Examining the Angiogenic and Antioxidant Effects of Various Paracetamol Dosages Using a Chorioallantoic Membrane Model

Abstract

Introduction: Paracetamol is one of the most popular and frequently used analgesic and antipyretic medications in the world. It was aimed at investigating the effects of paracetamol on angiogenesis and oxidative stress markers in the in vivo chorioallant oic membrane model (CAM). Materials and Methods: The 40 fertilized chick eggs were used in the experiment. The four groups were as; the control group (n = 10), 10-6 M bevacizumab group (n = 10), 10-4 M paracetamol group (n = 10) and 10-5 M paracetamol group (n = 10). Stereoscopic microscopy was used to assess angiogenesis on the window that was opened on the eggshell. Total oxidant capacity (TOS), total antioxidan t capacity (TAS), and oxidative stress index (OSI) were analyzed in albumen specimens. The scoring methodology described in earlier research publications was used to determine and compare the average score values. One-way analysis of variance and the post hoc Tukey test were performed to assess oxidative stress markers between the groups. Results: The bevacizumab group demonstrated a strong anti-angiogenic effect, but the control group and both paracetamol groups showed no anti-angiogenic effect. Paracetamol increased TAS values at a 10-4 M concentration. The bevacizumab group's TOS and OSI values were significantly higher compared to the control group. Conclusion: Paracetamol is used by a lot of people; healthy blood vessel growth and the balance between oxidants and antioxidants are important. The important result of this study is that paracetamol has strong antioxidant effects and no anti-angiogenic effects. © 2025, Van Medical Journal. All rights reserved.