Bitkisel ve Hayvansal Üretim Bölümü

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Citation - WoS: 13
Citation - Scopus: 13
Molecular phylogeny of plant pathogenic fungi based on start codon targeted (SCoT) polymorphism
(Springer, 2023) Palacıoğlu, Gülsüm; Alkan, Mehtap; Derviş, Sibel; Bayraktar, Harun; Özer, Göksel
Background: A number of molecular marker systems have been developed to assess genetic diversity, carry out phylogenetic analysis, and diagnose and discriminate plant pathogenic fungi. The start codon targeted (SCoT) markers system is a novel approach used here to investigate intra and interspecific polymorphisms of phytopathogenic fungi. Materials and methods: This study assessed genetic variability between and within 96 isolates of ten fungal species associated with a variety of plant species using 36 SCoT primers. Results: The six primers generated 331 distinct and reproducible banding patterns, of which 322 were polymorphic (97.28%), resulting in 53.67 polymorphic bands per primer. All primers produced informative amplification profiles that distinguished all fungal species. With a resolving power of 10.65, SCoT primer 12 showed the highest polymorphism among species, followed by primer 33 and primer 29. Polymorphic loci (PPL), Nei's diversity index (h), and Shannon index (I) percentages were 6.25, 0.018, and 0.028, respectively. UPGMA analysis separated all isolates based on morphological classification and revealed significant genetic variation among fungal isolates at the intraspecific level. PCoA analysis strongly supported fungal species discrimination and genetic variation. The other parameters of evaluation proved that SCoT markers are at least as effective as other DNA markers. Conclusions: SCoT markers were effective in identifying plant pathogenic fungi and were a powerful tool for estimating genetic variation and population structure of different fungi species.
Effects of Humic Acid and Phosphorus Applications on Nutrient Composition of Lentil (Lens Culinaris Medic.)
(Scibulcom Ltd., 2016) Togay, Y.; Dogan, Y.; Togay, N.; Doğan, Yusuf; 06.02. Department of Plant Protection / Bitki Koruma Bölümü; 06. Faculty of Agricultural Sciences and Technologies in Kızıltepe / Kızıltepe Tarım Bilimleri ve Teknolojileri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Lentil (Lens culinaris Medic.) is an important food crop with high protein content. This study aimed to determine the effects of increasing doses of phosphorus and humic acid applications on P, K, Ca, Mg, Fe, Cu, Mn, Zn, protein ratio and seed yield in lentil cultivar. The study was carried out in the research and application during the 2008–2009 and 2009–2010 cropping seasons in field of Agriculture Faculty Yuzuncu Yil University, Turkey. The experiment comprised a factorial randomised complete block design replicated three times with three phosphorus treatments 0, 40 and 80 kg ha–1, as triple superphosphate, and three humic acid treatments 0, 300 and 600 kg ha–1. For basic fertilisation 40 kg ha–1 ammonium sulphate were applied for nitrogen. The nutrient contents of seed and yield were significantly increased by increasing phosphorus and humic acid levels except for Zn content nutrient. The combination between phosphorus at 80 kg P2O5 ha–1 and humic acid at 600 kg ha–1 gave the highest growth and production as well as minerals composition (P, K, Ca, Mg, Fe, Cu and Mn) and protein content compared to other treatments. Humic acid increased the availability of phosphors, macronutrients and micronutrients. © 2016, Scibulcom Ltd. All rights reserved.
