Sağlık Hizmetleri Meslek Yüksekokulu

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Anoxybacillus sp. SO B1-Immobilized Amberlite XAD-16 for Solid-Phase Preconcentration of Cu(II) and Pb(II) and Their Determinations by Flame Atomic Absorption Spectrometry
(TAYLOR & FRANCIS INC, 2015) Oral, Elif Varhan; Ozdemir, Sadin; Dolak, Ibrahim; Okumus, Veysi; Dundar, Abdurrahman; Ziyadanogullari, Berrin; Aksoy, Zehra; Onat, Ruken
A new method for the determination of Cu(II) and Pb(II) by flame atomic absorption spectrometry (FAAS) after preconcentrating on a column containing Anoxybacillus sp. SO B1-immobilized Amberlite XAD-16 was developed. The functional groups of Anoxybacillus sp. SO B1 immobilized on Amberlite XAD-16 were characterized in KBr tablets by Fourier transform infrared (FT-IR) spectrometry. Various parameters such as pH, amount of the adsorbent, eluent type and volume, and flow rate of the sample solution were studied. The optimum pH values of quantitative sorption for Cu(II) and Pb(II) were found to be pH 7.0 and 5.0 and Cu(II) and Pb(II) ions could be quantitatively eluted with 5.0ml of 1.0mol L-1 HCI and 10.0ml of 0.25mol L-1 HNO3, respectively. Recoveries of Cu(II) and Pb(II) were found to be 100.9 +/- 1.57% and 100.3 +/- 0.49% (N = 5), the limits of detection of Cu(II) and Pb(II) in the determination by FAAS (3s, N = 10) were found to be 0.8 and 1.6 mu g L-1, respectively. The proposed enrichment method was applied for metal ion determination from water samples such as two parts of Tigris River water in Diyarbakir and Elazig, Lake of Hazar in Elazig, and tap water in Diyarbakir. Furthermore, the accuracy of the proposed method was verified by studying the analytical recovery and by analyzing certified reference material (NCS-DC 73350 leaves of poplar).
ANTİ-KANSER İLAÇ YÜKLENMİŞ MANYETİK O-KARBOKSİ METİL KİTOSAN NANOKOMPOZİTİN SİTOTOKSİSİTE ÇALIŞMALRI
(2019) Tarhan, Tuba; Tural, Bilsen; Tural, Servet
Günümüzde kanser kemoterapisinin en önemli sorunlarına çözüm bulmak için nanoboyutta ilaç taşıyıcı sistemler geliştirilmiştir. Nanoboyuttaki kontrollü ilaç taşıma sistemleri tümör hedeflenmesine olanak sağlamıştır. Nanotaşıyıcılar arasından, biyouyumlu polimer kaplı süperparamanyetik nanoparçacıklar dışardan uygulanan manyetik alan ile istenilen bölgeye hedeflenebilme özelliklerine sahiptirler. Bu sayede ilaç hedeflenen bölgeye güvenli bir şekilde taşınabilmektedir. Bu çalışmada daha önce sentez ve karakterizasyonu yapılmış Manyetik O-Karboksimetil Kitosan nanokompozitine anti kanser ilaç olan Topotekan yükleme çalışmaları yapılmış olup, burada topotekan yüklü manyetik O-Karboksimetil Kitosan nanokompozitinin prostat kanseri Du145 ve sağlıklı insan prostat PNT1A hücre hattı üzerine sitotoksisite çalışmaları yapılmış ve sonuçları değerlendirilmiştir. Anahtar Kelimeler: Polimer Kaplı Süperparamanyetik Nanokompozit, Topotekan, Sitotoksisite, DU145 ve PNT1A
Application of Half-Sandwich Metal-Phosphinite Compounds to Biological Activities: Determine the energies of the HOMO and LUMO levels
(Wiley Online Library, 2022) Meriç, Nermin; Rafikova, Khadichakhan; Zazybin, Alexey; Güzel, Remziye; Kayan, Cezmi; Karakaş, Duygu Elma; Dündar, Abdurrahman; ISLAM, Sholpan; Okumuş, Veysi; Aydemir, Murat
Mononuclear transition metal complexes 1-(furan-2-yl)ethyldiphenyl[dichloro(η6-p-cymene)ruthenium(II)]phosphinite, (2), 1-(furan-2-yl)ethyldiphenyl[dichloro(η6-benzene) ruthenium(II)] phosphinite (3), 1-(furan-2-yl)ethyldipheny[chloro(η4-1,5-cyclooctadiene)rhodium(I)]phosphinite (4), 1-(furan-2-yl)ethyldiphenyl[dichloro (η5pentamethylcyclopentadienyl)iridium (III)] phosphinite (5) were synthesized and characterized by microanalysis, infrared, MS, and NMR spectroscopy. The biological activities of the complexes were also tested. Compounds 2 and 5 were the best complexes at DPPH radical scavenging and reducing power activity at 73.27 % and 0.41 at 200 μg/mL, respectively. The highest antimicrobial activity exhibited by complex 3 as 14 mm inhibition zone against S. aureus. All of the complexes have cleaved the DNA from the double-strand and exhibited three bands on gel electrophoresis. Moreover, cyclic voltammetry studies of the phosphinite complexes were carried out to determine the energies of the HOMO and LUMO levels as well as to estimate their electrochemical and some electronic properties.
