PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

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Comparison of Different Fixatives Effects in Histochemical Stainings of Peripheral Nerve Tissue
(Cellular and Molecular Biology Association, 2024) Dörtbudak, M.B.; Demircioğlu, M.; Şeker, U.; Demircioğlu, İ.; Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
A pathological condition in the peripheral nerve tissue, which provides the connection between the organism and the external environment, negatively affects the standard of living. The nerve tissue histotechnology is of serious importance both for scientific studies and for clinical diagnosis. The fixation, which is one of the leading procedures for histological examination of tissues, aims to preserve tissue morphology. Another essential part of the histological examination is staining process. This study, it was aimed to determine the fixative that provides optimal histological appearance in peripheral nerve tissue. Therefore, various histochemical stainings of tissues fixed with some fixatives used in practice were compared. Sciatic nerves from each rat (n=7) used in the study were fixed with different fixatives and histochemical staining was performed. In histological examination, cellular (nucleus-cytoplasm) and intercellular morphological details, staining intensity and distribution were evaluated. At the end of the study, formaldehyde was found to be the most ideal fixing agent for all stains. Although Bouin and Carnoy fixatives differed according to the staining type, their fixation quality was similar in general. Glutaraldehyde did not give as good results as other fixatives in all stainings. This study is an important technical reference for clinical and experimental studies. © 2024 Cellular and Molecular Biology Association. All rights reserved.
Citation - WoS: 1
Citation - Scopus: 2
Green Synthesis of pleurotus Eryngii-derived Nanomaterials for Phytopathogen Control
(Wiley-v C H verlag Gmbh, 2024) Acay, Hilal; Guney, Inci Guler; Yildirim, Ayfer; Dervis, Sibel; Dereli, Elif; 09.03. Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Growing concerns over the human health and environmental impacts of conventional fungicides, coupled with the escalating challenge of microbial resistance, have fueled the search for sustainable biocontrol strategies against plant pathogens. This study reports, for the first time, the green synthesis and characterization of a novel, eco-friendly nanomaterial, designated Pleurotus eryngii-Lecithin-Chitosan Nanomaterial (PEELCN), derived from P. eryngii extract (PEE), lecithin (L), and chitosan (C). The structural attributes of PEELCN were elucidated using Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), X-ray Diffraction (XRD), and zeta potential measurements, confirming the successful formation of a stable and uniform nanostructure. The antifungal activity of PEELCN, and PEE, was assessed against five economically important phytopathogenic fungi: Neoscytalidium dimidiatum, Alternaria alternata, Verticillium dahliae, Bipolaris sorokiniana, and Fusarium oxysporum. Both PEE and PEELCN exhibited significant inhibitory effects on the mycelial growth of V. dahliae, B. sorokiniana, and N. dimidiatum, with varying degrees of efficacy. The differential antifungal activity suggests a species-specific mode of action. The findings highlight the promising potential of PEELCN as a sustainable, biocompatible, and cost-effective nanofungicide for the management of plant diseases, with the potential for development into a commercially viable biofungicide for sustainable agriculture.
Citation - WoS: 1
Citation - Scopus: 1
Phytochemical Profile, Antimicrobial, Antioxidant, Anti-Xanthine Oxidase, and Anti-Elastase Activities of Centaurea Hyalolepis: an in Silico and in Vitro Analysis
(Wiley-v C H verlag Gmbh, 2025) Ercan, Leyla; Caliskan, Cemile Gunbegi; Akan, Hasan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study aimed to examine the antibacterial, antioxidant, and enzyme activity of Centaurea hyalolepis, as well as the total phenolic and carotenoid contents, and volatile and phenolic components. Gas chromatography-mass spectrometry was used for the volatile component analysis of C. hyalolepis, while liquid chromatography-mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, cupric ion-reducing antioxidant capacity, and N,N-dimethyl-p-phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that C. hyalolepis has antioxidant properties and is a highly efficient antibacterial agent against Bacillus megaterium, Pseudomonas aeruginosa, Bacillus subtilis subsp. spizizenii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, and Candida albicans. Additionally, C. hyalolepis contains many volatile compounds, such as 2-methyl propanoic acid, 3,5-octadien-2-one, 2,6-dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta-cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7-glucuronide, quinic acid, and 4-hydroxybenzoic acid. Moreover, C. hyalolepis was shown to inhibit xanthine oxidase and elastase.
Citation - Scopus: 1
Radiologic Severity Index Can Be Used To Predict Mortality Risk in Patients With Covid-19
(Turkish Assoc Tuberculosis & Thorax, 2024) Sahutoglu, Elif; Kabak, Mehmet; Cil, Baris; Atay, Kadri; Peker, Ahmet; Guler, Suekran; Sahutoglu, Tuncay; Department of Internal Medical Sciences / Dahili Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Introduction: Pneumonia is a common symptom of coronavirus disease-2019 (COVID-19), and this study aimed to determine how analyzing initial thoracic computerized-tomography (CT) scans using semi-quantitative methods could be used to predict the outcomes for hospitalized patients. Materials and Methods: This study looked at previously collected data from adult patients who were hospitalized with a positive test for severe acute respiratory syndrome coronavirus-2 and had CT scans of their thorax at the time of presentation. The CT scans were evaluated for the extent of lung involvement using a semi-quantitative scoring system ranging from 0 to 72. The researchers then analyzed whether CT score could be used to predict outcomes. Results: The study included 124 patients, 55 being females, with a mean age of 46.13 years and an average duration of hospitalization of 11.69 days. Twelve patients (9.6%) died within an average of 17.2 days. The non-surviving patients were significantly older, had more underlying health conditions, and higher CT scores than the surviving patients. After taking age and comorbidities into account, each increase in CT score was associated with a 1.048 increase in the risk of mortality. CT score had a good ability to predict mortality, with an area under the curve of 0.857 and a sensitivity of 75% and specificity of 85.7% at a cut-off point of 25.5. Conclusion: Radiologic severity index, which is calculated using a semi-quantitative CT scoring system, can be used to predict the mortality of COVID-19 patients at the time of their initial hospitalization.
Citation - WoS: 1
Citation - Scopus: 1
Rafiq Syndrome: Old Variant in Man1b1 Gene and Some New Phenotypic Features
(Iranian Child Neurology Soc, 2025) Ozgun, Nezir; Guvenc, Merve Saka; Department of Internal Medical Sciences / Dahili Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Rafiq syndrome is a congenital disorder of glycosylation type II that develops due to mutations in the Mannosidase Alpha Class 1B Member 1 (MAN1B1) gene encoding alpha 1,2-mannosidase. In the literature, 45 patients have been reported to date. This study presents a patient with some phenotypic traits that differ from previously reported patients with Rafiq syndrome.Since the patient was not diagnosed despite detailed examinations, whole exome sequencing was performed. The patientss' homozygous c.1000 C>T (p.Arg334Cys) pathogenic variant was detected in the MAN1B1 gene (NM_016219.5), which was consistent with Rafiq syndrome. Our patient's clinical findings were mainly similar to those of previously reported patients. However, our patient had feeding difficulty that started to improve after the fifth month and persistent hyperekplexia . Feeding difficulty and hyperekplexia concomitant to MAN1B1 gene mutation are reported for the first time. More extensive case series are needed to understand whether these findings are part of the syndrome or incidental comorbid conditions.

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