33% of the isolated microorganisms were gram positive cocci, 61% were gram negative bacteria and 6% were Candida spp. The most common isolated microorganisms were Staphylococcus aureus (18.5%), Pseudomonas spp. (16.9) and Escherichia coli (%11.2), while the least common was Streptococcus spp. (2.5%). Methicillin resistance was 15% among the S. aureus strains. No resistance was observed against levofloxacin, vancomycin, teicoplanin, linezolid, daptomycin, fusidic acid and tigecycline in S. aureus strains. The highest resistance rates were observed against levofloxacin (64%), tobramycin (64%), pip/tazobactam (63%), imipenem (63%), and the lowest against colistin (5%) and ceftazidime (29%), among Pseudomonas spp. The most common causative agents in burn wound infections and their current antimicrobial susceptibility features should be well identified, in order for prevention of serious complications and optimal management the condition to occur.
The protective role of selenium in an experimental high fructose corn syrup exposure.
(Modestum, 2023) Savaş, Hasan Basri
Objective: Nowadays, fructose is recognized as a significant health threat. Prepared foods containing fructose are consumed more because they do not create a feeling of satiety. Selenium is an essential trace element with antioxidant and cell protective properties. In this study, the effect of high fructose corn syrup, which is used as a sweetener in many foods and beverages and consumed during pregnancy, and the possible protective role of selenium in this effect were investigated and examined. Methods: Fertilized specific pathogen-free eggs were used in our study. These eggs were divided equally into four groups. Each group was allocated 10 eggs containing viable embryos. These groups are pre-process control, post-process control, high fructose corn syrup (HFCS55), and high fructose corn syrup and selenium (HFCS-55+Se 10-6 ) groups. Pellets containing and containing no active substance (HFCS-55, HFCS-55+Se 10-6 ) were carefully placed on the chorioallantoic membrane (CAM) of each egg in these groups. Oxidative stress status in all groups was determined by total oxidative stress (TOS) and total antioxidant capacity (TAC) methods. Results: In our study, a significant increase in TOS levels and a significant decrease in TAC levels were observed in egg groups given HFCS compared to other groups (p<0.05). The OSI value was shown to be lower in the group given HFCS+Se. Conclusion: As a result, HFCS was shown to increase oxidative stress. In line with our data, it has been shown that Se, plays a protective role against oxidative stress.
Citation - WoS: 1
Citation - Scopus: 1
Evaluation of the pleth variability index, perfusion index, and other physiological parameters after COVID-19
(Verduci Editore srl, 2023) Gökdemir, Gül Şahika
Objective: The aim of this study was to observe the changes in pleth variability index (PVI), perfusion index (PI) and other hemodynamic parameters in adult individuals who had had Coronavirus disease 2019 (COVID-19) and were currently living a normal life. A further aim was to draw attention to the fact that some hemodynamic changes after COVID-19 may cause long-term health problems. Patients and methods: A total of 174 adult individuals who had had COVID-19 and were currently living a normal life and 56 healthy individuals with similar demographic characteristics who had not had COVID-19 were included in the study. The PI, PVI, oxygen saturation (SpO2), pulse rate (PR), total hemoglobin (Hgb), oxygen reserve index (ORI), and blood pressure values were measured by Masimo Radical 7. The data of individuals who had and did not have COVID-19 before were compared. Results: The mean PVI (p = 0.008) and PI (p < 0.001) were significantly lower in people who had been exposed to COVID-19. Likewise, the mean of ORI, SpO2, and SpOC values was observed to be significantly lower in participants exposed to COVID-19 disease (p < 0.001, p < 0.001, and p = 0.006, respectively). The PVI had a positive correlation with body mass index (BMI) (r = 0.263, p < 0.001) and a negative correlation with SpO2 (r = -0.194, p = 0.003) and PR (r = 0.190, p = 0.004). Conclusions: The PVI, PI, and other physiological parameters could potentially be useful for monitoring COVID-19 patients and evaluating their response to therapy. We believe that people who have been exposed to COVID-19 may be more susceptible to other diseases; therefore, they should be subjected to regular clinical checks.