Association with Leptin Gene c.-2548 G > A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients
(HUMANA PRESS INC, 2013) Say Şahin, Deniz; Tümer, Cemil; Demir, Cemil; Çelik, M. Murat; Çelik, Mustafa; Uçar, Edip; Güneşaçar, Ramazan
Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G > A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G > A polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G > A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p < 0.001) and body mass index (p < 0.0001). All these consequences showed that LEP -2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population.
Biological assays, electrochemical behavior, and theoretical DFT calculations of Ru(II) complexes of chiral phosphinite based based on β-amino alcohols: Transfer hyrogenation of ketones using a HCOOH/Et3N mixture
(Elsevier, 2024) Rafikova, Khadichakhan; Meriç, Nermin; Ertekin Binbay, Nil; Okumuş, Veysi; Sever, M. Şerif; Belyankova, Yelizaveta; Tursynbek, Saniya; Dauletbakov, Anuar; Sarah, Bayazit; Zolotareva, Darya; Yerassyl, Kamshyger; Dündar, Abdurahman
Synthesis of two phosphinite ligands based on β-amino alcohols, in high yields has been demonstrated. When we treated [Ru(arene)(μ-Cl)Cl]2 {arene:p-cymene,benzene} with chelating phosphinite ligands, we obtained neutral Ru(II)-complexes possessing the general formula [Ru(arene)phosphiniteCl2]. The structure of the ligands and complexes was confirmed using analytical and spectroscopic techniques. The quantum chemical calculations were carried out for the ruthenium complexes at the DFT/CAM-B3LYP level of theory in gas phase. The phosphinite complexes were subjected to cyclic voltammetry studies in order to determine the energies of HOMO and LUMO levels and to estimate their electrochemical and some electronic properties. Organic complex-based memory substrates were immobilized using TiO2-modified ITO electrodes, and the memory functions of phosphinite-based organic complexes were verified by chronoamperometry (CA) and open-circuit potential amperometry (OCPA). In the present study, the antioxidant potentials of ruthenium-based p-cymene and benzene complexes through DPPH radical scavenging, metal chelating, and reducing power activities were also determined. In addition, DNA binding abilities and antimicrobial activities of these complexes against pathogenic bacteria were studied. Finally, the ruthenium complex, (2S)-1-{[(2S)-2-[(diphenylphosphanyl)oxy]propyl][(1R)-1-phenylethyl]amino}propan-2-yldiphenyl phosphinitobis[dichloro(η6-benzene)ruthenium(II)] also catalyzed asymmetric transfer hydrogenation of acetophenone with high conversion (up to 99%) and good enantioselectivity (ee up to 89 %), in the existence of formic acid and triethylamine in dichloromethane medium under air atmosphere.
