Browsing by Author "Özer, Göksel"

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Citation - WoS: 5
Citation - Scopus: 3
Leaf spot caused by Alternaria crassa on Datura stramonium in Turkey
(SpringerLink, 2022) Bozoğlu, Tuba; Alkan, Mehtap; Derviş, Sibel; Özer, Göksel
In August 2021, jimson weed (Datura stramonium) plants growing as weeds in potato felds in Bolu province, Turkey, exhibited leaf spots with dark concentric rings. Sunken and lens-shaped lesions with a light center were also frequently observed on petioles, branches, and stems. Based on morphological characteristics and phylogenetic analysis of the transcription elongation factor 1-α, RNA polymerase second largest subunit, and glyceraldehyde-3- phosphate dehydrogenase loci, the causal agent was identifed as Alternaria crassa. The pathogen was successfully re-isolated from inoculated jimson weed plants in the pathogenicity assay, proving Koch’s postulates. Alternaria crassa caused necrotic lesions on potato plants, similar to those of early blight, confrming them as an alternative host of the pathogen. This is apparently the frst report of leaf spot caused by A. crassa on jimson weed in Turkey
Citation - Scopus: 5
First report of Globisporangium heterothallicum causing root and crown rot of pepper in Turkey
(New Disease Reports, 2020) Derviş, Sibel; Özer, Göksel; Türkölmez, Şahimerdan; Çiftçi, Osman
Turkey is the world's third-largest producer of pepper (Capsicum annuum), annually cultivating over 90,000 ha and producing over half a million tonnes of fruit. In 2019, wilting and death of c. 20% of plants were observed in pepper fields in Şanlıurfa province, Turkey (Fig. 1). Severe root and crown rot symptoms with discoloration were observed on affected plants, and necrotic lesions expanded rapidly into the stems which killed the plant (Fig. 2).
Citation - WoS: 4
Citation - Scopus: 4
First report of Macrophomina phaseolina causing charcoal rot on common sage (Salvia officinalis) in Turkey
(SpringerLink, 2021) İslim, Koşar; Güney, İnci Güler; Derviş, Sibel; Kırlı, Onur; Özer, Göksel
Common sage (Salvia officinalis L.) is a perennial herb or sub-shrub native to the eastern Mediterranean. In July 2020, symptoms of chlorosis, wilting, and root rot appeared in 25% of two-year-old S. officinalis cv. Elif plants in two fields (N 36°53'42.457'', E 38°55'34.777''; N 36°53'27.236'', E 38°55'38.618'') in Şanlıurfa, Turkey.
Citation - WoS: 24
Citation - Scopus: 29
Fungal Pathogens Associated with Crown and Root Rot of Wheat in Central, Eastern, and Southeastern Kazakhstan
(Journal of Fungi, 2022) Bozoğlu, Tuğba; Derviş, Sibel; Imren, Mustafa; Amer, Mohammed; Özdemir, Fatih; Paulitz, Timoth; Morgounov, Alexey; Dababat, Abdelfattah A.; Özer, Göksel
Kazakhstan is the fourteenth largest wheat producer in the world. Despite this fact, there has not been a comprehensive survey of wheat root and crown rot. A quantitative survey was conducted for the purpose of establishing the distribution of fungi associated with root and crown rot on wheat (Triticum spp.). During the 2019 growing season, samples were taken from the affected plants’ roots and stem bases. A total of 1221 fungal isolates were acquired from 65 sites across the central (Karagandy region), eastern (East Kazakhstan region), and southeastern (Almaty region) parts of the country and identified using morphological and molecular tools. The internal transcribed spacer (ITS), translation elongation factor 1-alpha (EF1-α), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequences were successfully used to identify the species of fungal isolates. It was found that Bipolaris sorokiniana (44.80%) and Fusarium acuminatum (20.39%) were the most predominant fungal species isolated, which were present in 86.15 and 66.15% of the fields surveyed, respectively, followed by F. equiseti (10.16%), Curvularia spicifera (7.62%), F. culmorum (4.75%), F. oxysporum (4.10%), F. redolens (2.38%), Rhizoctonia solani AG2-1 (1.06%), Nigrospora oryzae (0.98%), C. inaequalis (0.90%), F. pseudograminearum (0.74%), F. flocciferum (0.74%), Macrophomina phaseolina (0.66%), F. cf. incarnatum (0.33%), Fusarium sp. (0.25%), and F. torulosum (0.16%). A total of 74 isolates representing 16 species were tested via inoculation tests on the susceptible Triticum aestivum cv. Seri 82 and the results revealed that F. culmorum and F. pseudograminearum, B. sorokiniana, Fusarium sp., R. solani, F. redolens, C. spicifera, C. inaequalis, and N. oryzae were virulent, whereas others were non-pathogenic. The findings of this investigation demonstrate the presence of a diverse spectrum of pathogenic fungal species relevant to wheat crown and root rot in Kazakhstan. To the best of our knowledge, this is the first report of F. pseudograminearum, Fusarium sp., C. spicifera, and C. inaequalis as pathogens on wheat in Kazakhstan.
