Browsing by Author "Baran, Ayşe"

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Citation - WoS: 2
Citation - Scopus: 3
Activated Carbon-Coated Iron Oxide Magnetic Nanocomposite (ionps@ctac) Loaded With Morin Hydrate for Drug-Delivery Applications
(Frontiers Media Sa, 2024) Dogan, Yusuf; Ozic, Cem; Ertas, Erdal; Baran, Ayse; Rosic, Gvozden; Selakovic, Dragica; Eftekhari, Aziz; Doğan, Yusuf; Baran, Ayşe; Department of Plant Protection / Bitki Koruma Bölümü
Cancer is a major disease that affects millions of people around the world every year. It affects individuals of all ages, races, and backgrounds. Since drugs used to treat cancer cannot distinguish between cancerous and healthy cells, they cause systemic toxicity along with serious side effects. Recently, controlled drug-release systems have been developed to reduce the side effects caused by anticancer drugs used for treatment. Morin is an anticancer drug with a flavonol structure. It has been extensively researched for its antioxidant, anti-inflammatory, antitumoral, and antibacterial properties, especially found in Chinese herbs and fruits, and its multiple positive effects on different diseases. In this study, a nanocomposite with magnetic properties was synthesized by coating biocompatible activated carbon obtained using the fruits of the Celtis tournefortii plant on the surface of iron oxide magnetic nanoparticles. Characterization of the synthesized activated carbon-coated iron oxide magnetic nanocomposite was confirmed by Fourier transform infrared, scanning electron microscopy, energy-dispersive X-ray spectrometry, X-ray diffraction, dynamic light scattering, zeta potential, and vibrating sample magnetometry. The cytotoxic effects of the drug-loaded magnetic nanocomposite were examined in HT-29 (colorectal), T98-G (glioblastoma) cancer cell lines, and human umbilical vein endothelial cell (HUVEC) healthy cell line. The morin loading and release behavior of the activated carbon-coated iron oxide magnetic nanocomposite were studied, and the results showed that up to 60% of the adsorbed morin was released within 4 h. In summary, activated carbon-coated iron oxide magnetic nanocomposite carriers have shown promising results for the delivery of the morin drug.
Adsorption of Crystal Violet Dye With Selenium Nanoparticles Obtained by Green Synthesis From Cherry (prunus Avium L.) Fruit Stalk
(2024) Baran, Ayşe; Turna, Talip; Baran, Ayşe
The rapid development of the global production printing and dyeing industry has led to an increase in the demand for various dyes. Crystal violet (CV), a versatile dye, is widely used in the textile industry and other applications. The reason for its widespread use is its effectiveness and the vivid color it gives to fabrics.CV dye is a water-soluble, toxic, resistant organic dye that is quite dangerous for the ecosystem and causes environmental pollution. Therefore, it must be removed before being released into the recipient environment. This study synthesized selenium nanoparticles (Se NPs) from agricultural Prunus avium L. (PaL.) wastes and removed CV dye. In batch adsorption tests, the effects of pH, amount of adsorbent, time, initial concentration, and temperature were investigated. In this study, where 3 different kinetic and isotherm models were tested, it was determined that the most suitable kinetic and isotherm models for the removal of CV dye with PaL-Se NPs were Pseudo second order (R2:0.999) and Langmuir (R2:0.997), respectively. Additionally, the maximum adsorption capacity (qmax) was calculated as 142.61 mgCV/g PaL-Se NP. Accordingly, it can be said that low-cost PaL-Se NPs synthesized by environmentally friendly methods are a suitable alternative for the removal of CV dye.
