PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

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A Comparison of Native and Syrian Immigrant Women Students' Genital Hygiene Behaviors: A Cross-Sectional Study
(Univ Sao Paolo, 2025) Icke, Sibel; Cifci, Sema
Objective: This study aims to compare the genital hygiene behaviors of native and Syrian immigrant women students. Method: This was cross-sectional research. The sample size of the study was determined via power analysis and G*Power software. A total sample size of 330 individuals was equally distributed to both groups. Sociodemographic Characteristics Questionnaire" and the 'Genital Hygiene Behaviors Scale' were used as data collection tools. Results: There was a significant difference between the mean scores of the scales of both student groups (p < 0.05). The mean scores of native students were found to be higher. The variables "how to clean the genital area", "material used for genital hygiene", "washing method of underwear", "pretoilet hand washing habits" and "regular menstruation" were found to be most effective in terms of the mean scores of the Genital Hygiene Behaviors Scale and its subscales for immigrant students. Conclusion: Social security status and nationality were the most influential sociodemographic factors affecting genital hygiene behaviors. It would be beneficial to raise awareness and promote behavioral changes regarding genital hygiene through seminars, conferences, workshops, and similar events targeting all women university students.
Citation - WoS: 3
Citation - Scopus: 3
Aging and cardiac implantable electronic device complications: is the procedure safe in older patients?
(Springer, 2023) Güzel, Tuncay; Aktan, Adem; Kılıç, Raif; Günlü, Serhat; Arslan, Bayram; Arpa, Abdulkadir; Güzel, Hamdullah; Tatlı, İsmail; Aydın, Saadet; Suzan, Veysel; Demir, Muhammed
Background: In this study, we investigated whether there is a higher incidence of cardiac implantable electronic devices (CIED) procedures related complications in older (≥ 75 years) than in younger (< 75 years) patients. Methods: This retrospective cohort study enrolled patients who had undergone CIED procedures (de novo implantation, system upgrade, generator substitution, pocket revision or lead replacement) at two heart centers in Turkey between January 2011 and May 2023. The primary composite endpoint included clinically significant hematoma (CSH), pericardial effusion or tamponade, pneumothorax, and infection related to the device system. Secondary outcomes included each component of the composite end point. Results: The overall sample included 1923 patients (1419 < 75 years and 504 aged ≥ 75 years). There was no difference between the groups in terms of cumulative events defined as primary outcome (3.5% vs. 4.4%, p = 0.393). Infection related to device system was significantly higher in the ≥ 75 age group (1.8% vs. 3.4%, p = 0.034). There was no significant difference between the groups in terms of clinically significant hematoma and pneumothorax (0.7% vs. 0.4%, p = 0.451, 1.4% vs. 1.0%, p = 0.477, respectively). In multivariate model analysis, no association was found between age ≥ 75 years and infection related to the device system. Conclusion: Infection rates were relatively higher in the patient group aged ≥ 75 years. This patient group should be evaluated more carefully in terms of infection development before and after the procedure.
Ameliorative Effects of Agomelatine Against Doxorubicin-Induced Hepatotoxicity
(BMC, 2025) Savas, Hasan Basri; Sozen, Mehmet Enes; Cuce, Gokhan; Batur, Tuba
Drug-induced hepatotoxicity is a significant impediment to the use of doxorubicin, a commonly employed chemotherapeutic agent with established efficacy in cancer treatment. The present study aimed to determine the potential protective effects of agomelatine against doxorubicin hepatotoxicity in rat toxicity models. Thirty-two rats were divided into four groups: control (with saline administration), Doxo (with 40 mg/kg doxorubicin administration), Doxo + Ago20, and Doxo + Ago40 (with 20 and 40 mg/kg agomelatine administration and 40 mg/kg doxorubicin administration). On the day of 14 rats were sacrificed, samples were collected for comparison of immunohistochemical, hematological, and biochemical analysis. There were statistically significant differences between the study groups in terms of immunohistochemical, hematological, and biochemical parameters. Agomelatine administration reduced the TNF-alpha, and caspase-3, which increased by doxorubicin, and reversed levels of oxidative stress markers altered by doxorubicin (p < 0.05). Doxorubicin induces oxidative stress, apoptosis, and hepatotoxicity. Agomelatine may be favored as a primary antidepressant to mitigate hepatic damage induced by doxorubicin.
An Unexpected Cause of Tension Pneumothorax: A Case Report
(John Wiley and Sons Inc, 2025) Talay, M.N.; Sızlanan, A.; Gungor, E.; Kalkan, F.; Orhan, Ö.; Nayir-Buyuksahin, H.
