Kızıltepe Meslek Yüksekokulu
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Article Citation - WoS: 11Citation - Scopus: 20Activity of Metalaxyl-M plus mancozeb, Fosetyl-Al, and Phosphorous Acid against Phytophthora Crown and Root Rot of Apricot and Cherry Caused by Phytophthora palmivora(CZECH ACADEMY AGRICULTURAL SCIENCES, 2017) Türkölmez, Şahimerdan; Derviş, SibelCrown and root rot, caused by the Oomycete pathogen Phytophthora palmivora, has become a destructive disease of apricot and cherry in eastern Turkey. There are no currently registered fungicides labeled for its control. In greenhouse experiments conducted in 2012 and 2013, 1-year-old potted apricot rootstock Zerdali and cherry rootstock Mahaleb plants were treated either with foliar spray of fosetyl-Al (140, 160, and 180 g a.i./100 l) or phosphorous acid (187.5, 200, and 215 g a.i./100 l) or soil drench of 100 ml of metalaxyl-M (= mefenoxam)+mancozeb (12+192, 16+256, and 20+320 g a.i./100 l) one day after wound inoculation of crowns and roots. In both years, phosphorous acid at 200 and 215 g a.i./100 l, fosetyl-Al at 160 and 180 g a.i./100 l, and metalaxyl-M+mancozeb at 20+320 g a.i./100 l significantly reduced the root rot severity on Zerdali by 70.68-80.00% and crown rot severity on both Zerdali and Mahaleb, by 68.32-91.96 and 74.21-82.60%, respectively, compared with phosphorous acid at 187.5 g a.i./100 l, fosetyl-Al at 140 g a.i./100 l, metalaxyl-M+mancozeb at 12+192 and 16+256 g a.i./100 l and control. Moreover, fosetyl-Al at 180 g a.i./100 l and metalaxyl-M+mancozeb at 20+320 g a.i./100 l significantly reduced the root rot severity on Mahaleb compared to fosetyl-Al at 140 and 160 g a.i./100 l, metalaxyl-M+mancozeb at 12+192 and 16+256 g a.i./100 l, phosphorous acid treatments and control in 2012, providing the best control of the disease by 88.00-90.68%. Two/three phosphorous acid foliar applications at 200 g a.i./100 l suppressed symptom development when field applications were made on a curative basis in 2014 and 2015.Article Citation - Scopus: 1Activity of nanosized copper-boron alloys against Phytophthora species(Springer Science and Business Media Deutschland GmbH, 2023) Yiğit, Uğur; Türkkan, Muharrem; İlhan, Hasan; Şimşek, Tuncay; Güler, Ömer; Derviş, SibelThis study aimed to evaluate the antifungal activity of copper-boron (Cu-B) nanoalloys against a range of Phytophthora species, including P. capsici, P. citrophthora, P. palmivora, P. cinnamomi, P. nicotianae, P. cactorum, P. plurivora, P. inundata, and P. megasperma. The nanoalloys were synthesized via mechanical alloying under an argon atmosphere, resulting in the formation of nanocrystalline Cu-B nanoalloys with irregular morphology and particle sizes ranging from 50 to 240 nm. At a concentration of 250 µg mL−1, the Cu-B nanoalloys demonstrated complete inhibition of mycelial growth, sporangium production, and zoospore germination in all tested Phytophthora species. The EC50 values for mycelial growth ranged from 28.02 to 120.17 µg mL−1, while for sporangium production and zoospore germination, they were below 10 µg mL−1. Furthermore, the nanoalloys exhibited fungicidal activity against specific Phytophthora species, such as P. capsici, P. citrophthora, P. inundata, and P. megasperma, at concentrations of 100, 250, 250, and 250 µg mL−1, respectively. Notably, the Cu-B nanoalloys displayed significant protective and curative effects on tuber rot severity in P. nicotianae-inoculated potatoes, resulting in reductions of 94.13% and 92.61% compared to the control, respectively, at a concentration of 10 µg mL−1 (P < 0.05). These findings highlight the potential of Cu-B nanoalloys as a promising fungicide for the management of plant diseases caused by Phytophthora spp.Article Citation - WoS: 9Citation - Scopus: 11Allelic variations of glutenin subunits and their association with quality traits in bread wheat genotypes(2017) Aktaş, Hüsnü; Baloch, Faheem ShehzadThe present study was conducted to evaluate the genotype × environment