Biosorption of chlorophenoxy acid herbicides from aqueous solution by using low-cost agricultural wastes
(TAYLOR & FRANCIS INC, 2015) Okumus, Veysi; Celik, K. Serdar; Ozdemir, Sadin; Dundar, Abdurrahman; Kilinc, Ersin
The potential biosorption abilities of apple shell (AS), orange peel (OP), banana peel (BP), and millet waste (MW) as a bio-waste material to remove 2,4-dichlorophenoxy acetic acid (2,4-D), 2,4-dichlorophenoxy propanoic acid (2,4-DP), and 2,4-dichlorophenoxy butyric acid (2,4-DB) from aqueous solution were investigated in batch condition. Optimum biosorption conditions were determined as a function of contact time, pH of the solution, amount of biomass, and initial pesticide concentrations. The concentrations of the pesticides in the remaining solutions were simultaneously determined by high performance thin layer chromatography. Langmuir and Freundlich models were applied to describe the biosorption isotherm of the pesticides by agricultural wastes. The experimental adsorption data were fitted to Langmuir adsorption models (r(2)>0.99). It was found that adsorption of 2,4-DP was higher for all biosorbent. The highest maximum adsorption capacities of 2,4-DP were found as 40.08, 22.71, 33.26, and 45.45mg/g, respectively, for AS, OP, BP, and MW. Maximum adsorption capacity was obtained for 2,4-D as OP>MW>BP>AS, 2,4-DP as MW>AS>BP>OP, and 2,4-DB as OP>AS>BP>MW. From the results, it can be said that MW was an effective biosorbent for removal of 2,4-DP and OP was an effective biosorbent for removal of 2,4-D and 2,4-DB through the studied pesticides. Optimum biosorption conditions were determined as 60min of contact time, 100mg of biomass, AS at pH 6.0, OP at pH 6.0, MW at pH 7.0, and BP at pH 7.0. FT-IR was employed to understand the surface properties of biosorbents. According to the results, agricultural wastes have high adsorption capacity.
Boletus edulis loaded with gamma-Fe2O3 nanoparticles as a magnetic sorbent for preconcentration of Co(II) and Sn(II) prior to their determination by ICP-OES
(SPRINGER WIEN, 2018) Özdemir, Sadin; Yalçın, M. Serkan; Kılınç, Ersin; Soylak, Mustafa
The authors show that the fungus Boletus edulis loaded with gamma-Fe2O3 nanoparticles is a viable sorbent for magnetic solid phase extraction of trace levels of Co(II) and Sn(II). The surface structure of immobilized magnetized B. edulis was characterized by FT-IR, SEM and EDX. Experimental parameters were optimized. Following elution with 1 M HCl, the ions were quantified by ICP-OES. The limits of detection are 21 pg.mL(-1) for Co(II), and 19 pg.mL(-1) for Sn(II). The preconcentration factors are 100 for both ions. The sorption capacities of the sorbent are 35.8 mg.g(-1) for Co(II) and 29.6 mg.g(-1) for Sn(II). The method was applied to the analysis of certificated reference materials and gave >= 95% recoveries with low RSDs. It was also successfully applied to the quantification of Co(II) and Sn(II) in spiked environmental and food samples.
BOR NİTRÜR VE TÜREVLERİNİN BİYOMEDİKAL ALANDA UYGULAMALARI
(2022) Tarhan, Tuba
Bor nitrür (BN), eşit sayıda bor ve nitrojen atomundan oluşan kovalent bir katıdır. Sergilediği olağanüstü özellikler sayesinde oldukça dikkat çekmiş ve çığır açacak yeni uygulamalar için beklentileri artırmıştır. Yapısal olarak karbon sistemine benzeyen BN'ler dört farklı poli kiristal formda bulunur: grafit benzeri altıgen BN (h-BN), eşkenar dörtgen (r-BN), elmas benzeri kübik BN (c-BN) ve wurtzite BN (w-BN) (1-3). Özellikle h-BN, iki boyutlu (2D) katmanlı yapı sunan standart koşullar altında en kararlı BN fazıdır. Bor atomları için sp2 hibridizasyonu gözlemlenir ve B-N-B veya N-B-N arasında beklenen bağ açısı 120˚ olup, grafit gibi mükemmel bir altıgen halka bağ ağı oluştururken, 2D katmanlar zayıf van der Waals kuvvetleri tarafından bir arada tutulur (2,4,5). BN nanomalzemeleri, nanoküreler, nanotüpler (BNNT), nanolifler, nanofulleren (BNFL), kuantumdot (BNQD) nanoşeritler (BNNR), ince filmler ve nanolevhalar (BNNS) gibi tüm morfolojik şekillerde bulunabilir (Şekil 1) (6,7).