Kızıltepe Meslek Yüksekokulu

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Citation - WoS: 10
Citation - Scopus: 18
Activity of Metalaxyl-M plus mancozeb, Fosetyl-Al, and Phosphorous Acid against Phytophthora Crown and Root Rot of Apricot and Cherry Caused by Phytophthora palmivora
(CZECH ACADEMY AGRICULTURAL SCIENCES, 2017) Derviş, Sibel; Derviş, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Crown and root rot, caused by the Oomycete pathogen Phytophthora palmivora, has become a destructive disease of apricot and cherry in eastern Turkey. There are no currently registered fungicides labeled for its control. In greenhouse experiments conducted in 2012 and 2013, 1-year-old potted apricot rootstock Zerdali and cherry rootstock Mahaleb plants were treated either with foliar spray of fosetyl-Al (140, 160, and 180 g a.i./100 l) or phosphorous acid (187.5, 200, and 215 g a.i./100 l) or soil drench of 100 ml of metalaxyl-M (= mefenoxam)+mancozeb (12+192, 16+256, and 20+320 g a.i./100 l) one day after wound inoculation of crowns and roots. In both years, phosphorous acid at 200 and 215 g a.i./100 l, fosetyl-Al at 160 and 180 g a.i./100 l, and metalaxyl-M+mancozeb at 20+320 g a.i./100 l significantly reduced the root rot severity on Zerdali by 70.68-80.00% and crown rot severity on both Zerdali and Mahaleb, by 68.32-91.96 and 74.21-82.60%, respectively, compared with phosphorous acid at 187.5 g a.i./100 l, fosetyl-Al at 140 g a.i./100 l, metalaxyl-M+mancozeb at 12+192 and 16+256 g a.i./100 l and control. Moreover, fosetyl-Al at 180 g a.i./100 l and metalaxyl-M+mancozeb at 20+320 g a.i./100 l significantly reduced the root rot severity on Mahaleb compared to fosetyl-Al at 140 and 160 g a.i./100 l, metalaxyl-M+mancozeb at 12+192 and 16+256 g a.i./100 l, phosphorous acid treatments and control in 2012, providing the best control of the disease by 88.00-90.68%. Two/three phosphorous acid foliar applications at 200 g a.i./100 l suppressed symptom development when field applications were made on a curative basis in 2014 and 2015.
Citation - Scopus: 1
Activity of nanosized copper-boron alloys against Phytophthora species
(Springer Science and Business Media Deutschland GmbH, 2023) Derviş, Sibel; Türkkan, Muharrem; İlhan, Hasan; Şimşek, Tuncay; Güler, Ömer; Derviş, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study aimed to evaluate the antifungal activity of copper-boron (Cu-B) nanoalloys against a range of Phytophthora species, including P. capsici, P. citrophthora, P. palmivora, P. cinnamomi, P. nicotianae, P. cactorum, P. plurivora, P. inundata, and P. megasperma. The nanoalloys were synthesized via mechanical alloying under an argon atmosphere, resulting in the formation of nanocrystalline Cu-B nanoalloys with irregular morphology and particle sizes ranging from 50 to 240 nm. At a concentration of 250 µg mL−1, the Cu-B nanoalloys demonstrated complete inhibition of mycelial growth, sporangium production, and zoospore germination in all tested Phytophthora species. The EC50 values for mycelial growth ranged from 28.02 to 120.17 µg mL−1, while for sporangium production and zoospore germination, they were below 10 µg mL−1. Furthermore, the nanoalloys exhibited fungicidal activity against specific Phytophthora species, such as P. capsici, P. citrophthora, P. inundata, and P. megasperma, at concentrations of 100, 250, 250, and 250 µg mL−1, respectively. Notably, the Cu-B nanoalloys displayed significant protective and curative effects on tuber rot severity in P. nicotianae-inoculated potatoes, resulting in reductions of 94.13% and 92.61% compared to the control, respectively, at a concentration of 10 µg mL−1 (P < 0.05). These findings highlight the potential of Cu-B nanoalloys as a promising fungicide for the management of plant diseases caused by Phytophthora spp.