interaction of the yield and quality traits for five bread wheat varieties commonly grown in the Southeastern Anatolia Region of Turkey and 20 advanced lines developed within the framework of the International Winter Wheat Improvement Project. We also determined the allelic pattern of the Glu-1 and Glu-3 loci of these genotypes and examined whether these loci had an effect on the quality traits. There was a significant variation among the genotypes and environments in terms of grain yield, protein content, sedimentation volume (SV), and the extensograph dough energy value (EDEV). The results of the study indicated that genotypic effect was more influential on SV and EDEV than environmental effect; thus, both traits could be used in breeding programs to develop elite cultivars with better quality. Twelve different high-molecularweight (HMW) glutenin alleles were identified at the Glu-A1, Glu-B1, and Glu-D1 loci, resulting in 14 allelic combinations, and 17 different alleles were observed in 19 combinations for low-molecular-weight (LMW) subunits. Furthermore, among all the genotypes, 13 + 16 and 13 + 19 alleles at Glu-B1 and 5 + 12 at Glu-D1 were observed to have the lowest frequency. Our study indicated that the combinations of HMW glutenin alleles with 2* at Glu-A1, 17 + 18 and 13 + 16 at Glu-B1, and 5 + 10 at Glu-D1, as well as the combinations of LMW alleles with subunits c and d at Glu-A3; subunits d, b, c, and g at Glu-B3; and subunits a and b at Glu-D3 had positive effects on the quality traits.Article Allelic variations of glutenin subunits and their association with quality traits in bread wheat genotypes(Turkish Journal of Agriculture and Forestry, 2017) Aktaş , Hüsnü; Baloch, Faheem ShehzadThe present study was conducted to evaluate the genotype × environment interaction of the yield and quality traits for five bread wheat varieties commonly grown in the Southeastern Anatolia Region of Turkey and 20 advanced lines developed within the framework of the International Winter Wheat Improvement Project. We also determined the allelic pattern of the Glu-1 and Glu-3 loci of these genotypes and examined whether these loci had an effect on the quality traits. There was a significant variation among the genotypes and environments in terms of grain yield, protein content, sedimentation volume (SV), and the extensograph dough energy value (EDEV). The results of the study indicated that genotypic effect was more influential on SV and EDEV than environmental effect; thus, both traits could be used in breeding programs to develop elite cultivars with better quality. Twelve different high-molecularweight (HMW) glutenin alleles were identified at the Glu-A1, Glu-B1, and Glu-D1 loci, resulting in 14 allelic combinations, and 17 different alleles were observed in 19 combinations for low-molecular-weight (LMW) subunits. Furthermore, among all the genotypes, 13 + 16 and 13 + 19 alleles at Glu-B1 and 5 + 12 at Glu-D1 were observed to have the lowest frequency. Our study indicated that the combinations of HMW glutenin alleles with 2* at Glu-A1, 17 + 18 and 13 + 16 at Glu-B1, and 5 + 10 at Glu-D1, as well as the combinations of LMW alleles with subunits c and d at Glu-A3; subunits d, b, c, and g at Glu-B3; and subunits a and b at Glu-D3 had positive effects on the quality traits.Article Assessment of Parametric and Non-parametric Methods for Selecting Stable and Adapted Durum Wheat Genotypes in Multi-Environments(Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2010) Kılıç, Hasan; Akçura, Mevlüt; Aktaş , HüsnüSeventeen parametric and non-parametric methods for grain yield of 5 cultivars and 20 advanced durum wheat genotypes evaluated across 10 environments during the 2004-2007 growing seasons were used to assess performance stability and adaptability of the genotypes as well as to study interrelationship among these methods. Biplot analysis based on the rank correlation matrix indicated that most non-parametric methods were significantly inter-correlated with parametric