PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

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Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Awareness of risk factors and warning signs of stroke among caregivers of patient with and not with stroke: Results from questionnaire
(Journal of Pakistan Medical Association, 2019) Usta Yeşilbalkan, Öznur; Karadakovan, Ayfer; Vural Doğru, Birgül; Akman, Perihan; Özel, Ebru; Bozkurt, Yücel
Objective: To assess the awareness/knowledge of stroke risk factors and warning signs among caregivers of patients with or without stroke. Methods: The cross-sectional, descriptive and comparative study was conducted in the neurology clinic and polyclinic of a university hospital in Izmir, Turkey, from March to July 2014, and comprised primary caregivers of patients with stroke in group 1 and those of patients with no stroke in group 2. The subjects were screened and data was collected using the Participant Information Form and the Questionnaire Form About Stroke. Warning signs and symptoms of stroke were compared between the two sets of caregivers. SPSS 17was used for data analysis. Results: Of the 203 respondents, 105(52%) were in group 1 and 98(48%) in group 2. Group 1 had better awareness than group 2 (p<0.05). In group 1, weakness was the most commonly recognised warning sign 101(96.2%), whereas dyspnoea 41(39%), was the least commonly identified. There was no relationship of stroke knowledge with educational level and age (p>0.05 each). Conclusions: Caregivers had a moderate knowledge of some of the warning signs and risk factors about stroke.
Comparison of the Breastfeeding Practices of Refugee Syrian Mothers and Native Turkish Mothers
(Breastfeeding Medicine, 2020) Değer Bayram, Vasfiye; Ertem, Melikşah; Çifçi, Sema
Background: The World Health Organization recommends that babies be breastfed within the first hour of delivery and that they should exclusively be given breast milk in the first 6 months. The aim of this study was to ascertain the breastfeeding behaviors of refugee Syrian women and to compare their practices with those of the native mothers of the local community. Materials and Methods: This study is a cross-sectional study. The study was conducted in a Southern city in Turkey. The questionnaire was filled by face-to-face interviews. A total of 381 refugee Syrian mothers were compared with 381 native women living in the same community. Results: A significant difference was found between the two groups regarding breastfeeding. The rate of those who initiated breastfeeding within 1 hour after delivery was 61.4% for the Syrians and 71.1% for the Turkish mothers. In addition, the rates of exclusive breastfeeding (28.1 versus 34.1) and continuing breastfeeding for 12 months (55.0 versus 63.8) were lower in the Syrian refugee mothers. Maternal age and educational level were not correlated to the breastfeeding rates. Conclusions: This study is a rare study in which refugee mothers and local mothers were compared. Forced migration and refugee status are negatively associated with breastfeeding behavior.
Editorial: Anxiety, burnout, and stress among healthcare professionals
(Frontiers, 2023) Bayram Değer, Vasfiye
The technology, informatics and social fields are experiencing continuous changes and developments day by day. Such changes and developments influence human life and expand the fields of research. One of these fields is the world of work life. Work life is an area where people spend a significant part of their lives by spending time and effort. The willingness of employees to make quality use of their time and labor significantly affects the efficiency obtained as a result of their work (Burton, 2010). The nature of the work requirements and the quantity of communication with other people in the workplace create challenging situations for employees. In this context, when it comes to health in work life, health workers are seen as an important sample group in terms of researching variables in work life, since their field requires qualified labor force, is vital and is a profession that constantly involves face-to-face interaction with people. The health sector is one of the sectors where employees have the most difficulties due to various factors. The health sector differs from other working environments due to the difficulty of serving patients with severe stress and the fact that employees in this sector often face stressful situations in their daily working environment.