Anatomical Examination of Intraparotid Lymph Nodes in Various Tumors of Parotid Gland Using Magnetic Resonance Imaging
(Elsevier Science Inc, 2025) Selcuk, Mehmet; Dogan, Nadire Unver; Onal, Merih; Batur, Abdussamet; Karabulut, Ahmet Kagan; Fazliogullari, Zeliha
Objective. This retrospective study aimed to anatomically compare the morphometric measurements of intraparotid lymph nodes across different parotid gland tumors and a control group. Study Design. The study used retrospective images of patients admitted to the Department of Otorhinolaryngology over the past 10 years from the Radiology Department archive at Sel,cuk University Medical Faculty Hospital. A total of 160 individuals were included in our study.Long-axis diameter (LAD) and short-axis diameter (SAD) were measured in axial section on magnetic resonance images. Results. In all patients, the mean SAD and LAD were significantly higher on the lesion (+) side compared to the lesion (-) side (P < .05). The mean SAD and LAD were significantly larger on the lesion (+) side compared to the control group (P < .05). Conclusions. In our findings, if the SAD exceeded 3.51 mm and the LAD exceeded 4.85 mm in the control group, it can be considered suspicious in terms of lymph node abnormality, potential pathologic evaluation, and indications for further examination. (Oral Surg Oral Med Oral Pathol Oral Radiol 2025;140:759-768)
Citation - WoS: 1
Citation - Scopus: 1
Ascorbic Acid Exhibits More of a Protective Effect Than Estradiol Against Nephrotoxicity Induced by Malathion in Rats: a Histopathological and Molecular Docking Study
(TUBITAK Scientific & Technological Research Council Turkey, 2025) Alhilal, Mohammad; Salem, Mahmoud E. L. S. A. Y. E. D. M. O. H. A. M. E. D.; Ali Albakoush, Ahmed; Alhilal, Suzan; Farag, Basant; Gomha, Sobhi M.
Background/aim: Despite the known harmful effects associated with malathion toxicity in various organs, it continues to be widely used for plant protection and insect control. This study is the first to compare the protective effects of estradiol and ascorbic acid against malathion-induced nephrotoxicity through histopathological assessment and molecular docking analyses. Materials and methods: This study was conducted using 20 female albino rats that were distributed into sham, malathion, malathion + estradiol, and malathion + ascorbic acid groups. Nephrotoxicity was induced by daily treatment with malathion and the effects of estradiol and ascorbic on nephrotoxicity were evaluated. After 4 weeks of treatment, the animals were sacrificed and the kidneys were examined following hematoxylin and eosin (H&E) staining. Histopathology results were supported by molecular docking studies of estradiol and ascorbic acid against a target protein (PDB ID: 2YMX), the peptide inhibitor Fab408 inhibiting acetylcholinesterase (AChE). The inhibition of AChE is the primary mechanism of the toxic effects of malathion. Results: Histopathological examination revealed a notable elevation (p < 0.001) in degeneration and necrosis within the tubular epithelium and interstitial nephritis in the malathion group compared to the sham group. Daily administration of estradiol and ascorbic acid resulted in a notable reduction (p = 0.0022) in the severity of these histopathological changes in the malathion + estradiol and malathion + ascorbic acid groups compared to the malathion group. Of these, the most significant decreases were observed in the malathion + ascorbic acid group. Docking studies of these compounds against the selected protein (PDB ID: 2YMX) revealed promising binding scores. Ascorbic acid exhibited the highest docking score (-6.44 kcal/mol), indicating a favorable binding interaction with this protein. Conclusion: Estradiol and ascorbic acid exert protective effects against malathion-induced nephrotoxicity, whereas ascorbic acid showed superior efficacy compared to estradiol. This result was further supported by molecular docking studies.