methods. The results also showed that stability methods could be classified into four groups based on biplot analyses. The group related to the dynamic stability concept and strongly correlated with mean grain yield included the parameters of regression coefficient (bi), alpha (αi), TOP (proportion of environments in which a genotype ranked in the top third), environmental variance (Si2), coefficient of variation (CVi), Di2, Si(3) and Si(6). The second group included Wricke’s ecovalence (Wi2), the Huehn’s parameters [Si(1) Si(2)], Shukla’s stability variance (σ2i), Plaisted and Peterson’s parameter (P59) and Tai’s model (λi) which were influenced by both yield and stability simultaneously. The third group included Kang’s parameter (RS) and superiority index (Pi), which only measures stability. Genotypes 18, 16 and 2 were most stables based on parametric and non-parametric stability methods used.Article Effects of different nitrogen doses on thymoquinone and fatty acid composition in seed oil of black cumin (Nigella sativa L.)(AOCS AND WILEY, 2021) İzgi, Mehmet NecatBlack cumin,Nigella sativaL. belonging to Ranunculaceae, is a valuablemedicinal plant because of the curative effects of seed and oil. Despite studieson black cumin, there are few studies on the effects of nitrogen on fatty acidcomposition, no records on thymoquinone yield, and the thymoquinone contentof the seed oil. In this study, the effects of different nitrogen doses on the seedoil yield, thymoquinone ratio/yield, and fatty acid compositions were investi-gated. Field studies with nitrogen doses (0, 40, 60, 80, and 100 kg/ha) werestudied using the randomized plot design. As a result of the research, nitrogendoses significantly affected the oil and thymoquinone yields while insignificanton the thymoquinone ratio. The highest oil yield with 501.6 kg/ha was obtainedfrom 80 kg/ha of the nitrogen doses, while the highest thymoquinone yield with10.24 kg/ha was obtained from the control plots. Thymoquinone yields were inthe same statistical group with other nitrogen doses, except for means of100 kg/ha nitrogen doses. Linoleic, oleic, and palmitic acids were major fattyacids of black cumin, and their variations were insignificant in nitrogen applica-tions. The values of butyric, capric, and linolenic acids varied significantly in dif-ferent nitrogen doses. As a result of the study, it was determined that thehighest oil yield was obtained from 80 kg/ha nitrogen application. It has beendetermined that nitrogen doses above 60–80 kg/ha should be avoided for theseed oil and thymoquinone yields in black cumin.Article Citation - WoS: 9Citation - Scopus: 9Effects of different nitrogen doses on thymoquinone and fatty acid composition in seed oil of black cumin (Nigella sativa L.)(JAOCS, Journal of the American Oil Chemists' Society, 2021) Telci, İsa; Izgi, Mehmet Necat; Ozek, Temel; Yasak, Selma; Yur, Suleyman; Ozek, GulmiraBlack cumin, Nigella sativa L. belonging to Ranunculaceae, is a valuable medicinal plant because of the curative effects of seed and oil. Despite studies on black cumin, there are few studies on the effects of nitrogen on fatty acid composition, no records on thymoquinone yield, and the thymoquinone content of the seed oil. In this study, the effects of different nitrogen doses on the seed oil yield, thymoquinone ratio/yield, and fatty acid compositions were investigated. Field studies with nitrogen doses (0, 40, 60, 80, and 100 kg/ha) were studied using the randomized plot design. As a result of the research, nitrogen doses significantly affected the oil and thymoquinone yields while insignificant on the thymoquinone ratio. The highest oil yield with 501.6 kg/ha was obtained from 80 kg/ha of the nitrogen doses, while the highest thymoquinone yield with 10.24 kg/ha was obtained from the control plots. Thymoquinone yields were in the same statistical group with other nitrogen doses, except for means of 100 kg/ha nitrogen doses. Linoleic, oleic, and palmitic acids were major fatty acids of