Ebelik Bölümü Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/225

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Citation - WoS: 10
Citation - Scopus: 11
Carbamazepine-induced renal toxicity may be associated with oxidative stress and apoptosis in male rat
(Taylor & Francis Online, 2021) Erdem Güzel, Elif; Kaya Tektemur, Nalan; Tektemur, Ahmet; Etem Önalan, Ebru
Carbamazepine (CBZ) is the antiepileptic drug used in epilepsy and some psychiatric disorders. Besides its widely used, many adverse effects have been reported including hematotoxicity, hepatotoxicity, endocrine disorders, and testicular damages due to oxidative stress. However, the role of CBZ on renal toxicity is not fully known. In this study, we attempted to explain the connected mechanisms by focusing on the metabolism of CBZ-induced renal toxicity in rats. Twenty male Wistar-Albino rats were randomized into 2 groups (n = 10); control (1 mL/day distilled water, orally) and CBZ (25 mg/kg/day CBZ, orally) groups. After 60 days, TAS (total oxidant status) and TOS (total oxidant status) levels, histopathological features, some genes involved in apoptosis, 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, and apoptotic cells were assessed of kidney tissue. The oxidative stress index (OSI) was measured from TAS and TOS levels. TOS levels and OSI significantly increased, while TAS levels decreased in the CBZ group relative to the control group. Histopathological observations, Caspase-3 (Casp3), Poly [ADP-ribose] polymerase-1 (PARP-1), 8-OHdG immunoreactivities, and apoptotic cells markedly raised in the CBZ group compared with the control group. Also, mRNA expression of Cytochrome c (Cytc) and CASP3 significantly increased in the CBZ group compared to the control group. In conclusion, long-term use of CBZ may promote renal damage in rats by inducing oxidative stress and apoptosis.
Citation - WoS: 3
Citation - Scopus: 6
Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring
(Taylor & Francis Online, 2020) Erdem Güzel, Elif; Nalan Kaya, Ahmet Tektemur, Nazife Ulker, Ahmet Yardimci, Ramazan Fazil Akkoc, Sinan Canpolat & Ibrahim Enver
Prenatal tobacco-smoke exposure negatively affects the reproductive functions of female offspring and oxidative stress plays a major role at this point. Alpha-lipoic acid (ALA), well known as a biological antioxidant, has been used as a nutritional supplement and as a therapeutic agent in the treatment of certain complications during pregnancy. We aimed to investigate the effects of maternal tobacco-smoke exposure and/or ALA administration on puberty onset, sexual behavior, gonadotrophin levels, apoptosis-related genes, apoptotic cell numbers and oxidative stress markers in the adult female rat offspring. Sprague-Dawley rats were divided into four groups; control, tobacco smoke (TS), TS+ALA and ALA groups. Animals were exposed to TS and/or ALA for 8 weeks before pregnancy and throughout pregnancy. All treatments ended with birth and later newborn female rats were selected for each experimental group. The experiment ended at postnatal day 74-77. Maternal tobacco smoke advanced the onset of puberty in the female offspring of the TS group (p < 0.05). In all treatment groups; the mean number of anogenital investigations and lordosis quality scores showed a decline, serum luteinizing hormone levels significantly increased (p < 0.05) and several histopathological changes in ovaries were observed compared to the control group. In addition, an increase in apoptotic marker levels and apoptotic cell numbers was detected in the ovaries of all treatment groups. Decreased TAS and increased TOS levels were detected in all treatment groups compared to control. These findings suggested that maternal tobacco smoke and/or ALA administration may be leading to the impaired reproductive health of female offspring. Abbreviations: ALA: alpha-lipoic acid; LH: luteinizing hormone; FSH: follicle-stimulating hormone; TAS: total antioxidant status; TOS: total oxidant status; Apaf1: apoptotic protease-activating factor 1; Casp3: caspase 3; Casp9: caspase 9; CF: cyst follicles; 4-HNE: 4-Hidroxynonenal; 8-OHdG: 8-hydroxydeoxyguanosine; TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine-biotin nick end labeling; ROS: reactive oxygen species; GnRHR: gonadotropin-releasing hormone receptor; HPG: hypothalamic-pituitary-gonadal; AMPK: AMP-activated protein kinase; ELISA: enzyme-linked immunosorbent assay; cDNA: complementary DNA; qPCR: quantitative real-time PCR; FC: follicular cysts; PF: primary follicle; SF: secondary follicle; GF: graafian follicle; CL: corpus luteum; DF: degenerated follicle; AF: atretic follicle.