Citation - WoS: 1
Citation - Scopus: 2
Association Between the Triglyceride-Glucose Index and Contrast-Induced Nephropathy in Chronic Total Occlusion Patients Undergoing Percutaneous Coronary Intervention
(Bmc, 2025) Soner, Serdar; Aktan, Adem; Kilic, Raif; Guzel, Hamdullah; Tastan, Ercan; Oksul, Metin; Guzel, Tuncay
Objective The triglyceride glucose (TyG) index is a biomarker of insulin resistance and is associated with an increased risk of cardiovascular events. Contrast-induced nephropathy (CIN) is an important complication that causes poor outcomes in patients undergoing percutaneous coronary intervention (PCI). In this study, we aimed to investigate the relationship between the TyG index and CIN and mortality in patients who underwent PCI due to chronic total coronary occlusion (CTO). Methods Two hundred eighteen individuals from three separate medical centers who underwent procedural PCI between February 2010 and April 2012 and had a CTO lesion in at least one coronary artery were recruited. According to the TyG index, patients were divided into two groups. Patients with a TyG index >= 8.65 were included in Group 1, and patients with a TyG index < 8.65 were included in Group 2. Patients were followed up for 96 months. The main outcome was the development of CIN and mortality. Results The mean age of the patients (65.8 +/- 10.94 vs. 61.68 +/- 11.4, P = 0.009), diabetes mellitus (60 [44.8%] vs. 11 [13.1%], P < 0.001), and dyslipidemia rates (52 [38.8%] vs. 21 [25%], P = 0.036) were higher in group 1. In multivariable logistic regression analysis, it was seen that age (OR = 1.04, 95% CI = 1.01-1.08, P = 0.020), chronic kidney disease (OR = 2.34, 95% CI = 1.02-5.33, P = 0.044), peripheral artery disease (OR = 5.66, 95% CI = 1.24-25.91, p = 0.026), LVEF (OR = 0.95, 95% CI = 0.92-0.99, P = 0.005), LDL cholesterol levels (OR = 1.00, 95%CI = 1.00-1.02, P = 0.024) and TyG index (OR = 2.17, 95% CI = 1.21-3.89, P = 0.009) were independent predictors of the development of CIN. Conclusion Our study demonstrates a correlation between the TyG index and the prevalence of CIN in patients with CTO undergoing PCI. Adding the TyG index to the routine clinical evaluation of patients with CTO undergoing PCI may help protect patients from the development of CIN.
Association of Gla-Rich Protein (GRP) With Inflammatory Markers in Critically Ill Patients: A Cross-Sectional Observational Study
(MDPI, 2025) Eygi, Elif; Bayrakci, Sinem; Bayrakci, Onur; Ayhan, Nazire Ates; Atlas, Ahmet; Kilinc, Metin; Dokuyucu, Recep
Objectives: Gla-rich protein (GRP), a vitamin K-dependent protein, has been increasingly recognized for its dual role in modulating inflammation and inhibiting pathological calcification. Despite its emerging importance in chronic conditions, limited evidence exists regarding its behavior during acute critical illness. This study aimed to investigate the association between GRP, systemic inflammatory markers, oxidative stress (via total thiol oxidation-reduction ratio, TORR), and calcium metabolism in critically ill patients. Materials and Methods: This cross-sectional observational study included 93 critically ill patients admitted to the intensive care unit (ICU) and 60 age- and sex-matched non-critically ill volunteers. Serum GRP levels were measured using ELISA. Other biomarkers including TORR, C-reactive protein (CRP), procalcitonin (PCT), white blood cell count (WBC), immature granulocytes (IGs), and serum calcium were also analyzed. Pearson's correlation, multivariate linear regression, and ROC analysis were performed to assess the relationships among GRP and biochemical markers, as well as their capacity to differentiate ICU patients from controls. Results: GRP, TORR, CRP, PCT, WBC, IGs, and ferritin levels were significantly elevated in ICU patients compared to the control group, whereas serum calcium levels were markedly reduced (all p < 0.05). GRP levels demonstrated moderate positive correlations with WBC (r = 0.47), neutrophils (r = 0.51), TORR (r = 0.42), CRP (r = 0.30), and IGs (r = 0.46), and a strong negative correlation with calcium (r = -0.63). In multivariate regression, TORR, CRP, WBC, IGs, PCT, and calcium levels showed significant correlations with GRP levels in univariate analysis. ROC analysis revealed that CRP had the highest discriminatory power (AUC = 0.88; 95% CI: 0.82-0.94), followed by TORR (AUC = 0.79; 95% CI: 0.71-0.86), GRP (AUC = 0.76; 95% CI: 0.68-0.84), and IGs (AUC = 0.77; 95% CI: 0.69-0.85), for distinguishing ICU patients from non-critically ill individuals. Conclusions: Our findings demonstrated that GRP is significantly associated with systemic inflammation, oxidative stress, and calcium metabolism disturbances in critically ill patients. The combined evaluation of GRP and TORR may enhance the understanding of inflammatory and oxidative mechanisms in acute critical illness. Although this study did not assess patient outcomes, these biomarkers could serve as promising candidates for future prognostic research in ICU settings.
Atypical Pneumonia Mimicking Childhood Interstitial Lung Disease With Imaging Findings: A Case Report
(John Wiley and Sons Inc, 2025) Öncül, M.A.; Nayir-Buyuksahin, H.; Unay, E.; Orhan, Ö.; Çıkman, G.; Demirbuga, A.; Özbek, M.N.