İlgi çekici kimyasal, fiziksel ve biyolojik özelliklerinden dolayı bor nitrür, bilimsel ve teknolojik topluluklar tarafından büyük ilgi görmüştür (4). BN malzemeleriyle ilgili yayınların sayısı son on yılda çarpıcı bir biçimde arttmıştır (bkz. Şekil 2a). Şekil 2b, 2019'daki BN materyallerinin yayın durumunu göstermektedir: yaklaşık %50'si nanomalzemelerle ilgilidir ve hem BNNT'ler hem de BNNS'ler üzerine yapılan araştırmalar yaklaşık %20'sini oluşturmaktadır. Bu raporlara göre BN sistemleri optoelektronik cihazlar (1,8,9), kuantum yayıcılar (1,10,11), yarı iletkenler (1,12), enerji depolama (1,13), termal iletkenler (1,14), kozmetik ürünler (1,15), su temizleme (1,16,17), fotokatalitik (1,18) ve ayrıca biyomedikal bilimi (1,19) vb. üzerine yapılan çalışmalar ile ilgili verileri içermektedir (1).Bu bölümde, odak noktasının in vivo ve in vitro olduğu, bor nitrürün kedisi, türevleri, bir kaplama malzemesi veya kompozit halinin biyouyumluluğu ve anti-bakteriyel özelliklerinden dolayı biyomedikal uygulamalarda kullanılabilme potansiyelleri araştırılıyor. Bor nitrür, ilaç dağıtımı, görüntüleme ve hücre stimülasyonu gibi tıbbi alanlarda bazı uygulamalar için yeni bir kapı açacaktır (20). Ayrıca, BN nanoparçacıkları van der Waals, hidrofilik, hidrofobik, hidrojen bağı ve π-π etkileşimleri gibi moleküller arası etkileşimlerin yanı sıra kovalent olmayan etkileşimler ile işlevselleştirilebilmesi sayesinde, konakçı sistemlere güçlü bir şekilde bağlanır (4,21). Moleküllerin sahip olduğu bu fiziksel etkileşimlerin (konjuge, yüzey aktif maddeler vb.) avantajlarını kullanarak kontrollü fizikokimyasal özellikleri ile farklı ortamlara homojen bir şekilde dağılırlar (4, 22). Literatürde, selüloz filmler veya lifler (4,23,24), kitosan (4,25), polietilenimin aşılanmış nanoşerit (mikro RNA'nın tanınması için) (4,26), vinil asetat kopolimeri (4,22), oktadesilamin (4,27), glukoz oksidaz biyoalgılama (4,28), poli (etilen glikol) (4,29), DNA (4,30) ve metalik nanopartiküller gibi farklı nanoparçacıkları içeren kovalent ve kovalent olmayan çeşitli işlevselleştirme metodolojileri sunulmuştur (4). Bu bileşiklerin başarısı ve performansı için en önemli konu, her türlü inorganik veya biyolojik sistem için en uygun işlevselleştirme sürecinin (biyo-nanomühendislik) seçimidir (4). Fiziksel-kimyasal modifikasyonlar ve uygulamaları için en önemli adım, nanokompleksin kimyasal olarak kararlı, iyi dağılmış ve ilgili biyolojik ortamla uyumlu olmasıdır. Böylece tanı ve tedavi amaçlı ekolojik paketleme, biyo-robotlar, tümör belirteçleri gibi biyoteknoloji ve biyomedikal alanlarda gelişmiş uygulamalara sahip akıllı nano sistemler üretilebilmektedir (4). Bu bölümde, bor nitrürün işlevselleştirilmesinin ana yöntemleri, bunların biyoteknoloji ve nanotıp alanındaki gelişmeleri ve potansiyel uygulamaları hakkında kısa bir rapor sunuyoruz.
Competitive adsorption of VOCs (benzene, xylene and ethylbenzene) with Fe3O4@SiO2-NH@BENZOPHENONE magnetic nanoadsorbents
(Elsevier, 2023) Güngör, Çetin; Ece, Mehmet Şakir
Volatile organic compounds (VOCs), which are toxic, mutagenic and carcinogenic, are considered a critical factor for air pollution and cause serious harm to the eco-environment and human health. In this study, Fe3O4, Fe3O4@SiO2-NH2, Fe3O4@SiO2-NH@BENZOFENONE were synthesized as new magnetic nanoadsorbents (MNAs) and used for the first time in the removal of gas-phase benzene, xylene and ethylbenzene. The synthesised MNAs were characterized by SEM-EDS, TEM, FTIR, XRD, VSM, TGA and BET analyses. The characterization results showed that the MNAs have mesoporous structure, type IV physioresorption and type H3 hysteresis loop character. In order to clarify the comparative and competitive adsorption behaviour, the adsorption capacity of Fe3O4@SiO2-NH@BENZOFENONE MNA was found to be in the order of xylene > ethylbenzene > benzene in both single, binary and ternary component systems. The adsorption kinetics of benzene, xylene and ethylbenzene with Fe3O4@SiO2-NH@BENZOFENONE MNA were found to be governed by multistep mechanisms. Fe3O4@SiO2-NH@BENZOFENONE MNA showed reuse efficiencies of 83.07%, 84.35% and 82.99% after 5 cycles for benzene, xylene and ethylbenzene respectively. In the framework of the results, Fe3O4@SiO2-NH@BENZOPHENONE MNA, which has a high potential in terms of both adsorption capacity and reuse efficiency, is proposed as a promising adsorbent for the efficient removal of benzene, xylene and ethylbenzene. © 2023 Elsevier B.V.
