Browsing by Author "Ercan, Leyla"

Now showing 1 - 7 of 7

Antioxidant and Antimicrobial Capacity of Quinic Acid
(Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 2022) Ercan, Leyla; Doğru, Mehmet
Recently, agents with natural antioxidant and antimicrobial properties have been popularly studied. For this purpose, phenolic compounds, terpenes, and organic acids are examined for their antioxidant and antimicrobial properties. Of these, organic acids are increasingly being used in pharmacology, medicine, food, and industry. Quinic acid is a natural organic compound found in many edible fruits and plants. In this study, the antioxidant effect of quinic acid, which has the structure of cyclohexane carboxylic acid, was determined in vitro using seven different methods (DPPH, ABTS, CUPRAC, DMPD, FRAP, Fe3+ reduction, and Total antioxidant method). In addition, its antimicrobial effect on fungi (C. albicans), gram-positive bacteria (S. aureus, S. pyogenes), and gram-negative bacteria (E. coli, K. pneumoniae, and P. aeruginosa) were determined by the disk diffusion method. As a result, it was found that quinic acid has broad-spectrum antimicrobial properties, but its antioxidant properties are too low to be highlighted. While its antimicrobial activity was quite good, especially on K. pneumoniae E. coli, S. aureus, S. Pyogenes, and P. aeruginosa, it did not show any effect on C. albicans. Although the antioxidant property of quinic acid is low, it showed more antioxidant properties in the DMPD method, which is one of these methods, because it dissolves very well in water.
Citation - WoS: 0
Comparison of Antioxidant and Antimicrobial Properties of Zinc Oxide and Selenium Oxide Nanoparticles Using Verbascum Kotschyi Boiss. & Hohen
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2025) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Kilic, Fatma M. U. N. G. A. N.; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Nanoparticle applications have been studied in many fields in recent years. Among these studies, the synthesis of nature-friendly and healthfriendly nanoparticles through green synthesis attracts much attention. These investigations also highlight the significance of several plant species, many of whose worth and traits remain unknown. The goal of this work is to create zinc oxide and selenium nanoparticles from Verbascum kotschyi Boiss. & Hohen., a plant species that hasn't received much attention, and to ascertain the antioxidant and antibacterial qualities of these nanoparticles. To accomplish this, three distinct techniques (DPPH, CUPRAC, and FRAP) were used to assess the produced nanoparticles' in vitro antioxidant capabilities after SEM, EDX, and FTIR analyses. Furthermore, the disk diffusion technique was utilized to ascertain the antibacterial efficacy of these nanoparticles against both gram-positive and gram-negative bacteria and fungus. In conclusion, V. kotschyi-derived zinc oxide nanoparticles outperformed selenium nanoparticles in terms of antibacterial activity. But when it came to antioxidant activity, selenium nanoparticles outperformed zinc oxide nanoparticles. Thus, it was determined that the products created by nanoparticle synthesis from Verbascum kotschyi have properties that can be used in different fields.
Citation - WoS: 3
Citation - Scopus: 2
Comparison of chemical and antimicrobial properties of different nanoparticles synthesized from Verbascum x calcicolum Hub.-Mor. Hybrid
(Elsevier, 2024) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Kilic, Murat; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Recent advances in nanoparticle synthesis have led to an increase in the use of environmentally acceptable plantderived nanoparticles, given their wide range of applications. Due to their stability and several advantageous characteristics, selenium nanoparticles also stand out in these investigations in addition to metallic nanoparticles like silver, gold, iron, and zinc. These nanoparticles' favorable qualities for human health, such as antioxidant and antibacterial activities, are being studied in addition to their chemical characteristics. This investigation aimed to produce zinc oxide nanoparticles (VcZnO) and selenium nanoparticles (VcSe) from hybrid Verbascum x calcicolum Hub.-Mor. And to examine their antioxidant and antimicrobial properties. Utilizing the disk diffusion method and well methods, its antibacterial capabilities were studied. In vitro antioxidant capabilities were also assessed utilizing the DPPH, FRAP, and CUPRAC techniques. As a result, it emerged that zinc oxide nanoparticles synthesized from hybrid Verbascum x calcicolum were effective on gram-positive bacteria and gram-negative bacteria such as Staphylococcus aureus (MIC = 160 mg/ml for VcZnO), Pseudomonas aeruginosa (MIC = 80 mg/ml for VcZnO), Klebsiella pneumoniae (MIC = 40 mg/ml for VcZnO and, MIC = 120 mg/ml for VcSe), Escherichia coli (MIC = 20 mg/ml for VcZnO). While selenium nanoparticles are more favorable in terms of antioxidant capabilities, zinc nanoparticles demonstrate stronger antibacterial properties than selenium nanoparticles. Thus, it has been discovered that the use of different nanoparticles of various plants can be more efficient in different applications. It has also been found that Verbascum x calcicolum is a material that can be used successfully in nanoparticle synthesis.