Citation - WoS: 19
Citation - Scopus: 21
Canker and leaf scorch on olive (Olea europaea L.) caused by Neoscytalidium dimidiatum in Turkey
(ScienceDirect, 2022) Güney, İnci Güler; Özer, Göksel; Türkölmez, Şahimerdan; Derviş, Sibel
In a recent survey of olive groves in the Southeast Anatolia Region of Turkey, a new and serious decline of olive trees, beginning with foliar scorching and then dieback of twigs, branches, and even whole trees, was observed for the first time. In more advanced stages of the disease, necrosis and cankers were observed on the bark of the trunk, branches, and twigs. Isolations from symptomatic tissues from multiple cultivars in diverse locations yielded Neoscytalidium dimidiatum, which were identified using ITS, tef1, and tub2 loci of genomic DNA, in combination with morphological data. In vitro studies showed that conidial germination, hyphal growth, and pycnidia formation of the pathogen were positively correlated with elevated temperatures. Wild type olive “Delice”, “Edincik Su”, and “Memecik” seemed like the most resistant cultivars on which disease severity values were the lowest among 14 screened olive cultivars in two experimental orchards under natural conditions. Pathogenicity tests showed that “Gemlik” was the most susceptible cultivar showing the largest cankers and extensive scorch lesions. Isolates caused canker but not leaf scorch on O. europaea cultivars “Arbequina” “Halhalı”, “Manzanilla”, “Nizip Yağlık”, and “Saurani”. Neoscytalidium isolates are likely to have a negative impact on the health of diverse olive groves, which are primarily confined to Mediterranean-type climatic regions. These findings suggest an increased risk of infection in environments with increasing temperatures, as is common in the Southeast Anatolia Region of Turkey. The information gathered in this study will be used to examine the disease's epidemiology and establish disease control initiatives. This is also the first report of N. dimidiatum infecting O. europaea in the world.
Citation - WoS: 3
Citation - Scopus: 2
Nutrient Status and Yield of Chickpea (Cicer Arietinum L.) as Influence by Application of Different Harvest Timing and Sulphur Fertiliser
(Scibulcom Ltd., 2015) Dogan, Y.; Togay, N.; Togay, Y.; Doğan, Yusuf; 06.02. Department of Plant Protection / Bitki Koruma Bölümü; 06. Faculty of Agricultural Sciences and Technologies in Kızıltepe / Kızıltepe Tarım Bilimleri ve Teknolojileri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The objectives of this research were to study the effect of harvesting time and sulphur levels on the yield, protein and mineral nutritional value of chickpea seeds. Three different harvesting times (early, on-time and late) and four sulphur levels (0, 20, 40 and 60 kg ha–1) were used. Yield, protein ratio and mineral nutritional value of chickpea were significantly affected by sulphur and harvest timing. The highest grain yields according to the harvest time were obtained from on-time harvest with 1527 and 1434 kg ha–1 for the first and second years, respectively. The lowest grain yields were obtained from the control plots, whereas the highest values were obtained from the plots given 60 kg ha–1 sulphur. Generally, protein, phosphorus, calcium, magnesium, copper, zinc, and manganese concentrations of the seeds in on-time harvest and 60 kg ha–1 sulphur aplication were found to be greater than in early and late harvested plants. Positive significant relationships were found between harvest time and protein (r = 0.64**), Fe (r = 0.75**) and Zn (r = 0.27*). Positive significant relationships were found between sulphur doses and grain yield (r = 0.44*), protein (r = 0.26*), P (r = 0.71**), K (r = 0.73**), Ca (r = 0.36**), Mg (r = 0.76*), Zn (r = 0.57) and S (r = 0.96*). © 2015, Scibulcom Ltd. All rights reserved.
Citation - WoS: 5
Citation - Scopus: 6
A novel blight and root rot of chickpea: A new host for Neoscytalidium dimidiatum
(ScienceDirect, 2023) Güney, İnci Güler; Bozoğlu, Tuba; Özer, Göksel; Derviş, Sibel
In the southeastern province of Mardin in Turkey, a severe and novel fungal infection affected all chickpea plant parts, resulting in blight symptoms on stem, petiole, branch, and leaf, defoliation, seed and root rot, and death. Neoscytalidium dimidiatum was identified as the agent responsible for this new blight and root rot using sequence analysis of the ITS, tef1, and tub2 loci as well as morphological data. The pathogen was found in all surveyed districts and fields, with varying incidences of blight and root rot, blight being nearly twice (40%) as common as root rot (21%), and root-rotted and blighted plants co-occurring in the majority of instances. All 92 N. dimidiatum isolates from various tissues induced necrotic lesions on the inoculated plants, indicating they were pathogenic for chickpea. Conidia exhibited germination across a temperature range, with increasing temperatures positively influencing germination rates, and mycelial growth was significantly influenced by temperature, with the optimal growth temperature observed to be 35 °C. The response of 25 Turkish chickpea cultivars and three other genotypes when subjected to inoculation with Ciar 12 and Ciar 78 isolates, representative of phylogenetic clusters, was evaluated based on the severity of blight and root rot. The majority of cultivars and genotypes displayed high susceptibility and suffered mortality when exposed to either soil or spray inoculation with each isolate. Among the assessed cultivars and genotypes, Çağatay had the lowest severity of root rot. This study is the first to report a natural infection of chickpea plants by N. dimidiatum. Under global warming, this may be detrimental to chickpea cultivation and habitat in southeastern Turkey, which is the origin of chickpeas.
