Browsing by Author "Mermutluoglu, Cigdem"

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Investigation of Anti-Neutrophil Cytoplasmic Antibody by Indirect Immunofluorescence Assay in COVID-19 Patients
(Galenos Publ House, 2025) Ayaydin, Zeynep; Ozcan, Nida; Mermutluoglu, Cigdem; Atmaca, Selahattin; Tekin, Ali Cem
Aim: Coronavirus disease-2019 (COVID-19) has been suggested to trigger the production of autoimmune antibodies and contribute to the development of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aims to investigate the presence of ANCA among COVID-19 patients. Methods: This cross-sectional, prospective analysis included 200 COVID-19 patients with positive polymerase chain reaction test results for severe acute respiratory syndrome-coronavirus-2 and no history of autoimmune disease, recruited between June 2021 and November 2022. The control group included 50 age-matched healthy blood donors. The ANCA profile was assessed using the indirect immunofluorescence assay method with the EUROPLUS Granulocyte Mosaic EUROPattern test kit (EUROIMMUN, Germany) on sera samples of the patient and control groups. Results: Perinuclear ANCA (p-ANCA) was detected in 12 of 200 COVID-19 patients (6.0%) and cytoplasmic ANCA (c-ANCA) was detected in 15 of 200 patients (7.5%). No ANCA positivity was observed in the control group (0/50). ANCA positivity among COVID-19 patients (27 of 200, 13.5%) was statistically significantly higher than in the control group (p<0.05). ANCA positivity was significantly higher in intensive care unit (ICU) patients (21 of 77, 27.3%) compared to non-ICU patients (6 of 123, 4.9%) (p<0.05). Conclusion: ANCA presence in ICU patients supports the hypothesis that COVID-19 triggers ANCA synthesis and contributes to disease severity.
Role of vitamin D, folic acid, ferritin, inflammation and oxidative stress in the pathogenesis of COVID-19
(Progress in Nutrition, 2022) Unsal, Velid; Ilhan Sabancilar, Erdal Ozbek, Cigdem Mermutluoglu, Hakan Temiz; Ozbek, Erdal; Mermutluoglu, Cigdem; Temiz, Hakan; Sabancilar, Ilhan
Abstract. The COVID-19 pandemic is one of the most devastating and significant events of recent times. COVID-19 has so far become one of the worst infectious disease outbreaks of recent times, with more than 635 million cases and more than 6.6 million deaths. Viruses cause an explosion of inflammatory cytokines and reactive oxygen types. Oxidative stress is thought to have a key role in COVID-19. vitamin D, folic acid, calcium (Ca), magnesium (Mg) and ferritin levels are thought to be associated with COVID-19. This study aims to investigate the role of oxidative stress, inflammation, vitamin D and folic acid, ferritin, Ca and Mg in the pathogenesis of COVID-19. Materials and Methods: 45 patients diagnosed with COVID-19 and 45 healthy persons (control group) were included in the study. Vitamin D, ferritin, folic acid, CRP, Ca, Mg and Phosphorus were measured in an autoanalyzer, and SOD, GSH-Px and MDA were spectrophotometrically measured in the serum of the participants. TNF-α, IL-1β and IL6 levels were studied by the ELISA method. Results: The activity of SOD, GSH-px, antioxidant enzymes, Serum vitamin D, folic acid, Ca and Mg of the COVID-19 group was found to be significantly lower than the control group (p<0.05). Conclusion: Again, the levels of MDA, TNF-α, IL-1β, IL-6, CRP and ferritin in the Covid-19 group were found to be significantly higher than in the control group (p<0.05).Antioxidant enzyme activities were low and oxidative stress was high in patients with COVID-19. At the same time, the levels of serum ferritin, CRP, TNF-α, IL-1β and IL6 were high, and levels of Ca and Mg were low in patients with COVID-19.According to these results, we hypothesize think that the level of oxidative stress, inflammation, vitamin D, and serum ferritin, Ca, and Mg levels play a role in the pathogenesis of COVID-19. Future clinical trials should be conducted to further clarify the pathogenesis in patients with COVID-19.