Citation - WoS: 13
Citation - Scopus: 12
Adsorption of nickel(II) ions from aqueous solutions using Malatya clay: Equilibrium, kinetic, and thermodynamic studies
(Environmental Progress & Sustainable Energy, 2023) Baran, Ayşe; Dal, Mehmet Can; Onursal, Nilgün; Altunkaynak, Yalçın
This paper evaluates Malatya clay (MC) for the removal of Ni(II) ions from aqueous solutions. For adsorption efficiency, the impacts of several factors, such as pH, starting metal ion concentration, and contact duration, were examined. The optimal initial Ni(II) ion concentration was determined to be 5 mg/L, the adsorption contact duration was 360 min, and the solution's pH was 6.27. Using scanning electron microscopy, energy dispersive spectroscopy, and Fourier transform infrared spectroscopy, the surface characteristics of Malatya clay were examined. The isotherm data fit the Langmuir isotherm model, indicating chemical adsorption and likely irreversibility of the process, according to the results. At 298, 308, and 318 K, the adsorption capacities of Ni(II) were determined to be 10.267, 11.834, and 12.285 mg/g, respectively. Adsorption kinetic data revealed that the adsorption of Ni(II) metal ions followed the pseudosecond-order kinetic model. According to thermodynamic studies, Ni(II) ion adsorption on the MC is spontaneous and endothermic. MC has proven to be an effective and alternative material for the removal of Ni(II) ions from aqueous media due to its excellent removal ability, availability, and low cost, as well as its features such as being able to be used without any chemical treatment and being environmentally friendly.
Amygdalus Communis (badem) Meyvesinden Elde Edilen Özütlerin Bazı İn Vitro Biyolojik Aktivitelerinin, Kimyasal ve Pestisit Kompozisyonlarının Araştırılması
(2024) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Çetik Yıldız, Songül; Tarhan, Rozerin; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Amygdalus communis (A. communis) yüksek düzeyde protein ve doymamış yağ asitlerinden oluşur ve bu da onları doğal kaynakların sınırlı olması gereken diyet beslenmesinde faydalı kılar. Bu çalışmada badem meyvesinden elde edilen kloroform ve metanol özütlerinin antioksidan, antikolinesteraz, antimikrobiyal özellikleri, kimyasal bileşen miktarı ile ağır metal kompozisyonu ve pestisit kalıntı miktarları araştırıldı. A. communis meyvesinde en yüksek toplam fenolik bileşen ve flavonoid içeriğin kloroform özütünde olduğu ayrıca kloroform özütünün DPPH radikal söndürme aktivitesinin daha yüksek olduğu belirlendi. A. communis meyve kloroform ve metanol özütlerinin Escherichia coli ve Staphylococcus aureus bakteri suşları ve Candida albicans mayasının üremesi üzerinde standart antibiyotikler ile kıyaslandığında düşük konsantrasyonlarda oldukça güçlü inhibisyona sebep olduğu belirlendi. A. communis meyve metanol ve kloroform özütlerinin kimyasal bileşen içerikleri LCMS/MS kullanılarak belirlendi. Metanol (Me-OH) özütünde ana bileşenlerin sırasıyla sitrik asit, şikorik asit ve 4-Hidroksibenzoik asit kloroform özütünde ise 4-Hidroksibenzoik asit, p-kumarik asit ve klorogenik asit olduğu belirlendi. A. communis meyve numunesi için pestisit kalıntı analizi GC-MS ve LCMS-MS kullanılarak yapıldı. Test edilen numune içeriğinde Deltametrin kalıntısının varlığı belirlendi. Elde edilen veriler değerlendirildiğinde A. communis meyvesinin zengin biyokimyasal içeriği ve güçlü antibakteriyel, antioksidan ve antikolinesteraz enzim inhibisyon kapasitesi ile biyomedikal ve gıda endüstrilerinde potansiyel uygulamalara sahip olması beklenmelidir.
Citation - WoS: 6
Citation - Scopus: 5
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Citation - WoS: 0
Analysis of Bioactive Compounds Using Lc-esi-ms/Ms, Cytotoxic, Antimicrobial Effects, and Enzyme Activities From Cyclotrichium Origanifolium (Vol 101, Pg 740, 2023)
(Wiley, 2024) Aktepe, Necmettin; Keskin, Cumali; Atalar, M. N.; Baran, F.; Baran, Ayşe; Taskin, A.; Jahan, I; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Anticancer Activities of Zinc Oxide Nanoparticles (znonps) Synthesized From Mentha Longifolia L. Leaf Extract
(2024) Hatipoğlu, Abdulkerim; Baran, Mehmet Fırat; Baran, Ayşe; Baran, Ayşe; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü
Recently, there has been a remarkable increase in cancer and cancer-related deaths. In this study, the impacts of zinc oxide nanoparticles (ZnONPs) produced from the aqueous leaf extract of Mentha longifolia L. (ML) on ovary adenocarcinoma (OVCAR-3), colorectal carcinoma (HCT-116), and healthy retinal pigment epithelial cell (RPE-1) lines were investigated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was performed to discover its antiproliferative properties. As a result of the application of ML-ZnONPs on RPE-1, OVCAR-3, and HCT-116 cell lines at doses (µg/mL) of 250, 500, and 1000 for 24 hours, the viability rates (%) in the cell lines were 18.73-30.56, 21.98-28.76, and 27.27-40.93, respectively. In the 48-hour application, the viability rates (%) of the same cells were between 29.51-46.83, 32.49-40.81, and 46.82-44.37, respectively. The MTT test revealed that ML-ZnONPs strongly suppressed the growth of RPE-1, OVCAR-3, and HCT-116 cell lines. The test showed that the effect of dose increase and time on the viability of both cancer cell lines was negative.