Citation - WoS: 2
Citation - Scopus: 2
First report of needle blight of blue spruce (Picea pungens) caused by Neoscytalidium dimidiatum in Turkey
(SpringerLink, 2023) Derviş, Sibel; Türkölmez, Şahimerdan; Güney, İnci Güler; Alkan, Mehtap; Özer, Göksel
The blue spruce (Picea pungens Engelm.) is an exotic conifer species widely used for decorative purposes in landscaping and private gardens. In 2020, 60% of forty blue spruce trees between 20 and 25 years old planted in front of buildings in Kavaklıdere, Ankara, displayed needle blight symptoms. Needles on the lower and interior crown closest to the trunk were infected from the bottoms of branches. They showed a brown-colored or burned appearance before dropping off, starting from their tips, resulting in up to a 65% reduction of the canopy. On the surface of infected tissues, a black-colored fungal mass was observed. Fungal colonies isolated from twenty symptomatic branches and needles were initially dark gray and became black within 4 to 7 days. Zero- to one-septate dark brown conidia (4.6 to 9.8 × 3.6 to 7.5 μm) formed in arthric chains were disarticulating and cylindrical-truncate to rod-shaped, oblong, ellipsoidal, doliiform, or globose, consistent with the description of Neoscytalidium spp. (Crous et al. 2006). The internal transcribed spacer, translation elongation factor 1-α, and β-tubulin loci of a randomly selected isolate (Nd_Pp01) were sequenced using the primers ITS1/4, EF1-728 F/986R, and Bt2a/Bt2b and deposited in GenBank under the accession numbers OK643641, OK666381, and OK666382, respectively. The sequences had 99.77–100% nucleotide identity with those of the type specimen of N. dimidiatum, CBS 145.78. Phylogenetic analysis with concatenated sequences further confirmed the identification. The isolate was deposited in the Bolu Abant Izzet Baysal University Culture Collection with accession number BAIBU-179. A pathogenicity test was conducted with ten 1-year-old P. pungens plants by spraying a conidial suspension (105 conidia/ml) of the isolate Nd_Pp01 onto the needles (Türkölmez et al. 2019). Five seedlings sprayed with sterile water were used as controls. All plants were placed in a growth chamber at 28°C with 70% RH. Spray-inoculated needles initially showed yellow spots within two weeks and turned brown six weeks after inoculation, whereas the controls remained symptom-free. The pathogen was re-isolated from the inoculated needles but not from controls. To our knowledge, this is the first report of N. dimidiatum causing needle blight on P. pungens in Turkey and worldwide (Farr and Rossman 2023).