Competitive Biosorption of Azo-Dyes in Aqueous Solution on Magnetic Biosorbent Nanoparticles
(2018) Tarhan, Tuba; Tural, Bilsen; Tural, Servet
Abstract— In this study, glutaraldehyde cross-linked magnetic chitosan nanoparticles (GMCNs) were prepared through crosslinking modification of magnetic chitosan nanoparticles (MCNs) using glutaraldehyde as crosslinker that exhibited excellent adsorption performance Reactive Black 5 (RB5) and Metanil Yellow (MY) in dyes mixture solution. The characterization of synthesized GMCNs was performed by fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) analyses. Adsorption characteristics of dyes mixture solution on to GMCNs have been studied and results indicated that the adsorption capacities were affected by initial dye concentrations and contact time. The adsorption of dyes mixture solution experiments were well fitted the pseudo-second-order reaction. The technique used in this study offers a convenient and economical method for the preparation of nanoparticles, which can facilitate a higher adsorption capacity and thus more efficient adsorption of dyes in an aqueous solution compared with nature or other synthetic materials Keywords— Azo Dye, Biosorption, Biosorption isotherm, Biosorption kinetics, Magnetic Biosorbent
Crystal structure, IR and NMR spectra of (E)-2‑methoxy-4-(2-morpholinovinyl)phenol molecule and its DFT calculations
(ScienceDirect, 2023) Arici, K.; Altınoz, D.E.; Cavusoglu, M.; Diken, S.
In this study; molecular structure, IR and NMR spectroscopic properties of (E)-2–methoxy-4-(2- morpholinovinyl)phenol are investigated both experimentally and theoretically for the first time. The structural characterization of the compound was carried out by X-ray diffraction technique. The bond lengths, bond angles and dihedral angles of the compound were optimized using DFT with the B3LYP 6–311G(dp) base set. Using the optimized results, the infrared vibration frequencies and intensities of the compound as well as the 1H and 13C chemical shift values in the NMR spectrum were calculated with the B3LYP 6–311G(dp) basis set. In addition, the potential energy distributions (PED) of the structure were calculated to determine some characteristic vibrational frequencies of the compound. It is found that the theoretically calculated data support the experimental results. As a result, there is a good agreement between the experimentally determined structural parameters, vibrational frequencies, chemical shifts and theoretically calculated data of the compound.
Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron
(Elsevier, 2020) Cengiz, Mustafa; Şahintürk, Varol; Cetik Yildiz, Songul; Kurcanay Şahin, İlknur; Bilici, Namık; Onur Yaman, Suzan; Altuner, Yılmaz; Appak-Baskoy, Sıla; Ayhanci, Adnan
Background Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.
Determination of Essential Oil Composition, Antimicrobial and Antioxidant Properties of Anise (Pimpinella anisum L.) and Cumin (Cuminum cyminum L.) Seeds
(ANKARA UNIV, FAC AGR, 2014) Hasimi, Nesrin; Tolan, Veysel; Kizil, Suleyman; Kilinc, Ersin
In this research, the essential oil components, antimicrobial and antioxidant properties of anise (Pimpinella anisum L.) and cumin (Cuminum cyminum L.) seed oils were investigated. The essential oil components determined by GC/MS instrument. The antimicrobial activity determined by disc diffusion method against the gram negative bacteria, namely Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, the gram positive bacteria namely Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC19615 and Candida albicans ATCC10231 as the yeast. The antioxidant activities of essential oils were carried out by DPPH free radical scavenging activity method. It was determined that the main components of the anise essential oil were trans-anethole (52.94%) followed by iso-anethole (13.89%), caryophllene oxide (8.55%) and caryophyllene (29.4%); the main components of the cumin essential oil were beta-pinene (15.77%), alpha-terpinene (15:52%), 1-Phenyl-1-butanol (15:13%), cumic aldehyde (12.74%) respectively. Anise essential oil showed weak antimicrobial activity and cumin essential oil showed moderate antimicrobial activity against test microorganisms. Cumin essential oil showed strong antimicrobial activity against C. albicans with 22 +/- 0.9 mm inhibition zone diameter. P. aeruginosa showed resistance to both essential oils. The antioxidant activity of cumin essential oil (75.60%) was observed closer to ascorbic acid (78.75%), higher than BHA (50.45%) and BHT (23.54%). Although anise essential oil exhibited low activity, it has the similar activity with BHT. The scavenging activity of the samples decreasing order was ascorbic acid> cumin >BHA>BHT> anise.