black cumin, and their variations were insignificant in nitrogen applications. The values of butyric, capric, and linolenic acids varied significantly in different nitrogen doses. As a result of the study, it was determined that the highest oil yield was obtained from 80 kg/ha nitrogen application. It has been determined that nitrogen doses above 60-80 kg/ha should be avoided for the seed oil and thymoquinone yields in black cumin.Article Citation - WoS: 22Citation - Scopus: 26Enhancing Drought Tolerance in Camelina Sativa L. and Canola (Brassica Napus L.) Through Application of Selenium(Pakistan Botanical Soc, 2020) Ahmad, Zahoor; Waraich, Ejaz Ahmad; Barutcular, Celaleddin; Alharby, Hesham; Bamagoos, Atif; Kizilgeci, Ferhat; El Sabagh, AymanConsidering the burning issue the present study was undertaken in pot culture at the Cholistan Institute of Desert Studies (CIDS), The Islamia University of Bahawalpur, Pakistan for enhancing drought tolerance in two oilseed crops (OC) crops camelina (Camelina sativa L.) and canola (Canola napus L.) through improving physiological, biochemical, and antioxidants activity by foliar application of selenium (Se) under drought stress. Two Camelina (i.e., 'Australian Camelina' and 'Canadian Camelina') and canola genotypes (i.e., `AARI Canola' and `UAF Canola') were used as plant materials during the growing season of 2016. Both Camelina and Canola genotypes were grown under normal (100% FC) and water deficit (drought stress) (40% FC) conditions. Four levels of Se: such as seeds priming with 75 mu M Se, foliar application of Se at 7.06 mu M and foliar Se in combination with seeds priming (7.06 mu M & 75 mu M) along with control were applied at the vegetative stage of both OC crops for screening drought tolerant genotypes. All treatments were arranged three times in a randomized complete block design. Both OC crops were grown upto the maturity and data on physiochemical, antioxidants and yield components were recorded during this study. Results of the present study indicated that the physio-biochemical parameters such as WP (water potential), OP (osmotic potential), TP (turgor pressure), proline, TSS (total soluble sugar), TFAA (total free amino acids), TPr (total proteins) and TS (total sugars); and total chlorophyll contents were improved by foliar application Se along with seed priming by Se in both OC crops in both drought stress and non -stress (control) conditions. Similarly, osmoprotectants such as GB (Glycinebetaine), anthocyanin, TPC (total phenolic contents) and flavonoids; as well as antioxidants such as APX (ascorbate peroxidase), SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) were also showed better enhancement in both OC crops through foliar application in combination with seed priming with Se (7.06 mu M & 75 mu M) under normal as well as water deficit (drought) conditions. Yield and its components i.e., branches plant(-1) (no.), 1000 -seed weight (g), seed and biological yield (t ha(-1)) of both OC crops were increased through foliar application in combination with seed priming by Se (7.06 mu M & 75 mu M) under drought and non-drought stress conditions. Both camelina and Canola genotypes categorized based on all the above-mentioned parameters under the water deficit (drought stress) condition and foliar application of Se, the genotype 'Canadian Camelina' maintained the highest values for all these attributes. Therefore, it is revealed that foliar application in combination with seed priming by Se helps to improve drought tolerance of OC crops and also leads to an increase in the productivity of crops under drought stress. Among the genotypes, 'Canadian Camelina' performed the best when seeds of the genotypes were primed with Se in combination with foliar application of Se at the vegetative stage.Article Citation - WoS: 1Citation - Scopus: 2First report of fruit rot of eggplant caused by Pythium viniferum in Turkey(SpringerLink, 2021) Türkölmez, Şahimerdan; Özer, Göksel; Çiftçi, Osman; Derviş, SibelIn August 