Şeker, Uğur

Şeker, Uğur

Job Title

Dr. Öğr. Üyesi

Main Affiliation

Department of Basic Medical Sciences / Temel Tıp Bilimleri Bölümü

Status

Current Staff

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Scholarly Output

10

Articles

10

Citation Count

0

Supervised Theses

0 Scholarly Output Search Results

Now showing 1 - 9 of 9

An Examination of the Effects of Propolis and Quercetin in a Rat Model of Streptozotocin-Induced Diabetic Peripheral Neuropathy
(Mdpi, 2024) Şeker, Uğur; Celik, Hakim; Dagli, Seyda Nur; Taskin, Seyhan; Seker, Ugur; Deniz, Mustafa
The purpose of this study was to reveal the combined effects of propolis (P) and quercetin (Q) against diabetic peripheral neuropathy developing with streptozotocin-induced diabetes in rats. Sixty-four adult male rats were divided into eight equal groups: control, P (100 mg/kg/day), Q (100 mg/kg/day), P + Q (100 mg/day for both), diabetes mellitus (DM) (single-dose 60 mg/kg streptozotocin), DM + P, DM + Q, and DM + P + Q. The rats were sacrificed, and blood and sciatic nerve tissues were collected. Blood glucose and malondialdehyde (MDA) levels increased, while IL-6 and total antioxidant status decreased in the DM group (p = 0.016 and p = 0.047, respectively). Ultrastructural findings showed degeneration of the axon and myelin sheath. The apoptotic index (AI %), TNF-alpha, and IL-1 beta immunopositivity increased significantly in the DM group (p < 0.001). Morphological structures approaching those of the controls were observed in the DM + P, DM + Q, and DM + P + Q groups. Morphometric measurements increased markedly in all treatment groups (p < 0.001), while blood glucose and MDA levels, AI (%), TNF-alpha, and IL-1 beta immunopositivity decreased. In conclusion, the combined effects of propolis and quercetin in diabetic neuropathy may provide optimal morphological protection with neuroprotective effects by reducing hyperglycemia, and these may represent a key alternative supplement in regenerative medicine.
Deneysel Diyabetik Ratlarda İnsizyonel Yara İyileşmesinde Aloe Vera’nın Etkinliğinin Mmp-1 Ve Timp-1 Yönünden İncelenmesi
(2023) Şeker, Uğur; Başaran, Süreyya Özdemir; Soker, Sevda; Kaplan, Özge; Aşır, Fırat; Deveci, Engin; Şeker, Uğur
Purpose: The aim of this study is to investigate the healing aspect of aloe vera in diabetes mellitus, which inhibits wound healing. Materials and methods: Diabetes model was created with streptozotocin. At the end of the 14-day experiment, blood glucose was measured from the tail vein of animals in all groups and blood was taken from the heart and sacrificed. Histopathology and immunohistochemical statistics and evaluation were performed. Results: Pycnosis and degeneration of epithelial cells were observed in diabetes groups. Leukocyte infiltration in the dermal papilla, degeneration of collagen fibers and an increase in the extracellular matrix were observed. It was observed that the epithelial layer in the aloe vera group was histologically close to the control group. It was observed that decreased inflammation in the dermal papilla and decreased in organized collagen fibers and vessel dilatation were observed. In the control group, MMP-1 and TIMP-1 expression were positive in the epidermis and dermis layers. In the diabetes group, weak expression of MMP-1 and TIMP-1 was observed in cells in the epidermis and dermis. The expression of MMP-1 and TIMP-1 in the surface epithelium in the aloe vera group was increased compared to the diabetes group. Conclusion: Aloe vera accelerated cell and extracellular matrix regeneration with its anti-oxidative activity.
Oxidative stress, apoptosis, inflammation, and proliferation modulator function of visnagin provide gonadoprotective activity in testicular ischemia-reperfusion injury
(Verduci Editore srl, 2023) Sağır, Süleyman; Şeker, Uğur; Pekince Özener, Merve; Yüksel, Meral; Demir, Mehmet
