Scopus İndeksli Yayınlar Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3596
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Browsing Scopus İndeksli Yayınlar Koleksiyonu by browse.metadata.publisher "ABADER (Adıyaman Bilimsel Arastırmalar Dernegi)"
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Article Comparison Of Different Life Stages Of Total, Phospholipid And Triacylglycerol Fatty Acids Of Lucilia Sericata(ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2021) Kizmaz, V.Lucilia sericata, which belongs to the Calliphoridae family (Diptera), is used as a debridement tool in open necrotic wounds that do not respond to conventional treatments. Knowing the total, phospholipid (PL), and triacylglycerol (TAG) fatty acid content of L. sericata fly, which is important for health, in its different stages is important both in terms of taxonomy and physiology. After L. sericata samples used in the study were obtained commercially, they were bred under laboratory conditions and then the fatty acids in different stages were analyzed by gas chromatography. Sixteen fatty acids are determined as a result of the analysis. When individual fatty acids are considered, Palmitic acid (16:0), Palmitoleic Acid (16:1n-7), oleic acid (18:1n-9) and Linoleic Acid (18:2n-6) were found to be major ones, while the others are detected in trace amounts. It is determined that, out of total, PL and TAG, saturated fatty acids (SFA) are found at a high percentage in the eggs, monounsaturated fatty acids (MUFA) are the highest in the larvae, and polyunsaturated fatty acids (PUFA) are the highest in fly and pupa. Furthermore, out of total, PL and TAG, ∑PUFA is at a low percentage in the egg and the larval stages. Different results are found at different stages in this study. This may be because fatty acid percentages that change during metamorphosis meet different physiological needs at different phases. © 2021, ABADER (Adıyaman Bilimsel Arastırmalar Dernegi). All rights reserved.Article Effect of Lambda-cyhalothrin on the Gill Phospholipid (PL) Subclass of Oreochromis niloticus(ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2023) Yolcu, Murat; Satar, Elif İpek; Başhan, Mehmet; Kızmaz, VeysiFatty acids have a crucial role in providing energy and performing essential functions in living organisms. Moreover, these substances exhibit the most significant alterations in their structure based on ecotoxicological parameters when viewed from a biochemical perspective. These bioactive chemicals are present in the cellular architecture. The study of these fatty acids, crucial for maintaining the integrity and permeability of cell membranes, holds great significance for all living organisms. Consequently, doing fatty acid analysis specifically at the phospholipid level holds significant importance. The impact of lambda cyhalothrin on the fatty acid content of several phospholipid subclasses (phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) in the gill tissue of O. niloticus (Perciformes: Cichlidae) was assessed using gas chromatography. The alterations in the fatty acid composition was analyzed 21 days after exposure. Following the complete extraction of lipids from gill tissue, the tissue was subsequently separated into different subclasses of phospholipids using thin layer chromatography. The samples were subjected to methylation and then evaluated using Gas Chromatography to determine the percentage of the fatty acid. After doing the analysis, a grand total of 16 fatty acids were identified. The research revealed that the primary fatty acids were 16:0 and 18:0 of saturated fatty acids, monounsaturated 18:1n-9, and polyunsaturated 18:2n-6, 20:4n-6, and 22:6n-6. Upon analyzing the distribution of fatty acids, it was observed that PC, PE, and PI included 16:0, PE contained 18:1, PE and PS contained C18:2n-6 and 20:4n-6, and significant alterations in C22:6n-3 were detected in PE. Our investigation revealed that the n-3/n-6 ratio of fish in the PE subclass was the lowest when compared to PC, PI, and PS.