Determination of hydroxymethylfurfural in Turkish honeys, pekmez (grape molasses), and jam samples by high-performance liquid chromatography with diode array detection
(Wiley Online Library, 2022) Elmastas, Ayhan; Aydin, Firat; Umaz, Adil; Kılınc, Ersin; Arslan, Yasin; aydin, Isil
Hydroxymethylfurfural (HMF) is a chemical contaminant that is not naturally present in carbohydrate-containing food samples. The HMF amounts were changed between 4.8 and 214.1 mg/kg in honey; between 7.8 and 204.0 mg/kg in pekmez (grape molasses); between 5.9 and 22.4 mg/kg in strawberry jam; between 6.0 and 65.4 mg/kg in apricot jam; and between 9.2 and 55.9 mg/kg in cherry found by HPLC. The linear calibration plot of HMF was ranged from 0.05 to 10.0 mu g/ml. The correlation coefficient was found to be 0.99997. LOD and LOQ values were found to be 12.0 and 40.0 ng/ml, respectively. According to the experimental results, HMF amounts of four honey samples, three pekmez (grape molasses) samples, one apricot jam, and one cherry jam for investigated samples were found to be higher than the permission amount of Turkish Food Codex. Novelty impact statement Based on experimental results in this study, if the honey is in a transparent package such as a glass, it should be stored in a dark environment. It would be healthier to prefer molasses produced by reliable brands at low temperatures under vacuum instead of molasses made with traditional methods in open boilers. Generally, since jams with high HMF amount have an overcooked or even burnt flavor, this is an extremely negative situation for consumers.
Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C-60 modified gamma-Fe2O3 superparamagnetic iron oxide nanoparticles
(ROYAL SOC CHEMISTRY, 2015) Kılınç, Ersin
In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C-60 modified maghemite (gamma-Fe2O3 superparamagnetic iron oxide nanoparticles - SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL(-1) with a limit of detection of 0.06 ng mL(-1). The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL(-1). The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL(-1).
Determination of zinc in dried nuts by slotted quartz tube-flame atomic absorption spectrometry (SQT-FAAS) with citric acid-coated magnetic nanoparticle (CAMNP) based solid phase extraction (SPE)
(Taylor & Francis inc, 2025) Umaz, Kader; Umaz, Adil; Aydin, Isil; Aydin, Firat
This study reports a sensitive method for trace zinc using slotted quartz tube-flame atomic absorption spectrometry (SQT-FAAS) with citric acid-coated magnetic nanoparticle-solid phase extraction (CAMNP-SPE). To increase the efficiency, we univariately optimized the pH, buffer volume, CAMNP mass, eluent concentration and volume, mixing type and time, temperature, and interaction time. The study identified the limits of detection and quantification values for CAMNP-SPE-SQT-FAAS under the specified optimum conditions to be 12.30 and 41.00 mu g L-1, respectively. The newly developed method improved the detection power of traditional FAAS by 13.25 times. The findings of this study report a sensitive and accurate method to determine trace zinc in complex matrices.
Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II)
(Analytical Biochemistry, 2021) Kılınç, Ersin; Ozdemir, Sadin; Şen, Fatih; Soylak, Mustafa
In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min−1 of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL−1, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
Development of Novel Fe3O4/AC@SiO2@1,4-DAAQ Magnetic Nanoparticles with Outstanding VOC Removal Capacity: Characterization, Optimization, Reusability, Kinetics, and Equilibrium Studies
(Industrial and Engineering Chemistry Research, 2021) Ece, Mehmet Şakir; Kutluay, Sinan; Şahin, Ömer; Horoz, Sabit
The adsorption of pollutants to the surface of adsorbents plays a critical role in the effectiveness of adsorption technology for air purification applications. Herein, novel magnetic nanoparticles functionalized with 1,4-diaminoanthraquinone (1,4-DAAQ), namely, Fe3O4/activated carbon (AC)@SiO2@1,4-DAAQ, were innovatively synthesized via co-precipitation and sol-gel techniques. After that, these nanoparticles were used for high-efficiency removal of volatile organic compounds (VOCs) (i.e., benzene and toluene). The synthesized nanoparticles were characterized by various techniques such as Fourier transform IR spectroscopy, thermogravimetric analysis/differential thermal analysis, scanning electron microscopy, and Brunauer-Emmett-Teller analysis. The dynamic adsorption process of VOCs was optimized based on operating parameters. The adsorption experiments revealed that Fe3O4/AC@SiO2@1,4-DAAQ showed exceptional performance for the removal of VOCs. It was observed that for benzene, Fe3O4, AC, Fe3O4/AC, Fe3O4/AC@SiO2, and Fe3O4/AC@SiO2@1,4-DAAQ exhibited dynamic adsorption capacities of 180.25, 228.87, 295.84, 382.10, and 1232.77 mg/g, respectively. Additionally, for toluene, they exhibited dynamic adsorption capacities of 191.08, 274.53, 310.26, 421.30, and 1352.16 mg/g, respectively. This indicated that the modification of 1,4-DAAQ could greatly enhance the dynamic adsorption capacity of Fe3O4/AC@SiO2@1,4-DAAQ for VOCs. In addition to the apparent adsorptive behavior in removing VOCs, Fe3O4/AC@SiO2@1,4-DAAQ exhibited high repeatability. After ten consecutive adsorption/desorption cycles, for benzene and toluene, Fe3O4/AC@SiO2@1,4-DAAQ retained 79.36 and 78.24% of its initial adsorption capacity, respectively. According to the characterization results, the average pore diameter for Fe3O4/AC@SiO2@1,4-DAAQ was determined to be 24.46 nm, indicating that they were in the mesopore range. The adsorption mechanism of the VOCs on Fe3O4/AC@SiO2@1,4-DAAQ was clarified by investigating the isotherm and kinetic criteria in detail. Isotherm models suggested that the adsorption process of VOCs is physical. Moreover, from the analysis of diffusion-based rate-limiting kinetic models, the findings reveal a combination of intraparticle diffusion as well as film diffusion throughout the adsorption process of VOCs. In addition, it was concluded from the analysis of the mass transfer model factors that global mass transfer and internal diffusion are more effective than film diffusion. The results demonstrated that the Fe3O4/AC@SiO2@1,4-DAAQ nanoadsorbent is a promising material for the effective removal of VOCs.
Disiplinlerarası Yaklaşımın Temel Dayanakları
(Pegem Akademi, 2023) Işıker, Yakup
Eğitim alanındaki yaklaşımlar, öğrencilere daha etkili ve anlamlı öğrenme deneyimleri sunmak amacıyla sürekli bir gelişim ve değişim süreci içerisindedir. Bu sürecin bir ürünü olarak disiplinlerarası yaklaşım, günümüz eğitiminde önemli bir rol oynamaktadır. Disiplinlerarası yaklaşım, Jean-Jacques Rousseau ve John Dewey gibi öğrenme teorisyenlerinin katkılarıyla beslenmiş bir yaklaşımdır (Sözer, 2002). Temel amacı, öğrencilere daha kapsamlı bir öğrenme deneyimi sunarak, bilgi ve becerilerin sınırlarını aşmalarını sağlamak olan bu yaklaşımın dayandığı temel dayanakları anlamak, disiplinlerarası yaklaşımın eğitim alanında nasıl bir değişim ve dönüşüm oluşturduğunu anlamamıza ve bu yaklaşımdan etkili bir şekilde faydalanmamıza yardımcı olacaktır (Gardner, 2006). Disiplinlerarası