Citation - WoS: 7
Citation - Scopus: 20
Automatic Detection of Brain Tumors With the Aid of Ensemble Deep Learning Architectures and Class Activation Map Indicators by Employing Magnetic Resonance Images
(Elsevier, 2024) Turk, Omer; Ozhan, Davut; Acar, Emrullah; Akinci, Tahir Cetin; Yilmaz, Musa
Today, as in every life-threatening disease, early diagnosis of brain tumors plays a life-saving role. The brain tumor is formed by the transformation of brain cells from their normal structures into abnormal cell structures. These formed abnormal cells begin to form in masses in the brain regions. Nowadays, many different techniques are employed to detect these tumor masses, and the most common of these techniques is Magnetic Resonance Imaging (MRI). In this study, it is aimed to automatically detect brain tumors with the help of ensemble deep learning architectures (ResNet50, VGG19, InceptionV3 and MobileNet) and Class Activation Maps (CAMs) indicators by employing MRI images. The proposed system was implemented in three stages. In the first stage, it was determined whether there was a tumor in the MR images Tumor) were detected from MR images (Multi-class Approach). In the last stage, CAMs of each tumor group were created as an alternative tool to facilitate the work of specialists in tumor detection. The results showed that the overall accuracy of the binary approach was calculated as 100% on the ResNet50, InceptionV3 and MobileNet architectures, and 99.71% on the VGG19 architecture. Moreover, the accuracy values of 96.45% with ResNet50, 93.40% with VGG19, 85.03% with InceptionV3 and 89.34% with MobileNet architectures were obtained in the multi-class approach.
Bioactive Profile, Anticarcinogenic, Antimicrobial, Antidiabetic Effects, and in Silico Pharmacokinetic Properties of Astragalus Elatus
(Academic Press inc Elsevier Science, 2025) Ercan, Leyla; Akan, Hasan; Caliskan, Cemile Gunbegi
Because of the numerous useful components they contain, plants are significant resources that are being explored for their varied purposes in industry, food, and medicine. Recently, particularly endemic species have gained attention with the identification of their different ingredients and characteristics and the exploration of their precious features. This research assessed the endemic species Astragalus elatus's bioactive components utilizing UHPLC-Orbitrap (R)-HRMS, its antibacterial qualities utilizing the disc diffusion method, its cytotoxic impact on cervical cancer utilizing the MTT method, and its apoptosis effect by annexin V methods. Additionally, Astragalus elatus's antidiabetic activity was revealed by in silico and in vitro examinations of its inhibitory effect on the enzymes alpha-Amylase and alpha-Glucosidase. Consequently, it was discovered that A. elatus can be utilized in the treatment of cervical cancer and has effective cytotoxic and apoptotic effects on HeLa cells. Additionally, it was detected that A. elatus possesses antibacterial and anti-diabetic properties. Compared to gram-negative bacteria, A. elatus had a greater inhibitory effect on gram-positive bacteria. The pharmacokinetically significant characteristics and their tendency for chemical reactions of this plant's primary phenolic compounds were identified by utilizing DFT, molecular docking, and ADME/T.
Citation - WoS: 3
Citation - Scopus: 3
Biological Evaluation and Molecular Docking Studies of Novel Aza-Acyclic Nucleosides as Putative Antimicrobial, Anticancer, and Antioxidant Agents
(BioMed Central Ltd, 2025) Alhilal, M.; Alhilal, S.; Gomha, S.M.; Farag, B.; Sabancilar, I.; Ouf, S.A.
This study aimed to synthesize new aza-acyclic nucleosides (aza-acyclovir) and evaluate the efficacy of these synthetic compounds as potential antimicrobial, anticancer, and antioxidant agents. We prepared two novel aza-acyclic nucleosides via two reactions. The first reaction involved trichloroisocyanuric acid and dibenzosulphonyl diethylamine, and the second reaction involved trichloroisocyanuric acid and diethanolamine. We then used one-dimensional nuclear magnetic resonance (NMR) spectroscopy, two-dimensional NMR spectroscopy, infrared spectroscopy, and mass spectrometry to determine the structures of the resulting compounds. In this regard, we first tested the antimicrobial activity of these compounds against various bacteria, including Bacillus cereus, B. subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa, and against fungal pathogens, including Aspergillus fumigatus, Candida tropicalis, and Alternaria solani. Next, the precise mode for the interaction between synthesized aza-acyclic nucleosides and the target protein 8HQ5 was elucidate using molecular docking analysis. Subsequently, we tested the synthesized compounds for putative anticancer activity at different concentrations (i.e., 12.5, 25, 50, 100, and 200 µg/mL) against A549 cell (Human epithelial lung carcinoma) and human umbilical vein endothelial cell (HUVEC) lines. In addition, compounds antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl-based and cupric reducing antioxidant capacity-based methods at different concentrations (i.e., 31.25, 62.5, 125, 250, and 500 µg/mL). Results revealed that both aza-acyclic nucleosides inhibited both bacterial and fungal strains, although toxicity toward bacterial strains was generally greater than toward fungal strains. We also observed that the molecular docking results were consistent with the results of in vitro antimicrobial assessments. Further, both aza-cyclic nucleosides exhibited cytotoxic effects against both the A549 cell and HUVEC lines. Despite exhibiting lower radical scavenging activity than ascorbic acid (an antioxidant compound used as a standard), Compound 1 from the novel synthetic aza-acyclic nucleosides showed a higher reduction capacity, which was dose-dependent. Overall, we report newly synthesized compounds that show promising antimicrobial, anticancer, and antioxidant effects. © 2025 Elsevier B.V., All rights reserved.