Citation - WoS: 0
Citation - Scopus: 0
Antioxidant Properties of allium Turcicum Özhatay & Cowley Plant Extract, Its Effects on the Proliferation and Migration of Cancer Cells
(Frontiers Media Sa, 2024) Baran, Ayşe; Baran, Mehmet Fırat; Cebe, Deniz Baris; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Firat; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum & Ouml;zhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum & Ouml;zhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 mu g/mL, respectively. We conducted a cell migration experiment to study the A. turcicum & Ouml;zhatay & Cowley Extract (AT & Ouml;CE) impact on cancer cells' metastatic behavior. Our findings indicate that AT & Ouml;CE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of AT & Ouml;CE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 +/- 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 +/- 0.044 mg GAE/g.
Citation - WoS: 1
Citation - Scopus: 2
Antioxidant, Ache Inhibitory, and Anticancer Effects of Verbascum Thapsus Extract
(C M B Assoc, 2023) Keskin, Cumali; Baran, Ayşe; Valioglu, Ferzane; Teng, Lei; Atalar, Mehmet Nuri; Keskin, Cumali; Beilerli, Aferin; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Verbascum thapsus (Mullein) is a medicinal plant used in folk medicine to treat various ailments. For this study, the biological functions of Verbascum thapsus (VT) methanol extract were determined in vitro. The plant's methanol extract was created through the maceration process. The phytochemical composition of plant extracts was investigated using liquid chromatography-electrospray ionization tandem mass spectrometry. The antioxidant capacity of the extract was determined using the 2,2-diphenyl-1-picrylhydrazil (DPPH radical) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS radical). Cell lines Caco-2 (human colorectal adenocarcinoma cells), LNCaP (Lymph Node Carcinoma of Prostate), and HEK293 (Human embryonic kidney 293 cells) were used to model colon, prostate, and non-cancerous cells. The cytotoxic activity of the plant extract on the proliferation of these cells was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay protocol. VT extract showed moderate DPPH and ABTS radical scavenging activities at 30 mg/ml concentration. With this, VT extract was determined to inhibit acetylcholinesterase (AChE) enzyme and had strong cytotoxic activity on cancerous cell lines. In addition, our findings clearly showed that the plant extract had greater cytotoxic activity on the viability of cancerous cells compared to non-cancerous (Human embryonic kidney cells; HEK293) cells. The current findings showed that V. thapsus might be a promising anti-cancer medication candidate for the treatment of human colorectal adenocarcinoma and colon cancer, as well as a good source antioxidans. Copyright: (c) 2023 by the C.M.B. Association. All rights reserved.
Artemisia Absinthium Bitki Ekstraktından Altın Nanopartiküllerin Çevre Dostu Hızlı Sentezi ve Antimikrobiyal Aktivitelerin Uygulanması
(2021) Keskin, Cumali; Baran, Mehmet Fırat; Baran, Ayşe; Baran, Ayşe; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Nanopartiküller, üstün özelliklerinden dolayı birçok alanda kullanılmaktadır. Son zamanlarda, bu parçacıkların çevre dostu sentez yöntemleri kullanılarak üretilmesi çok popüler hale gelmiştir. Bu bağlamda, bu çalışmada, altın nanopartiküller (AuNP'ler), Artemisia absinthium'un (pelin otu) yeşil yaprak özütü ile hızlı ve çevre dostu bir yöntem kullanılarak sentezlenmiştir. AuNP'lerin tane boyutu Debye-Scherrer denklemine göre 13.40 nm olarak belirlendi. AuNP'lerin test edilen mikroorganizmaların büyümesi üzerindeki inhibe edici etkileri, minimum inhibe edici konsantrasyon (MİC) yöntemi ile belirlenmiştir. MİC, bir mikroorganizmanın büyümesini engelleyen bir antimikrobiyal maddenin minimum konsantrasyonu olarak tanımlanmaktadır. Elde edilen AuNP'ler, sırasıyla 0.250, 0.125, 0.125, 0.033 ve 0.66 mg mL-1 konsantrasyonlarında S. aureus, S. pyogenes, E. coli, P. aeruginosa suşları ve C. albicans mayası üzerinde önleyici etkiler göstermiştir.