Retrospective Evaluation of Tularemia Cases in Muş Province: 2011-2022
(Mardin Artuklu University, 2023) Dicle, Yalçın; Aydın, Elif; Şen Gündoğan, Nesibe Efruz; Yalçın
Objective: Tularemia is a bacterial disease of humans, wild and domestic animals. Recently, an increase in human tularemia cases has been observed in many countries worldwide. This study aimed to calculate the incidence of tularemia cases in Muş, determine the seasonal distribution of cases, and describe the socio demographic and clinical characteristics of tularemia cases detected over a twelve-year period. Methods: Data from 162 patients diagnosed clinically and serologically with tularemia and admitted to health facilities in Muş province between 2011 and 2022 were retrospectively analysed. Results: In our study, among 162 tularemia patients with F. tularensis microagglutination test titres of 1/160 and above, the ratio of male and female cases was equal (50%), and the mean age was 24.51 years. In the twelve-year evaluation, there were two major epidemics, and the most cases were seen in 2018 with 37 cases. Most cases were observed in winter (38.3%) and autumn (25.3%). Oropharyngeal tularemia was the most common form (64.2%) among the cases evaluated. Similar disease was found in 33.3% of patients living in the same neighbourhood as the patients, and no mortality was observed in any of the patients. Conclusion: As the oropharyngeal form is the most common, contact with contaminated water should be questioned, rodent and tick populations should be monitored and detailed epidemiological studies should be conducted. We believe that hygiene and sanitation measures are important with regard to tularemia outbreaks.
Anti-Angiogenic and Oxidant Effects of Monosodium Glutamate at Different Concentrations in Chorioallantoic Membrane Model
(Muğla Sıtkı Koçman Üniversitesi, 2023) Savaş, Hasan Basri
Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.
Is There a Relationship Between First-Trimester Aneuploidy Screening Serum Biomarker Values and Nuchal Translucency Measurements and the Development of Retinopathy of Prematurity (ROP) in Premature Infants?
(Nature, 2023) Savaş, Hasan Basri
Abstract Purpose The purpose of this study is the evaluation of serum biomarker and nuchal translucency (NT) values measured during first-trimester aneuploidy screening in terms of the development of retinopathy of prematurity (ROP) in premature infants and investigation of whether the development of ROP is associated with these parameters. Methods In this retrospective cohort study, 3,750 premature infants who underwent ROP screening from 2016 to 2021 were identified from the hospital medical record system. Among 2,130 premature babies screened for firsttrimester aneuploidy, 166 babies whose mothers had single pregnancies were screened by the same method and showed the same clinical course in both eyes were included in the study. The infants were divided into two groups according to the presence of ROP, and those with ROP were further evaluated in two groups according to the presence of proliferation. The groups were compared in terms of the serum values of human chorionic gonadotropin and pregnancy-associated plasma protein A, among aneuploidy screening biomarkers, and NT measurements. Results There was no significant difference in the evaluated serum biomarker values and NT measurements between the ROP and non-ROP groups or between the proliferative ROP, non-proliferative ROP, and non-ROP groups. Conclusion Our results showed that first-trimester aneuploidy screening serum biomarker values and NT measurements were not associated with the development of ROP in premature infants.
Detection of Human Brucellosis by Brucellacapt and Rose Bengal Test in the Endemic Area
(Fazile Nur Ekinci Akdemir, 2023) Dicle, Yalçın; Dicle, Yalçın; Kaçtaş, Şerif; Gümüş, Ali Furkan
Abstract: Although brucellosis is the most common zoonosis in the world, it remains an infectious disease that has not received sufficient attention. There are a few tests detecting brucellosis by serum. Rose Bengal Test is an advantageous one. Our aim with this study was to compare Rose Bengal and Brucellacapt tests in serum samples and draw attention to the advantages of the test. Between June 2019 and June 2021, 7827 serum samples sent to the public health laboratory with a provisional diagnosis of brucellosis were evaluated. The Rose Bengal and Brucellacapt test (Vircell, Spain) was used to diagnose infection. Samples with antibody titer ≥1/160 were considered positive. Of the 7827 samples included in the study, 354 (4.6%) of the total 7677 serum samples tested were RBT positive, 118 (3.1%) of the 3776 samples tested were BCAP positive, and 118 (3.3%) of the 3626 samples tested were both RBT and BCAP positive. It was found that seropositivity was significantly higher in female patients (p<0.10). RBT and BCAP test positivity were most frequently observed in the 25-34 year age group. Considering brucellosis cases in terms of seasonal changes; 10.7% of cases were found in spring, 52.4% in summer, 20.9% in fall, and 16% in winter. In suspected cases, RBT is still considered the ideal detection method because it is easy to use, inexpensive, sensitive, and provides rapid results. It was concluded that the BCAP test is suitable for diagnosis due to its ability to detect blocker and high titer antibodies.