Ağır Metal (Ni, Cd ve Cu) Uygulamalarının Andız Otu, Fener Otu ve Sığırkuyruğu Bitkilerinin Büyüme ve Gelişmesi Üzerine Etkisi
(2017) Eren, Abdullah; Mert, Mehmet; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Bu çalışmada; nikel (Ni), kadmiyum (Cd) ve bakır (Cu) ile kirlenmiş topraklarda andız otu (Inula helenium), fener otu (Physalis angulata) ve sığırkuyruğu (Verbascum thapsus) bitkileri 6 hafta boyunca kontrollü koşullarda yetiştirilerek, bu metallerin topraklardan temizlenebilme olanakları araştırılmıştır. Denemede bitkilerin klorofil düzeyleri, biyokütle üretimi, ağır metal alımı ve indirgenmiş glutatyon (GSH) konsantrasyonları belirlenmiştir. Elde edilen bulgulara göre, bitkilerin dokularında biriktirdiği Ni, Cd ve Cu konsantrasyonlarının toksisiteye neden olacak seviyelere ulaşmadığı tespit edilmiştir. En yüksek Ni içeriği (253 µg bitki-1) fener otu bitkisinin 400 mg Ni kg-1uygulamasından, en yüksek Cd içeriği ise fener otu (46.9 µg bitki-1) ve sığırkuyruğu (54.6 µg bitki-1) bitkilerinde 10 mg Cd kg-1uygulamasından ve en yüksek Cu konsantrasyonu ise fener otu (304 µg bitki-1) bitkisinin 200 mg Cu kg-1uygulamasından elde edilmiştir. Deneme sonucunda fener otu ve sığırkuyruğu bitkilerinin ağır metallerle kirlenmiş toprakların temizlenmesinde kullanma potansiyellerinin olduğu tespit edilmiştir.
Citation - WoS: 12
Citation - Scopus: 10
Alternaria alternata causing inner black rot of lemon (Citrus limon) fruits in Turkey: Genetic diversity and characterisation
(ScienceDirect, 2023) Derviş, Sibel; Derviş, Sibel; Fatih Tekin, Tacettin Utku Günen, Göksel Özer; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The market sales of symptomless lemons in 2020 revealed a persistent and severe internal black rot with browning of the membrane wall and juice sac. To investigate the causal agent, externally symptom-free lemons from Mediterranean locations were sampled from supermarkets, local markets, and bazaars in three southeastern Turkish provinces. Internal black rot symptoms were observed in 13.69% of the 1300 externally symptomless fruits, and Alternaria was isolated from 96.63% of those exhibiting these symptoms. Morphological analysis confirmed the identity of Alternaria alternata in 26 representative isolates collected from various markets and bazaars. To examine genetic diversity or polymorphisms, start codon targeted (SCoT) markers were used. Species identification was based on the sequences of translation elongation factor 1-alpha (TEF 1-α), RNA polymerase second largest subunit (RPB2), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). SCoT 29 exhibited the highest amount of polymorphic amplicons, with 18 polymorphic bands scored when compared to the other six scoreable markers. The 26 representative isolates from various markets and bazaars were divided into four clades by SCoT 29. However, multilocus sequence analyses confirmed the identification of a single species, A. alternata. In terms of virulence and the impact of temperature on growth, representative isolates from four SCoT clusters were evaluated. Data suggests that SCoT markers can be used to evaluate virulence-based polymorphism in A. alternata; nonetheless, all clades showed comparable temperature responses, with 25 ◦C being the optimum. There was also no association between individual morphotypes and SCoT clade membership. Due to the lack of obvious signs on lemon fruits, producers, merchants, and consumers are unable to identify black rot, and the widespread presence of A. alternata in lemons may pose a significant risk that must be avoided at all costs.
