Browsing by Author "Baran, Ayşe"

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Adsorption of Crystal Violet Dye With Selenium Nanoparticles Obtained by Green Synthesis From Cherry (prunus Avium L.) Fruit Stalk
(2024) Solmaz, Alper; Turna, Talip; Baran, Ayşe
The rapid development of the global production printing and dyeing industry has led to an increase in the demand for various dyes. Crystal violet (CV), a versatile dye, is widely used in the textile industry and other applications. The reason for its widespread use is its effectiveness and the vivid color it gives to fabrics.CV dye is a water-soluble, toxic, resistant organic dye that is quite dangerous for the ecosystem and causes environmental pollution. Therefore, it must be removed before being released into the recipient environment. This study synthesized selenium nanoparticles (Se NPs) from agricultural Prunus avium L. (PaL.) wastes and removed CV dye. In batch adsorption tests, the effects of pH, amount of adsorbent, time, initial concentration, and temperature were investigated. In this study, where 3 different kinetic and isotherm models were tested, it was determined that the most suitable kinetic and isotherm models for the removal of CV dye with PaL-Se NPs were Pseudo second order (R2:0.999) and Langmuir (R2:0.997), respectively. Additionally, the maximum adsorption capacity (qmax) was calculated as 142.61 mgCV/g PaL-Se NP. Accordingly, it can be said that low-cost PaL-Se NPs synthesized by environmentally friendly methods are a suitable alternative for the removal of CV dye.
Adsorption of nickel(II) ions from aqueous solutions using Malatya clay: Equilibrium, kinetic, and thermodynamic studies
(Environmental Progress & Sustainable Energy, 2023) Baran, Ayşe; Dal, Mehmet Can; Onursal, Nilgün; Altunkaynak, Yalçın
This paper evaluates Malatya clay (MC) for the removal of Ni(II) ions from aqueous solutions. For adsorption efficiency, the impacts of several factors, such as pH, starting metal ion concentration, and contact duration, were examined. The optimal initial Ni(II) ion concentration was determined to be 5 mg/L, the adsorption contact duration was 360 min, and the solution's pH was 6.27. Using scanning electron microscopy, energy dispersive spectroscopy, and Fourier transform infrared spectroscopy, the surface characteristics of Malatya clay were examined. The isotherm data fit the Langmuir isotherm model, indicating chemical adsorption and likely irreversibility of the process, according to the results. At 298, 308, and 318 K, the adsorption capacities of Ni(II) were determined to be 10.267, 11.834, and 12.285 mg/g, respectively. Adsorption kinetic data revealed that the adsorption of Ni(II) metal ions followed the pseudosecond-order kinetic model. According to thermodynamic studies, Ni(II) ion adsorption on the MC is spontaneous and endothermic. MC has proven to be an effective and alternative material for the removal of Ni(II) ions from aqueous media due to its excellent removal ability, availability, and low cost, as well as its features such as being able to be used without any chemical treatment and being environmentally friendly.
Amygdalus Communis (badem) Meyvesinden Elde Edilen Özütlerin Bazı İn Vitro Biyolojik Aktivitelerinin, Kimyasal ve Pestisit Kompozisyonlarının Araştırılması
(2024) Keskin, Cumali; Aras, İbrahim Oğuz; Baran, Ayşe; Baran, Mehmet Fırat; Yıldız, Songül Çetik; Tarhan, Rozerin
Amygdalus communis (A. communis) yüksek düzeyde protein ve doymamış yağ asitlerinden oluşur ve bu da onları doğal kaynakların sınırlı olması gereken diyet beslenmesinde faydalı kılar. Bu çalışmada badem meyvesinden elde edilen kloroform ve metanol özütlerinin antioksidan, antikolinesteraz, antimikrobiyal özellikleri, kimyasal bileşen miktarı ile ağır metal kompozisyonu ve pestisit kalıntı miktarları araştırıldı. A. communis meyvesinde en yüksek toplam fenolik bileşen ve flavonoid içeriğin kloroform özütünde olduğu ayrıca kloroform özütünün DPPH radikal söndürme aktivitesinin daha yüksek olduğu belirlendi. A. communis meyve kloroform ve metanol özütlerinin Escherichia coli ve Staphylococcus aureus bakteri suşları ve Candida albicans mayasının üremesi üzerinde standart antibiyotikler ile kıyaslandığında düşük konsantrasyonlarda oldukça güçlü inhibisyona sebep olduğu belirlendi. A. communis meyve metanol ve kloroform özütlerinin kimyasal bileşen içerikleri LCMS/MS kullanılarak belirlendi. Metanol (Me-OH) özütünde ana bileşenlerin sırasıyla sitrik asit, şikorik asit ve 4-Hidroksibenzoik asit kloroform özütünde ise 4-Hidroksibenzoik asit, p-kumarik asit ve klorogenik asit olduğu belirlendi. A. communis meyve numunesi için pestisit kalıntı analizi GC-MS ve LCMS-MS kullanılarak yapıldı. Test edilen numune içeriğinde Deltametrin kalıntısının varlığı belirlendi. Elde edilen veriler değerlendirildiğinde A. communis meyvesinin zengin biyokimyasal içeriği ve güçlü antibakteriyel, antioksidan ve antikolinesteraz enzim inhibisyon kapasitesi ile biyomedikal ve gıda endüstrilerinde potansiyel uygulamalara sahip olması beklenmelidir.