Anti-angiogenic and oxidative effects of sodium benzoate at different concentrations in chorioallantoic membrane model.
(Modestum, 2023) Savaş, Hasan Basri
Aim: Due to the increased consumption of packaged foods, exposure to food additives is also increasing. Sodium benzoate (SB), a frequently used food additive, is generally used in alcoholic beverages, fruit, and vegetable juices, carbonated soft drinks, canned food, and various sauces. It is used to inhibit the formation of mold, yeast, and bacteria. This study was carried out to investigate the effects of SB exposure on angiogenesis and oxidant-antioxidant balance. Materials & methods: Three different concentrations of SB, bevacizumab, and empty pellets were prepared, placed on chorioallantoic membrane (CAM), and examined for antiangiogenesis. Total antioxidant capacity (TAC) and total oxidant capacity (TOC) measurements were made in the albumen samples, and oxidative stress index (OSI) value was calculated. Results: The control group had no anti-angiogenic effect, but the bevacizumab group had a strong anti-angiogenic effect.10-3 M SB had a weak anti-angiogenic effect, but 10-4 M SB and 10-5 M SB showed no anti-angiogenic effect. TOC levels increased with SB in a dose-dependent manner. TAC levels decreased depending on the dose in the experimental groups with SB application. OSI levels increased depending on the dose increase in SB. Conclusions: SB exposure caused a dose-dependent increase in oxidative stress and antiangiogenic effect in CAM model.
The Oxidative and Anti-Angiogenic Effects of Acrylamide in Chorioallantoic Membrane Model
(Akdeniz Üniversitesi, 2023) Savaş, Hasan Basri
Monosodium glutamate (MSG) is a flavor-enhancing food additive. MSG exposure is rising day by day because of the high commercial food consumption. MSG exposure causes damage to various tissues and organs. The aim of this study is to investigate the effects of MSG on angiogenesis and oxidant-antioxidant balance. Three different concentrations of MSG (10-4 M, 10-5 M, and 10-6 M), control, and the bevacizumab (10-6 M) were prepared and placed on the chorioallantoic membrane (CAM) of the embryos. Albumen was taken from the embryos before and after the experiment. Angiogenesis was investigated through the window that was opened on the eggshell. Angiogenesis was found to be normal in the control and 10-6 M MSG group (average score: 0.3). Anti-angiogenic effects were moderate in the 10-5 M MSG group (average score: 0.5) and in the 10-4 M MSG group (average score: 0.7), and strong in the bevacizumab group (average score: 1.1). According to our results, MSG shows anti-angiogenic properties in higher doses. MSG increased oxidative stress. According to the results of our research, it is seen that MSG inhibits angiogenesis in a dose-dependent manner in the CAM model and may cause an increase in oxidative damage by disrupting the oxidant-antioxidant balance. Since no previous study has been found in the literature regarding the effects of MSG on angiogenesis and oxidant-antioxidant balance in the CAM model, we think our results will fill an important gap in the literature.