Citation - WoS: 3
Citation - Scopus: 3
Chronic Maternal Tobacco Smoke Exposure and/or Alpha-Lipoic Acid Treatment Causes Long-Term Deterioration of Testis and Sexual Behavior in Adult Male Rats
(Elsevier, 2020) Yardimc, Ahmet; Ramazan Fazil Akkoc, Ahmet Tektemur, Nazife Ulker, Nalan Kaya Tektemur, Elif Erdem Güzel, Sinan Canpolat, Ibrahim Enver Ozan
Background: Tobacco use during pregnancy is known to have several negative effects on the offspring's reproductive health in the long term. The use of alpha-lipoic acid (ALA) as a dietary supplement during pregnancy has increased greatly in recent years and has been known to have positive effects on various pregnancy outcomes including miscarriage, diabetic embryopathy, preterm delivery, and congenital malformations. Aim: To evaluate the effects of tobacco smoke exposure (TSE) on sexual behavior, reproductive parameters, and testicles in adult male rats and to reveal the possible role of ALA administration on these parameters. Methods: Pregnant rats (n = 7 per group) were treated with tobacco smoke (TS), ALA (20 mg/kg), and TS + ALA for a total of 11 weeks. The following parameters were compared with 8 control rats: puberty parameters, sexual behavior; levels of serum gonadotropins and testosterone, total antioxidant status, and total oxidant status; the expression of the apoptotic protease-activating factor-1 and caspase 9 mRNA levels in the testis; and assessment of immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling assay of testis. Main Outcome Measure: Sexual behavior, changes in puberty parameters, and hormonal and genetic alterations were the outcomes analyzed in this study. Results: Maternal TSE caused a significant decrease in the number of intromissions compared to the control group. Similarly, ALA decreased erectile function in sexual behavior by decreasing the number of intromissions and intromission ratio in the ALA group compared to the control group. In addition, TSE and ALA treatment caused an impairment of some consummatory sexual behaviors. Also, in parallel with this inhibitory effect, the age of pubertal onset was significantly delayed in the TS + ALA group compared to other groups. Also, histopathological changes in testicular tissue, oxidative stress markers, apoptotic index, and mRNA levels of apoptosis-related genes increased in all treatment groups. Clinical Implications: The use of ALA and/or tobacco products during pregnancy may adversely affect the reproductive health of male newborns in the long term. Strengths & Limitations: To the best of our knowledge, this study is the first to show the effects of maternal ALA treatment and/or TSE on the sexual behavior and reproductive parameters in male rats; however, the study is based on an animal model, and the present findings partially reflect the characteristics of human sexual behavior. Conclusion: Maternal TSE and/or ALA treatment may impair sexual behavior in adulthood in male rats because of testicular damage caused by oxidative stress during gonadal development. Yardimci A, Akkoc RF, Tektemur A, et al. Chronic Maternal Tobacco Smoke Exposure and/or Alpha-Lipoic Acid Treatment Causes Long-Term Deterioration of Testis and Sexual Behavior in Adult Male Rats. J Sex Med 2020;17:1835–1847.
Citation - Scopus: 7
The effect of hospital-based breastfeeding group education given early perinatal period on breastfeeding self-efficacy and breastfeeding status
(ScienceDirect, 2022) Yeşil, Yeşim; Ekşioğlu, Aysun; Ceber Turfan, Esin
Aim: This study was conducted to examine the effect of hospital-based group breastfeeding education provided to mothers before discharge from the hospital on mothers’ self-efficacy and on the increase of breastfeeding rates. Methods: This randomized controlled educational research was conducted in the hospital is located in the West of Turkey. Ninety mothers who had healthy and term babies, and an intent to breastfeed their babies participated in the study. The questionnaire forms were collected by the researcher using face to face interview technique. Chi-square, dependent and independent groups t-test and ANOVA analysis were used along with descriptive statistics in analyzing the data. Results: Compared to the control group, the scores obtained from the Breastfeeding Self-Efficacy Scale were higher in the education group in all the follow-ups. Similarly, status of exclusive breastfeeding was higher in the education group compared to the control group. In the twelfth week, the rate of exclusive breastfeeding was 80.0% in the education group, while it was 47.5% in the control group. Conclusion: These results show that breastfeeding group education and follow-up counseling given before discharge from the hospital were effective. This is evidence in favor of increasing the use of group education. This RCT was registered NCT04515862 with www.clinicaltrials.gov on 2020.