Citation - WoS: 5
Citation - Scopus: 5
Biochemical components, enzyme inhibitory, antioxidant and antimicrobial activities in endemic plant Scilla mesopotamica speta
(Wiley, 2021) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Akmeşe, Şükrü; Akmeşe, Şükrü; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, in vitro antioxidant, antimicrobial, anticholinesterase and phenolic profile of different solvent extracts of Scilla mesopotamica speta were determined in detail. In vitro antioxidant activities and total phenolic and flavonoid contents of plant extracts obtained with different solvents were tested in terms of 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities. The highest total phenolic and total flavonoid contents were determined in the ethyl acetate extract (62.24 mu g GAE/mg) and chloroform extract (87.72 mu g QE/mg) respectively. The highest DPPH radical scavenging activity was detected in ethyl acetate extracts. Antimicrobial and antifungal activities were investigated by MIC method. The inhibitory activities of the extracts on the acetyl cholinesterase enzyme were investigated. Liquid chromatography (LC) tandem mass spectrometry LC-MS/MS was used to determine the phenolic component content of extracts. Thirty-one different components were identified in the analyses and their amounts were measured. Practical applications Scilla mesopotamica speta is an endemic and medicinal plant. It was determined that the extracts of this plant had a very rich content in terms of phenolic compounds, especially caffeic and ferulic acids. However, this plant was remarkable for its antioxidant, anticholinesterase, and antimicrobial activities. Considering the strong antioxidant, antimicrobial, and enzyme inhibition activities of the Scilla mesopotamica speta it can be suggested as a source of anticancer, antimicrobial, and antiviral drugs.
Citation - WoS: 5
Citation - Scopus: 4
Biosynthesis of AgNPs by extract from waste leaves of Citrullus lanatus sp. (watermelon); characterization, antibacterial and antifungal effects
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe; Department of Nursing / Hemşirelik Bölümü
Silver nanoparticles (AgNPs) are valuable materials with a large number of sectors used. Green synthesis is very important for biomedical applications as they show biocompatible properties. In this study, AgNPs were easily synthesized using the environmentally friendly green synthesis approach using agricultural waste parts of Citrullus lanatus sp. plant grown in Diyarbakır region. Characterization of synthesized AgNPs was made. Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to evaluate the phytochemicals responsible for effective reduction in the formation of AgNPs. UV-visible spectrophotometer (UV-Vis.) Spectra were also used to determine the presence of AgNPs. X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope, Zeta potential analyzes were performed to define the crystal structures, dimensions and surface charges of AgNPs, respectively. In these data, it was determined that AgNPs showed maximum absorbance at 460 nm, spherical appearance, 21.27 crystal nano size and -30.05 mV zeta potential. Antimicrobial effects of AgNPs on gram positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) bacteria, gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) bacteria, as well as on fungus C. albicans pathogenic microorganisms It was analyzed by specifying the Minimum Inhibition Concentration (MIC) by microdilution.