Alternaria spp. causing postharvest decay on apple fruit in Nigde province, Turkey
(2022) Derviş, Sibel; Derviş, Sibel; Özden, Mustafa; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Postharvest rots caused by Alternaria spp. result from infections occurring in the apple orchard at or before harvest and these may remain quiescent during the growing phase and only result in decay during postharvest cold storage. Therefore, the objective of this study was to identify and characterize Alternaria spp. associated with apple black spot and/ or decay during cold storage in Nigde, Turkey. Isolates of Alternaria spp. were obtained from apple fruit (cvs Fuji, Golden Delicious, Granny Smith, Red Delicious, and Starking Delicious) with dark, dry, spongy lesions. The symptomatic fruits were sampled from 18 commercial cold storages in the Nigde Province, Turkey, during the 2020/21 storage period. Decayed fruits were transferred to the laboratory for isolation of the fungi from the lesions. In total, 75 Alternaria isolates were characterized morphologically and were differentiated into 5 phenotypic groups based on their colony morphology, and characteristics of their conidiophores and conidia. At least 5 representative isolates were randomly chosen from each group for the pathogenicity tests. All Alternaria spp. isolates caused round, dry, dark, spongy lesions on/in the inoculated fruits and re-isolates from the inoculated apples of each phenotypic groups were similar to the initial isolates of each group in morphological characteristics. Molecular characterization of isolates is in progress. Since mycotoxin produced by Alternaria spp. in fruits is of unavoidable risk to human health, special care should be given in regards to the need to reduce widespread postharvest losses caused by Alternaria spp. in the cold storages of Niğde, which is the most important apple producer province of the country.
Antifungal Activity of Some Lactic Acid Bacteria Against Several Soilborne Fungal Pathogens Isolated from Strawberry Plants
(Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 2018) Derviş, Sibel; Doğaner, Müzeyyen Müge; Derviş, Sibel; Ulubaş Serçe, Çiğdem; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Developing as an alternative plant disease control method by using beneficial microorganisms and their metabolites has gained so much importance in recent years. In this study, the possibilities of using microorganisms which have potential antimicrobial effects on controlling soil-borne fungi at strawberry production were investigated. Effects of different lactic acid bacteria (LAB) strains on the development of several soil-borne fungi were studied in vitro and in vivo. LAB were screened for antifungal activity by using cell free supernatant against Fusarium sp., Rhizoctonia sp., Macrophomina sp., Botrytis sp., Phtopythium sp., Cylindrocarpon sp. and Pestalotiopsis sp. Cell free supernatant of LAB isolates showed promising antifungal activity. In vitro effective strains of LAB were tested in pot experiments to search their effects on disease development and plant growth. While the antifungal effects of all LAB strains tested in vitro experiments exhibited promising results, in vivo experiments revealed similar effects on different fungi genera.
Antifungal effect of boron compounds against Neoscytalidium dimiatum
(2023) Derviş, Sibel; Türkkan, Muharrem; Yiğit, Abdurrahman; Derviş, Sibel; Özer, Göksel; Erper, İsmail; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Neoscytalidium dimidiatum has been recently identified as the agent responsible for canker, dieback, shoot blight, and root rot on different hosts in Türkiye. In this study, we evaluated the inhibitory effect of boric acid (H3BO3), three borates [disodium octaborate tetrahydrate (Na2B8O13.4H2O), disodium tetraborate decahydrate (Na2B4O7.10H2O) and disodium tetraborate (Na2B4O7)] on the mycelial growth, germ tube elongation and conidial germination of N. dimidiatum. The antifungal effects of the boron compounds were investigated at 0.125, 0.25, 0.5, 1.0 and 2.0% (w/v) concentrations. The differences observed between the inhibitory effects of boron compounds on the parameters were found to be significantly important at P < 0.05. The 0.5% concentration of disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited the fungus, whereas other salts did not. Disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited three parameters at 0.5% and higher concentrations. However, boric acid and disodium tetraborate were able to completely inhibit investigated factors of N. dimidiatum at 2.0% and 1.0% concentration, respectively. Disodium tetraborate and boric acid at 0.5% concentration decreased germ tube elongation and conidia germination of the fungus by 94.97%-63.57%, 59.33%-51.26%, respectively. The effectiveness of the 0.5% concentration on conidial germination was also similar in both salts at P < 0.05. However, disodium tetraborate inhibited germ tube elongation more effectively than boric acid. The minimum inhibition concentration (MIC) values of the four boron compounds also varied between 0.5% and 2.0% concentrations. The boron compounds in this study could be potential agents to manage N. dimidiatum.
