Browsing by Author "Ipek, Polat"

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Citation - WoS: 2
Citation - Scopus: 2
Antioxidant Properties of allium Turcicum Özhatay & Cowley Plant Extract, Its Effects on the Proliferation and Migration of Cancer Cells
(Frontiers Media Sa, 2024) Ipek, Polat; Baran, Ayse; Cebe, Deniz Baris; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Firat
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum & Ouml;zhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum & Ouml;zhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 mu g/mL, respectively. We conducted a cell migration experiment to study the A. turcicum & Ouml;zhatay & Cowley Extract (AT & Ouml;CE) impact on cancer cells' metastatic behavior. Our findings indicate that AT & Ouml;CE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of AT & Ouml;CE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 +/- 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 +/- 0.044 mg GAE/g.
Citation - WoS: 2
Citation - Scopus: 2
Deciphering the Effect Of Potentilla Fulgens Root Extract Against Healthy Huvec Cell Line and Cancer Cell Lines (a549 and Skov-3)
(Elsevier, 2024) Ipek, Polat; Baran, Ayse; Baran, Mehmet Firat; Eftekhari, Aziz; Khusro, Ameer; Ommati, Mohammad Mehdi; Almutairi, Saeedah Musaed; Fırat Baran, Mehmet; Mehdi Ommati, Mohammad; Musaed Almutairi, Saeedah
Background: Potentilla fulgens, , a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose- dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) P <0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-kappa B after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
Citation - WoS: 8
Citation - Scopus: 10
Determination of Chemical Components of the Endemic Species allium Turcicum L. Plant Extract by Lc-ms/Ms and Evaluation of Medicinal Potentials
(Cell Press, 2024) Ipek, Polat; Atalar, Mehmet Nuri; Baran, Ayse; Baran, Mehmet Firat; Ommati, Mohammad Mehdi; Karadag, Musa; Khalilov, Rovshan
The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU -145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 mu g/mL and 2000 mu g/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU -145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU -145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00 - 8.00 mu g/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies.
Citation - WoS: 19
Citation - Scopus: 20
Green synthesis and characterization of selenium nanoparticles (Se NPs) from the skin (testa) of Pistacia vera L. (Siirt pistachio) and investigation of antimicrobial and anticancer potentials
(SpringerLink, 2023) Keskin, Cumali; Mehmet Fırat Baran, Cumali Keskin, Ayşe Baran, Kadri Kurt, Polat İpek, Aziz Eftekhari, Rovshan Khalilov, Ismayil Fridunbayov, William C. Cho; Baran, Mehmet Firat; Eftekhari, Aziz; Kurt, Kadri; Baran, Ayse; Ipek, Polat; Cho, William C.
Metallic nanoparticles created by ecologically friendly synthesis processes are becoming increasingly useful in a variety of applications. Because of their strong bioactive component qualities, biocompatible architectures, high stability, and low toxicity, green-produced selenium nanoparticles are particularly signifcant materials for various medicinal applications. Plants include a wealth of essential phytochemicals with therapeutic and medical capabilities. Pistachio vera L. (Siirt pistachio) is a seasonal fruit that is frequently consumed for its nutritional worth and health advantages. However, the outer colored skin of the P. vera (Siirt pistachio) fruit, which is not consumed, contains many biologically active compounds. In this study, plant-mediated synthesis of selenium nanoparticles (SeNPs) was successfully accomplished after adding the sodium selenite solution to the aqueous extract of P. vera colored skin waste. The synthesized Se NPs were characterized with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta distribution, atomic force microscope (AFM), Fourier transform infrared spectrum (FTIR), thermogravimetric (TGA) and diferential thermal analysis, X-ray difraction (XRD), and energy-dispersive X-ray spectrum (EDX). Biogenic Pistacia vera (PV) PV-SeNPs were also tested for their ability to suppress the development of various pathogenic bacteria and cancerous cell lines. The UV-visible (UV–Vis) spectra revealed an absorption peak at 325 nm, which refected the surface plasmon band. The signifcant selenium signal on the EDX spectrum at 1.5 keV confrmed the creation of Se NPs. The presence of several peaks on the FTIR spectrum of the aqueous extract of Pv and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols, which are important in facilitating the process of capping and bioreduction, as well as conferring stability to nanoparticles. The TEM microphotographs revealed that the nanoparticles were highly distributed, had a spherical morphological form, and were monodisperse below 10 nm. Biogenic Pv-SeNPs exhibited similar antimicrobial activity as standard antibiotics. However, it was determined that the cytotoxic activity of Se-NPs against cancer cell lines was quite high depending on the dose and time. As a result, the Pv-SeNPs are likely to be extremely benefcial in the pharmaceutical and cosmetic sectors, as well as in the food and cosmetic industries, in producing antimicrobial and/or anticancer medications.