Effect of therapeutic touch on daytime sleepiness, stress and fatigue among students of nursing and midwifery: A randomized sham-controlled trial
(Complementary Therapies in Clinical Practice, 2021) Utli, Hediye; Vural Doğru, Birgül; Şenuzun Aykar, Fisun
Objectives: This study was conducted to assess the effect of therapeutic touch on stress, daytime sleepiness, sleep quality and fatigue among students of nursing and midwifery. Methods: 96 students were randomized into three groups: the therapeutic touch (TT) group, the sham therapeutic touch (STT) group, and the control group. In this randomized sham-controlled study, the TT group was subjected to therapeutic touch twice a week for four weeks with each session lasting 20 min. Results: When the TT group was compared to the STT and control groups following the intervention, the decrease in the levels of stress (p < 0.001), fatigue (p < 0.001) and daytime sleepiness (p < 0.001), and the increase in the sleep quality (p < 0.001) were found to be significant. Conclusion: It was found that TT, which is one form of complementary therapy, was relatively effective in decreasing the levels of stress, fatigue and daytime sleepiness, and in increasing the sleep quality of university students of nursing and midwifery.
Evaluation inflammatory markers of hemogram parameters in primary ovarian insufficiency
(Turkish journal of obstetrics and gynecology, 2020) Demir, Bülent; Sarıdaş Demir, Süreyya; Özkan Karacaer, Kübra; Paşa, Semir; Sılan, Fatma
Objective: In most of primary ovarian insufficiency (POI) cases, etiologic factors have not been fully elucidated. Recent studies have revealed that inflammatory agents play an important role in the etiopathogenesis of POI. Therefore, the aim of this study was to investigate the role of inflammatory markers of hemogram parameters in POI. Materials and Methods: The study compared 47 healthy women and 47 women diagnosed as having POI retrospectively by scanning electronic and written recording systems. Complete blood counts, day-3 hormone profiles levels of all subjects were analyzed. The neutrophil-lymphocyte ratio (NLR), red cell distribution width (RDW), platelet ratio (RPR), platelet lymphocyte ratio (PLR), and mean platelet volume (MPV) mean platelet lymphocyte ratio (MPLR) were calculated from the complete blood count parameters. Results: White blood cell and MPV values, platelet, and lymphocyte counts were significantly higher in the POI patients (p<0.001, p=0.042, p=0.038, p=0.049, respectively), RPR was significantly lower than the control group (p=0.011), but there were no significant differences in hemoglobin, RDW, NLR, PLR, and MPLR (p=0.454, p=0.057, p=0.635, p=0.780, p=0.126, respectively). The neutrophil count of the study group was higher than in the control group (p=0.057). Bivariate correlation analyses showed no correlations between blood parameters and hormone levels. The area under the receiver operating characteristic curve for RPR in POI was 0.652, with a threshold value 0.053, sensitivity=63% and specificity=63. Conclusion: Inflammatory markers of hemogram detected higher in patients with POI then control subjects.
Frequency of clinical alarms in intensive care units and nurses’ sensitivity to them: An observational study
(American Journal of Critical Care, 2021) Baran, Leyla; Ceylan, Burcu; Güneş, Ülkü Yapucu
Background All clinical alarms require nurses to respond even if an intervention is not needed. Nurses are expected to respond appropriately to each alarm and establish priorities among their care practices accordingly. This study was conducted to examine the number and types of clinical device alarms used in intensive care units, the duration of their activation, and nurses’ degree of sensitivity to them. Methods This observational study was conducted in 4 intensive care units in a university hospital in Turkey. A total of 20 nurses (5 from each unit) were observed for a total of 80 hours. The alarms were categorized as valid, false, or technical. Results During the study observation period, the mean number of alarms sounding per hour per bed was 1.8. A total of 144 alarms were recorded, of which 70.8% were valid, 15.3% were false, and 13.9% were technical. The mean duration of alarm activation was 8 minutes for valid alarms, 14 minutes for false alarms, and 53 minutes for technical alarms. Conclusions Nurses’ responses to alarms differ depend-ing on alarm type; for alarms that do not require an emergency intervention, nurses tend to respond late or not at all. (American Journal of Critical Care. 2021;30:186-192).