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Anticancer Activities of Zinc Oxide Nanoparticles (znonps) Synthesized From Mentha Longifolia L. Leaf Extract
(2024) Hatipoğlu, Abdulkerim; İpek, Polat; Baran, Mehmet Fırat; Baran, Ayşe
Recently, there has been a remarkable increase in cancer and cancer-related deaths. In this study, the impacts of zinc oxide nanoparticles (ZnONPs) produced from the aqueous leaf extract of Mentha longifolia L. (ML) on ovary adenocarcinoma (OVCAR-3), colorectal carcinoma (HCT-116), and healthy retinal pigment epithelial cell (RPE-1) lines were investigated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was performed to discover its antiproliferative properties. As a result of the application of ML-ZnONPs on RPE-1, OVCAR-3, and HCT-116 cell lines at doses (µg/mL) of 250, 500, and 1000 for 24 hours, the viability rates (%) in the cell lines were 18.73-30.56, 21.98-28.76, and 27.27-40.93, respectively. In the 48-hour application, the viability rates (%) of the same cells were between 29.51-46.83, 32.49-40.81, and 46.82-44.37, respectively. The MTT test revealed that ML-ZnONPs strongly suppressed the growth of RPE-1, OVCAR-3, and HCT-116 cell lines. The test showed that the effect of dose increase and time on the viability of both cancer cell lines was negative.
Artemisia Absinthium Bitki Ekstraktından Altın Nanopartiküllerin Çevre Dostu Hızlı Sentezi ve Antimikrobiyal Aktivitelerin Uygulanması
(2021) Baran, Mehmet Fırat; Keskin, Cumali; Atalar, Mehmet Nuri; Baran, Ayşe
Nanopartiküller, üstün özelliklerinden dolayı birçok alanda kullanılmaktadır. Son zamanlarda, bu parçacıkların çevre dostu sentez yöntemleri kullanılarak üretilmesi çok popüler hale gelmiştir. Bu bağlamda, bu çalışmada, altın nanopartiküller (AuNP'ler), Artemisia absinthium'un (pelin otu) yeşil yaprak özütü ile hızlı ve çevre dostu bir yöntem kullanılarak sentezlenmiştir. AuNP'lerin tane boyutu Debye-Scherrer denklemine göre 13.40 nm olarak belirlendi. AuNP'lerin test edilen mikroorganizmaların büyümesi üzerindeki inhibe edici etkileri, minimum inhibe edici konsantrasyon (MİC) yöntemi ile belirlenmiştir. MİC, bir mikroorganizmanın büyümesini engelleyen bir antimikrobiyal maddenin minimum konsantrasyonu olarak tanımlanmaktadır. Elde edilen AuNP'ler, sırasıyla 0.250, 0.125, 0.125, 0.033 ve 0.66 mg mL-1 konsantrasyonlarında S. aureus, S. pyogenes, E. coli, P. aeruginosa suşları ve C. albicans mayası üzerinde önleyici etkiler göstermiştir.