Land Suitability Assessment for Pistachio Cultivation Using GIS and Multi‑Criteria Decision‑Making: A Case Study of Mardin, Turkey
(Environ Monit Assess, 2023)
Site selection for pistachio orchards is an important issue for sustainable agricultural policies, crop productivity, agricultural planning, and communities. This study aims to investigate suitable places for pistachio in the Mardin Province (SE Turkey) by considering several variables, such as meteorological data, topographic conditions, economic factors, and soil characteristics, using Geographic Information System (GIS) and Multi-Criteria Decision Analysis. Pistachio farmers, expert opinions, and literature data were used to determine the requirements for pistachio cultivation. Four main assessment criteria (thirteen sub-criteria), sixty value ranges, and fourteen exclusion criteria were determined for the pistachio land suitability assessment. The weighting of the evaluation criteria was calculated using the Analytical Hierarchy Process (AHP). Farmers and experts have stated that meteorological factors are more important than soil, topography, and economic factors. All data were transferred to the GIS environment, and a land suitability map was created using the weighted linear combination method. The results show that Mardin province has very suitable lands for pistachio cultivation. The resulting map determined that the 228,891.59 ha area in Mardin province is very suitable for pistachio. To evaluate the accuracy of the land suitability map generated for pistachio, the Receiver Operating Characteristic (ROC) curve was used. The value of the area under the curve (AUC) was calculated to be 0.806, which indicates that the study is consistent. The created suitability map will be an essential data source for developing sustainable agricultural strategies in the Southeastern Anatolia region.
Citation - WoS: 6
Citation - Scopus: 8
Lasiodiplodia theobromae and Neoscytalidium dimidiatum associated with grafted walnut (Juglans regia L.) decline in Turkey
(SpringerLink, 2023) Derviş, Sibel; Osman Çiftçi, Göksel Özer, Şahimerdan Türkölmez, Sibel Derviş
Surveys of canker diseases in 26 walnut orchards were conducted in the southeastern Turkish provinces of Batman, Diyarbakır, and Şanlıurfa in 2020. Decline symptoms on trees were most severe in the trunks of grafted trees grown on unproductive local rootstocks with Chandler and Franquette scions throughout the surveyed areas, and samples were taken from those trees. Lasiodiplodia theobromae was identifed from the cankers on the trees of the seven orchards, while Neoscytalidium dimidiatum was identifed from the other 19 orchards based on morphological characteristics. DNA sequencing analysis of 10 representative isolates using the ITS1-5.8S-ITS2 (ITS), the large subunit (LSU), and the translation elongation factor 1-alpha (EF-1α) genes confrmed the identifcation of the causal fungi. Pathogenicity tests on 2 year-old Chandler seedlings revealed that L. theobromae and N. dimidiatum produced lesion length averages of 7–8 cm and 15–25 cm after 3 weeks of stem-inoculation, respectively. Koch’s postulates were confrmed by successfully reisolating the fungi only from plants inoculated. This is the frst study to document L. theobromae as the causal agent of walnut tree decline and cankers in Turkey.
Citation - Scopus: 2
Correlation and path coefficient analysis for yield and some yield components of wheat (Triticum aestivum l.)
(Scibulcom Ltd., 2017) Togay, Necat; Togay, Yeşim; Doğan, Yusuf
The experiment was conducted to determine the relationship among yield and some yield components using correlation and path coefficient analysis. This study was performed under Mardin conditions the years of 2011–2012 and 2012–2013. Fifteen bread wheat varieties were used in the experiments. The randomised complete blocks design with 3 replications was used. The characteristics such as plant height, spike height, numbers of seed per spike, seed yield, harvest index, biological yield and 1000 seed weight were investigated in the study. Positive and statistically significant (p < 0.01) relationships were determined between seed yield, spike height, numbers of seed per spike, biological yield and harvest index. According to path coefficient analysis, there were strong direct effects of the biological yield and harvest index on the seed yield, p = 0.53549 and 0.548321, respectively. The results of this study indicate that numbers of seed per spike, biological yield and harvest index affected seed yield and it was concluded that these characters should be considered as significant selection criteria in wheat breeding for yield under the regional conditions of southeast Turkey. © 2017, Scibulcom Ltd. All rights reserved.