Case report: physical therapy and rehabilitation process of a patient diagnosed with achondroplasia
(Journal of Medical Topics and Updates, 2023) Canbay Durmaz, Sevda; Güven, Ümran; Canbay, Ali; Özbağ, Davut; solgun Dağ, Selma
Achondroplasia is an autosomal dominant disorder. But 80% of cases are sporadic. Its incidence is between 1/25000 and 1.5/10000. Significant shortening of the femur and humerus occurs in patients with achondroplasia. This shortness causes the appearance of rhizomelic type of dwarfism. Since the skull of patients with achondroplasia is large during delivery, it may cause difficult delivery. During infancy, hypotonia is more dominant. Many patients also have delayed motor development. Our aim in the study is to present the successful physical therapy and rehabilitation process of our patient diagnosed with achondroplasia for one year and her condition from diagnosis to treatment. Our patient was born via cesarean section on 12.02.2020. The weight of the baby girl was 3500 grams. The mother's height was 162 cm. and the father's height was 174 cm. The mother was 23 years old and the father 27 years old. The same diagnosis was not present in any living relative of the patient. The patient was 1 year old when she applied to our center. She had completed head control and had not yet acquired the skills of rolling over, crawling, and sitting without support. Gross motor skills such as assisted standing and stepping were not developed. In this direction, after a 1-year physical therapy and rehabilitation period applied in 2 sessions a week, the patient gained the ability to walk independently.
70 Adımda Sağlıklı Beslenme
(S.S. International Publishing, 2023) Savaş, Hasan Basri
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Clınıcal Sıgnıfıcance of HDL-C Values And Routıne Parameters Of Inflammatıon Such As Hs-Crp, Lenfocyte And Neutrofıl Ratıos In Covıd-19 Patıents
(İZMİR ATATÜRK EĞİTİM HASTANESİ TIP DERGİSİ, 2023) Gökdemir, Gül Şahika; Nas, Cemal
Objective: The study aimed to evaluate the prognostic significance of high-density lipoprotein cholesterol (HDL-C) levels, alongside markers including the neutrophil/HDL-C ratio (NHR) and lymphocyte/HDL-C ratio (LHR), on the mortality of COVID-19 patients. It also examined the potential clinical application of these biomarkers in the management of future healthcare crises, despite the end of the COVID-19 pandemic. Materials and Methods: Patients diagnosed with COVID-19 were retrospectively analysed between March 2020 and August 2022. A total of 367 patients were categorised into two groups: survivors (group 2) and non-survivors (group 1). Patient demographics, biochemical and haematological parameters obtained from blood samples, high-sensitivity C-reactive protein (hs-CRP), neutrophil-to-lymphocyte ratio (NLR), monocyte/HDL-C ratio (MHR), NHR, LHR, platelet/HDL-C ratio (PHR) were calculated. Patient data were analysed using SPSS 26 and statistical differences of P<0.05 were considered significant. Results: Among the patients analyzed in the study, the living group constituted 89.9% of individuals, while the deceased group represented 10.1%. The neutrophil levels were significantly higher in the deceased group, and NLR and NHR values were statistically significant (P<0.001 and P=0.001, respectively). There was no significant difference in HDL-C levels. Moreover, no significant differences in LHR, MHR, and PHR levels were observed between groups. The hs-CRP levels were significantly higher in the deceased (P<0.001). Correlation analysis indicated a negative correlation between HDL-C and LHR and NHR, and significant correlations were found for other markers. ROC analysis showed that LHR and NHR were significant in discriminating deceased patients. Conclusion: Biochemical and haematological parameters, especially markers such as NLR and NHR, can potentially be used to assess the risk of death in COVID-19 patients. These markers may be valuable in predicting the prognosis of patients and could be used in future similar health crises.