Citation - WoS: 58
Citation - Scopus: 67
Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract
(Elsevier, 2023) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Aytuğ Ava, Canan; İrtegün Kandemir, Sevgi; Cebe, Deniz Barış; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Metallic nanoparticle biosynthesis is thought to offer opportunities for a wide range of biological uses. The green process of turning biological waste into utilizable products gaining attention due to its economical and eco-friendly approach in recent years. This study reported the ability of Solanum tuberosum (ST) peel extract to the green synthesis of non-toxic, stable, small-sized silver nanoparticles without any toxic reducing agent utilizing the phytochemical components present in its structure. UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, flourier scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and energy dispersive analysis X-ray confirmed the biosynthesis and char-acterization of silver nanoparticles. Also, dynamic light scattering and thermogravimetric ana-lyses showed stable synthesized nanoparticles. The antibacterial activity of the biosynthesized silver nanoparticles was evaluated against four different bacterial strains, Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) Bacillus subtilis (B. subtilis), and a yeast, Candida albicans (C. albicans) using the minimum inhibitory concen-tration technique. The cytotoxic activities were determined against Human dermal fibroblast (HDF), glioblastoma (U118), colorectal adenocarcinoma (CaCo-2), and human ovarian (Skov-3) cell lines cancer cells using MTT test. The nanoparticle capping agents that could be involved in the reduction of silver ions to Ag NPs and their stabilization was identified using FTIR. Nano -particles were spherical in shape and had a size ranging from 3.91 to 27.07 nm, showed crys-talline nature, good stability (-31.3 mV), and the presence of capping agents. ST-Ag NPs significantly decreased the growth of bacterial strains after treatment. The in vitro analysis showed that the ST-Ag NPs demonstrated dose-dependent cytotoxicity against cell lines. Based on the data, it is feasible to infer that biogenic Ag NPs were capped with functional groups and demonstrated considerable potential as antibacterial and anticancer agents for biomedical and industrial applications.
Celtis Tournefortii Lam Yaprak Özütünün Bileşenlerinin Lc-ms/ms Aracılığıyla Belirlenmesi, Enzim İnhibisyonunun, Antimikrobiyal ve Antikanser Etkilerinin Araştırılması
(2023) Keskin, Cumali; Baran, Ayşe; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Bitkilerden elde edilen özütlerde bulunan fitokimyasallar çok önemli biyoaktif bileşenlerdir. Bu çalışmada, Celtis tournefortii Lam. ağacı (CT) yapraklarından elde edilen özüt içeriğindeki fitokimyasallar LC-MS yöntemi ile belirlendi. Rutin (2479.89 µg ml-1), kumarin (1241.68 µg ml-1), biyokanin A (1026.42 µg ml-1), şikimik asit (477.32 µg ml-1) ve klorojenik asit (300.76 µg ml-1) bileşenlerinin yüksek konsantrasyonlara sahip olduğu belirlendi. CT ekstraktının patojen suşların büyümesi üzerinde baskılayıcı etkileri mikrodilüsyon yöntemi ile çalışıldı. 2.00-8.00 µg ml-1 konsantrasyon aralığında suşlar üzerinde baskılamaya neden olduğu gözlendi. Özütün asetil kolinesteraz ve glutatyon-S transferaz enzim aktiviteleri üzerindeki inhibisyon etkileri incelendi ve enzim aktivitesinin %50 inhibitör değerleri sırasıyla 13.58 ve 13.86 olarak bulundu. 3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazol bromür tekniği uygulanarak CT yaprak özütünün hücre canlılığını baskılayıcı etkileri yumurtalık, kolon ve beyin kanseri hücrelerinin yanı sıra sağlıklı cilt fibroblast hücrelerinde çalışıldı. Kanser hücrelerinde sırasıyla %42, %4.27 ve %14.29 oranında baskılama oluşturduğu gözlendi.
