Scopus İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3596

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Bioactive Profile, Anticarcinogenic, Antimicrobial, Antidiabetic Effects, and in Silico Pharmacokinetic Properties of Astragalus Elatus
(Academic Press inc Elsevier Science, 2025) Ercan, Leyla; Akan, Hasan; Caliskan, Cemile Gunbegi
Because of the numerous useful components they contain, plants are significant resources that are being explored for their varied purposes in industry, food, and medicine. Recently, particularly endemic species have gained attention with the identification of their different ingredients and characteristics and the exploration of their precious features. This research assessed the endemic species Astragalus elatus's bioactive components utilizing UHPLC-Orbitrap (R)-HRMS, its antibacterial qualities utilizing the disc diffusion method, its cytotoxic impact on cervical cancer utilizing the MTT method, and its apoptosis effect by annexin V methods. Additionally, Astragalus elatus's antidiabetic activity was revealed by in silico and in vitro examinations of its inhibitory effect on the enzymes alpha-Amylase and alpha-Glucosidase. Consequently, it was discovered that A. elatus can be utilized in the treatment of cervical cancer and has effective cytotoxic and apoptotic effects on HeLa cells. Additionally, it was detected that A. elatus possesses antibacterial and anti-diabetic properties. Compared to gram-negative bacteria, A. elatus had a greater inhibitory effect on gram-positive bacteria. The pharmacokinetically significant characteristics and their tendency for chemical reactions of this plant's primary phenolic compounds were identified by utilizing DFT, molecular docking, and ADME/T.
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Citation - Scopus: 1
Myricetin Promotes Migration and Prevents Palmitate-Induced Apoptosis in Cultured Tenocytes Through Ampk-Dependent Pathways
(Academic Press inc Elsevier Science, 2025) Lim, Do Su; Ahn, Sung Ho; Cho, Wonjun; Gwon, Hyeon Ji; Ko, Jun Hwi; Pyo, Min Kyung; Jung, Tae Woo
Myricetin (Myr), a flavonoid present in vegetables and fruits, has been shown to ameliorate inflammation and oxidative stress in various disease models. However, the effects of Myr on hyperlipidemic tenocytes have not been studied. Herein, we aimed to investigate the effects of Myr on the features of tendinopathy in cultured tenocytes under hyperlipidemic conditions. Reactive oxygen species (ROS) were detected by DCFDA. Hydrogen peroxide (H2O2), malondialdehyde (MDA), and caspase 3 activity were quantified via matched assay kits. Apoptotic cells were detected via TUNEL staining. Proteins investigated in this study were evaluated through Western blotting. Treatment with Myr enhanced tenocyte migration and prevented apoptosis, inflammation and oxidative stress in palmitate-treated tenocytes. Myr treatment increased the phosphorylation of AMPK, and the expression of PGC1 alpha and FGF2. siRNA targeting AMPK abrogated the effects of Myr on palmitate-treated tenocytes. However, FGF2 siRNA reduced the impacts of Myr on only cell migration and ECM signaling. These in vitro results suggest that Myr promotes tenocyte migration and ECM signaling via AMPK/FGF2 signaling and attenuates apoptosis through the AMPK-mediated suppression of inflammation and oxidative stress in hyperlipidemic tenocytes. This study sheds light on therapeutic strategies for treating obesity-related tendinopathy.
Ultrastructural Characteristics Of Leishmania (l.)tropica (wright, 1903) and Cell-Parasite Relationships in Cutaneous Leishmaniasis. Light and Electron Microscopic Study
(Academic Press inc Elsevier Science, 2025) Karimbayova, Gulnara N.; Gasimov, Eldar K.; Mahmudov, Farid R.; Rzayev, Fuad H.; Khalilov, Rovshan; Eftekhari, Aziz; Baran, Ayse
A light and electron microscopic study of skin biopsies taken from 9 patients with ulcerative leishmaniasis of both sexes aged from 14 to 26 years in the territory of the Republic of Azerbaijan was carried out. Based on clinical, morphological and electron microscopic parameters, all patients were diagnosed with ulcerative cutaneous anthroponotic leishmaniasis (Leishmania (L.) tropica). Stained and unstained ultrathin (50-70 nm) sections were viewed and photographed using a JEM-1400 transmission electron microscope at an accelerating voltage of 80-120 kV. Analysis of data from light and electron microscopic studies at the ultrastructural level made it possible to describe the structure and identify the morphometric parameters of the amastigote form of the intracellular parasite. Besides, it was found that the distance between the plasmalemmas of the parasitophorous vacuoles and the parasite L. (L.) tropica is only 1 nm. This facilitates the passage of the necessary nutrients for the survival of this parasite. One of the important factors in the chronic course and relapse of leishmaniasis caused by L.(L.) tropica is the penetration of the amastigote stage into the cytoplasm along with macrophages, and also into fibroblasts with low phagocytic activity. Pathological changes (deformed nucleus, damage to plasmalemma, focal destruction of the cytoplasm structures, vacuolization, etc.) in the parasite L. (L.) tropica, localized in macrophages, were identified and described.

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