OBJECTIVE: Visnagin (Vis) is a compound found in the flowers and seeds of the Ammi visnaga plant with promising antioxidant and anti-inflammatory properties. We aimed to investigate the dose-dependent gonadoprotective effects of visnagin in rats while considering oxidative stress, apoptosis, and inflammation-related protein expression levels. MATERIALS AND METHODS: Twenty-eight adult rats were divided into four groups of seven animals each; control, ischemia/reperfusion (I/R), I/R+30Vis, and I/R+60Vis. Animals in control received no surgical application and were sacrificed at the end of the experiment. The rats in I/R, I/R + Vis30, and I/R + Vis60 were exposed to testicular ischemia and the animals in I/R + Vis30, and I/R + Vis60 groups received either 30 or 60 mg/kg visnagin intraperitoneal. At the end of the experiment, testis tissues were used for the measurement of oxidative stress, apoptosis, and inflammation. RESULTS: Our microscopic examinations indicated that I/R resulted in testicular degenerations and morphological alterations, which were improved in visnagin-treated animals. The biochemical analyses demonstrated that oxidative stress in the I/R group increased significantly (p<0.05) compared to the control group. The immunohistochemical examinations showed that pro-apoptotic Bax and Caspase 3 expressions, and pro-inflammatory tumor necrosis factor-alpha (TNF-α) levels were significantly up-regulated (p<0.05) but proliferating nuclear antigen (PCNA) levels in I/R group was significantly (p<0.001) down-regulated compared to the control group. CONCLUSIONS: Ischemia leading to testicular torsion is a reproductive health-affecting problem, and current surgical treatment methods might be insufficient to recover the testis due to the accumulation of reactive oxygen species (ROS). Our observations indicate that visnagin is a potential co-modality along with the surgical interventions for the recovery of ischemia encountered testis, but we believe the requirement of more detailed studies to explore the underlying signaling pathways and the strength of visnagin against testicular ischemia-reperfusion injury.
Adm and Sflt-1 Expression in Placentas With Gestational Diabetes Mellitus
(2023) Şeker, Uğur; Aşır, Fırat; Deveci, Engin; Arslan, Necat; Kaplan, Özge; Şeker, Uğur; Başaran, Süreyya Özdemir
Amaç: Bu çalışmada gestasyonel diyabetes mellitusta (GDM) vasküler regülasyonda rolü saptanan iki yeni protein olan Adrenomedullin (ADM) ve soluble fms-benzeri tirozin kinaz (sFlt-1)’in ekspresyon seviyelerini incelemeyi, hastalığın histopatolojisinde bu proteinlerin ekspresyon seviyelerini karşılaştırmayı ve bu proteinlerin ekspresyon yoğunluğunun hastalıkla korelasyonunu gözlemlemeyi amaçladık. Gereç ve Yöntem: Çalışmamızda 20 Normotansif ve 20 GDM’li plasenta örneği alındı. Histolojik takip yöntemiyle takip edildi. Bu dokulardan 5µm kalınlığında kesitler alınarak Hematoksilen-Eozin, Periodic Acid Schiff (PAS) boyamaları yapıldı. İmmunünohistokimyasal olarak ADM ve sFlt-1 antikorları çalışıldı. Bulgular: GDM grubunda; kök villuslarındaki kan damarlarında dilatasyon ve konjesyon, endotel hücrelerinde hiperplazi görüldü. Villusların dış kısmındaki sinsitiyal köprülerde artış, mononükleer hücre infiltrasyonu, maternal bölgedeki desidual hücrelerin bazılarında piknotik nükleuslar ve sitoplazma kaybı izlendi. İmmunohistokimyasal incelemede villusların sitotrofoblast ve sinsitiyotrofoblast hücrelerinde ve sinsitiyal düğümlerde negatif ADM ekspresyonu vardı. Küçük villusların bazı sitotrofoblast hücrelerinde, damar endotel hücrelerinde ve desidual hücrelerde pozitif ADM ekspresyonu görüldü. GDM grubunda sFlt-1 ekspresyonu endotel hücrelerinde, mezenşimal bağ doku içindeki bazı Hofbauer hücrelerinde, desidual hücre nükleuslarında ve membranlarında pozitif olarak gözlendi. Sonuç: Desidual hücre membranlarında, sitotrofoblastlarda ADM pozitif ekspresyon gösterdiğinden ADM’nin glikoz yoğunluğunun belirlenmesinde ve bununla ilişkili olarak insülin regülasyonunda önemli bir düzenleyici olabileceğini düşündürmüştür. Yine sFlt-1’in maternal ve fötal bölgelerdeki endotel hücresi üzerindeki etkileri ve Hofbauer hücrelerindeki ekspresyonu, anjiyogenik etkide bu molekülün anahtar rol alabileceği kanısını uyandırmıştır.
Investigation of the Protective Activity of Baicalein on the Lungs via Regulation of Various Cellular Responses in Rats Exposed to Experimental Sepsis
(Oxford University Press, 2023) Dicle, Yalçın; Şeker, Uğur; Şeker, Uğur