Biochemical components, enzyme inhibitory, antioxidant and antimicrobial activities in endemic plant Scilla mesopotamica speta
(Wiley, 2021) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Akmeşe, Şükrü
In this study, in vitro antioxidant, antimicrobial, anticholinesterase and phenolic profile of different solvent extracts of Scilla mesopotamica speta were determined in detail. In vitro antioxidant activities and total phenolic and flavonoid contents of plant extracts obtained with different solvents were tested in terms of 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities. The highest total phenolic and total flavonoid contents were determined in the ethyl acetate extract (62.24 mu g GAE/mg) and chloroform extract (87.72 mu g QE/mg) respectively. The highest DPPH radical scavenging activity was detected in ethyl acetate extracts. Antimicrobial and antifungal activities were investigated by MIC method. The inhibitory activities of the extracts on the acetyl cholinesterase enzyme were investigated. Liquid chromatography (LC) tandem mass spectrometry LC-MS/MS was used to determine the phenolic component content of extracts. Thirty-one different components were identified in the analyses and their amounts were measured. Practical applications Scilla mesopotamica speta is an endemic and medicinal plant. It was determined that the extracts of this plant had a very rich content in terms of phenolic compounds, especially caffeic and ferulic acids. However, this plant was remarkable for its antioxidant, anticholinesterase, and antimicrobial activities. Considering the strong antioxidant, antimicrobial, and enzyme inhibition activities of the Scilla mesopotamica speta it can be suggested as a source of anticancer, antimicrobial, and antiviral drugs.
Biosynthesis of AgNPs by extract from waste leaves of Citrullus lanatus sp. (watermelon); characterization, antibacterial and antifungal effects
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe
Silver nanoparticles (AgNPs) are valuable materials with a large number of sectors used. Green synthesis is very important for biomedical applications as they show biocompatible properties. In this study, AgNPs were easily synthesized using the environmentally friendly green synthesis approach using agricultural waste parts of Citrullus lanatus sp. plant grown in Diyarbakır region. Characterization of synthesized AgNPs was made. Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to evaluate the phytochemicals responsible for effective reduction in the formation of AgNPs. UV-visible spectrophotometer (UV-Vis.) Spectra were also used to determine the presence of AgNPs. X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope, Zeta potential analyzes were performed to define the crystal structures, dimensions and surface charges of AgNPs, respectively. In these data, it was determined that AgNPs showed maximum absorbance at 460 nm, spherical appearance, 21.27 crystal nano size and -30.05 mV zeta potential. Antimicrobial effects of AgNPs on gram positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) bacteria, gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) bacteria, as well as on fungus C. albicans pathogenic microorganisms It was analyzed by specifying the Minimum Inhibition Concentration (MIC) by microdilution.
Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract
(Elsevier, 2023) Xu, Jiujun; Yıldıztekin, Mahmut; Han, Dayong; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Aytuğ Ava, Canan; İrtegün Kandemir, Sevgi; Cebe, Deniz Barış; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan
Metallic nanoparticle biosynthesis is thought to offer opportunities for a wide range of biological uses. The green process of turning biological waste into utilizable products gaining attention due to its economical and eco-friendly approach in recent years. This study reported the ability of Solanum tuberosum (ST) peel extract to the green synthesis of non-toxic, stable, small-sized silver nanoparticles without any toxic reducing agent utilizing the phytochemical components present in its structure. UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, flourier scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and energy dispersive analysis X-ray confirmed the biosynthesis and char-acterization of silver nanoparticles. Also, dynamic light scattering and thermogravimetric ana-lyses showed stable synthesized nanoparticles. The antibacterial activity of the biosynthesized silver nanoparticles was evaluated against four different bacterial strains, Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) Bacillus subtilis (B. subtilis), and a yeast, Candida albicans (C. albicans) using the minimum inhibitory concen-tration technique. The cytotoxic activities were determined against Human dermal fibroblast (HDF), glioblastoma (U118), colorectal adenocarcinoma (CaCo-2), and human ovarian (Skov-3) cell lines cancer cells using MTT test. The nanoparticle capping agents that could be involved in the reduction of silver ions to Ag NPs and their stabilization was identified using FTIR. Nano -particles were spherical in shape and had a size ranging from 3.91 to 27.07 nm, showed crys-talline nature, good stability (-31.3 mV), and the presence of capping agents. ST-Ag NPs significantly decreased the growth of bacterial strains after treatment. The in vitro analysis showed that the ST-Ag NPs demonstrated dose-dependent cytotoxicity against cell lines. Based on the data, it is feasible to infer that biogenic Ag NPs were capped with functional groups and demonstrated considerable potential as antibacterial and anticancer agents for biomedical and industrial applications.