Bakırla Kirlenmiş Toprakların Xanthium strumarium L. Bitkisi Kullanılarak Fitoremediasyonu
(Türkiye Tarımsal Araştırmalar Dergisi, 2018) Eren, Abdullah; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Antropojenik aktivite sonucu çevreye dâhil olan ağır metaller, toprak kirliliğine ve toprak kalitesinin düşmesine neden olmaktadırlar. Ağır metallerin topraktan temizlenmesi için en uygun yöntemlerden biri olan fitoremediasyon yöntemi çevre dostu ve ekonomik bir yöntemdir. Bu çalışma, yapay olarak bakır (Cu) ile kirlenmiş topraktan, Xanthium strumarium L. (pıtrak) bitkisinin, fitoekstraksiyon etkinliğini belirlemek amacıyla yapılmıştır. Farklı dozlarda (0, 100, 200, 400 ve 800 mg Cu kg-1) Cu uygulanan toprakta 45 gün boyunca sera koşullarında pıtrak (Xanthium strumarium L.) bitkisi yetiştirilmiştir. Bitkilerin başta Cu konsantrasyonu olmak üzere klorofil içeriği, biyokütle üretimi, indirgenmiş glutatyon (GSH), bazı makro [azot (N), fosfor (P), potasyum (K), kalsiyum (Ca) ve magnezyum (Mg)] ve mikro [demir (Fe), çinko (Zn) ve manganez (Mn)] besin elementi konsantrasyonları ölçülmüştür. Bakır uygulanmış bitkiler kontrol (0 mg Cu kg-1) bitkisi ile karşılaştırılmıştır. Artan dozda Cu uygulamaları, Cu ve GSH konsantrasyonları hariç diğer ölçülen parametrelerde bir azalmaya neden olmuştur. Sonuçlar, pıtrak bitkisinin, Cu ile kirlenmiş toprakların temizlenmesi için kullanılabileceğini ve fitoremediasyon yöntemine uygun olduğunu göstermiştir.
Biber (Capsicum annum L.) Bitkisinden İzole Edilen Endofitik Bakteri ve Topraktan İzole Edilen Bakterilerin Toprak Kökenli Fusarium solani, F. oxysporum, Macrophomina phaseolina ve Rhizoctonia solani’xxye Karşı Antagonistik Aktivitelerinin Belirlenmesi
(2017) Güler Güney, İnci; Güldür, Mehmet Ertuğrul; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Forty-nine endophytic bacteria were isolated from pepper stem and crown and forty-nine bacteria from soil. Isolates were obtained from pepper grown fields of Bozova and Hilvan Districts of Şanlıurfa. Antagonistic effects of endophytic bacteria and soil-borne bacteria were in-vitro tested against Fusarium solani, F. oxysporum, Macrophomina phaseolina and Rhizoctonia solani in petri dishes. Bacterial isolates from soil and endophytic bacteria revealed antagonistic activity of 79.37-20.22% and 81.69-33.47%, respectively against four pathogens.Statistically significant differences were found among the efficiencies against four different pathogens. Additionally, bacteria isolated from Bozova province were found to have higher antagonistic activity than those of Hilvan province. The antagonistic activity of endophytic bacteria was found to be higher than that of bacterial isolates from soil. In later studies, effective endophytic bacteria isolated from pepper can be used against soilborne pathogens
Bipolaris sorokiniana associated with common root rot in Azerbaijan and Kazakhstan
(2022) Derviş, Sibel; Bozoğlu, Tuğba; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, Göksel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Wheat (Triticum spp.) is the third most important crop in terms of global production, with an average annual production of almost 219 million ha and yielding 760.9 million tons, after maize and rice. However, A complex of fungi attacking the crown and root tissues of wheat causes a serious problem and significant yield reductions in wheat. Although most prevailing species in this complex can change yearly and regionally in dryland winter wheat production areas, Bipolaris sorokiniana Shoemaker (teleomorph: Cochliobolus sativus) is one of the dominant species and causes spot blotch and common root rot (CRR) on wheat plants, especially in winter wheat varieties. Comprehensive surveys were conducted to identify B. sorokiniana associated with CRR of wheat throughout the main wheat-growing areas of Azerbaijan and Kazakhstan in 2017 and 2019, respectively. As a result of the classification of species based on morphological and molecular tools, 96 and 547 isolates were identified as B. sorokiniana for Azerbaijan and Kazakhstan, respectively. All surveyed regions for these countries were contaminated with this pathogen. Some representative isolates of B. sorokiniana were tested for their ability to cause disease and produced moderate disease severities on cultivar Seri 82 (Triticum aestivum, bread wheat). The percent incidence of B. sorokiniana isolates in all isolates was 15.2% for Azerbaijan, whereas, for Kazakhstan, B. sorokiniana was the most frequently recovered species in the three regions surveyed, with an isolated frequency of 44.80%. The results of the current study provide crucial and helpful information to improve disease management strategies against CRR of wheat in these countries.
