Browsing by Author "Öziç, Cem"

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Evaluation of Antioxidant, Cytotoxicity and Antimicrobial Effects of Achillea Millefolium (YARROW) and Determination of Phytochemicals
(2025) Atalar, Mehmet Nuri; Öziç, Cem; Tel, Ahmet Zafer; Baran, Mehmet Fırat; Arslan, Sevki; Karadağ, Musa; Mensure,
Achillea millefolium (A. millefolium) is one of the medicinal plants growing naturally in Anatolia and widely used in the treatment of many diseases, especially infectious diseases and digestive diseases. In this study, the determination of phenolic compounds, antioxidant, cytotoxic, and antimicrobial effects of plant extract of traditional A. millefolium were investigated. Phenolic compound analysis of the extract of the yarrow plant obtained with methanol was carried out by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC–ESI–MS/MS). Antimicrobial effect was determined by the minimum inhibitory concentration (MIC) method and cytotoxicity analyses were carried out on androgen-dependent prostate cancer cells (LNCaP), human embryonic kidney 293 cancer cell lines (HEK293), and colorectal adenocarcinoma (CaCO-2) cell lines by the MTT method. Total antioxidant capacity was determined by the DPPH free radical scavenging activity determination method. In the determination of antimicrobial activity, it was tested on gram-positive Bacillus subtilis (B. subtilis.), gram-negative Escherichia coli (E.Coli) and Candida albicans (C. albicans) fungi. Among total phenolics, chlorogenic acid, caffeic acid, and salicylic acid were detected in high amounts. In the tests, the highest cytotoxic effect was shown on the LNCaP cell line, and the highest antimicrobial effect was shown on B. subtilis.
In Vitro Antioxidant, Antimicrobial, Anticholinesterase Activities, and Phenolic and Heavy Metal Compositions of Prunus Avium Fruit Extracts
(2025) Öziç, Cem; Baran, Mehmet Fırat; Baran, Ayşe; Bıçakcıoğlu, Işınsu; Aras, İbrahim Oğuz
Prunus avium L. (P. avium) is one of the most admired fruits for its organoleptic and nutritional value. This research examined cherry fruit chloroform and methanol extracts antioxidant, anticholinesterase, antibacterial, and chemical and elemental abilities. The extracts antioxidant capacity was assessed by evaluating total phenolic and flavonoid content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. P. avium fruit chloroform (Chl) extract had the greatest phenolic and flavonoid content. However, the methanol (Me-OH) extract exhibited stronger radical-scavenging activity. The extracts antibacterial activity was determined using the MIC method, and the enzyme inhibition activity was ascertained using a spectroscopic technique. Both extracts inhibited Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Candida albicans (C. albicans) growth at low concentrations better than standard antibiotics. The phenolic chemicals that could be causing the biological activity in the extracts were identified using LC-MS/MS. Major components in methanol extract were cichoric acid, chlorogenic acid, and citric acid, while in chloroform extract vanillic acid, cichoric acid, and chlorogenic acid. Additionally, the presence of zinc, copper, mercury, iron, nickel, arsenic, cadmium, lead, and selenium in P. avium fruits was investigated by ICP-OES Optical Emission Spectrometry. Mercury, nickel, and selenium weren't detected in the sample, while arsenic was beyond permitted levels. The fruit of P. avium may have potential biological and industrial applications owing to its rich biochemical and elemental composition and its antioxidant, antibacterial, and anticholinesterase capabilities.