Browsing by Author "Öziç, Cem"

Now showing 1 - 3 of 3

Evaluation of Antioxidant, Cytotoxicity and Antimicrobial Effects of Achillea Millefolium (YARROW) and Determination of Phytochemicals
(2025) Atalar, Mehmet Nuri; Öziç, Cem; Tel, Ahmet Zafer; Baran, Mehmet Fırat; Arslan, Sevki; Karadağ, Musa; Mensure,
Achillea millefolium (A. millefolium) is one of the medicinal plants growing naturally in Anatolia and widely used in the treatment of many diseases, especially infectious diseases and digestive diseases. In this study, the determination of phenolic compounds, antioxidant, cytotoxic, and antimicrobial effects of plant extract of traditional A. millefolium were investigated. Phenolic compound analysis of the extract of the yarrow plant obtained with methanol was carried out by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC–ESI–MS/MS). Antimicrobial effect was determined by the minimum inhibitory concentration (MIC) method and cytotoxicity analyses were carried out on androgen-dependent prostate cancer cells (LNCaP), human embryonic kidney 293 cancer cell lines (HEK293), and colorectal adenocarcinoma (CaCO-2) cell lines by the MTT method. Total antioxidant capacity was determined by the DPPH free radical scavenging activity determination method. In the determination of antimicrobial activity, it was tested on gram-positive Bacillus subtilis (B. subtilis.), gram-negative Escherichia coli (E.Coli) and Candida albicans (C. albicans) fungi. Among total phenolics, chlorogenic acid, caffeic acid, and salicylic acid were detected in high amounts. In the tests, the highest cytotoxic effect was shown on the LNCaP cell line, and the highest antimicrobial effect was shown on B. subtilis.
In Vitro Antioxidant, Antimicrobial, Anticholinesterase Activities, and Phenolic and Heavy Metal Compositions of Prunus Avium Fruit Extracts
(2025) Öziç, Cem; Baran, Mehmet Fırat; Baran, Ayşe; Bıçakcıoğlu, Işınsu; Aras, İbrahim Oğuz
Prunus avium L. (P. avium) is one of the most admired fruits for its organoleptic and nutritional value. This research examined cherry fruit chloroform and methanol extracts antioxidant, anticholinesterase, antibacterial, and chemical and elemental abilities. The extracts antioxidant capacity was assessed by evaluating total phenolic and flavonoid content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. P. avium fruit chloroform (Chl) extract had the greatest phenolic and flavonoid content. However, the methanol (Me-OH) extract exhibited stronger radical-scavenging activity. The extracts antibacterial activity was determined using the MIC method, and the enzyme inhibition activity was ascertained using a spectroscopic technique. Both extracts inhibited Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Candida albicans (C. albicans) growth at low concentrations better than standard antibiotics. The phenolic chemicals that could be causing the biological activity in the extracts were identified using LC-MS/MS. Major components in methanol extract were cichoric acid, chlorogenic acid, and citric acid, while in chloroform extract vanillic acid, cichoric acid, and chlorogenic acid. Additionally, the presence of zinc, copper, mercury, iron, nickel, arsenic, cadmium, lead, and selenium in P. avium fruits was investigated by ICP-OES Optical Emission Spectrometry. Mercury, nickel, and selenium weren't detected in the sample, while arsenic was beyond permitted levels. The fruit of P. avium may have potential biological and industrial applications owing to its rich biochemical and elemental composition and its antioxidant, antibacterial, and anticholinesterase capabilities.
Investigation of the Effect of Cuscuta Spp. Extract on Cell Viability
(2025) Öziç, Cem; Baran, Ayşe; Aktepe, Necmettin; Güneş, Zübeyir; Baran, Mehmet Firat
Cancer is becoming an increasingly serious health problem worldwide. While various treatment approaches exist for diseases like colorectal cancer, they have not yet been found to be fully effective. Therefore, the development of new anti-cancer drugs is crucial. Cuscuta is a plant used in functional foods and for traditional medicinal purposes in various cultures, including Ayurveda and its medicinal traditions, which are widespread throughout the world. In preclinical studies, Cuscuta spp. have demonstrated anticytotoxic and cytoprotective properties and potential anticancer effects, particularly in various cell lines and animal models. Fibroblasts are scientifically proven to be the primary normal cell type of connective tissue, typically mesenchymal in origin. This is represented by the laboratory L929 mouse fibroblast cell line. Colorectal cancer is the third most common cancer worldwide and the third most common cause of cancer-related death. Our study aimed to determine the cytotoxic effects of Cuscuta spp extract, known to have medicinal effects in various diseases in public health, on the L929 mouse fibroblast cell line and the HT-29 human colorectal adenocarcinoma cell line. For cytotoxic analysis, the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] enzymatic test was preferred. In vitro testing using Cuscuta spp extract revealed decreased viability in L929 fibroblast cell line and HT-29 human colorectal adenocarcinoma cells exposed to Cuscuta spp plant extract for 24 and 48 hours. The plant extract showed more cytotoxic effects on fibroblast cells, a normal cell line. In conclusion, this study demonstrates that Cuscuta spp. extract exhibits high cytotoxicity on normal cells and has limited effects on cancer cells. Further studies are needed for its pharmacological use.