Bitki Gelişimini Teşvik Eden Bazı Mikroorganizmalar
(2009) Güler Güney, İnci; Güler Güney, İnci; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Bitki gelişimini arttıran Rhizobium spp. , Azospirillum spp. ve Glomus spp. gibi mikroorganizmalar hastalık kontrolünde de etkili olmaktadır. Pseudomonas spp. ve Trichoderma spp. gibi biyolojik mücadele etmenlerinin ise bitki gelişimini teşvik ettikleri son yıllarda yapılan çalışmalarda ortaya konmuştur. Tarımsal ürünleri hastalıklara karşı korumak ve gelişimlerini arttırmak için, bu mikroorganizmaların uygun zamanda ve miktarlarda kullanımları sağlanmalıdır. Böylece kimyasal gübrelerin kullanımları sonucu oluşan problemler azaltılabilir. Bu derlemede, biyolojik gübre etmeni olarak kullanılan mikroorganizmaların hem bitki gelişimini teşvik etmeleri hem de biyolojik mücadelede kullanılmaları ile ilgili yapılan çalışmalar özetlenmiştir.
THE CADMIUM PHYTOEXTRACTION EFFICIENCY OF SCMTII GENE BEARING TRANSGENIC TOBACCO PLANT
(Biotechnology & Biotechnological Equipment, 2010) Eren, Abdullah; Arslan, Mehmet; Uygur, Veli; Köleli, Nurcan; Eren, abdullah; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Cadmium pollution is a serious world-wide problem affecting the human health and the environmental quality. Phytoremediation, the use of green plants to remove, sequester or detoxify pollutants offers an environmentally-friendly alternative to engineeringbased methods for remediation. The T2 generations of the ScMTII gene bearing transgenic and non-transgenic tobacco plants were grown hydroponically in Hoagland nutrition solution containing 0, 5 and 10 mg/L Cd in controlled growth room to determine their ability to uptake and accumulate Cd within the shoots and roots. There were no significant shoot and root dry weight differences between transgenic and non-transgenic tobacco plants. The ScMTII gene bearing transgenic tobacco plant accumulated 19.8% higher Cd than the non-transgenic tobacco plant in the above ground parts of the plant during the two weeks exposure period in hydroponic culture. In non-transgenic plant, however, Cd is accumulated mainly in the roots. The results of current study indicate that the use of the ScMTII gene bearing transgenic tobacco plant for Cd phytoremediation is limited. Further studies are needed to test the effectiveness of the ScMTII gene for phytoextraction of other heavy metal ions.
Citation - WoS: 18
Citation - Scopus: 20
Canker and leaf scorch on olive (Olea europaea L.) caused by Neoscytalidium dimidiatum in Turkey
(ScienceDirect, 2022) Derviş, Sibel; Özer, Göksel; Türkölmez, Şahimerdan; Derviş, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In a recent survey of olive groves in the Southeast Anatolia Region of Turkey, a new and serious decline of olive trees, beginning with foliar scorching and then dieback of twigs, branches, and even whole trees, was observed for the first time. In more advanced stages of the disease, necrosis and cankers were observed on the bark of the trunk, branches, and twigs. Isolations from symptomatic tissues from multiple cultivars in diverse locations yielded Neoscytalidium dimidiatum, which were identified using ITS, tef1, and tub2 loci of genomic DNA, in combination with morphological data. In vitro studies showed that conidial germination, hyphal growth, and pycnidia formation of the pathogen were positively correlated with elevated temperatures. Wild type olive “Delice”, “Edincik Su”, and “Memecik” seemed like the most resistant cultivars on which disease severity values were the lowest among 14 screened olive cultivars in two experimental orchards under natural conditions. Pathogenicity tests showed that “Gemlik” was the most susceptible cultivar showing the largest cankers and extensive scorch lesions. Isolates caused canker but not leaf scorch on O. europaea cultivars “Arbequina” “Halhalı”, “Manzanilla”, “Nizip Yağlık”, and “Saurani”. Neoscytalidium isolates are likely to have a negative impact on the health of diverse olive groves, which are primarily confined to Mediterranean-type climatic regions. These findings suggest an increased risk of infection in environments with increasing temperatures, as is common in the Southeast Anatolia Region of Turkey. The information gathered in this study will be used to examine the disease's epidemiology and establish disease control initiatives. This is also the first report of N. dimidiatum infecting O. europaea in the world.
Citation - WoS: 16
Citation - Scopus: 16
Characterization and pathogenicity of Pythium-like species associated with root and collar rot of kiwifruit in Turkey
(In Plant Disease, 2021) Derviş, Sibel; Özer, Göksel; Karaca, Gürsel; Erper, İsmail; Derviş, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
During the period of June to October in 2018, a widespread decline was observed on kiwifruit vines in the vineyards located in Altınordu, Fatsa, and Perşembe districts of Ordu province. The symptoms were associated with reddish-brown rots expanding from the root to the collar with sparse off-color foliage. Based on the percentage of the total infected samples across 18 vineyards, the most common oomycete species were Globisporangium intermedium (37.1%), Phytopythium vexans (34.3%), G. sylvaticum (14.3%), G. heterothallicum (11.4%), and Pythium dissotocum (2.9%). The morphological identification of isolates was confirmed based on partial DNA sequences containing the nuclear rDNA internal transcribed spacer region (rDNA ITS) and the mitochondrial cytochrome c oxidase subunit II (coxII) gene. The optimum growth temperature and the optimum pH of 5 species ranged from 22.98 to 28.25°C and 5.67 to 8.51, respectively. Pathogenicity tests on the seedlings of kiwifruit cv. Hayward revealed significant differences in virulence among isolates. Phytopythium vexans and G. sylvaticum isolates caused severe root and collar rot resulting in seedling death, while G. heterothallicum and G. intermedium isolates had relatively lower virulence. All Globisporangium spp. and P. vexans isolates significantly decreased plant growth parameters (plant height, shoot and root dry weights and root length); however, P. dissotocum caused very mild symptoms and did not affect these parameters of growth. To our knowledge, this is the first study reporting G. sylvaticum, G. heterothallicum, and G. intermedium causing root and collar rot on kiwifruit not only in Turkey but also in the world.
Citation - WoS: 5
Citation - Scopus: 8
Clonostachys rosea Strain ST1140: An Endophytic Plant-Growth-Promoting Fungus, and Its Potential Use in Seedbeds with Wheat-Grain Substrate
(SpringerLink, 2023) Derviş, Sibel; Özer, Göksel; Derviş, Sibel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In this study, Clonostachys rosea strain ST1140, a naturally occurring endophyte in healthy roots of a pistachio tree, was identifed morphologically and molecularly through DNA sequencing, and its endophytic ability and growth efect in/on three solanaceous plant species were tested. Three diferent organic substrates (bread wheat-grain, sawdust, and leonardite) were also investigated for their utility in cultivating and multiplying the strain. In-tray and pot experiments, the rates of seed germination and vegetative development of pepper, tomato, and eggplant inoculated with C. rosea at planting were compared to those of non-inoculated controls. In pot experiments, inoculating seedbeds with increasing doses of C. rosea strain ST1140 with wheat-grain substrate resulted in higher plant height values for all plant species, and the strain endophytically colonized the roots of all plant species. In-tray experiments revealed that ST1140 inoculation resulted in 2–4 days earlier and 10% higher germination rates for all plant species, as well as more vigorous and accelerated seedling growth (10–13 days earlier for seedlings at the 4–5 true leaf stage) on all inoculated plant species. Among three diferent organic substrates, wheat-grain was found to be the most efective for long-term cultivation and multiplication of the fungus, which could be of interest for its development as a commercial product. These results promised the strain ST1140’s use as a biofertilizer in seedbeds with a wheat-grain substrate.