Citation - WoS: 17
Citation - Scopus: 19
First Report of Neoscytalidium Dimidiatum Causing Black Canker and Root Rot of Walnut in Turkey
(Amer Phytopathological Soc, 2019) Dervis, S.; Turkolmez, S.; Ciftci, O.; Serce, C. Ulubas; Dikilitas, M.
Citation - WoS: 29
Citation - Scopus: 30
The characterization of phenolic compounds via LC-ESI-MS/MS, antioxidant, enzyme inhibitory activities of Salvia absconditiflora, Salvia sclarea, and Salvia palaestina: A comparative analysis
(ScienceDirect, 2022) Önder, Alev; İzgi, Mehmet Necat; Cinar, Ahsen Sevda; Zengin, Gökhan; Yılmaz, Mustafa Abdullah
Abstract Salvia L. genus, one of the medicinal and aromatic plants belonging to the Lamiaceae family, is represented by many endemic species in temperate regions worldwide. The Salvia species have plenty of usages in traditional medicine, important pharmacological effects and economic value. In this context, this study was conducted to evaluate the chemical profile and potential bioactivity of ethyl acetate extracts from 3 different Salvia species; Salvia absconditiflora (Salvia cryptantha- an endemic species), S. sclarea and S. palaestina collected from different localities in Anatolia. Total phenolic-flavonoid contents, antioxidant properties and enzyme inhibition experiments were investigated in tested Salvia species. The phenolic profiles were also determined by liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The analysis revealed the presence of phenolic compounds in ethyl acetate extracts such as cynaroside, rosmarinic acid, cosmosiin, luteolin, apigenin and acacetin. The highest level of phenolic components was detected in S. absconditiflora with 74.16 mg GAE/g. The best radical scavenging and reducing abilities were found in S. absconditiflora (DPPH: 251.39 mg TE/g; ABTS: 340.16 mg TE/g; CUPRAC: 496.20 mg TE/g; FRAP: 322.94 mg TE/g). Moreover, the inhibition of AChE, BChE, and α-amylase has also been studied. The most potent AChE inhibitory ability was recorded in S. sclarea (4.00 mg GALAE/g), followed by S. palaestina (3.38 mg GALAE/g) and S. absconditiflora (3.01 mg GALAE/g). The extracts exhibited similar amylase inhibitory effects. These results reveal that Salvia species [S. absconditiflora (S. cryptantha), S. sclarea and S. palaestina] confirming their potential can be rich sources of promising bioactive compounds as a starting point for further analysis. In addition, it is thought that this study may be the new report for the construction of helpful databases in terms of shedding light on future research and researchers with the valuable information obtained about these economically important Salvia species.
Citation - WoS: 5
Citation - Scopus: 5
First Report of Neoscytalidium Dimidiatum Causing Dieback, Shoot Blight, and Branch Canker of Willow Trees in Turkey
(Amer Phytopathological Soc, 2019) Turkolmez, Sahimerdan; Dervis, Sibel; Ciftci, Osman; Serce, Cigdem Ulubas; Turkohmez, Cemile Gulden; Dikilitas, Murat
Citation - WoS: 9
Citation - Scopus: 10
Exploring genetic diversity and Population structure of five Aegilops species with inter-primer binding site (iPBS) markers
(SpringerLink, 2022) Kızılgeçi, Ferhat; Bayhan, Bora; Türkoğlu, Aras; Haliloğlu, Kamil; Yıldırım, Mehmet
Background: Turkey is not only a center of origin for wheat, but also contains wild forms of various cereals. Turkey, located in the Fertile Crescent, has conserved its genetic richness to the present day. The aim of the study was to investigate the genetic diversity of 70 wild wheat species, to evaluate the structure of diversity in germplasm and to generate useful data for further breeding programs. Methods and results: Genetic diversity and population structure of 70 wild wheat species (Ae. cylindrica, Ae. geniculata, Ae. triuncialis, T. dicocoides, Ae. columnaris) collected from Eastern and Southeastern Anatolia regions of Turkey were investigated in this study with the use of inter-primer binding site (iPBS) markers. Of 35 iPBS primers used, 11 yielded a total of 61 alleles. Number of alleles per marker varied between 2 (iPBS-2085) and 9 (iPBS-2394) with an average value of 5.55. Polymorphic information content (PIC) values varied between 0.22 and 0.47, with an average value of 0.35. Average number of effective alleles (Ne) was identified as 1.9488, Nei's genetic diversity (H) as 0,4861 and Shannon's information index (I) as 0.6791. Cluster analysis through unweighted pair-group mean average (UPGMA) method revealed that 70 wild wheats were divided into three main clusters. Genetic similarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 19% (YB2 and YB70) and 98% (YB66 and YB67). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 62.33% of total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2666 (the first sub-population) and 0.2330 (third sub-population), with an average value of 0.2500. Average population differentiation measurement (Fst) was identified as 0.3716 for the first sub-population, 0.3930 for the second subpopulation and 0.4804 for the third sub-population. Conclusions: Based on present findings population structure of 70 wild wheat genotypes collected from Eastern and Southeastern Anatolia regions of Turkey were successfully characterized with the use of iPBS markers. Present findings suggested that iPBS-retrotransposon markers could reliably be used to elucidate genetic diversity of Aegilops genotypes.