Effects of Maternal Tobacco Smoke or Alpha Lipoic Acid on Puberty Onset, Estrous Cycle and Gonadotropin Levels in Female Rats
(KARGER, 2018) Ülker, Nazife; Yardimcı, Ahmet; Kaya, Nihat; Tektemur, Ahmet; Akkoç, Ramazan Fazıl; Erdem Güzel, Elif; Canpolat, Sinan; Ozan, Enver
Various environmental factors are known to affect puberty onset. However, there are few studies in literature about how maternal tobacco smoke (ts) or alpha lipoic acid (ala) affect the hypothalamus-pituitary-gonadal axis at peripheral or central levels in rats. This study aimed to investigate effects of maternal tobacco smoke or alpha lipoic acid on puberty onset, estrous cycle and serum gonadotropin levels in female rats. Adult female spraque-dawley rats were used. All animals were randomly divided into 4 groups (control, ts, ts+ala and ala) and each group consisted of 7 rats. All ts rats were exposed to ts (20 gram\day, for one hour twice a day) and all ala rats received daily oral ala (20 mg/kg) during 8 week. Afterwards all rats were impregnated, ts or ala treatments continued during pregnancy. All treatments ended with birth and later newborn female rats were selected for each group (n=7). Puberty onset was monitored by examination of vaginal opening in female rat pups. Subsequently, estrous cycle was conducted daily for 15 days and determined by examination of the vaginal smear cytology. Also, serum fsh and lh levels were measured using elisa method at the end of the experiment. There was significantly advanced on puberty onset day for ts group (p<0.05). There was a significantly increase in pubertal weight in ala group compared to control group (p<0.001). The mean total number of estrous cycles and average duration of metestrus, diestrus, proestrus or estrus phases were not significantly different in all treatments groups compared to control group. There was no any significant change in serum fsh levels, but serum lh levels were significantly increased in all treatment groups compared to control group (p<0.05). Present study showed that maternal tobacco smoke or alpha lipoic acid may affect hypothalamus-pituitary-gonadal axis differently in rats.
Citation - WoS: 1
Citation - Scopus: 1
Evaluation of contraceptive intentions among pregnant women in the third trimester: A cross-sectional study
(Wolters Kluwer, 2024) Yeşil, Yeşim; Dönmez, Ayşegül
This study aims to determine contraceptive intentions of pregnant women in the third trimester. This cross-sectional study was conducted at a training and research hospital in Mardin province between March 2023 and May 2023. In total, 253 pregnant women who had the ability to communicate in Turkish and agreed to participate were included in the study. Data were collected through face-to-face interviews using a survey and the Contraceptive Intent Questionnaire (CIQ). The average age of the participants was 28.87 ± 5.35 with 30.8% of them holding a secondary school degree. It was found that there was a statistically significant difference in terms of CIQ score between categories of the following descriptive variables: education level, husband's/partner's education level, fertility plan, receiving family planning counseling during pregnancy, and paying for the contraceptive methods used (P = .046, P = .013, P < .001, P = .008, and P < .001 respectively). It was determined that pregnant women who had not received family planning counseling during pregnancy and paid for the contraceptive method had higher contraception intention.