Backgrounds In the present study, a cecal ligation and puncture (CLP)-induced experimental sepsis rat model was used to explore the effects of baicalein on inflammatory cytokine levels and oxidative stress as well as the possible regulatory role of nuclear factor-kappa B (NF-κB). Methods For that purpose, 42 Wistar albino rats were equally divided into control, sham, sepsis, B50 + S, B100 + S, S + B50, and S + B100 groups. The B50 + S and B100 + S groups received baicalein before the induction of sepsis, while the S + B50 and S + B100 groups received baicalein afterwards. Experimental sepsis in related groups is generated through ligation of cecum and a puncture in cecal wall. Serum samples were used for tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) analyses, and tissue Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione (GSH), IL-6, and NF-κB levels were measured. Results Compared to the control group, there were significantly increases in the serum TNF-α, IL-6, tissue MDA, and NF-κB levels and decreases in the tissue SOD and GSH levels in the septic group (P < 0.05). Compared to the septic group, inflammation and oxidative stress were reduced in the baicalein-treated groups. Although all of the pre- and post-treatment protocols alleviated inflammation and oxidative stress to varying degrees, pre-treatment with 100 mg/kg was the most successful. Conclusions Findings of this study indicated that baicalein has the potential to reduce sepsis-related oxidative stress and inflammation in the lungs and that pathological outcomes could be regulated via NF-κB transcription factor activity.
Ameliorating effects of low-dose ketamine administrations on opioid-induced memory impairments and neurodegeneration in mice
(İnönü Üniversitesi Tıp Fakültesi, 2023) Şeker, Uğur
Aim: Opioids have indispensable roles in pain management. A strong link exists between opioid use and memory impairments, mainly with continuous use. This study investigated the effects of two opioid drugs, meperidine and fentanyl, on emotional memory functions, brain morphology, and the possible protective effects of low-dose ketamine in mice. Materials and Methods: A passive avoidance (PA) test was used to measure emotional memory functions following seven daily drug applications in 48 male Balb/C mice (30-35 g). Meperidine (10 mg/kg), fentanyl (0.3 mg/kg), ketamine (5 mg/kg), and combinations of ketamine with the opioids were intraperitoneally injected daily. No drugs were utilized during the testing days. Brain tissues were obtained after sacrification and put into diluted formalin solution for histopathological analysis. Results: Transfer latencies of the meperidine and fentanyl-treated groups in the PA test were lower than in the vehicle-treated group (p<0.01, p<0.05, respectively). Ketamine combined with meperidine had higher latencies than in the meperidine-treated group (p<0.05). The augmenting effects of ketamine were evident against fentanyl and meperidine-induced neurotoxicity as morphologic alterations were reduced. Conclusion: Low-dose ketamine may fend against opioid-induced neurotoxicity and emotional memory impairments, especially against meperidine, which can be a practical alternative to fentanyl in clinical settings.
Normozoospermik Fertil Bireyler ile Oligozoospermik, Şiddetli-Oligozoospermik, Oligoastenozoospermik, Azoospermik ve İdiyopatik Bireylerde Natural Killer Hücre Aktivitesinin Araştırılması
(Mardin Artuklu Üniversitesi, 2023) Şeker, Uğur; Afşin, Muhammet; Şeker, Uğur; Yavuz, Dilek; Bademkıran, Muhammed Hanifi; Yıldırım, İbrahim Halil; Cirit, Ümüt
Giriş: İnfertilite çiftleri ve toplumu birçok açıdan olumsuz etkileyen ve gittikçe yaygınlaşan bir sağlık problemidir. Bu problemlerin %30-40’ının erkek kaynaklı olduğu tahmin edilmektedir. Amaç: Farklı derecelerde infertilite problemi olan erkek bireyler ile normal sperm sayısı ve konsantrasyonuna sahip bireylerde (normozoospermi) doğal öldürücü (Natural Killer: NK) hücre aktivitesinin değişip değişmediğinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Sperma analizleri sonucu oligozoospermi, şiddetli-oligozoospermi, oligoastenoozospermi, azoospermi ve idiopatik olduğu belirlenen bireyler ile normozoospermi teşhisi konan bireylerden (n:120) alınan kan numunelerinden NK hücre aktiviteleri ölçüldü. Bulgular: Yapılan ölçümler sonucunda normozoospermi grubu en düşük değer (544.46 pg/ml) alırken şiddetli oligozoospermi grubundan en yüksek değer (1005.90 pg/ml) alınmıştır. NK hücre aktivitesi ise oligozoospermi, oligoastenozoospermi, azoospermi ve idiyopatik gruplarda sırasıyla 797.60 ± 428.55 pg/ml, 905.34 ± 430.60 pg/ml, 757.66 ± 541.16 pg/ml ve 639.44 ± 385.50 pg/ml olarak ölçüldü. Şiddetli oligozoospermi grubu ile diğer gruplar arasında NK aktivitesi farkı önemli (p<0.05) bulunurken diğer gruplar arasındaki farklar önemli bulunmamıştır (p >0.05). Sonuç: İnfertilite derecesi şiddetli oligozoospermi olan bireylerde NK hücre aktivitesinin normozoospermi, oligozoospermi, oligoastenozoospermi, azoospermi ve idiopatik gruplarından daha yüksek olduğu belirlenmiştir.