Correction to: Leaf spot caused by Alternaria crassa on Datura stramonium in Turkey
(Australasian Plant Disease Notes, 2022) Derviş, Sibel; Alkan, Mehtap; Derviş, Sibel; Özer, Göksel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In August 2021, jimson weed (Datura stramonium) plants growing as weeds in potato fields in Bolu province, Turkey, exhibited leaf spots with dark concentric rings. Sunken and lens-shaped lesions with a light center were also frequently observed on petioles, branches, and stems. Based on morphological characteristics and phylogenetic analysis of the transcription elongation factor 1-α, RNA polymerase second largest subunit, and glyceraldehyde-3- phosphate dehydrogenase loci, the causal agent was identified as Alternaria crassa. The pathogen was successfully re-isolated from inoculated jimson weed plants in the pathogenicity assay, proving Koch’s postulates. Alternaria crassa caused necrotic lesions on potato plants, similar to those of early blight, confirming them as an alternative host of the pathogen. This is apparently the first report of leaf spot caused by A. crassa on jimson weed in Turkey.
Diyarbakır Yöresindeki Bazı Tarım Topraklarının Hümik Asit İçerikleri ile Eser Elementler Arasındaki İlişkinin Çok Değişkenli İstatistiksel Yöntemlerle Değerlendirilmesi
(Kahramanmaraş Sütçü İmam Üniversitesi, 2024) Eren, Abdullah; Eren, Abdullah; Bilge, Uğur; Ceylan, Ramazan; Selçuk, Ramazan; Düz, M. Zahir; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Hümik asit (HA) ile topraktaki iz elementlerin biyoyarayışlılığı, reaksiyonları ve hareketliliği arasında önemli ilişkiler bulunmaktadır. Bu nedenle topraklar, HA uygulamalarıyla kimyasal, biyolojik ve fiziksel yönden iyileştirilmeye çalışılmaktadır. Bu çalışmada, Diyarbakır yöresinden 118 adet tarımsal toprak örneklerinin hümik asit içeriklerinin bazı iz elementlerle (Al, As, Ba, Be, Cd, Fe, Mn, Pb, Sb, Sn, Se, V ve P) olan ilişkisi çok değişkenli istatistiksel analizlerle değerlendirilmiştir. Toprak örnekleri mikrodalga yaş yakma yöntemiyle çözünürleştirildikten sonra element içerikleri ICP OES (Inductively Coupled Plasma Optical Emission Spectrometer) cihazı ile belirlenmiştir. Yöntemin doğruluğu için SRM (Standard Reference Material) olarak SRM NIST 2586 kullanılmıştır. Yapılan analiz sonucunda geri kazanım değerleri %91.6 ile %105.9 arasında bulunmuştur. Hümik asit topraklardan International Society for Humic Substances (IHSS) yöntemiyle ekstrakte edilmiş, çalkalayıcı ve santifrüj cihazı kullanılarak belirlenmiştir. Yöntemin doğruluğu için Hümik Asit Sodyum Tuzu (HA-Na) ile test edilmiştir. Elde edilen veri setine Pearson korelasyonu ve kısmi korelasyon analizi uygulanmıştır. Ayrıca çoklu regresyon, HCA (Hiyerarşik Küme Analizi) ve PCA (Principal Component Analysis) gibi çok değişkenli istatistiksel analizler uygulanmıştır. Çoklu regresyon analizi Step-wise yöntemine göre yapılmıştır. Manganese ve P (p< 0.01), HA bağımlı değişken olarak alındığında önemli bulunmuştur. Pearson korelasyon katsayısına göre toprakta HA ile As (r = -0.282**) arasındaki ilişki negatif ve anlamlı iken, Fe (r = 0.185*), Mn (r =0.273**)), Sn (r = 0.242*), Se (r = 0.325**) ve P ( r = 0.315**) gibi diğer elementlerle pozitif ve anlamlı olarak belirlenmiştir. Kümeleme ve PCA analizinde HA, P Mn ve Fe’nin aynı grupta olduğu saptanmıştır. Yapılan analizler HA’in toprağın bitki besin elementleri lehinde pozitif etki yaptığını göstermiştir.