Celtis Tournefortii Lam Yaprak Özütünün Bileşenlerinin Lc-ms/ms Aracılığıyla Belirlenmesi, Enzim İnhibisyonunun, Antimikrobiyal ve Antikanser Etkilerinin Araştırılması
(2023) Baran, Ayşe; Keskin, Cumali
Bitkilerden elde edilen özütlerde bulunan fitokimyasallar çok önemli biyoaktif bileşenlerdir. Bu çalışmada, Celtis tournefortii Lam. ağacı (CT) yapraklarından elde edilen özüt içeriğindeki fitokimyasallar LC-MS yöntemi ile belirlendi. Rutin (2479.89 µg ml-1), kumarin (1241.68 µg ml-1), biyokanin A (1026.42 µg ml-1), şikimik asit (477.32 µg ml-1) ve klorojenik asit (300.76 µg ml-1) bileşenlerinin yüksek konsantrasyonlara sahip olduğu belirlendi. CT ekstraktının patojen suşların büyümesi üzerinde baskılayıcı etkileri mikrodilüsyon yöntemi ile çalışıldı. 2.00-8.00 µg ml-1 konsantrasyon aralığında suşlar üzerinde baskılamaya neden olduğu gözlendi. Özütün asetil kolinesteraz ve glutatyon-S transferaz enzim aktiviteleri üzerindeki inhibisyon etkileri incelendi ve enzim aktivitesinin %50 inhibitör değerleri sırasıyla 13.58 ve 13.86 olarak bulundu. 3-(4,5-dimetiltiazol-2-il)-2,5-difenil tetrazol bromür tekniği uygulanarak CT yaprak özütünün hücre canlılığını baskılayıcı etkileri yumurtalık, kolon ve beyin kanseri hücrelerinin yanı sıra sağlıklı cilt fibroblast hücrelerinde çalışıldı. Kanser hücrelerinde sırasıyla %42, %4.27 ve %14.29 oranında baskılama oluşturduğu gözlendi.
Celtis Tournefortii Lam. Bitkisinin Yeşil Yapraklarından Gümüş ve Altın Nanopartiküllerin Biyolojik Yolla Sentezi, Karakterizasyonu, Kimyasal Kompozisyon ve İn-vitro Antimikrobiyal ve Sitotoksik Aktivitelerinin Belirlenmesi
(2024) Baran, Ayşe; Keskin, Cumali
Celtis tournefortii Lam. (C.tournefortii) bitkisinin atık durumundaki yeşil yapraklarından elde edilen özüt kullanılarak iki farklı konsantrasyonda altın nanopartiküllerin (AuNP'lerin) ve gümüş nanopartiküllerin (AgNP'lerin) sentezi, ucuz, kolay ve çevre dostu bir yaklaşımla yapıldı. Sentez sonucunda elde edilen AuNP'lerin ve AgNP'lerin karakterizasyonu UV-vis Spektrofotometre, XRD, FTIR, TEM, AFM, FESEM, EDX, TGA-DTA, DLS cihazlarından elde edilen veriler aracılığıyla değerlendirildi. 5 ve 10 mM'da sentezlenen AuNP'lerin sırasıyla, küresel morfolojik görünümde, negatif yüzey yük dağılımında, 553.67-555.32 nm maksimum absorbansa sahip ve 31.30-53.55 nm boyut dağılımında oldukları belirlendi. 5 ve 10 mM'da sentezlenen AgNP'lerin sırasıyla, 479.10-482.13 nm maksimum absorbansta, negatif yüzey yük dağılımında, ortalama 4.8-15 nm boyutlarda ve küresel görünümde oldukları bulgusuna ulaşıldı. Elde edilen AuNP'lerin ve AgNP'lerin, in-vitro antimikrobiyal ve antikanser etkileri, sırasıyla mikrodilüsyon ve MTT metotları kullanılarak incelendi. Sentezlenen AuNP'lerin antibiyotiklerden daha düşük olan, 0.01 ve 0.50 µg mL-1 aralığındaki konsantrasyonların patojen mikroorganizmaların üremelerinin baskılamada etkili oldukları görüldü. AuNP'lerin 25 µg mL-1 konsantrasyonunun en iyi antikanser etkisi, % 88.58 oranında baskılama ile CaCo-2 hücre hattı üzerinde olduğu belirlendi. Diğer hücre hatlarının üremesi üzerinde de etkili baskılama oluştuğu tespit edildi. AgNP'lerin patojen suşların üremesi üzerindeki baskılayıcı etkisinin 0.03-1.00 µg mL-1 gibi düşük konsantrasyonlarda, standart antibiyotiklerden daha etkili oldukları görüldü. AgNP'lerin sağlıklı hücre hattı HDF üzerinde 25 µg mL-1 konsantrasyonun % 79.45 canlılık oranı ile toksik etkisinin nerdeyse olmadığı belirlendi. Kanser hücre hatlarında ise canlılığı baskılamada en iyi etkinin, % 75.36 baskılama oranıyla CaCo-2 hücre hattı üzerinde oluşturduğu belirlendi. Bunun yanında diğer kanser hücreler hatlarının üremesi üzerinde önemli düzeyde baskılama meydana getirdi. C. tournefortii özütünün fitokimyasal bileşen bakımından karakterize edilmesi için, LC-MS/MS verilerinden elde edilen profil kullanıldı. Profilde en yüksek konsantrasyona sahip olan bileşenlerin sırasıyla, rutin, kumarin, biosiyanin A, şikimik asit, klorojenik asit olduğu belirlendi. Ayrıca özütün antimikrobiyal ve antikanser etkileri de incelendi. Özütün, AgNP'ler ve AuNP'lere kıyasla daha yüksek konsantrasyonda daha az etki gösterdiği belirlendi.