Antifungal effect of boron compounds against Neoscytalidium dimiatum
(2023) Yildirim, Elif; Türkkan, Muharrem; Yiğit, Abdurrahman; Derviş, Sibel; Özer, Göksel; Erper, İsmail
Neoscytalidium dimidiatum has been recently identified as the agent responsible for canker, dieback, shoot blight, and root rot on different hosts in Türkiye. In this study, we evaluated the inhibitory effect of boric acid (H3BO3), three borates [disodium octaborate tetrahydrate (Na2B8O13.4H2O), disodium tetraborate decahydrate (Na2B4O7.10H2O) and disodium tetraborate (Na2B4O7)] on the mycelial growth, germ tube elongation and conidial germination of N. dimidiatum. The antifungal effects of the boron compounds were investigated at 0.125, 0.25, 0.5, 1.0 and 2.0% (w/v) concentrations. The differences observed between the inhibitory effects of boron compounds on the parameters were found to be significantly important at P < 0.05. The 0.5% concentration of disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited the fungus, whereas other salts did not. Disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited three parameters at 0.5% and higher concentrations. However, boric acid and disodium tetraborate were able to completely inhibit investigated factors of N. dimidiatum at 2.0% and 1.0% concentration, respectively. Disodium tetraborate and boric acid at 0.5% concentration decreased germ tube elongation and conidia germination of the fungus by 94.97%-63.57%, 59.33%-51.26%, respectively. The effectiveness of the 0.5% concentration on conidial germination was also similar in both salts at P < 0.05. However, disodium tetraborate inhibited germ tube elongation more effectively than boric acid. The minimum inhibition concentration (MIC) values of the four boron compounds also varied between 0.5% and 2.0% concentrations. The boron compounds in this study could be potential agents to manage N. dimidiatum.
Citation - WoS: 17
Citation - Scopus: 18
Characterization and pathogenicity of Pythium-like species associated with root and collar rot of kiwifruit in Turkey
(In Plant Disease, 2021) Türkkan, Muharrem; Özer, Göksel; Karaca, Gürsel; Erper, İsmail; Derviş, Sibel
During the period of June to October in 2018, a widespread decline was observed on kiwifruit vines in the vineyards located in Altınordu, Fatsa, and Perşembe districts of Ordu province. The symptoms were associated with reddish-brown rots expanding from the root to the collar with sparse off-color foliage. Based on the percentage of the total infected samples across 18 vineyards, the most common oomycete species were Globisporangium intermedium (37.1%), Phytopythium vexans (34.3%), G. sylvaticum (14.3%), G. heterothallicum (11.4%), and Pythium dissotocum (2.9%). The morphological identification of isolates was confirmed based on partial DNA sequences containing the nuclear rDNA internal transcribed spacer region (rDNA ITS) and the mitochondrial cytochrome c oxidase subunit II (coxII) gene. The optimum growth temperature and the optimum pH of 5 species ranged from 22.98 to 28.25°C and 5.67 to 8.51, respectively. Pathogenicity tests on the seedlings of kiwifruit cv. Hayward revealed significant differences in virulence among isolates. Phytopythium vexans and G. sylvaticum isolates caused severe root and collar rot resulting in seedling death, while G. heterothallicum and G. intermedium isolates had relatively lower virulence. All Globisporangium spp. and P. vexans isolates significantly decreased plant growth parameters (plant height, shoot and root dry weights and root length); however, P. dissotocum caused very mild symptoms and did not affect these parameters of growth. To our knowledge, this is the first study reporting G. sylvaticum, G. heterothallicum, and G. intermedium causing root and collar rot on kiwifruit not only in Turkey but also in the world.