yaklaşımın bilişsel ve psikolojik dayanaklarını incelediğimizde, öncelikle bilişsel bilimlerde disiplinlerarasılığın yeri ve önemine eğilmemiz gerekmektedir. Bilişsel bilimler, farklı disiplinlerden gelen içgörüleri ve yöntemleri birleştirmek için başarılı bir çaba göstermekle birlikte bu disiplinlerin algı, hafıza, öğrenme, problem çözme vb. kuralların, kavram, imge ve analojilerin zihinde nasıl işleyebileceğine dair birçok üretken teori sunar (Thagard, 2010). Ayrıca disiplinlerarası etkileşimden yararlanarak, davranış ve düşüncenin zihinsel temsillerle nasıl bütünleştiğini ve karmaşık sonuçlara yol açan süreçlerden nasıl etkilendiğini araştırır. Bu yaklaşım, insan zihninin işleyişini çok yönlü ve derinlemesine anlamada önemli bir temel oluşturur ve farklı disiplinlerin bir araya gelerek zengin bir bakış açısı sunmasını sağlar (Kırklaroğlu, 2018). Bilişsel bilimlerdeki bu disiplinlerarası yaklaşımın eğitim alanında da önemli yankıları bulunmaktadır. Eğitimde disiplinlerarası yaklaşımın, öğrencilere çok yönlü düşünme yetenekleri kazandırma, farklı zekâ türleri arasında ilişkiler kurma ve öğrenilenleri günlük yaşamda etkili bir şekilde kullanma amaçlarına yönelik temel hedefleri bulunmaktadır. Bu hedeflerle birlikte öğrencilerin bilgiyi bütünsel bir şekilde birleştirmeleri ve kendi ihtiyaçlarına uygun şekilde oluşturmaları ele alındığında disiplinlerarası yaklaşımın temel dayanaklarının; bilişselcilik, yapılandırmacılık, beyin temelli öğrenme, çoklu zekâ ve Gestalt kuramı gibi yaklaşımlar çerçevesinde temellendiğini söyleyebiliriz (Doğruluk, 2022). Disiplinlerarası yaklaşımın felsefi dayanaklarını ele aldığımızda ise, ilk olarak pragmatizmin bu yaklaşımda öne çıktığını görmekteyiz. Pragmatizmde, öğrencilerin farklı disiplinlerden gelen bilgi ve becerileri birleştirerek gerçek dünya problemlerini çözmeleri gerektiği vurgulanır. Bu noktada günlük hayatta karşılaştığımız sorunlar ve bu sorunlarla ilgili soruların cevaplanması, birden fazla disiplini kapsayan bir çaba gerektirir hale gelmiştir. Örneğin insan hakları, çevreyi koruma, yoksulluk ve şiddet gibi sosyoekonomik ve politik konular, teknolojik ilerlemelere rağmen hala insanlık için büyük bir sorun olmaya devam etmektedir. Bu nedenle, bu sorunların disiplinlerarası bir ortamda bütünsel olarak ele alınması gerekmektedir. Bu disiplinler arasındaki entegrasyon, güncel sorunların çözümüne katkı sağlayacak ve çok yönlü bireylerin gelişimine yardımcı olacak anlamlı bir örüntü oluşturacaktır. Bu sebeple, öğrenme ve öğretme ortamlarında tek disiplinli yaklaşımdan disiplinlerarası yaklaşıma geçmek zorunlu hale gelmiştir (Ulusoy, 2007; Ürey, 2022). Bu doğrultuda, pragmatizm yaklaşımında, eğitim sürekli değişen bir olgu olarak ele alınır ve gerçek dünya problemlerine yönelik disiplinlerarası çözümler üretilmeye çalışılır (Ergün, 2017). Pragmatizm ile birlikte disiplinlerarasılığın felsefi temel dayanaklarından bir diğerinin ilerlemecilik yaklaşımı olduğunu söylemek mümkündür (Doğruluk, 2022). Eğitim alanında oldukça yenilikçi fikirlere sahip olan pragmatizm, eğitimle ilgili fikirlerini “ilerlemecilik” adı altında ortaya koymuştur (Sünney, 2020). İlerlemecilik yaklaşımına göre, eğitimin temel unsuru sürekli olarak yeniden yapılandırılan deneyimlerdir. Geçmişteki deneyimler gelecekteki davranışları etkilediğinden, deneyimler sürekli olarak yeniden yapılandırılır. Pragmatizm'in de etkisiyle ilerlemeciliğe göre eğitim sürekli değişen bir olgu olarak ele alınır ve eğitim, bireyi yaşama hazırlayan bir unsur olmaktan çok yaşamın kendisidir (Ergün, 2017). Bu yönüyle İlerlemeci eğitim anlayışı, toplumun ihtiyaç duyduğu bir anlayış olarak öne çıkar. Sonuç olarak, ilerlemecilik yaklaşımı eğitimde olduğu gibi disiplinlerarası çalışmalarda da temel bir rol oynar. Sürekli deneyimlerin ve bilgilerin yeniden yapılandırılması, farklı alanlardaki ilerlemeyi destekleyen bir dinamizm oluşturur. Bu sayede, toplumsal ihtiyaçlar daha etkili bir şekilde karşılanabilir ve disiplinlerarası ilerleme sağlanabilir.
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.

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