Cytotoxic Potential of Selenium Nanoparticles (senps) Derived From Leaf Extract of Mentha Longifolia L.
(2024) İpek, Polat; Baran, Ayşe; Hatipoğlu, Abdulkerim; Baran, Mehmet Fırat
The search for alternative methods in cancer treatments has been going on for many years. In the current study conducted for this purpose, selenium nanoparticles (ML-SeNPs) were produced from the aqueous leaf extract of Mentha longifolia L. easily and inexpensively, without harming the environment. The anticancer potential of ML-SeNPs on glioblastoma cell (U373), osteosarcoma cell (U2OS), and healthy retinal pigment epithelial cell (RPE-1) lines was determined by MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid) test. For the test, ML-SeNPs were applied at 100, 300, and 600 µg/mL levels and interaction was provided for 24 and 48 hours. The survival rates (%) in RPE-1, U373, and U2OS cell lines in the 24-hour application were 107.49-98.89, 97.66-86.82, and 87.81-83.37, respectively. The viability rates (%) of the cells in 48 hours of application were 72.27-87.39, 68.17-73.48, and 81.00-84.67, respectively. In general, it was discovered that the cytotoxic effect of ML-SeNPs on RPE-1, U373, and U2OS cell lines was greater at low doses and increased over time. In-vivo studies that support the antiproliferative action of ML-SeNPs may boost the prospect of using them as therapeutic agents in potential cancer treatment procedures in the following years.
Determination of Volatile Organic Compounds in Different Parts of Hibiscus Syriacus L. by Spme/Gc-ms Method
(2024) Koyuncu, Mubin; Karadağ, Musa; Baran, Ayşe; Güneş, Zübeyir
Aromatic plants are plants with a wide range of traditional uses. One of the areas of medical use is their consumption for nutritional purposes. Aromatic plants have been used for thousands of years for their preservative and medicinal properties and for enhancing the aroma and flavor of foods. Hibiscus species L. are among the aromatic plants widely used in Türkiye. Studies have shown that Hibiscus species L. have antiinflammatory, antibacterial, antifertility, antifungal, antioxidative, antihypertensive, and hypoglycaemic effects. The use of aromatic plants for therapeutic purposes for centuries has made their compounds the research subject. Gas chromatography-mass spectroscopy (GC-MS) was combined with solid phase microextraction (SPME) to determine volatile organic compounds. Twenty-nine volatile organic compounds were determined from different parts of H. syriacus L. species. The compounds with the highest content are carvacrol, thymol, eucalyptol, and linalool. The compounds with the highest ratios determined in the flower, seeds, leaves, and buds of Hibiscus syriacus L. were carvacrol (57.93 %), carvacrol (31.50 %), thymol (44.53 %) and carvacrol (54.54 %), respectively.