Citation - WoS: 56
Citation - Scopus: 62
Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract
(SpringerLink, 2023) Baran, Ayşe; Polat İpek, Mehmet Fırat Baran, Ayşe Baran, Abdulkerim Hatipoğlu, Cumali Keskin, Mahmut Yildiztekin, Selçuk Küçükaydin, Hatice Becerekli, Kadri Kurt, Aziz Eftekhari, Irada Huseynova, Rovshan Khalilov, William C. Cho; Baran, Mehmet Firat; Yildiztekin, Mahmut; Keskin, Cumali; Hatipoglu, Abdulkerim; Ipek, Polat; Cho, William C.
Gold nanoparticles (Au NPs) have potentially therapeutic properties as they are synthesized via biomolecules as reducing and stabilizing agent(s). The aim of this study is to develop an easy and eco-friendly method for the synthesis of Au NPs using extracts from the Allium cepa (AC) red peel (skin) extract and investigate the antibacterial and antioxidant activity and also inhibitory efects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. UV–Vis peak at~564 nm confrmed the Au NPs absorbance. TEM images revealed the formation of Au NPs with mostly spherical shapes and sizes between 6.08 and 54.20 nm. FTIR analysis confrmed the important biological compounds responsible for the reduction of gold. The strong absorption property of Au NPs was studied by EDX. The produced Au NPs demonstrated signifcant antibacterial and antifungal activity against the bacterial and fungal strains tested, as well as efcient inhibitory activity against both AChE and BChE enzymes. The highest antimicrobial activities were found against Staphylococcus aureus (0.06° mg/ ml) and Candida albicans (0.06° mg/ml). The antioxidant test fndings revealed that AC-Au NPs had lesser activity when compared to normal antioxidants. The Au NPs showed excellent inhibitory efcacy against AChE and BChE. The proposed green technique could encourage the innocuous generation of Au NPs, implying therapeutic possibilities.
Green Synthesis of Silver Nanoparticles Derived from Papaver Rhoeas L. Leaf Extract: Cytotoxic and Antimicrobial Properties
(MDPI, 2023) Baran, Mehmet Firat; Beylerli, Ozal; Baran, Ayse; Sufianov, Albert; Yildiz, Resit; Hatipoglu, Abdulkerim; Ipek, Polat
In the last few decades, the search for metal nanoparticles as an alternative to cancer treatments and antibiotics has increased. In this article, the spectroscopic (ultraviolet-visible (UV-vis), electron-dispersing X-ray (EDX), and Fourier transform infrared (FT-IR)), microscopic (field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), and atomic force microscope (AFM)), structural (X-ray diffractometer (XRD) and zetasizer), and analytic (thermogravimetric/differential thermal analyzer (TGA-DTA)) characterization of the silver nanoparticles (AgNPs) produced from Papaver rhoeas (PR) L. leaf extract are presented. PR-AgNPs are generally spherical and have a maximum surface plasmon resonance of 464.03 nm. The dimensions of the manufactured nanomaterial are in the range of 1.47-7.31 nm. PR-AgNPs have high thermal stability and a zeta potential of 36.1 mV. The minimum inhibitory concentration (MIC) values (mg L-1) of PR-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans are 1.50, 0.75, 3.00, 6.00, and 0.37, respectively. In the study, the cytotoxic and proliferative effects of PR-AgNPs using the MTT (3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide) method on various cancer cell lines (CACO-2 (human colon adenocarcinoma cell), MCF-7 (human breast cancer cell), T98-G (glioblastoma multiforme cell), and healthy HUVEC (human umbilical vein endothelial cell)) cell lines are presented. After 24 and 48 h of the application, the half-maximum inhibitory concentration (IC50) values (mu g mL(-1)) of PR-AgNPs on HUVEC, CACO-2, MCF-7, and T98-G lines are 2.365 and 2.380; 2.526 and 2.521; 3.274 and 3.318; 3.472 and 3.526, respectively. Comprehensive in vivo research of PR-AgNPs is proposed to reveal their potential for usage in sectors such as nanomedicine and nanochemistry.
Potentilla Fulgens Root Extract’s Effect on Breast Cancer (mcf-7) and Osteosarcoma (u2os) Cells Proliferation and Migration
(2024) Ipek, Polat; Baran, Ayse
Aim: This study evaluates the cytotoxic effect of Potentilla fulgens root extract on MCF7, U2OS, and RPE-1 cell lines and investigates its impact on cell migration. Materials and Methods: The effect of Potentilla fulgens root extract on the cell viability of MCF-7, U2OS, and RPE-1 cells was analyzed using an MTT assay. The effect of the compounds on cell migration was evaluated at 24 and 48 hours using the wound healing assay. A wound-healing assay was used to measure the metastatic ability of cells in vitro. Results: MTT assay showed that PRE had a cytotoxic effect on all three cell lines, depending on dose and time, and this effect was statistically significant. The wound healing assay results showed that PRE slowed the migration of live MCF-7 and U2OS cancer cells, and these effects increased over time. Conclusion: As a result, this study indicates that PRE may be very useful in treating human osteosarcoma and breast cancer.