Citation - WoS: 1
Citation - Scopus: 1
Black box in overactive bladder: Central sensitization and its relationship with urinary symptom severity and quality of life
(Wiley, 2024) Toprak Şelenay, Ceyda; Altay, Hafize; Bülbül, Saliha Beste; Oskay, Kemal
Aim: To examine central sensitization (CS), and to investigate the relationship between CS, and urinary symptom severity, and quality of life (QoL) in women with overactive bladder (OAB). Materials and methods: A total of 144 women with OAB included the study. CS with the Central Sensitization Inventory (CSI), urinary symptom with the Overactive Bladder Questionnaire-Version 8 (OAB-V8), bladder diary and Patients' Perception of Intensity of Urgency Scale (PPIUS) and QoL with the King's Health Questionnaire (KHQ) were assessed. Results: It was found that 47.9% (n = 69) of women with OAB had CS. It was observed that the CSI score was related to the OAB-V8 score (ρ = 0.327; p < 0.001) and the average number of voids/day (ρ = 0.291; p < 0.001). Additionally, urgency severity was higher in women with OAB with CS than in women with OAB without CS (p = 0.006). There was a relationship between the CSI score and KHQ-incontinence impact (ρ = 0.250; p = 0.012), KHQ-personal relationship (ρ = 0.253; p = 0.002), KHQ-sleep/energy (ρ = 0.180; p = 0.031), KHQ-emotional state (ρ = 0.310; p < 0.001) and KHQ-severity measurement scores (ρ = 0.391; p < 0.001). Conclusion: In this study, it was observed that the majority of women with OAB had CS. It was found that more severe symptoms of CS were associated with worse urinary symptom severity and QoL in these patients. It may be beneficial to evaluate CS in the management of OAB and to consider CS when determining treatment strategies.
Citation - WoS: 1
Citation - Scopus: 1
Body Image, Self-Esteem, and Attitudes Toward Sexuality in Older Adults With Chronic Illnesses
(Springer/Plenum Publishers, 2025) Utli, Adil; Ersu, Nilay Filoglu; Ozbay, Hediye
This study aimed to examine the effect of the association between body image and self-esteem levels on attitudes toward sexuality in older adults with chronic illnesses. The research was descriptive, cross-sectional, and correlational. The study sample comprised 1,004 people over the age of 65 who visited family health centers in a province in the east of T & uuml;rkiye. The Older Person's Description Form, the Body-Cathexis Scale, the Rosenberg Self-Esteem Scale Short Form, and the Sexual Attitude Scale for Elderly People were used for data collection. For the data analysis, Pearson's r correlation test, simultaneous multiple linear regression, and binary logistic regression were employed. The mean age of the participants was 69.97 +/- 5.74 years, and 66.9% were between 65 and 74 years of age. Furthermore, 53.8% of the participants were male and 56.2% had two chronic illnesses. It was found that the participants' age, body image, and self-esteem explained 84% of the total variance in their permissive attitudes toward sexuality. Furthermore, as the age of the older adults with chronic illnesses increased, there were negative effects on their permissive attitudes toward sexuality. However, as their body image and self-esteem levels improved, there was a positive effect on their permissive attitudes toward sexuality. These findings will be of benefit in encouraging and developing sexual health in older adults with chronic illnesses.