Citation - WoS: 13
Citation - Scopus: 13
Molecular phylogeny of plant pathogenic fungi based on start codon targeted (SCoT) polymorphism
(Springer, 2023) Palacıoğlu, Gülsüm; Alkan, Mehtap; Derviş, Sibel; Bayraktar, Harun; Özer, Göksel
Background: A number of molecular marker systems have been developed to assess genetic diversity, carry out phylogenetic analysis, and diagnose and discriminate plant pathogenic fungi. The start codon targeted (SCoT) markers system is a novel approach used here to investigate intra and interspecific polymorphisms of phytopathogenic fungi. Materials and methods: This study assessed genetic variability between and within 96 isolates of ten fungal species associated with a variety of plant species using 36 SCoT primers. Results: The six primers generated 331 distinct and reproducible banding patterns, of which 322 were polymorphic (97.28%), resulting in 53.67 polymorphic bands per primer. All primers produced informative amplification profiles that distinguished all fungal species. With a resolving power of 10.65, SCoT primer 12 showed the highest polymorphism among species, followed by primer 33 and primer 29. Polymorphic loci (PPL), Nei's diversity index (h), and Shannon index (I) percentages were 6.25, 0.018, and 0.028, respectively. UPGMA analysis separated all isolates based on morphological classification and revealed significant genetic variation among fungal isolates at the intraspecific level. PCoA analysis strongly supported fungal species discrimination and genetic variation. The other parameters of evaluation proved that SCoT markers are at least as effective as other DNA markers. Conclusions: SCoT markers were effective in identifying plant pathogenic fungi and were a powerful tool for estimating genetic variation and population structure of different fungi species.
The Effect of Talaromyces funiculosus ST976 Isolated from Pistacia vera Rhizosphere on Phosphorus Solubility in Soil Samples with Different Physicochemical Properties
(Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 2022) Derviş, Sibel; Türkölmez, Şahimerdan; Eren, Abdullah; Özer, Göksel
In this study, a total of 78 Talaromyces isolates were isolated from the pistachio (Pistacia vera L.) rhizosphere heavily infested with Neoscytalidium spp. The identification studies of the four representative isolates based on morphological and molecular methods showed that all isolates were T. funiculosus. The 575 bp long sequence of the internal transcribed spacer region of T. funiculosus isolate ST976, selected as a representative of the isolates, was deposited in GenBank under accession no. MW130842. The Maximum Likelihood tree clustered the ST976 isolate with reference T. funiculosus isolates derived from the GenBank nucleotide database. The phosphorus dissolution ability of ST976 isolate was determined by an experiment using six soil samples collected from agricultural lands in various locations of Şanlıurfa province. The pH of the soil samples taken varied between 7.21 and 7.88. As a result of the analysis performed with the addition of the isolate ST976 applied to soil samples with different soil structures (Clay and Clay-Loam), it was determined that the isolate ST976 dissolved 109–311% more phosphorus than the control sample. The study is one of the first studies proving the ability of T. funiculosus isolate ST976 to dissolve phosphorus without any additives to soil solution was determined.
Farklı Buğday Genotiplerinden İzole Edilen Endofitik Bakterilerin Fusarium Culmorum'a Karşı Antagonistik Aktivitelerinin Belirlenmesi
(2024) Güney, İnci Güler; Derviş, Sibel; Özer, Göksel; Aktaş, Hüsnü; Keske, Mehmet Ata
Bu araştırmada, farklı buğday genotiplerinden elde edilen endofitik bakterilerin, fizyolojik ve biyokimyasal özelliklerinin ile enzim aktivitelerinin yanısıra buğdayda kök ve kökboğazı çürüklüğüne neden olan Fusarium culmorum’a karşı etkinliğinin değerlendirilmesi amaçlanmıştır. İzolatların F. culmorum’a karşı ikili kültür testlerinden elde edilen sonuçlara göre inhibisyon oranı %80.56- %13.90 arasında değişiklik göstermiştir. In vitro koşullarda Fusarium culmorum’a karşı inhibisyon oranı %80.59 oranıyla Bacillus subtilis (MM11), %69.41 oranıyla Stenotrophomonas maltophilia (EY5) ve %61.10 oranıyla Enterobacter sp. (MY3) en etkili izolatlar olmuştur. Amilaz, selülaz, fosfataz, ACC deaminaz ve siderofor üretiminde Pseudomonas putida (EM9) ve Pseudomonas orientalis (MM21) izolatları tüm testte pozitif sonuç vermiştir. Umut vadeden altı izolat 16S rRNA geninin sekans analizi kullanılarak tanımlanmıştır. Bakteri suşlarının F. culmorum’a karşı etkinliğini değerlendirmek için iklim odası koşullarında (in vivo) saksı denemeleri kurulmuştur. Sonuçlar, Enterobacter sp., S. maltophilia ve B. subtilis (MY3+EY5+MM11) kombinasyonunun hastalık şiddeti, bitki boyu, yaş ağırlık, kuru ağırlık, kök yaş ağırlığı ve kök kuru ağırlığı açısından en uygun sonuçları verdiğini göstermiştir. Stenotrophomonas rhizophila, P. putida ve P. orientalis (EY1+EM9+MM21) kombinasyonu da oldukça etkili olmuştur. Etkili bakteri suşlarının kullanılmasının, çevresel etkilerini en aza indirirken, kimyasal gübre ve pestisitlere olan bağımlılığı ve bunlarla ilişkili maliyetleri azaltması beklenmektedir. Ek doğrulama prosedürlerinin ardından bu suşların, ticari uygulamalar için potansiyelinin olduğu düşünülmektedir. Bulgular, F. culmorum’a karşı çeşitli endofitik bakterilerin kullanıldığı biyolojik kontrol çalışmalarına katkıda bulunacaktır.