Citation - WoS: 5
Citation - Scopus: 7
First report of Neoscytalidium dimidiatum causing foliar and stem blight of lavender in Turkey
(SpringerLink, 2021) Güney, İnci Güler; Özer, Göksel; Turan, İrem; Koşar, İslim; Derviş, Sibel
Lavender (Lavandula angustifolia Mill.) is a valuable medicinal and aromatic plant in Turkey, with a cultivated area of 2,218 hectares in 2020 (TURKSTAT 2021). In June 2020, wilting, extensive stem and leaf blight or necrosis were observed in two-year-old lavender plants cultivated in the experimental felds of the GAP Agricultural Research Institute, Şanlıurfa, Turkey.
Citation - WoS: 4
Citation - Scopus: 6
Effect of Different Harvest Dates to Essential Oil Components of Oil-Bearing Rose (Rosa damascena Mill.) in Mardin
(Taylor & Francis Online, 2022) İzgi, Mehmet Necat
Rosa damascena Mill. is the most prominent rose source for the manufacturing of rose oil, rose water, concrete, and absolute from flower petals. The purpose of this study was to see how different harvest dates affected the essential oil components and rates obtained from flowers in oil rose planted for the first time in Turkey's Mardin Province. Blossoms were taken five times with three replications, at around one-week intervals, from a garden in the Yaylabaşı district at 6:00 a.m. (4, 11, 18, 24, and 31 May). Animal manure and leonardite were used instead of chemical fertilizers for plants. No chemical pesticides have been applied. Essential oil components of the oils obtained by a water distillation system (Clevenger apparatus) were determined by GC/MS. The citronellol ratio, which is the most important component for the quality of the essential oil in the plant, was found to be highest (35.90%) in the first harvest period and lowest in the last harvest period (17.57%). Geraniol levels decreased in the first three harvests, with the greatest level (27.16%) coming in the last harvest. The percentages of citronellol/geraniol varied between 1.51 and 0.65 depending on the different harvest dates. Nerol rates peaked at 15.44% on the first harvest, progressively declined until the fourth harvest (7.92%), then slightly increased on the fifth harvest. Nonadecane continuously increased from the first to the last harvest (4.10%-11.66%). No methyl eugenol was detected. Mardin is a promising location for oil-bearing rose cultivation, given the essential oil components and their ratios.