Can Inflammatory Indices Predict Myringosclerosis
(Saudi Med J, 2025) Sari, Neslihan; Savas, Hasan Basri; Ugras, Seda; Durucu, Cengiz
Objectives: To examine the relationships between the neutrophil-to-lymphocyte ratio (NLR), the platelet-to-lymphocyte ratio (PLR), and the systemic immune-inflammation index (SII) in myringosclerosis after ventilation tube insertion (VTI). Methods: We conducted this descriptive case-control study between March 2022 and September 2024. A total of 75 children aged 6 to 12 years with a history of VTI were enrolled. Myringosclerosis+ (n=43) included children with myringosclerosis, while Myringosclerosis-(n=32) included children without myringosclerosis. Preoperative values for NLR, PLR, and SII were collected by hematological testing and calculations. Demographic factors, prognostic risk factors and discrimination power of parameters were determined by statistical methods. Results: Compared to the myringosclerosis-, the myringosclerosis+ had significantly higher NLR, PLR, and SII values (p=0.00, p=0.04, and p=0.01, respectively). Receiver operating characteristic (ROC) curve area of NLR is 0,76 (95% CI: 0.65-0.87, p=0.00). Area under curve of SII is 0,62 (95%CI: 0.57-0.81, p=0.01). Neutrophil-to-lymphocyte ratio affects myringosclerosis development at 1.34 with sensitivity=0.63, specificity=0.78. Neutrophilto-lymphocyte ratio(NLR) and systemic immune inflammation index(SII) show myringosclerosis development at different powers of predictivity. The ROC area of NLR is highest of all parameters but is moderately predictive (<0.80). It is slight for SII. The NLR may be a marker of prediction of myringosclerosis OR=0.182 (95%CI:0.067-0.496). Conclusion: Neutrophil-to-lymphocyte ratio and SII are systemic inflammatory prognostic markers in myringosclerosis, and elevated NLR values are a predictor of myringosclerosis with modest affect. Other parameters should be sought in myringosclerosis development.
Citation - WoS: 2
Citation - Scopus: 2
Can Quercetin Reduce Arsenic Induced Toxicity in Mouse Balb/C 3t3 Fibroblast Cells ? A Study Involving in Vitro, Molecular Docking, and Adme Predictions
(Bmc, 2025) Unsal, Velid; Keskin, Cumali; Oner, Erkan
This study aimed to investigate the protective effect of quercetin against arsenic-induced oxidative damage, inflammation, and apoptosis in mouse BALB/c 3T3 fibroblast cells (NIH-3T3). Arsenic at different concentrations of 0.05 mu M (low), 0.5 mu M (medium), 10 mu M (high) doses were used to induce toxicity, while 120 mu m quercetin was used for treatment. MTT and LDH analyses were performed to determine the effect of arsenic and quercetin on cell viability, while oxidative stress markers and antioxidant enzyme activities were measured by spectrophotometric method. TNF-alpha and IL-1 beta levels were measured by the ELISA method, Autodock programs were used for molecular docking studies. In addition, computer-based analyses of quercetin and succimer molecules were performed using SwissADME web tools. TNF-alpha (PDB ID: 2AZ5), IL-1 beta (PDB ID: 1ITB), Caspase3 (PDB ID: 2XYG), Bax (PDB ID: 4S0O), SOD (PDB ID:1CBJ), GSH-Px (PDB ID: 1GP1) and Bcl-2 (PDB ID: 1G5M) crystal structures were obtained from the Protein Data Bank. Bax and Bcl-2 levels of apoptotic genes and mRNA expression levels of Caspase-3 activity were measured using the QRT-PCR technique. TUNEL staining was performed to determine DNA fragmentations, while DAPI staining was done to visualise nuclear modifications. Quercetin has been found to significantly reduce oxidative stress, inflammation, and apoptosis in cells and exert anti-apoptotic effects. Molecular docking studies revealed quercetin shows good binding affinity with molecules with SOD, GSH-Px, Bax, Bcl-2, Caspase-3, TNF-alpha and IL-1 beta structures, and has been observed to bind with Bax and Bcl-2 with molecular docking scores of -7.5 and - 7.7 kcal/mol, respectively. These findings are supported by results showing that quercetin is effective in anti-apoptotic and anti-inflammatory processes in arsenic-induced cells under in vitro conditions. In addition, when ADME values are examined, it can be considered that quercetin is a useful and effective candidate compound in reducing arsenic toxicity, considering its higher synthetic accessibility score, better pharmacokinetic properties, and good biological transition and interaction capacities compared to succimer.