2019, symptoms including dark brown and irregular sunken lesions or blights on the fruit pedicel and calyx of eggplants (Solanum melongena L.) occurred with a 3% incidence in two felds in Şanlıurfa province of Turkey.annotation.listelement.badge Citation - WoS: 1Citation - Scopus: 1First report of Fusarium oxysporum causing wilt on lavender (Lavandula angustifolia) in Turkey((Journal of Plant Pathology, 2021) Güney, İnci Güler; Özer, Göksel; Koşar, İslim; Derviş, Sibel; Günen, Utku[No abstract available]Article Citation - WoS: 5Citation - Scopus: 5First report of Lasiodiplodia theobromae causing dieback on almond (Prunus dulcis) in Turkey(Springer, 2021) Özer, Göksel; Türkölmez, Şahimerdan; Derviş, SibelTurkey is the world's fourth-largest almond [Prunus dulcis (Mill.) D.A. Webb] producer. In July 2020, 10% of 2,600 9-year-old almond trees cultivars Ferragnes and Ferraduel in Bozova district of Şanlıurfa province showed symptoms of yellowing, gumming, branch and trunk cankers, and dieback. Surface-disinfected wood tissue samples exhibiting visible internal necrosis were placed on potato dextrose agar (PDA). After seven days of incubation at 25 °C in the dark, 12 similar fungal colonies with dark grey colouration were isolated. The isolates Lt01Pd and Lt02Pd were randomly selected for identification, pathogenicity, and deposited in Bolu Abant Izzet Baysal University Culture Collection with accession number BAIBU0738-0739. Fruiting bodies were stromatic, black, 150–250 µm in diameter, and irregular or globose pycnidia with an apical ostiole. Paraphyses produced within pycnidia were hyaline, cylindrical, septate, and up to 60 µm long. Immature conidia formed inside the pycnidia were initially unicellular, hyaline, and ellipsoidal, whilst mature conidia were one-septate, dark brown, and measuring 18.5–23.0 μm × 11.5–13.5 μm (n = 50). BLAST searches for the internal transcribed spacer of rDNA region (ITS: GenBank accession Nos. MW733864-MW733865), translation elongation factor 1-α gene (TEF-1α: GenBank accession Nos. MW733862-MW733863), and β-tubulin 2 gene (BT2: GenBank accession Nos. MW733860-MW733861) sequences at GenBank exhibited 99.81% similarity for ITS (NR_111174) and 100% similarity for TEF-1α (AY640258) and BT2 (EU673110) sequences of type strain CBS 164.96 of Lasiodiplodia theobromae (Pat.) Griffon & Maubl. Pathogenicity of each isolate on ten 1-year-old P. dulcis cv. Ferragnes seedlings was verified by replacing five-mm diameter bark disks of stems with the same-sized mycelial plugs. Control seedlings (n = 10) were inoculated with sterile PDA plugs. All plants were maintained in a greenhouse at 25 ± 2 °C. In the inoculated plants, necrotic lesions with an average length of 6 to 8 cm on wood tissues were observed within 4 weeks. Control seedlings remained symptomless. The same fungus was only re-isolated from symptomatic tissues. In California, a similar canker disease of almond caused by L. theobromae has been reported (Chen et al. 2013). To our knowledge, this is the first report of L. theobromae causing dieback on almond in Turkey (Farr and Rossman 2021).Article Citation - WoS: 5Citation - Scopus: 6First report of leaf blight of Turkish oregano (Origanum onites) caused by Neoscytalidium dimidiatum in Turkey(SpringerLink, 2021) Alkan, Mehtap; Özer, Göksel; Koşar, İslim; Güney, İnci Güler; Derviş, SibelIn July 2020, a leaf blight disease with intense defoliation on Turkish oregano (Origanum onites L.) plants was observed in a pilot experiment feld in Akçakale and a commercial feld in Haliliye with an incidence of 5.4 and 11.7%, respectively, in Şanlıurfa province. After a surface-sterilization for 1 min with 1% NaOCl, isolations from symptomatic tissues of six afected plants for each feld consistently yielded a fungus with the same colony morphology on potato dextrose agar. Cultures were powdery with a thick aerial mycelium, initially white that changed from olive green to black within 10 days.Article Citation - WoS: 4Citation - Scopus: 4First report