Citation - WoS: 3
Determination of Antimicrobial Activity of Nasturtium Officinale and Its Content of Volatile Organic Compounds and Fatty Acids
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Ercan, Leyla; Dogru, Mehmet
Due to the side effects of antibiotics used in the treatment of diseases caused by pathogenic bacteria, and antibiotic resistance that develops due to the misuse of antibiotics, scientists have turned to the search for alternative antimicrobial compounds. Plants and antimicrobial compounds in plants are widely researched because they are natural and have been a familiar resource in the field of complementary medicine for centuries. In this study, antimicrobial activities of the methanol and water extracts of Nasturtium officinale prepared at different concentrations were investigated on gram-positive bacteria, gram-negative bacteria, and fungi by the disc diffusion method. In addition, volatile organic compound and fatty acidcontent of the plant were determined. For this purpose, fatty acids were determined by converting them to methyl esters in GC-FID (gas chromatography flame ionization detector), volatile compounds were determined by SPME (Solid-phase microextraction) method in GC-MS (gas chromatography-mass spectrometry). In addition, the amounts of volatile components in different parts of the plant were shown comparatively within the scope of the research. According to the results obtained; it was revealed that Nasturtium officinale has an antimicrobial effect on Bacillus megaterium, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Bacillus Spizizenii, Klebsiella pneumoniae, Staphylococcus aureus bacteria. The plant showed a stronger antimicrobial effect, especially on P. aeruginosa, C. Albicans, and E. coli. It has also been determined that Nasturtium officinale has important essential fatty acids as well as many volatile components. In the analyzes made, it was determined that the main volatile component of Nasturtium officinale was alpha-Terpinolene.
Determination of Phenolic Compounds in Nasturtium Officinale by Lc-Ms / Ms Using Different Extraction Methods and Different Solvents
(2023) Ercan, Leyla; Doğru, Mehmet
This study aimed to determine the phenolic compound contents of Nasturtium officinale (N. officinale) extracts prepared with water, methanol, ethanol, and chloroform, as well as extracts prepared by evaporation and lyophilization, and parts of N. officinale by LC-MS/MS (Liquid Chromatography-Mass spectrometry/Mass spectrometry). The results obtained not only contribute to knowledge about the benefits of N. officinale but also show the effect of different solvents and different extraction methods on the determination of phenolic compounds. In addition to these results, the importance of the cold chain in the determination of some phenolic compounds has also been demonstrated. While it is observed that the number of phenolic components obtained from the extracts prepared with methanol and ethanol is high; It is observed that some phenolic compounds such as quinic acid, fumaric acid, aconitic acid, and p-coumaric acid dissolve very well in water and temperature is important.
Citation - WoS: 2
Citation - Scopus: 1
Investigation of Antibacterial and Antifungal Efficacy of Zinc and Silver Nanoparticles Synthesized From Nasturtium Officinale
(Ankara Univ, Fac Agriculture, 2023) Ercan, Leyla
Nanoparticles are nano -sized materials that can be widely used in fields such as medicine, pharmacology, and industry. The use of natural and easily available materials in nanoparticle synthesis is preferable for economic reasons. Plants are extremely suitable for the synthesis of nanoparticles due to their wide availability and the large number of components they contain with various properties. For this purpose, silver nanoparticles and zinc nanoparticles (AgNPs and ZnNPs), two different nanoparticles were synthesized from an edible plant, watercress (Nasturtium officinale). Scanning electron microscopy, scanning electron microscopy -energy dispersive X-ray, UV-VIS spectroscopy, X-ray diffraction (XRD), and fourier transform infrared spectrophotometer (FTIR) analyses of these nanoparticles were performed. In addition, the antimicrobial effects of these synthesized nanoparticles were determined using the disk diffusion method. The nanoparticles obtained from Nasturtium officinale were effective on Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa), Gram-positive bacteria (Staphylococcus aureus, Streptococcus pyogenes), and fungi (Candida albicans). In particular, AgNPs with broad-spectrum antimicrobial activity were obtained from the watercress. While ZnNPs showed inhibition effects of 49% on K. pneumoniae, 51% on S. aureus, and 62% on C. albicans, AgNPs showed inhibition effects of 93% on P. aeruginosa, 87% on S. aureus, 81% on E. coli, 80% on C. albicans, 72% on K. pneumoniae, and 56% on S. pyogenes. The results show that Nasturtium officinale can be used effectively in the production of new biotechnological products, particularly ones with antimicrobial properties.
Citation - WoS: 0
Citation - Scopus: 0
Phytochemical Profile, Antimicrobial, Antioxidant, Anti-Xanthine Oxidase, and Anti-Elastase Activities of Centaurea Hyalolepis: an in Silico and in Vitro Analysis
(Wiley-v C H verlag Gmbh, 2025) Günbegi Çalışkan, Cemile; Caliskan, Cemile Gunbegi; Akan, Hasan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
This study aimed to examine the antibacterial, antioxidant, and enzyme activity of Centaurea hyalolepis, as well as the total phenolic and carotenoid contents, and volatile and phenolic components. Gas chromatography-mass spectrometry was used for the volatile component analysis of C. hyalolepis, while liquid chromatography-mass spectrometry was used for the phenolic component analysis. Furthermore, the antimicrobial activity was evaluated using disc diffusion and microdilution techniques. The antioxidant capacity was investigated in vitro using four distinct methods (2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, cupric ion-reducing antioxidant capacity, and N,N-dimethyl-p-phenylene diamine). Additionally, both in vitro and in silico studies were carried out to determine the inhibitory effect on xanthine oxidase and elastase. Consequently, it was determined that C. hyalolepis has antioxidant properties and is a highly efficient antibacterial agent against Bacillus megaterium, Pseudomonas aeruginosa, Bacillus subtilis subsp. spizizenii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, and Candida albicans. Additionally, C. hyalolepis contains many volatile compounds, such as 2-methyl propanoic acid, 3,5-octadien-2-one, 2,6-dimethyl cyclohexanol, hexanal, pentanal, anethole, and beta-cyclocitral. It also has a large number of phenolic components, including chlorogenic acid, apigenin 7-glucuronide, quinic acid, and 4-hydroxybenzoic acid. Moreover, C. hyalolepis was shown to inhibit xanthine oxidase and elastase.