Citation - WoS: 10
Citation - Scopus: 11
Carbamazepine-induced renal toxicity may be associated with oxidative stress and apoptosis in male rat
(Taylor & Francis Online, 2021) Erdem Güzel, Elif; Kaya Tektemur, Nalan; Tektemur, Ahmet; Etem Önalan, Ebru
Carbamazepine (CBZ) is the antiepileptic drug used in epilepsy and some psychiatric disorders. Besides its widely used, many adverse effects have been reported including hematotoxicity, hepatotoxicity, endocrine disorders, and testicular damages due to oxidative stress. However, the role of CBZ on renal toxicity is not fully known. In this study, we attempted to explain the connected mechanisms by focusing on the metabolism of CBZ-induced renal toxicity in rats. Twenty male Wistar-Albino rats were randomized into 2 groups (n = 10); control (1 mL/day distilled water, orally) and CBZ (25 mg/kg/day CBZ, orally) groups. After 60 days, TAS (total oxidant status) and TOS (total oxidant status) levels, histopathological features, some genes involved in apoptosis, 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, and apoptotic cells were assessed of kidney tissue. The oxidative stress index (OSI) was measured from TAS and TOS levels. TOS levels and OSI significantly increased, while TAS levels decreased in the CBZ group relative to the control group. Histopathological observations, Caspase-3 (Casp3), Poly [ADP-ribose] polymerase-1 (PARP-1), 8-OHdG immunoreactivities, and apoptotic cells markedly raised in the CBZ group compared with the control group. Also, mRNA expression of Cytochrome c (Cytc) and CASP3 significantly increased in the CBZ group compared to the control group. In conclusion, long-term use of CBZ may promote renal damage in rats by inducing oxidative stress and apoptosis.
Cardiac Autonomic Control Reflects Sympathovagal Changes Associated With Withholding Urination
(Wiley, 2025) Alabdo, Ahmad; Oflazoglu, Basak; Kus, Mehmet Mustafa; Cakan, Pinar; Ugras, Seda; Yildiz, Sedat
IntroductionIncreased volume of the urinary bladder causes urge to urinate and constricts the sphincter by increasing the tonus of the sympathetic nervous system (SNS). Activity of the autonomic nervous system (or sympatho-vagal balance) can be assessed by heart rate variability (HRV). Current study aimed to test in healthy young male participants whether HRV technique reflects increased sympathovagal balance during bladder distention as revealed by strong urge to urinate.Materials and MethodsA total of young and apparently healthy 11 males agreed to participate to the current study. They were asked to provide 5-min continuous electrocardiogram (ECG) recordings in supine position for determination of HRV on two occasions, i.e. before and approximately for 3 1/2 hours after withholding urination. HRV parameters included heart rate (HR, per min), total power (TP, ms2), and standard deviation of RR intervals (SDNN, ms); percentage of successive RR intervals that were different >= 50 ms (pNN50), low frequency (LF) and high frequency (HF) bands and their normalized units (LFnu and HFnu, respectively) and their ratios (LF/HF). Half of the students provided samples on both occasions and those data were compared by paired t-test following log10 transformation.ResultsWithholding urination did not change HR (from 80.0 +/- 4.1 to 84.5 +/- 3.1 per min, p = 0.135) but decreased total power (from 2692 +/- 802 to 1605 +/- 357 ms2, p = 0.008), SDNN (from 50.4 +/- 5.8 to 38.7 +/- 3.8 ms, p = 0.007) and pNN50 (from 15.6 +/- 3.3 to 5.9 +/- 2.6%, p = 0.016) and increased LFnu (from 57.3 +/- 5.3 to 65.1 +/- 4.5, p = 0.029) and LF/HF ratio (from 1.77 +/- 0. 73 to 2.42 +/- 0.47, p = 0.047).ConclusionsIncreased LF/HF together with decreased SDNN and pNN50 suggest that HRV successfully reflects increased sympathetic tonus over the parasympathetic to help accommodate the urine in the bladder. Moreover, it seems that HRV can be used to assess sympathovagal changes non-invasively during voiding. Additionally, it appears that in all HRV measurements, participants should not have postponed their urination during recording.