Health literacy, health perception and related factors among different ethnic groups: a cross-sectional study in southeastern Turkey
(BMC Health, 2021) Yiğitalp, Gülhan; Bayram Değer, Vasfiye; Çifçi, Sema
Background: Low levels of health literacy are associated with increased hospitalization rates, problems regarding the proper intake of medications, poor general health and increased mortality rates. It is a well-known fact that health literacy differs among ethnic groups and ethnic minorities, in particular, are known to have a low level of health literacy. The present study aimed to reveal the levels of health literacy among different ethnic groups and the affecting factors as well as the relationship between health literacy and health perceptions. Methods: This cross-sectional study was carried out with different ethnic groups (Kurdish, Arab, Turkish and Assyrian origin), between 18 and 65 years old in the province of Mardin in Turkey. The study was conducted with a total of 600 people. The European Health Literacy Scale-Turkish Adaptation (EHLS-TR) and Health Perception Scale (HPS) were used for measurement. Descriptive analysis, Mann Whitney U Test, Kruskal Wallis Test and Spearman correlation were used in the data analysis. Results: It was found that 80.7% of the participants had relatively low levels of health literacy. The lowest levels of health literacy were among those of Kurdish origin. There were correlations between sufficient levels of health literacy and several factors including being of Assyrian origin, being 50–65 years old, living in a nuclear family, being a secondary school graduate, having a high financial status, being retired, evaluating one’s own health status as good, obtaining health information from healthcare professionals, preferring to visit a state hospital to seek medical assistance first, smoking and drinking alcohol. A positive correlation was also identified between the levels of health literacy and health perception. Conclusions: It is essential to develop programs to increase health literacy for the public and, in particular, for the ethnic groups that are disadvantaged in many aspects in the context of health literacy.
Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers
(HUMANA PRESS INC, 2015) Aktepe, Necmettin; Koçyiğit, Abdurrahim; Yükselten, Yunus; Taşkın, Abdullah; Keskin, Cumali; Çelik, Hakim
Silver has long been valued as a precious metal, and it is used to make ornaments, jewelry, high-value tableware, utensils, and currency coins. Human exposures to silver and silver compounds can occur oral, dermal, or by inhalation. In this study, we investigated genotoxic and oxidative effects of silver exposure among silver jewelry workers. DNA damage in peripheral mononuclear leukocytes was measured by using the comet assay. Serum total antioxidative status (TAS), total oxidative status (TOS), total thiol contents, and ceruloplasmin levels were measured by using colorimetric methods among silver jewelry workers. Moreover, oxidative stress index (OSI) was calculated. Results were compared with non-exposed healthy subjects. The mean values of mononuclear leukocyte DNA damage were significantly higher than control subjects (p < 0.001). Serum TOS, OSI, and ceruloplasmin levels were also found to be higher in silver particles exposed group than those of non-exposed group (p < 0.001, p < 0.001, p < 0.01, respectively). However, serum TAS levels and total thiol contents of silver exposed group were found significantly lower (p < 0.05, p < 0.001, respectively). Exposure to silver particles among silver jewelry workers caused oxidative stress and accumulation of severe DNA damage.
Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101
(Wiley, 2021) Aktepe, Necmettin; Yukselten, Yunus
The aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.
The intercultural sensitivity levels of primary health care workers in a city in Eastern Turkey
(Journal of the Pakistan Medical Association, 2021) Çifçi, Sema; Bayram Değer, Vasfiye
Objective: To assess intercultural sensitivity levels and related factors of primary healthcare workers providing care to a variety of socio-ethnic groups. Methods: The descriptive cross-sectional study was conducted from March 1, 2019, to February 1, 2020, in the city of Mardin, Turkey, and comprised primary healthcare workers. Data was collected using a questionnaire about socio-demographic characteristics and the Turkish version of the Intercultural Sensitivity Scale. Data was analysed using SPSS 24 software version. Results: Of the 150 subjects approached, 131(87.3%) volunteered to participate. Of them, 80(61%) were females and 51(39%) were males. The largest age group was 25-34 years having 77(59%) subjects. The total mean score of the intercultural sensitivity scale was 73.30±16.7. There was a strong relationship between the subscales of 'Responsibility and Interaction Attentiveness' and 'Respect for Cultural Differences' (p<0.01). Significant difference was found between the sensitivity levels and the job titles of the participants, their status of friendship in different countries, the opportunity to be with people in other cultures for any reason, the status of willingness to work in different cultures and their intercultural sensitivity scale scores (p<0.05). Conclusions: The level of cultural sensitivity of the healthcare professionals was overall moderate. © 2021 Pakistan Medical Association. All rights reserved.

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