of Macrophomina phaseolina causing charcoal rot on common sage (Salvia officinalis) in Turkey(SpringerLink, 2021) İslim, Koşar; Güney, İnci Güler; Derviş, Sibel; Kırlı, Onur; Özer, GökselCommon sage (Salvia officinalis L.) is a perennial herb or sub-shrub native to the eastern Mediterranean. In July 2020, symptoms of chlorosis, wilting, and root rot appeared in 25% of two-year-old S. officinalis cv. Elif plants in two fields (N 36°53'42.457'', E 38°55'34.777''; N 36°53'27.236'', E 38°55'38.618'') in Şanlıurfa, Turkey.Article Citation - WoS: 3Citation - Scopus: 3First report of Macrophomina phaseolina causing charcoal rot on lemon balm (Melissa officinalis) in Turkey(SpringerLink, 2022) Koşar, İslim; Güney, İnci Güler; Üner, Senem Ece; Özer, Göksel; Derviş, SibelLemon balm (Melissa ofcinalis L.) is native to the Eastern Mediterranean region and Western Asia and is a well-known medicinal plant of Lamiaceae due to its valuable aqueous and alcoholic extracts (De Sousa et al. 2004). In the summer of 2021, wilting and root rot were observed on about 20% of two-year-old M. ofcinalis cv. Melis plants in a feld of Koruklu village, Akçatepe district of Şanlıurfa, Turkey.Article Citation - WoS: 2Citation - Scopus: 2First report of needle blight of blue spruce (Picea pungens) caused by Neoscytalidium dimidiatum in Turkey(SpringerLink, 2023) Derviş, Sibel; Türkölmez, Şahimerdan; Güney, İnci Güler; Alkan, Mehtap; Özer, GökselThe blue spruce (Picea pungens Engelm.) is an exotic conifer species widely used for decorative purposes in landscaping and private gardens. In 2020, 60% of forty blue spruce trees between 20 and 25 years old planted in front of buildings in Kavaklıdere, Ankara, displayed needle blight symptoms. Needles on the lower and interior crown closest to the trunk were infected from the bottoms of branches. They showed a brown-colored or burned appearance before dropping off, starting from their tips, resulting in up to a 65% reduction of the canopy. On the surface of infected tissues, a black-colored fungal mass was observed. Fungal colonies isolated from twenty symptomatic branches and needles were initially dark gray and became black within 4 to 7 days. Zero- to one-septate dark brown conidia (4.6 to 9.8 × 3.6 to 7.5 μm) formed in arthric chains were disarticulating and cylindrical-truncate to rod-shaped, oblong, ellipsoidal, doliiform, or globose, consistent with the description of Neoscytalidium spp. (Crous et al. 2006). The internal transcribed spacer, translation elongation factor 1-α, and β-tubulin loci of a randomly selected isolate (Nd_Pp01) were sequenced using the primers ITS1/4, EF1-728 F/986R, and Bt2a/Bt2b and deposited in GenBank under the accession numbers OK643641, OK666381, and OK666382, respectively. The sequences had 99.77–100% nucleotide identity with those of the type specimen of N. dimidiatum, CBS 145.78. Phylogenetic analysis with concatenated sequences further confirmed the identification. The isolate was deposited in the Bolu Abant Izzet Baysal University Culture Collection with accession number BAIBU-179. A pathogenicity test was conducted with ten 1-year-old P. pungens plants by spraying a conidial suspension (105 conidia/ml) of the isolate Nd_Pp01 onto the needles (Türkölmez et al. 2019). Five seedlings sprayed with sterile water were used as controls. All plants were placed in a growth chamber at 28°C with 70% RH. Spray-inoculated needles initially showed yellow spots within two weeks and turned brown six weeks after inoculation, whereas the controls remained symptom-free. The pathogen was re-isolated from the inoculated needles but not from controls. To our knowledge, this is the first report of N. dimidiatum causing needle blight on P. pungens in Turkey and worldwide (Farr and Rossman 2023).Article Citation - WoS: 5Citation - Scopus: 7First report of Neoscytalidium dimidiatum causing foliar and stem blight of lavender in Turkey(SpringerLink, 2021) Güney, İnci Güler; Özer, Göksel; Turan, İrem; Koşar, İslim; Derviş, SibelLavender (Lavandula angustifolia Mill.) is a valuable medicinal and aromatic plant in Turkey, with a cultivated area of 2,218 hectares in 2020 (TURKSTAT 2021). In June 2020, wilting, extensive stem and leaf blight or necrosis were observed in two-year-old lavender plants cultivated in the experimental felds of the GAP Agricultural Research Institute, Şanlıurfa, Turkey.Article First report of Verticillium dahliae causing Verticillium wilt on avocado (Persea americana) in Turkey(SpringerLink, 2022) Tok, Fatih Mehmet; Derviş, SibelAvocado (Persea americana Mill.) is an expanding crop in Turkey grown on 949 ha, yielding 4,209 tons of fruit annually (FAO 2021). In June 2021, 5% of 220 5-year-old trees of cv. Hass grafted onto the rootstock Fuerte (36°06′25.6"N 35°59′16.7"E) in Samandağ district of Hatay showed symptoms of twig and branch dieback with brownish black leaf and bark discoloration. Vascular tissues of branches bearing blackened dead twigs were sampled from five trees. Tissues were surface disinfected in 1% NaOCl for 2 min, rinsed with sterile distilled water (SDW), dried, placed on potato dextrose agar, and incubated at 25 °C. After 7 days, a slow-growing fungus was consistently recovered. Colonies were identified as Verticillium dahliae Kleb. on the basis of the presence of black microsclerotia (35.2 to 160.8 × 20.3 to 68.5 μm), verticillate conidiophores, and hyaline, elliptical, single celled conidia (2.9 to 7.3 × 2.2 to 3.8 μm) (Pegg and Brady 2002). Identification was confirmed by sequencing the internal transcribed spacer region (ITS) of rDNA amplified from one single-conidial isolate (AvVd01 deposited in the plant pathogenic fungal collection of the first author’s institution) using universal ITS1/ITS4 primers. BLAST analysis of the amplicon sequenced (GenBank accession No. MZ664289) showed 100% identity with a sequence of V. dahliae from kiwifruit in Turkey (MK287620.1). To fulfill Koch's postulates, 15 healthy 1-year-old P. americana ‘Hass’ seedlings were inoculated by submerging trimmed roots in a conidial suspension of 107 conidia/ml for 5 min using AvVd01. Ten control plants were dipped in SDW in the same manner. Inoculated plants showed symptoms identical to those of naturally infected plants within a month. V. dahliae was reisolated from inoculated plants but not from controls. To our knowledge, this is the first report of V. dahliae causing wilt on avocado in Turkey (Farr and Rossman 2022). It is expected to cause more problems in avocado plantings previously dedicated to vegetable crops.Article Citation - WoS: 73Citation - Scopus: 84Normalized Difference Vegetation Index and Chlorophyll Content for Precision Nitrogen Management in Durum Wheat Cultivars under Semi-Arid Conditions(Sustainability (Switzerland), 2021) Kızılgeçi, Ferhat; Yıldırım, Mehmet; İslam, Mohammad Sohidul; Ratnasekera, Disna; Iqbal, Muhammad Aamir; Sabagh, El AymanTo impart sustainability to modern intensive farming systems, environmental pollution caused by nitrogenous fertilizers needs to be reduced by optimizing their doses. To estimate the grain yield and nutrtional quallity of wheat, the normalized difference vegetation index (NDVI) and chlorophyll content (SPAD) are potential screening tools to identify the N deficiency and screen out the promising cultivars. The two-year field study was comprised of five levels of nitrogen (N) (control, 50, 100, 150 and 200 kg N ha-1) and two durum wheat genotypes (Sena and Svevo). The experimental design was split-plot, in which N levels were placed in the main plots, while wheat genotypes were arranged in sub-plots. To predict the yield and quality traits, NDVI and SPAD values recorded at heading, anthesis and milky growth stages were taken as response variables. The results revealed that N fertilization significantly influenced SPAD and NDVI attributed traits of durum wheat, except NDVI at milky stage (NDVI-M) during the first year. The maximum value of NDVI was recorded by 150 kg N ha-1, while control treatment gave the minimum