Celtis Tournefortii Lam. Bitkisinin Yeşil Yapraklarından Gümüş ve Altın Nanopartiküllerin Biyolojik Yolla Sentezi, Karakterizasyonu, Kimyasal Kompozisyon ve İn-vitro Antimikrobiyal ve Sitotoksik Aktivitelerinin Belirlenmesi
(2024) Keskin, Cumali; Baran, Ayşe; Keskin, Cumali; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Celtis tournefortii Lam. (C.tournefortii) bitkisinin atık durumundaki yeşil yapraklarından elde edilen özüt kullanılarak iki farklı konsantrasyonda altın nanopartiküllerin (AuNP'lerin) ve gümüş nanopartiküllerin (AgNP'lerin) sentezi, ucuz, kolay ve çevre dostu bir yaklaşımla yapıldı. Sentez sonucunda elde edilen AuNP'lerin ve AgNP'lerin karakterizasyonu UV-vis Spektrofotometre, XRD, FTIR, TEM, AFM, FESEM, EDX, TGA-DTA, DLS cihazlarından elde edilen veriler aracılığıyla değerlendirildi. 5 ve 10 mM'da sentezlenen AuNP'lerin sırasıyla, küresel morfolojik görünümde, negatif yüzey yük dağılımında, 553.67-555.32 nm maksimum absorbansa sahip ve 31.30-53.55 nm boyut dağılımında oldukları belirlendi. 5 ve 10 mM'da sentezlenen AgNP'lerin sırasıyla, 479.10-482.13 nm maksimum absorbansta, negatif yüzey yük dağılımında, ortalama 4.8-15 nm boyutlarda ve küresel görünümde oldukları bulgusuna ulaşıldı. Elde edilen AuNP'lerin ve AgNP'lerin, in-vitro antimikrobiyal ve antikanser etkileri, sırasıyla mikrodilüsyon ve MTT metotları kullanılarak incelendi. Sentezlenen AuNP'lerin antibiyotiklerden daha düşük olan, 0.01 ve 0.50 µg mL-1 aralığındaki konsantrasyonların patojen mikroorganizmaların üremelerinin baskılamada etkili oldukları görüldü. AuNP'lerin 25 µg mL-1 konsantrasyonunun en iyi antikanser etkisi, % 88.58 oranında baskılama ile CaCo-2 hücre hattı üzerinde olduğu belirlendi. Diğer hücre hatlarının üremesi üzerinde de etkili baskılama oluştuğu tespit edildi. AgNP'lerin patojen suşların üremesi üzerindeki baskılayıcı etkisinin 0.03-1.00 µg mL-1 gibi düşük konsantrasyonlarda, standart antibiyotiklerden daha etkili oldukları görüldü. AgNP'lerin sağlıklı hücre hattı HDF üzerinde 25 µg mL-1 konsantrasyonun % 79.45 canlılık oranı ile toksik etkisinin nerdeyse olmadığı belirlendi. Kanser hücre hatlarında ise canlılığı baskılamada en iyi etkinin, % 75.36 baskılama oranıyla CaCo-2 hücre hattı üzerinde oluşturduğu belirlendi. Bunun yanında diğer kanser hücreler hatlarının üremesi üzerinde önemli düzeyde baskılama meydana getirdi. C. tournefortii özütünün fitokimyasal bileşen bakımından karakterize edilmesi için, LC-MS/MS verilerinden elde edilen profil kullanıldı. Profilde en yüksek konsantrasyona sahip olan bileşenlerin sırasıyla, rutin, kumarin, biosiyanin A, şikimik asit, klorojenik asit olduğu belirlendi. Ayrıca özütün antimikrobiyal ve antikanser etkileri de incelendi. Özütün, AgNP'ler ve AuNP'lere kıyasla daha yüksek konsantrasyonda daha az etki gösterdiği belirlendi.
Composition and Characterization of Phytochemicals in Aromatic Species
(Elsevier, 2025) Baran, Ayşe; Güneş, Zübeyir; Department of Medical and Aromatic Plants / Tıbbi ve Aromatik Bitkiler Bölümü
Plants have a crucial role in sustaining all living organisms. Plants possess unique systems that they have evolved to counteract different biotic and abiotic stressors, ensuring the continuation of their essential functions. Specifically, the organisms generate secondary metabolites that bear significant bioactivity. Aromatic plants, rich in bioactive compounds, produce these phytochemicals as a defense mechanism and for preventive purposes, exerting substantial impacts on human health. The effects encompass antibacterial, anticancer, antioxidant, antidiabetic, and antiinflammatory actions. Incorporating plants with these effects into the human diet enhances the significance of plants in terms of their defensive and beneficial characteristics. The characterization of bioactive compounds in aromatic plants can be determined utilizing a variety of equipment and methodologies. By analyzing the chemical composition of the plant, we can establish the specific type and quantity of bioactive compounds it contains. These characterization techniques play a crucial role in determining the direction of many activities involving bioactive compounds. This study aimed to investigate the secondary metabolites, bioactivities, and characteristics of aromatic plants. © 2025 Elsevier Inc. All rights reserved.
Cytotoxic Potential of Selenium Nanoparticles (senps) Derived From Leaf Extract of Mentha Longifolia L.