Selenyumun Bazı Bitki Patojeni Funguslara Karşı In Vitro Değerlendirmesi
(2024) Özer, Göksel; Türkkan, Muharrem; Sönmez, Ferit; Kabakcı, Hüseyin; Alkan, Mehtap; Derviş, Sibel; Derviş, Sibel
Amaç: Selenyum (Se), tarımsal alanlarda fungal patojenlerini kontrol etmek için umut vaat eden çevre dostu bir element olarak ilgi çekmektedir. Bu çalışmada, sodyum selenit (selenit) ve sodyum selenat (selenat) formlarını içeren Se uygulamalarının 10 farklı bitki patojeni fungus türünün büyümesi üzerindeki etkisi değerlendirilmiştir. Materyal ve Yöntem: Selenyum uygulamalarının fungusların miselyal gelişmesi ve spor oluşumu üzerindeki etkisi in vitro koşullarda değerlendirilmiştir. Probit analizi, fungusların hem miselyal gelişme hem de spor oluşumunda %50 azalmaya (EC50) yol açan tuz konsantrasyonlarını belirlemek için kullanılmıştır. Araştırma Bulguları: En yüksek konsantrasyonda (120 ppm), selenit tüm türlerin miselyal gelişmesini %6.82 ile %62.46 arasında engellemiştir. Buna karşın, selenat daha geniş bir engelleme spektrumu göstermiş ve miselyum büyümesini %0 ile %87.14 arasında etkilemiştir. Farklı konsantrasyonlarda, Fusarium pseudograminearum selenit karşısında (EC50<24 ppm) en yüksek duyarlılığı gösterirken onu Bipolaris sorokiniana ve Verticillium dahliae izlemiştir. Benzer şekilde, Colletotrichum coccodes selenat uygulamasına karşı (EC50<24 ppm) en yüksek duyarlılığı gösterirken, onu B. sorokiniana, Botrytis cinerea, Sclerotinia sclerotiorum ve V. dahliae takip etmiştir. Her iki tuz da anlamlı bir farklılık gözlenmeksizin fungal türleri üzerinde spor oluşumunu etkili bir şekilde inhibe etmiştir. Colletotrichum coccodes, F. pseudograminearum, B. cinerea, F. culmorum, V. dahliae ve B. sorokiniana selenit tarafından anlamlı şekilde inhibe edilmiştir fakat F. oxysporum’a karşı daha düşük bir inhibisyon gözlenmiştir. Benzer şekilde, engelleme yüzdeleri arasında küçük farklar bulunmakla birlikte bu türler, V. dahliae ve F. oxysporum ile birlikte, selenat tarafından anlamlı şekilde inhibe edilmiştir. Colletotrichum coccodes, B. cinerea, F. culmorum, B. sorokiniana ve F. oxysporum için 24 ppm'nin altındaki EC50 değerleri, her iki tuzun da spor oluşumunu etkin bir şekilde inhibe ettiğini göstermiştir. Fusarium pseudograminearum’un %50 inhibisyonu için daha yüksek konsantrasyonların gerektiği anlaşılmıştır. Verticillium dahliae, selenit karşısında 33.16 ppm ve selenat karşısında 24 ppm'nin altında olan EC50 değerleri ile selenata karşı daha yüksek duyarlılık göstermiştir. Sonuç: Bu çalışmanın bulguları, Se'nin sürdürülebilir tarımda çeşitli bitki patojen fungus türleri üzerindeki antifungal potansiyeline ilişkin anlayışımıza katkıda bulunmaktadır. Hastalık yönetimi için elementin mekanizmalarını anlamak ve uygulama protokollerini optimize etmek için daha fazla araştırmaya ihtiyaç vardır.