Effects of acute carbon monoxide posioning on liver damage and comparisons of related oxygen therapies in a rat model
(Taylor & Francis Ltd, 2024) Gökdemir, Gül Şahika; Gokdemir, Gul Sahika; Seker, Ugur; Şeker, Uğur; Demirtas, Berjan; Taskin, Seyhan
Acute carbon monoxide (CO) poisoning may cause liver damage and liver dysfunction. Therefore, in this study, we aimed to compare the efficiency of normobaric oxygen (NBO) and high-flow nasal cannula oxygen (HFNCO) treatments on liver injury. For that purpose, 28 male Wistar albino rats were divided into four groups (Control, CO, CO + NBO, and CO + HFNCO). The control group was allowed to breath room air for 30 min. Acute CO poisoning in CO, CO + NBO, CO + HFNCO was induced by CO exposure for 30 min. Thereafter, NBO group received 100% NBO with reservoir mask for 30 min. HFNCO group received high-flow oxygen through nasal cannula for 30 min. At the end of the experiment, all animals were sacrificed by cardiac puncture under anesthesia. Serum liver function tests were measured. Liver tissue total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels, tissue histomorphology and immunoexpression levels of Bax, Caspase 3, TNF-alpha, IL-1 beta, and NF-kappa B were also examined. Our observations indicated that acute CO poisoning caused significant increases in blood COHb, serum aminotransferase (AST), alanine aminotransferase (ALT0, alkaline phosphatase (ALP), total protein, albumin, and globulin levels but a decrease in albumin to globulin ratio (all, p < 0.05). Furthermore, acute CO poisoning significantly increased the OSI value, and the immunoexpresssion of Bax, Caspase 3, TNF-alpha, IL-1 beta, and NF-kappa B in liver tissue (all, p < 0.05). These pathological changes in serum and liver tissue were alleviated through both of the treatment methods. In conclusion, both the NBO and HFNCO treatments were beneficial to alleviate the acute CO poisoning associated with liver injury and dysfunction. [GRAPHICS] .
The nephroprotective effect of Quercetin in Cyclophosphamide-induced renal toxicity might be associated with MAPK/ERK and NF-κB signal modulation activity
(Taylor & Francis Ltd, 2024) Seker, Ugur; Şeker, Uğur; Kavak, Deniz Evrim; Dokumaci, Fatma Zehra; Kizildag, Sefa; Irtegun-Kandemir, Sevgi
The present study aimed to examine the protective effect of quercetin (QUE) on cyclophosphamide (CTX)-induced nephrotoxicity. For that purpose, 24 mice were divided into four groups (Control, QUE, CTX, and CTX + QUE). The CTX and CTX + QUE groups received 200 mg/kg of cyclophosphamide on the 1(st) and 7(th) days. The QUE and CTX + QUE groups were treated with 50 mg/kg of quercetin daily for 14 days. At the end of the experiment, the animals were sacrificed, and kidney samples were analyzed. The results indicated that CTX leads to severe morphological degenerations and disruption in renal function. Serum BUN, Creatinine, Uric acid, tissue Bax, Caspase 3, TNF-alpha and IL-1 beta expression levels were upregulated in the CTX group compared to Control and QUE groups (p < 0.05). Although MAPK/ERK phosphorylation level is not affected in CTX group, there was a significant increase in CTX + QUE group (p < 0.05), but the NF-kappa B was significantly suppressed in this group (p < 0.01). The RT-qPCR results showed that the cyt-c and the Bax/Bcl-2 ratio mRNA expression folds were upregulated in the CTX group (p < 0.01), which was downregulated in the CTX + QUE group. However, there was a significant difference in the CTX + QUE group compared to the Control and QUE groups (p < 0.01). The findings showed that administering quercetin along with cyclophosphamide alleviated renal injury by regulating apoptotic and inflammatory expression. Moreover, the administration of quercetin and cyclophosphamide could synergistically improve renal function test results, and activate cellular responses, which upmodulate MAPK/ERK phosphorylation and suppression of NF-kappa B.