Citation - WoS: 4
Evolotion Barley Genotypes in Multi-Environment Trials by Ammi Model and Gge Biplot Analysis
(Parlar Scientific Publications (p S P), 2019) Oral, Erol; Kendal, Enver; Kilic, Hasan; Dogan, Yusuf; Doğan, Yusuf; Oral, Erol; Kendal, Enver; 06.02. Department of Plant Protection / Bitki Koruma Bölümü; 16.02. Department of Seed Production / Tohumculuk Teknolojisi Bölümü; 06. Faculty of Agricultural Sciences and Technologies in Kızıltepe / Kızıltepe Tarım Bilimleri ve Teknolojileri Fakültesi; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The uniformity of genotypes are significant for crop breeding program decisions to improve new varieties. The AMMI (Additive main effects and multiplicative interaction) analysis and Genotype x Environment Interaction (GEI) is make to estimation grain yield and understands GxE interaction patterns by researches as differential ranking of variety yields in multi-environment trials. Therefore, fifteen barley advanced line and six national cultivars and four foreign varieties (registered in abroad) were used in the study. The experiments were performed according to a complete randomized block design with four replications at five environments during two years. The stability and superiority of genotypes for yield and other traits were determined using AMMI and GGE biplot analysis. Factors (G, GE, and GEI) were found to be highly significant (P < 0.01) for grain yield. AMMI analysis indicated that the major contributions to treatment sum of squares were environments (98.52%), GE (0.45%) and genotypes (1.02%), respectively, suggesting that grain yield of genotypes were effected environmental conditions. The GGE biplot indicated that PCA 1 axes (Principal component) was significant as P<0.01 and supplied to 49.36% of complete GxE interaction. The AMMI indicated that G8 and G23 desirable and stabile genotypes for grain yield in multi-environment. Moreover, E2 and E5 (irrigated environments) were high yielding, while E3 (drought stress) low yielding as forecast. On the other hand, GGE biplot indicated that three group were occurred among traits, first group (GY: grain yield, CC: crude cellulose, CD: cold damage), second group (PC:, HVV: hectoliter weight, TGW: thousand grain weight, SH: seed humidity), third group (LOD: lodging, PH: plant height, HT: heading time). Moreover: the study showed that G3, G6, G7, G8, G13 and G21 were the best genotypes both grain yield and other traits. The results of AMMI model and GGE biplot indicated that G8 is suitable to recommend for release and G23 desirable origin for yield stability and G7 valuable source for quality to use in barley breeding program.
Citation - WoS: 8
Citation - Scopus: 10
Clonostachys rosea Strain ST1140: An Endophytic Plant-Growth-Promoting Fungus, and Its Potential Use in Seedbeds with Wheat-Grain Substrate
(SpringerLink, 2023) Türkölmez, Şahimerdan; Özer, Göksel; Derviş, Sibel
In this study, Clonostachys rosea strain ST1140, a naturally occurring endophyte in healthy roots of a pistachio tree, was identifed morphologically and molecularly through DNA sequencing, and its endophytic ability and growth efect in/on three solanaceous plant species were tested. Three diferent organic substrates (bread wheat-grain, sawdust, and leonardite) were also investigated for their utility in cultivating and multiplying the strain. In-tray and pot experiments, the rates of seed germination and vegetative development of pepper, tomato, and eggplant inoculated with C. rosea at planting were compared to those of non-inoculated controls. In pot experiments, inoculating seedbeds with increasing doses of C. rosea strain ST1140 with wheat-grain substrate resulted in higher plant height values for all plant species, and the strain endophytically colonized the roots of all plant species. In-tray experiments revealed that ST1140 inoculation resulted in 2–4 days earlier and 10% higher germination rates for all plant species, as well as more vigorous and accelerated seedling growth (10–13 days earlier for seedlings at the 4–5 true leaf stage) on all inoculated plant species. Among three diferent organic substrates, wheat-grain was found to be the most efective for long-term cultivation and multiplication of the fungus, which could be of interest for its development as a commercial product. These results promised the strain ST1140’s use as a biofertilizer in seedbeds with a wheat-grain substrate.
Citation - WoS: 12
Citation - Scopus: 16
First report of Neoscytalidium dimidiatum associated with dieback and canker of common fig (Ficus carica L.) in Turkey
(SpringerLink, 2022) Güney, İnci Güler; Bozoğlu, Tuğba; Özer, Göksel; Türkölmez, Şahimerdan; Derviş, Sibel
In 2020, a canker disease with dieback of branches and decline of various fig trees in Şanlıurfa and Mardin provinces of Turkey was observed. The causal pathogen was identified as Neoscytalidium dimidiatum based on morphological characteristics and phylogenetic analysis of ITS, tef1, and tub2 loci. Koch’s postulates were confirmed by successful re-isolation of N. dimidiatum only from plants inoculated with the pathogen. This is the first report of N. dimidiatum associated with dieback and canker of common fig in Turkey.
Citation - WoS: 9
Citation - Scopus: 9
Effects of different nitrogen doses on thymoquinone and fatty acid composition in seed oil of black cumin (Nigella sativa L.)