Citation - WoS: 8
Citation - Scopus: 10
Clonostachys rosea Strain ST1140: An Endophytic Plant-Growth-Promoting Fungus, and Its Potential Use in Seedbeds with Wheat-Grain Substrate
(SpringerLink, 2023) Türkölmez, Şahimerdan; Özer, Göksel; Derviş, Sibel
In this study, Clonostachys rosea strain ST1140, a naturally occurring endophyte in healthy roots of a pistachio tree, was identifed morphologically and molecularly through DNA sequencing, and its endophytic ability and growth efect in/on three solanaceous plant species were tested. Three diferent organic substrates (bread wheat-grain, sawdust, and leonardite) were also investigated for their utility in cultivating and multiplying the strain. In-tray and pot experiments, the rates of seed germination and vegetative development of pepper, tomato, and eggplant inoculated with C. rosea at planting were compared to those of non-inoculated controls. In pot experiments, inoculating seedbeds with increasing doses of C. rosea strain ST1140 with wheat-grain substrate resulted in higher plant height values for all plant species, and the strain endophytically colonized the roots of all plant species. In-tray experiments revealed that ST1140 inoculation resulted in 2–4 days earlier and 10% higher germination rates for all plant species, as well as more vigorous and accelerated seedling growth (10–13 days earlier for seedlings at the 4–5 true leaf stage) on all inoculated plant species. Among three diferent organic substrates, wheat-grain was found to be the most efective for long-term cultivation and multiplication of the fungus, which could be of interest for its development as a commercial product. These results promised the strain ST1140’s use as a biofertilizer in seedbeds with a wheat-grain substrate.
Bipolaris sorokiniana associated with common root rot in Azerbaijan and Kazakhstan
(2022) Alkan, Mehtap; Bozoğlu, Tuğba; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, Göksel
Wheat (Triticum spp.) is the third most important crop in terms of global production, with an average annual production of almost 219 million ha and yielding 760.9 million tons, after maize and rice. However, A complex of fungi attacking the crown and root tissues of wheat causes a serious problem and significant yield reductions in wheat. Although most prevailing species in this complex can change yearly and regionally in dryland winter wheat production areas, Bipolaris sorokiniana Shoemaker (teleomorph: Cochliobolus sativus) is one of the dominant species and causes spot blotch and common root rot (CRR) on wheat plants, especially in winter wheat varieties. Comprehensive surveys were conducted to identify B. sorokiniana associated with CRR of wheat throughout the main wheat-growing areas of Azerbaijan and Kazakhstan in 2017 and 2019, respectively. As a result of the classification of species based on morphological and molecular tools, 96 and 547 isolates were identified as B. sorokiniana for Azerbaijan and Kazakhstan, respectively. All surveyed regions for these countries were contaminated with this pathogen. Some representative isolates of B. sorokiniana were tested for their ability to cause disease and produced moderate disease severities on cultivar Seri 82 (Triticum aestivum, bread wheat). The percent incidence of B. sorokiniana isolates in all isolates was 15.2% for Azerbaijan, whereas, for Kazakhstan, B. sorokiniana was the most frequently recovered species in the three regions surveyed, with an isolated frequency of 44.80%. The results of the current study provide crucial and helpful information to improve disease management strategies against CRR of wheat in these countries.