(2024) Hatipoğlu, Abdulkerim; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü
The search for alternative methods in cancer treatments has been going on for many years. In the current study conducted for this purpose, selenium nanoparticles (ML-SeNPs) were produced from the aqueous leaf extract of Mentha longifolia L. easily and inexpensively, without harming the environment. The anticancer potential of ML-SeNPs on glioblastoma cell (U373), osteosarcoma cell (U2OS), and healthy retinal pigment epithelial cell (RPE-1) lines was determined by MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid) test. For the test, ML-SeNPs were applied at 100, 300, and 600 µg/mL levels and interaction was provided for 24 and 48 hours. The survival rates (%) in RPE-1, U373, and U2OS cell lines in the 24-hour application were 107.49-98.89, 97.66-86.82, and 87.81-83.37, respectively. The viability rates (%) of the cells in 48 hours of application were 72.27-87.39, 68.17-73.48, and 81.00-84.67, respectively. In general, it was discovered that the cytotoxic effect of ML-SeNPs on RPE-1, U373, and U2OS cell lines was greater at low doses and increased over time. In-vivo studies that support the antiproliferative action of ML-SeNPs may boost the prospect of using them as therapeutic agents in potential cancer treatment procedures in the following years.
Citation - WoS: 1
Citation - Scopus: 1
Deciphering the Effect Of Potentilla Fulgens Root Extract Against Healthy Huvec Cell Line and Cancer Cell Lines (a549 and Skov-3)
(Elsevier, 2024) Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Firat; Eftekhari, Aziz; Khusro, Ameer; Ommati, Mohammad Mehdi; Almutairi, Saeedah Musaed; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Background: Potentilla fulgens, , a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose- dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) P <0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-kappa B after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
Destructive Effect of Heavy Metals on the Oxidative Defense Mechanism of Some Aromatic Plants
(Elsevier, 2025) Baran, Ayşe; Yildiztekin, M.; Baran, A.
Aromatic plants are used in the production of natural medicines, treatment of various diseases, production of essential oils, and as a food source. Many plants are cultivated for their aromatic compounds. The essential oils they produce are used as antibacterial, antifungal, antiviral, and insecticidal agents, and as appetite suppressants against herbivores. Heavy metal pollution, which has become a significant environmental issue, can be highly reactive and toxic to living cells. Aromatic plants may be exposed to heavy metals in both natural and cultivated environments, leading to stress and oxidative damage in the plants. Some heavy metals cannot undergo biological degradation reactions, while others, due to their immobile nature, cannot be removed from the environment, resulting in accumulation. Those that are mobile can enter cells through various transport pathways and negatively affect physiological processes in the plant. The formation of reactive oxygen species (ROS) occurs as a result of these physiological processes. ROS types, such as O2 •-, 1O2, H2O2, and •OH, can be hazardous to cells, cause structural damage, and lead to cell death. The imbalance between the accumulation and removal of these oxidative molecules in the cell causes oxidative stress. The effects of oxidative stress can be observed in enzyme inactivation, disruption of membrane integrity, and inhibition of growth in plants. To mitigate the effects of oxidative stress and defend themselves, plants have antioxidant defense mechanisms. These mechanisms include enzymatic antioxidants such as superoxide dismutase, peroxidase, ascorbate peroxidase, and nonenzymatic antioxidants such as lipid-soluble and water-soluble reducing agents. The type, dose, and duration of heavy metal exposure, as well as the species and developmental stages of the plant, trigger various antioxidant defense mechanisms and responses. In this process, the role of ROS detoxifying enzymes is particularly significant. Due to their various properties, such as metal exclusion, aromatic plants are able to cope with oxidative stress. In conclusion, understanding the factors that affect the antioxidant defense mechanisms and enzyme inhibition in plants under heavy metal stress, and developing strategies to address them, is important for enhancing plant productivity and ensuring adaptation to environmental stress. © 2025 Elsevier Inc. All rights reserved.
Citation - WoS: 6
Citation - Scopus: 6
Determination of Chemical Components of the Endemic Species allium Turcicum L. Plant Extract by Lc-ms/Ms and Evaluation of Medicinal Potentials
(Cell Press, 2024) Baran, Ayşe; Baran, Mehmet Fırat; Baran, Ayse; Baran, Mehmet Firat; Ommati, Mohammad Mehdi; Karadag, Musa; Khalilov, Rovshan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU -145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 mu g/mL and 2000 mu g/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU -145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU -145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00 - 8.00 mu g/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies.