Browsing by Author "Koc, Murat"

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Citation - WoS: 5
Citation - Scopus: 5
Medicinal Evaluation and Molecular Docking Study of Osajin as an Anti-Inflammatory, Antioxidant, and Antiapoptotic Agent Against Sepsis-Associated Acute Kidney Injury in Rats
(Taylor & Francis Ltd, 2024) Alhilal, Mohammad; Erol, Huseyin Serkan; Yildirim, Serkan; Cakir, Ahmet; Koc, Murat; Alhilal, Suzan; Halici, Mesut Bunyami
Despite efforts to find effective drugs for sepsis-associated acute kidney injury (SA-AKI), mortality rates in patients with SA-AKI have not decreased. Our study evaluated the protective effects of isoflavone osajin (OSJ) on SA-AKI in rats by targeting inflammation, oxidative stress, and apoptosis, which represent the cornerstones in the pathophysiological mechanism of SA-AKI. Polymicrobial sepsis was induced in rats via the cecal ligation and puncture (CLP) technique. Markers of oxidative stress were evaluated in kidney tissues using biochemical methods. The expression of interleukin-33 (IL-33), 8-hydroxydeoxyguanosine (8-OHdG), caspase-3, and kidney injury molecule-1 (KIM-1) was evaluated as indicators of inflammation, DNA damage, apoptosis, and SA-AKI respectively in the kidney tissues using immunohistochemical and immunofluorescent detection methods. The CLP technique significantly (p < 0.001) increased lipid peroxidation (LPO) levels and significantly (p < 0.001) decreased the activities of superoxide dismutase and catalase in kidney tissues. In the renal tissues, strong expression of IL-33, 8-OHdG, caspase-3, and KIM-1 was observed with severe degeneration and necrosis in the tubular epithelium and intense interstitial nephritis. In contrast, the administration of OSJ significantly (p < 0.001) reduced the level of LPO, markedly improved biomarkers of antioxidant status, decreased the levels of serum creatinine and urea, lowered the expression of IL-33, 8-OHdG, caspase-3, and KIM-1 and alleviated changes in renal histopathology. A promising binding score was found via a molecular docking investigation of the OSJ-binding mode with mouse IL-33 (PDB Code: 5VI4). Therefore, OSJ protects against SA-AKI by suppressing the IL-33/LPO/8-OHdG/caspase-3 pathway and improving the antioxidant system.
Citation - WoS: 10
Citation - Scopus: 9
Osajin from Maclura pomifera alleviates sepsis-induced liver injury in rats: biochemical, histopathological and immunohistochemical estimation
(Taylor & Francis Online, 2023) Alhilal, Mohammad; Huseyin Serkan Erol, Serkan Yildirim, Ahmet Cakir, Murat Koc, Demet Celebi, Mesut Bunyami Halici; Koc, Murat; Cakir, Ahmet; Erol, Huseyin Serkan; Celebi, Demet; Yildirim, Serkan; Halici, Mesut Bunyami
This paper aimed to examine the impact of flavonoid osajin (OSJ) on liver damage induced by sepsis. A total of 30 male rats were divided into 5 groups (Sham, sepsis, OSJ 150, OSJ 300 and reference). During sepsis, elevated lipid peroxidation (LPO) level and catalase activity (CAT) and decreased glutathione (GSH) level and superoxide dismutase (SOD) activity were observed in hepatic tissues of sepsis group in comparison with Sham group. A strong interleukin-33 and caspase-3 expressions were detected in hepatic tissues of sepsis group. On the contrary, OSJ administration to OSJ 300 group showed a significant decrease (P < 0.001) in LPO level (176±2.926) and significant increase (P < 0.001) in GSH level (10.586±0.083) and SOD activity (29.152±0.094) in comparison with sepsis group (185.777±1.735, 8.246±0.124, 24.307±0.379 respectively). In addition, the consumption of OSJ reduced expressions of interleukin-33 and caspase-3 and improved histopathological integrity. In conclusions, OSJ has hepatoprotective effect against sepsis-induced liver injury.
Pomiferin Protects against Sepsis-Associated Acute Liver and Kidney Injury via Inhibition of NF-κB Activation, Oxidative Stress, and Cytochrome-c
(Nature Portfolio, 2026) Koc, Murat; Alhilal, Mohammad; Erol, Huseyin Serkan; Alhilal, Suzan; Dereli, Esra; Yildirim, Serkan; Halici, Mesut Bunyami
In this study we report the first identification of the therapeutic effects of pomiferin isolated from Maclura pomifera against acute liver and kidney injury induced by sepsis. These results were obtained using a rat model of sepsis. We focused on targeting the nuclear factor kappa B (NF-kappa B) activation cascade, oxidative stress, and cytochrome-c, three key components involved in the pathophysiology of sepsis-associated acute liver and kidney injury. This assessment was conducted using biochemical, histopathological, immunohistochemical, and immunofluorescence analyses to examine parameters in liver and kidney tissues. The cecal ligation and puncture technique, used to induce sepsis, consistently caused acute liver and kidney damage. This was evidenced by significant increases (p < 0.0001), relative to untreated control rats, in the abundance of Toll-like receptor 4, NF-kappa B p65, phospho-NF-kappa B p65, 8-hydroxydeoxyguanosine, cytochrome-c, and caspase-3, higher degeneration, lipid peroxidation, and necrosis. This technique also caused significant decreases (p < 0.001) in components of the cellular antioxidant system in the hepatic and renal tissues of septic rats. Pomiferin, particularly at a dose of 300 mg/kg, showed promising pharmacological effects by reversing these pathological changes. Overall, pomiferin appears to protect liver and kidney tissues during sepsis by suppressing the NF-kappa B activation cascade, reducing oxidative stress, and lowering cytochrome-c activity. These effects suggest that pomiferin may be useful for managing sepsis patients with acute liver and kidney injury.