The Effect of Gliclazide use on BDNF and NGF Levels in Rats with Diabetes Mellitus
(2023) Gökdemir, Gül Şahika; Baylan,Mukadder
Objective: In this study, the effects of gliclazides, a second generation sulfonylurea group, on BDNF and NGF plasma levels, which are considered neurodegeneration biomarkers, will be examined. When designing our study, we assumed that gliazides might have positive neuronal effects. Thus, the possible positive effects of gliclazide will be emphasized in our study. Methods: In the experiment, 21 adult male Wistar-Albino rats were used. Serum BDNF and NGF levels were determined by analyzing with enzyme-linked immunosorbent assay kit in accordance with the recommendations. Results: BDNF levels were significantly lower in gliclazide-treated diabetic rats and nonmedicated diabetic rats compared to the healthy control group (p=0.017, p<0.001, respectively). Although the BDNF level of rats with diabetes given gliclazide was increased compared to rats with and without diabetes, this difference was not significant (p=0.107). Similarly, NGF levels were significantly lower in rats given gliclazide (p=0.009) and diabetic rats not given gliclazide (p=0.001) compared to the healthy control group. When the diabetic groups were compared among themselves, although the NGF level was increased in the gliclazide group, this difference was not statistically significant (p=0.638). The differences between the groups were significant in cyclic AMP regulatory element binding (p<0.001), c-FOS (p<0.001), amyloid precursor protein (p<0.001), B-SECRETASE1 (p=0.004), and doublecortin (p<0.001) levels. Conclusion: As a result, serum BDNF and NGF levels were significantly higher in non-diabetic healthy control group rats than in diabetic rats. While low serum levels of BDNF and NGF neurotrophins, which increase in neurodegeneration, were observed in diabetic rats, this level was observed to be higher in diabetic rats given gliclazide.
The effect of radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase (PON1)
(Annals of Medical Research, 2023) Yavaş, Mehmet Cihan; Kilitçi, Asuman
Aim: The development of technology increases the rate of everyone having a smartphone. Therefore, the possible biological effects of these devices are of concern. In this study, we aimed to investigate the effect of 2100 MHz radiofrequency electromagnetic radiation on rat liver tissue and serum paraoxonase 1 level. Materials and Methods: Within the scope of the study, a 2100 MHz radiofrequency electromagnetic radiation model was created. In our study, we used Sprague Dawley male rats. Two groups were made as sham-control and exposure group (5 h a day for 2 weeks). Liver tissue and serum paraoxonase were studied. Results: The paraoxonase 1 value of the exposure group was higher than the sham control group, and did not have statistically important difference in the comparison of exposure and sham control groups (p>0.05). Did not have important difference in histopathological parameters of rat liver tissue (p>0.05). Conclusion: Although it seems that radiofrequency radiation does not cause liver dam age, more detailed studies with short- and long-term exposure are needed
A Study on The Presence of Legionella pneumophila in Hospital Water Samples from Eastern Turkey
(Dicle Üniversitesi Tıp Fakültesi, 2023) Dicle, Yalçın; Dicle, Yalçın; Tuna, Duygu Kübra
Objective: Legionnaires' disease is a fatal form of pneumonia brought on by an infection mostly caused by the 60 different species of Legionella pneumophila. Legionnaires' illness is caused by Legionella spp.-contaminated water systems. In this work, we sought to analyze Legionella species, serogroups (SG), and contamination in the water sources of hospitals in five regions in eastern Turkey. Methods: Between January 2017 and December 2018, a total of 1008 samples were examined, including 2 cooling towers, 62 hot water tanks, 104 cold water tanks, and 840 faucet shower heads. Samples were collected by the standard culture method L. pneumophila SG 1, it was analyzed for L. pneumophila SG 2-16 and Legionella spp. The samples were inoculated into BCYE and GVPC medium, and the colonies were assessed using a latex agglutination test, followed by species- and serotype-level identifications. Results: In our study, a total of 1008 water samples were examined, of which 35.31% (356) belonged to 2017, while 64.68% (652) belonged to 2018. 83.33% of the water samples were taken from faucets and shower heads, 10.32% from the cold water tank, 6.15% from the hot water tank, and 0.2% from the cooling tower, and the highest positivity rate was observed in the hot water tank with 12.60%. 7.04% (71) of the samples were positive, and 16.9% (12) of the positive samples were L. pneumophila SG 1, 77.46% (55) L. pneumophila SG 2-14 was detected, while 5.63% (4) were nonpneumophila (Legionella spp) it has been determined as. Conclusions: Legionella disease remains a significant public health threat. The water tanks of hospitals and hotels should be investigated more thoroughly, the necessary disinfection procedures should be carried out frequently. All hospitals should have water management policies, and towns and large buildings should establish comprehensive water system management programs that decrease Legionella growth and transmission. To enhance prevention measures and clinical diagnosis, we also need quicker ways of detecting Legionella in water systems and clinical samples.

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