Citation - WoS: 7
Citation - Scopus: 7
The investigation of effect of alpha lipoic acid against damage on neonatal rat lung to maternal tobacco smoke exposure
(ELSEVIER SCIENCE BV, 2018) Erdem Güzel, Elif; Kaya, Nalan; Ozan, Gonca; Tektemur, Ahmet; Dabak, Durrin Özlem; Ozan, İbrahim Enver
This study was carried out to determine the changes in the lungs of the rat pups exposed to tobacco smoke during pregnancy period and to investigate the protective effects of alpha lipoic acid, which is administered during pregnancy, on these changes. Spraque-Dawley female rats were divided into four groups: control, tobacco smoke (TS), tobacco smoke + alpha lipoic acid (TS + ALA) and alpha lipoic acid (ALA). The rats in control group were untreated. Rats were exposed to TS twice a day for one hour starting from eight weeks before mating and during pregnancy. 20 mg / kg of ALA was administered to rats. On 7th and 21st days 7 of the pups from each group were decapitated. Histological, morphometric, biochemical and quantitative real-time RT-PCR analyzes were performed. Histopathological and biochemical changes were observed in TS group. While a significant decrease was observed both in SP-A and VEGF immunoreactivities and mRNA levels, caspase-3 immunoreactivity and TUNEL positive cells were increased in TS group. It is suggested that prenatal TS exposure leads to morphological and histopathological changes on lung development by causing oxidative damage in lungs of neonatal rats and the maternal use of ALA can provide a limited protective effect on the neonatal lung development against this oxidative stress originating from TS. Although pregnant women are increasingly aware on health risks of smoking, environmental tobacco smoke exposure is still a widespread problem. For this reason, it is thought that this damage can be partially reduced by some antioxidant supplements in pregnancy.
Citation - WoS: 1
Citation - Scopus: 2
Parents' Needs and Their Perceived Obstacles With Team Members in Perinatal Palliative Care: A Qualitative Phenomenological Study
(Journal of Hospice and Palliative Nursing, 2022) Yaşar, Beril Nisa; Terzioğlu, Fusun
Advances in prenatal diagnosis have made medical, psychological, and ethical issues regarding the diagnosis of fetal anomalies a crucial topic of research. This study identified the needs of parents of unborn babies with fetal anomalies and their views regarding the services of interdisciplinary team members in charge of assisting them. The sample consisted of 30 parents and 40 team members from a women's health education and research hospital. Data were collected through face-to-face interviews and were qualitatively analyzed using the phenomenological method of interpretation. The results showed that parents' physical, psychological, social, and spiritual needs during pregnancy, childbirth, and postpartum were not fully met after their unborn babies were diagnosed with fetal anomalies. The results also showed that team members needed a structured program, legal regulations, and training to provide holistic care. © Lippincott Williams & Wilkins.
Citation - WoS: 12
Citation - Scopus: 13
The therapeutic effect of hesperetin on doxorubicin-induced testicular toxicity: Potential roles of the mechanistic target of rapamycin kinase (mTOR) and dynamin-related protein 1 (DRP1)
(Elsevier, 2022) Tektemur, Ahmet; Kaya Tektemur, Nalan; Erdem Güzel, Elif
Clinical utilization of doxorubicin (DOX), which is a commonly used chemotherapeutic, is restricted due to toxic effects on various tissues. Using hesperetin (HST), an antioxidant used in Chinese traditional medicine protects testis against DOX-induced toxicity although the molecular mechanisms are not well-known. The study was aimed to examine the possible role of the mechanistic target of rapamycin kinase (mTOR) and dynamin 1-like dynamin-related protein 1 (DRP1) in the therapeutic effects of HST on the DOX-induced testicular toxicity. Rats were divided into Control, DOX, DOX + HST, and HST groups (n = 7). Single-dose DOX (15 mg/kg) was administered intraperitoneally and HST (50 mg/kg) was administered by oral gavage every other day for 28 days. Total antioxidant status (TAS), histopathological evaluations, immunohistochemistry, and gene expression level detection analyses were performed. Histopathologically, DOX-induced testicular damage was ameliorated by HST treatment. DOX reduced testicular TAS levels and increased oxidative stress markers, 8-Hydroxy-deoxyguanosine (8-OHdG), and 4-Hydroxynonenal (4-HNE). Also, upregulated mTOR and DRP1 expressions with DOX exposure were decreased after HST treatment in the testis (p < 0.05). On the other hand, DOX-administration downregulated miR-150-5p and miR-181b-2-3p miRNAs, targeting mTOR and mRNA levels of beclin 1 (BECN1) and autophagy-related 5 (ATG5), autophagic markers. Furthermore, these levels were nearly similar to control testis samples in the DOX + HST group (p < 0.05). The study demonstrated that HST may have a therapeutic effect on DOX-induced testicular toxicity by removing reactive oxygen species (ROS) and by modulating the mTOR and DRP1 expressions, which have a critical role in regulating the balance of generation/elimination of ROS.

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