Citation - WoS: 12
Citation - Scopus: 16
First report of Neoscytalidium dimidiatum associated with dieback and canker of common fig (Ficus carica L.) in Turkey
(SpringerLink, 2022) Güney, İnci Güler; Bozoğlu, Tuğba; Özer, Göksel; Türkölmez, Şahimerdan; Derviş, Sibel
In 2020, a canker disease with dieback of branches and decline of various fig trees in Şanlıurfa and Mardin provinces of Turkey was observed. The causal pathogen was identified as Neoscytalidium dimidiatum based on morphological characteristics and phylogenetic analysis of ITS, tef1, and tub2 loci. Koch’s postulates were confirmed by successful re-isolation of N. dimidiatum only from plants inoculated with the pathogen. This is the first report of N. dimidiatum associated with dieback and canker of common fig in Turkey.
Citation - WoS: 22
Citation - Scopus: 25
Plant-Associated Neoscytalidium dimidiatum-Taxonomy, Host Range, Epidemiology, Virulence, and Management Strategies: A Comprehensive Review
(MDPI, 2023) Derviş, Sibel; Özer, Göksel
Neoscytalidium dimidiatum, a plant- and human-associated fungus, has emerged as a substantial global ecological and agricultural threat aggravated by global warming. It inflicts various diseases, including canker, blight, dieback, leaf spot, root rot, and fruit rot, across a wide spectrum of fruit trees, field crops, shrubs, and arboreal species, with a host range spanning 46 plant families, 84 genera, and 126 species, primarily affecting eudicot angiosperms. Six genera are asymptomatic hosts. Neoscytalidium dimidiatum exhibits worldwide distribution, with the highest prevalence observed in Asia and North America, notably in Iran, Turkey, and California. Rising disease prevalence and severity, aggravated by climate change, particularly impact tropical arid places across 37 countries spanning all 7 continents. This comprehensive review encapsulates recent advancements in the understanding of N. dimidiatum, encompassing alterations in its taxonomic classification, host range, symptoms, geographic distribution, epidemiology, virulence, and strategies for effective management. This study also concentrates on comprehending the taxonomic relationships and intraspecific variations within N. dimidiatum, with a particular emphasis on N. oculus and N. hylocereum, proposing to consider these two species as synonymous with N. dimidiatum. Furthermore, this review identifies prospective research directions aimed at augmenting our fundamental understanding of host-N. dimidiatum interaction.
Citation - WoS: 19
Citation - Scopus: 19
Phytopythium litorale: A novel killer pathogen of plane (Platanus orientalis) causing canker stain and root and collar rot
(Plant Disease, 2020) Derviş, Sibel; Türkölmez, Şahimerdan; Çiftçi, Osman; Özer, Göksel; Serçe, Çiğdem Ulubaş; Dikilitaş, Murat
Decline symptoms associated with lethal stem and branch canker stain along with root and collar rots were observed on 5- to 7-year-old roadside oriental plane trees (Platanus orientalis) in Diyarbakır, Turkey. Above-ground symptoms included leaf necrosis, leaf curling, extensive bluish or blackish staining of shoots, branches, stem bark, and wood surfaces, as well as stem cankers and exfoliation of branch bark scales. A general decline of the trees was distinctly visible from a distance. A Phytophthora/ Pythium-like oomycete species with globose to ovoid, often papillate and internally proliferating sporangia was consistently isolated from the fine and coarse roots and stained branch parts and shoots. The pathogen was identified as Phytopythium litorale based on several morphological features. Partial DNA sequences of three loci, including nuclear rDNA internal transcribed spacer (ITS) and the large ribosomal subunit (LSU), and mitochondrial cytochrome c oxidase subunit II (coxII) confirmed the morphological identification. All P. litorale isolates were homothallic, developing gametangia, ornamented oogonia with elongate to lobate antheridia. Pathogenicity of P. litorale was tested by inoculation on excised shoots and by root inoculation on seedlings. P. litorale produced large lesions and blights on shoots in just 5 days and killed 100% of the seedlings in a month. This paper presents the first confirmed report of P. litorale as an important pathogen on a plant species causing branch and stem cankers, and root and collar rot, in and on P. orientalis, resulting in a rapid decline of trees and suggesting a threat to plane.