value. The grain yield was increased with the increasing dose of N up to 100 kg N ha-1 (4121 kg ha-1), and thereafter, it was declined with further increased N levels. However, the variation between genotypes was not significant, except NDVI and SPAD values at the milky stage. The genotype Svevo had the highest NDVI values at all growth stages, while the genotype Sena recorded the maximum SPAD values during both years. Similarly, N levels significantly influenced the quality traits (protein, wet gluten, starch test weight and Zeleny sedimentation) of both genotypes. The highly significant relationship of SPAD and NDVI with the grain yield and yield attributes showed their reliability as indicators for determining N deficiency and selection of superior wheat genotypes for ensuring food security under climate change scenario.Article Citation - WoS: 8Citation - Scopus: 11Phenology, Growth and Yield Are Strongly Influenced by Heat Stress in Late Sown Mustard (Brassica Spp.) Varieties(Pakistan Botanical Soc, 2020) Bazzaz, Md Mahfuz; Hossain, Akbar; Farooq, Muhammad; Alharby, Hesham; Bamagoos, Atif; Nuruzzaman, Md; El Sabagh, AymanIn rice-based cropping system of northern Bangladesh, mustard planting is delayed due to late harvest of monsoon rice (Transplanted Aman rice; T. Aman rice). The late sown mustard is often exposed to high-temperature stress during the reproductive stage, which causes a significant decrease in seed yield. Most of the farmers plant a low yielding local mustard variety 'Tori-7', which is sensitive to high-temperature stress. The Oil Seeds Research Centre of Bangladesh Agricultural Research Institute has recently released short duration and high-yielding mustard varieties for the rice-based cropping system of northern Bangladesh to tackle this issue. However, the performance of these varieties in the target cropping system under late sown conditions (together with exposure to heat stress) has not been evaluated yet. Therefore, this two-year field experiment was conducted to assess the performance of newly released mustard varieties 'BARI Sarisha-14' and `BARI Sarisha-15' in comparison with the local check variety 'Tori-7' sown at five planting dates (viz. October 21, November 1, November 10, November 20, and November 30). The delay in sowing shortened time (days) to flowering and maturity, regardless of the variety. Similarly, plant height, branches/plant and total dry matter accumulation of the tested varieties were also reduced upon delay in planting. The greatest seed yield was observed in varieties `BARI Sarisha-15' and `BARI Sarisha-14' when sown on October 21. These two varieties produced fair seed yield when sown at November 20. However, the local variety 'Tori7' yielded the least across all the planting dates. Therefore, the varieties `BARI Sarisha-14' and `BARI Sarisha-15' may be planted in the rice-based cropping system of northern Bangladesh after harvest of T. Aman rice to get maximum seed yield.Article Citation - WoS: 18Citation - Scopus: 20Traditional Uses of Medicinal Plants in Artuklu, Turkey(Human Ecology, 2020) Mungan Kılıç, Fatma; Kılıç, Murat; Yıldız, KemalWe conducted an ethnobotanical field study of traditional medicinal plants in 91 villages of Artuklu District among four ethnic groups: Turkish, Kurdish, Arabic, and Assyrian. We interviewed 365 elderly informants and recorded traditional uses of 85 identified botanical taxa, including four endemic species previously unidentified as medicinal plants: Arum rupicola Boiss., Gundelia mesopotamica Fırat, Alkanna trichophila Hub.-Mor., and Crocus leichtlinii (Dewar) Bowles. We gathered data specifically on medicinal uses of these taxa to determine the relative importance of the species surveyed and calculate the informant consensus factor (FIC) in relation to medicinal plant use. The highest use values were recorded for the species Teucrium polium L. (0.78), Urtica dioica L. (0.60) and Crataegus azarolus L., (0.59), while the highest FIC was for gynecological diseases (0.93).