Citation - WoS: 3
Citation - Scopus: 6
Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring
(Taylor & Francis Online, 2020) Erdem Güzel, Elif; Nalan Kaya, Ahmet Tektemur, Nazife Ulker, Ahmet Yardimci, Ramazan Fazil Akkoc, Sinan Canpolat & Ibrahim Enver
Prenatal tobacco-smoke exposure negatively affects the reproductive functions of female offspring and oxidative stress plays a major role at this point. Alpha-lipoic acid (ALA), well known as a biological antioxidant, has been used as a nutritional supplement and as a therapeutic agent in the treatment of certain complications during pregnancy. We aimed to investigate the effects of maternal tobacco-smoke exposure and/or ALA administration on puberty onset, sexual behavior, gonadotrophin levels, apoptosis-related genes, apoptotic cell numbers and oxidative stress markers in the adult female rat offspring. Sprague-Dawley rats were divided into four groups; control, tobacco smoke (TS), TS+ALA and ALA groups. Animals were exposed to TS and/or ALA for 8 weeks before pregnancy and throughout pregnancy. All treatments ended with birth and later newborn female rats were selected for each experimental group. The experiment ended at postnatal day 74-77. Maternal tobacco smoke advanced the onset of puberty in the female offspring of the TS group (p < 0.05). In all treatment groups; the mean number of anogenital investigations and lordosis quality scores showed a decline, serum luteinizing hormone levels significantly increased (p < 0.05) and several histopathological changes in ovaries were observed compared to the control group. In addition, an increase in apoptotic marker levels and apoptotic cell numbers was detected in the ovaries of all treatment groups. Decreased TAS and increased TOS levels were detected in all treatment groups compared to control. These findings suggested that maternal tobacco smoke and/or ALA administration may be leading to the impaired reproductive health of female offspring. Abbreviations: ALA: alpha-lipoic acid; LH: luteinizing hormone; FSH: follicle-stimulating hormone; TAS: total antioxidant status; TOS: total oxidant status; Apaf1: apoptotic protease-activating factor 1; Casp3: caspase 3; Casp9: caspase 9; CF: cyst follicles; 4-HNE: 4-Hidroxynonenal; 8-OHdG: 8-hydroxydeoxyguanosine; TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine-biotin nick end labeling; ROS: reactive oxygen species; GnRHR: gonadotropin-releasing hormone receptor; HPG: hypothalamic-pituitary-gonadal; AMPK: AMP-activated protein kinase; ELISA: enzyme-linked immunosorbent assay; cDNA: complementary DNA; qPCR: quantitative real-time PCR; FC: follicular cysts; PF: primary follicle; SF: secondary follicle; GF: graafian follicle; CL: corpus luteum; DF: degenerated follicle; AF: atretic follicle.
Citation - WoS: 3
Citation - Scopus: 3
Chronic Maternal Tobacco Smoke Exposure and/or Alpha-Lipoic Acid Treatment Causes Long-Term Deterioration of Testis and Sexual Behavior in Adult Male Rats
(Elsevier, 2020) Yardimc, Ahmet; Ramazan Fazil Akkoc, Ahmet Tektemur, Nazife Ulker, Nalan Kaya Tektemur, Elif Erdem Güzel, Sinan Canpolat, Ibrahim Enver Ozan
Background: Tobacco use during pregnancy is known to have several negative effects on the offspring's reproductive health in the long term. The use of alpha-lipoic acid (ALA) as a dietary supplement during pregnancy has increased greatly in recent years and has been known to have positive effects on various pregnancy outcomes including miscarriage, diabetic embryopathy, preterm delivery, and congenital malformations. Aim: To evaluate the effects of tobacco smoke exposure (TSE) on sexual behavior, reproductive parameters, and testicles in adult male rats and to reveal the possible role of ALA administration on these parameters. Methods: Pregnant rats (n = 7 per group) were treated with tobacco smoke (TS), ALA (20 mg/kg), and TS + ALA for a total of 11 weeks. The following parameters were compared with 8 control rats: puberty parameters, sexual behavior; levels of serum gonadotropins and testosterone, total antioxidant status, and total oxidant status; the expression of the apoptotic protease-activating factor-1 and caspase 9 mRNA levels in the testis; and assessment of immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling assay of testis. Main Outcome Measure: Sexual behavior, changes in puberty parameters, and hormonal and genetic alterations were the outcomes analyzed in this study. Results: Maternal TSE caused a significant decrease in the number of intromissions compared to the control group. Similarly, ALA decreased erectile function in sexual behavior by decreasing the number of intromissions and intromission ratio in the ALA group compared to the control group. In addition, TSE and ALA treatment caused an impairment of some consummatory sexual behaviors. Also, in parallel with this inhibitory effect, the age of pubertal onset was significantly delayed in the TS + ALA group compared to other groups. Also, histopathological changes in testicular tissue, oxidative stress markers, apoptotic index, and mRNA levels of apoptosis-related genes increased in all treatment groups. Clinical Implications: The use of ALA and/or tobacco products during pregnancy may adversely affect the reproductive health of male newborns in the long term. Strengths & Limitations: To the best of our knowledge, this study is the first to show the effects of maternal ALA treatment and/or TSE on the sexual behavior and reproductive parameters in male rats; however, the study is based on an animal model, and the present findings partially reflect the characteristics of human sexual behavior. Conclusion: Maternal TSE and/or ALA treatment may impair sexual behavior in adulthood in male rats because of testicular damage caused by oxidative stress during gonadal development. Yardimci A, Akkoc RF, Tektemur A, et al. Chronic Maternal Tobacco Smoke Exposure and/or Alpha-Lipoic Acid Treatment Causes Long-Term Deterioration of Testis and Sexual Behavior in Adult Male Rats. J Sex Med 2020;17:1835–1847.

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