(JAOCS, Journal of the American Oil Chemists' Society, 2021) Telci, İsa; Izgi, Mehmet Necat; Ozek, Temel; Yasak, Selma; Yur, Suleyman; Ozek, Gulmira
Black cumin, Nigella sativa L. belonging to Ranunculaceae, is a valuable medicinal plant because of the curative effects of seed and oil. Despite studies on black cumin, there are few studies on the effects of nitrogen on fatty acid composition, no records on thymoquinone yield, and the thymoquinone content of the seed oil. In this study, the effects of different nitrogen doses on the seed oil yield, thymoquinone ratio/yield, and fatty acid compositions were investigated. Field studies with nitrogen doses (0, 40, 60, 80, and 100 kg/ha) were studied using the randomized plot design. As a result of the research, nitrogen doses significantly affected the oil and thymoquinone yields while insignificant on the thymoquinone ratio. The highest oil yield with 501.6 kg/ha was obtained from 80 kg/ha of the nitrogen doses, while the highest thymoquinone yield with 10.24 kg/ha was obtained from the control plots. Thymoquinone yields were in the same statistical group with other nitrogen doses, except for means of 100 kg/ha nitrogen doses. Linoleic, oleic, and palmitic acids were major fatty acids of black cumin, and their variations were insignificant in nitrogen applications. The values of butyric, capric, and linolenic acids varied significantly in different nitrogen doses. As a result of the study, it was determined that the highest oil yield was obtained from 80 kg/ha nitrogen application. It has been determined that nitrogen doses above 60-80 kg/ha should be avoided for the seed oil and thymoquinone yields in black cumin.
Citation - WoS: 15
Citation - Scopus: 21
Variation in essential oil composition of coriander (Coriandrum sativum L.) varieties cultivated in two different ecologies
(TAYLOR & FRANCIS INC, 2017) Izgi, Mehmet Necat; Telci, Isa; Elmastas, Mahfuz
Coriander, Coriandrum sativum L, is an important medicinal plant belonging to Apiaceae family, which is grown in many parts of Turkey. In this study, essential oil and composition of 6 Turkish coriander cultivars (Gamze, Arslan, Erbaa, Pelmus, Kudret, Gurbuz) were examined in two different (Mardin and Tokat) ecologies. Essential oils were isolated with distillation method in maturated fruits and component composition was determined with gas chromatography-mass spectrometry (GC-MS). Main component was linalool in all cultivar, and it was higher in the conditions of Mardin having a warmer climate in all the cultivars than that of Tokat with temperate climate. Contrary to linalool, -pinene and neryl acetate contents of all cultivar were higher in Tokat.
Citation - WoS: 23
Citation - Scopus: 24
New disease caused by Neoscytalidium dimidiatum devastates tomatoes (Solarium lycopersicum) in Turkey
(ELSEVIER SCI LTD, 2019) Turkolmez, Sahimerdan; Dervis, Sibel; Ciftci, Osman; Serce, Cigdem Ulubas; Dikilitas, Murat
A novel disease of tomato (Solarium lycopersicum L.) was observed in the Southeast Anatolia Region of Turkey. Symptoms were blight of all aerial parts of the plant, including stems, branches, leaves, petioles, flowers and fruits, defoliation, root rot, inner stem necrosis, and plant death. The disease was found in 13.9% of surveyed fields, with an incidence varying from 3% to nearly 75% (average 21.2%) of the plants in symptomatic fields. The average severity of blight on stem in fields with the symptomatic plant surveyed was 1.4%. A Botryosphaeriaceae species, identified as Neoscytalidium dimidiatum (Penz.) Crous & Slippers using morphological and cultural features, was consistently isolated from symptomatic roots, inner stems, and blighted leaves, shoots, stems, fruits and flowers. The partial nucleotide sequence data for three gene loci, including nuclear rDNA internal transcribed spacer (ITS), large subunit (LSU) genes and the translation elongation factor 1-alpha (TEF-1 alpha), confirmed the morphological identification. Furthermore, sequence data of actin genes from N. dimidiatwn was, for the first time, deposited to the GenBank. Koch's postulates were fulfilled by testing the susceptibility of different tomato tissues (leaves, stems, inner stems and roots of tomato seedlings, and detached tomato fruits and flowers) to N. dimidiatum inoculation. To our knowledge, this is the first report of N. dimidiatum on tomato.

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