Citation - WoS: 5
Citation - Scopus: 6
First report of leaf blight of Turkish oregano (Origanum onites) caused by Neoscytalidium dimidiatum in Turkey
(SpringerLink, 2021) Alkan, Mehtap; Özer, Göksel; Koşar, İslim; Güney, İnci Güler; Derviş, Sibel
In July 2020, a leaf blight disease with intense defoliation on Turkish oregano (Origanum onites L.) plants was observed in a pilot experiment feld in Akçakale and a commercial feld in Haliliye with an incidence of 5.4 and 11.7%, respectively, in Şanlıurfa province. After a surface-sterilization for 1 min with 1% NaOCl, isolations from symptomatic tissues of six afected plants for each feld consistently yielded a fungus with the same colony morphology on potato dextrose agar. Cultures were powdery with a thick aerial mycelium, initially white that changed from olive green to black within 10 days.
Citation - WoS: 5
Citation - Scopus: 7
First report of Neoscytalidium dimidiatum causing foliar and stem blight of lavender in Turkey
(SpringerLink, 2021) Güney, İnci Güler; Özer, Göksel; Turan, İrem; Koşar, İslim; Derviş, Sibel
Lavender (Lavandula angustifolia Mill.) is a valuable medicinal and aromatic plant in Turkey, with a cultivated area of 2,218 hectares in 2020 (TURKSTAT 2021). In June 2020, wilting, extensive stem and leaf blight or necrosis were observed in two-year-old lavender plants cultivated in the experimental felds of the GAP Agricultural Research Institute, Şanlıurfa, Turkey.
Citation - WoS: 5
Citation - Scopus: 6
A novel blight and root rot of chickpea: A new host for Neoscytalidium dimidiatum
(ScienceDirect, 2023) Güney, İnci Güler; Bozoğlu, Tuba; Özer, Göksel; Derviş, Sibel
In the southeastern province of Mardin in Turkey, a severe and novel fungal infection affected all chickpea plant parts, resulting in blight symptoms on stem, petiole, branch, and leaf, defoliation, seed and root rot, and death. Neoscytalidium dimidiatum was identified as the agent responsible for this new blight and root rot using sequence analysis of the ITS, tef1, and tub2 loci as well as morphological data. The pathogen was found in all surveyed districts and fields, with varying incidences of blight and root rot, blight being nearly twice (40%) as common as root rot (21%), and root-rotted and blighted plants co-occurring in the majority of instances. All 92 N. dimidiatum isolates from various tissues induced necrotic lesions on the inoculated plants, indicating they were pathogenic for chickpea. Conidia exhibited germination across a temperature range, with increasing temperatures positively influencing germination rates, and mycelial growth was significantly influenced by temperature, with the optimal growth temperature observed to be 35 °C. The response of 25 Turkish chickpea cultivars and three other genotypes when subjected to inoculation with Ciar 12 and Ciar 78 isolates, representative of phylogenetic clusters, was evaluated based on the severity of blight and root rot. The majority of cultivars and genotypes displayed high susceptibility and suffered mortality when exposed to either soil or spray inoculation with each isolate. Among the assessed cultivars and genotypes, Çağatay had the lowest severity of root rot. This study is the first to report a natural infection of chickpea plants by N. dimidiatum. Under global warming, this may be detrimental to chickpea cultivation and habitat in southeastern Turkey, which is the origin of chickpeas.
Citation - WoS: 1
Citation - Scopus: 2
First report of fruit rot of eggplant caused by Pythium viniferum in Turkey
(SpringerLink, 2021) Türkölmez, Şahimerdan; Özer, Göksel; Çiftçi, Osman; Derviş, Sibel
In August 2019, symptoms including dark brown and irregular sunken lesions or blights on the fruit pedicel and calyx of eggplants (Solanum melongena L.) occurred with a 3% incidence in two felds in Şanlıurfa province of Turkey.