Dünya’da ve Türkiye’de Tıbbi ve Aromatik Bitkilerin Yeri ve Önemi
(Çukurova Tarım Gıda Bil. Der., 2018) Acıbuca, Veysi; Bostan Budak, Dilek; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Tarımsal faaliyetlerde, insanoğlu için hayati önem taşıyan beslenme, giyinme, barınma gibi ihtiyaçların karşılanması için gerekli ürünlerin üretimi yapıldığı gibi, insan sağlığı için faydalı olan ürünlerin de üretimi yapılmaktadır. İnsanlık tarihinin başlangıcından beri tedavi edici özelliği bulunan bitkilerin, hastalıklarda kullanıldığı bilinmektedir. Dünya Sağlık Örgütü verilerine göre gelişmemiş olan ülkelerde nüfusun % 80’i tedavi amaçlı geleneksel ilaçlar kullanırken, bu oran gelişmiş ülkelerde % 40 civarında olup gelecekte tıbbi bitkilerden yararlanma oranının tüm dünyada artış göstermesi beklenmektedir. Bu çalışmada ulusal ve uluslararası kurumlar ile konu ile ilgili daha önceden yapılmış çalışmalardan elde edilen veriler doğrultusunda tıbbi ve aromatik bitkilerin Dünya ve Türkiye’deki üretim durumu ve ticareti incelenmiştir.
The Effect of Talaromyces funiculosus ST976 Isolated from Pistacia vera Rhizosphere on Phosphorus Solubility in Soil Samples with Different Physicochemical Properties
(Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 2022) Derviş, Sibel; Eren, Abdullah; Eren, Abdullah; Özer, Göksel; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In this study, a total of 78 Talaromyces isolates were isolated from the pistachio (Pistacia vera L.) rhizosphere heavily infested with Neoscytalidium spp. The identification studies of the four representative isolates based on morphological and molecular methods showed that all isolates were T. funiculosus. The 575 bp long sequence of the internal transcribed spacer region of T. funiculosus isolate ST976, selected as a representative of the isolates, was deposited in GenBank under accession no. MW130842. The Maximum Likelihood tree clustered the ST976 isolate with reference T. funiculosus isolates derived from the GenBank nucleotide database. The phosphorus dissolution ability of ST976 isolate was determined by an experiment using six soil samples collected from agricultural lands in various locations of Şanlıurfa province. The pH of the soil samples taken varied between 7.21 and 7.88. As a result of the analysis performed with the addition of the isolate ST976 applied to soil samples with different soil structures (Clay and Clay-Loam), it was determined that the isolate ST976 dissolved 109–311% more phosphorus than the control sample. The study is one of the first studies proving the ability of T. funiculosus isolate ST976 to dissolve phosphorus without any additives to soil solution was determined.
THE EFFECTS OF NICKE L APPLICATIONS ON TH E GROWTH OF COCKLEBUR ( XANTHIUM STRUMARIUM L.) PLANT
(APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, 2019) Eren, Abdullah; 16.01. Department of Organic Agriculture / Organik Tarım Bölümü; 16. School of Vocational Higher School of Kızıltepe/ Kızıltepe Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Nowadays the environmental pollution caused by heavy metals is spreading all over the world, especially where the industry is developing rapidly, and soils are polluted very dramatically and hazardously at a high level. In this study, cocklebur-(Xanthium strumarium L.) plants were grown in nickel (Ni) contaminated soil (0, 50, 100, 200 and 400 mg Ni kg-1) under greenhouse conditions for 6 weeks to study the ability of Ni uptake and accumulation of the plants. The Ni treated plants were compared with the control (0 mg Ni kg-1) plant. As a result of that comparison, chlorophyll levels of old and young leaves, dry weights, reduced glutathione (GSH), macronutrient concentrations, such as nitrogen (N), phosphor (P), potassium (K), calcium (Ca) and magnesium (Mg)) and micronutrient concentrations, such as iron (Fe), zinc (Zn), and manganese (Mn) nutrient concentrations of the plants were decreased, whilst the concentrations of Ni and of copper (Cu) increased with increasing dose of Ni application. The results show that the studied plant (Xanthium strumarium L.) can be used for the cleaning up of Ni-contaminated soils and is suitable for phytoremediation.

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