Ecofriendly Synthesis of Silver Nanoparticles Using Ananas comosus Fruit Peels: Anticancer and Antimicrobial Activities
(Hindawi, 2021) Baran, Ayşe; Keskin, Cumali; Baran, Mehmet Fırat; Huseynova, Irada; Khalilov, Rovshan; Eftekhari, Aziz; Irtegun-Kandemir, Sevgi; Kavak, Deniz Evrim
Metallic nanoparticles are valuable materials and have a range of uses. Nanoparticles synthesized from plant wastes by environment-friendly methods have attracted the attention of researchers in recent years. Also, the advantages of biological resources and synthesis methods are attracting attention. In this study, silver nanoparticles were synthesized from Ananas comosus fruit peels using ecofriendly method steps. The characterization of the particles obtained was determined by using a UV-visible spectrophotometer (UV-Vis.), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), Fourier scanning electron microscope (FESEM), and transmission electron microscopy (TEM). The nanoparticles showed maximum absorbance at 463 nm, measuring 11.61 in crystal nanosize, and presented spherical in appearance. An antimicrobial activity test was determined with the minimum inhibition concentration (MIC) method. The nanoparticles showed promising inhibitory activity on the Gram-positive and Gram-negative pathogen microorganisms (Escherichia coli ATCC25922, Staphylococcus aureus ATCC29213, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 bacteria, and Candida albicans yeast) at low concentrations. The cytotoxic and growth inhibitory effects of silver nanoparticles on different cancer cell lines were examined via the MTT assay. © 2021 Ayşe Baran et al.
Ecofriendly/Rapid Synthesis of Silver Nanoparticles Using Extract of Waste Parts of Artichoke (Cynara scolymus L.) and Evaluation of their Cytotoxic and Antibacterial Activities
(Hindawi, 2021) Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Irtegun Kandemir, Sevgi; Valiyeva, Mahbuba; Mehraliyeva, Sevil; Khalilov, Rovshan; Eftekhari, Aziz
Recycling wastes and providing their use in useful fields attract attention every day. In our study, with the extract prepared from the parts of the Cynara scolymus L. (artichoke) plant that is not suitable for human consumption, silver nanoparticles were easily synthesized in an ec-friendly, energy-free way. Characterization of the obtained nanoparticles was done with a UV-visible spectrophotometer (UV-Vis.), fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), and zeta potential analysis data. In these data, it was determined that AgNPs have a maximum absorbance at 458.8 nm wavelength, a crystal nanosize of 28.78 nm, and a spherical appearance. The zeta potential of (-) 16.9 mV indicates that silver nanoparticles exhibit a stable structure. Particles show antimicrobial effects on pathogenic species at concentrations of 0.03-0.25 μg/ml, and it was determined by using the minimum inhibition concentration (MIC) microdilution method. By examining their cytotoxic effects on U118, CaCo-2, and Skov-3 cancer cell lines and healthy HDF cell lines by the MTT method, concentrations of inhibitive effects on survival were determined.
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Environmentally Friendly Rapid Green Synthesis of Senps Using Grapefruit (citrus Paradisi) Leaves Extract, and Their Antimicrobial Potential
(2024) Baran, Ayşe
The utilisation of plant biomass in the production of nanoparticles is gaining popularity because of its associated benefits. Selenium nanoparticles (SeNPs) are highly valuable due to their involvement in numerous biological functions. In this study, SeNPs were rapidly synthesized using the environmentally friendly and low-cost green synthesis approach using Citrus paradisi (Grapefruit) leaves extract. The synthesized SeNPs were characterized using TEM, AFM, DLS, UV-vis, XRD, and EDX data. The data revealed that SeNPs had a spherical and uniform shape, with an average size of 45 nm, a surface charge of -20.54 mV, and a peak absorbance wavelength of 326 nm. The inhibitory impact of SeNPs on harmful strains and cancer cells was investigated using the microdilution method. The development of bacteria was effectively inhibited at concentrations ranging from 4 to 16 μg /ml.
Farklı Biyosorbentler Kullanarak Su Ortamında Ağır Metallerin Biyosorbsiyon Metodu ile Giderilmesi
(2022) Düz, M.zahir; Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe
Su canlılar için ekosistemin önemli parçasıdır. Endüstrileşme ile birlikte su kaynaklarındaki kirlenme endişe edilir boyutlara ulaşmıştır. Ağır metallere bağlı su kirliliği ve artan konsantrasyonları su ekosistemlerine verdiği zarar nedeniyle araştırmacıların konuya olan ilgisinin artmasına sebep olmuştur. Ağır metallerin sularda oluşturduğu kirliliği gidermek ciddi maliyet ve zaman gerektirmektedir. Son yıllarda suda bulunan ağır metallerin uzaklaştırılması için bakteriler kullanılarak biyosorbsiyon yönteminin kullanılması yaygınlaşmıştır. Bu yöntemin tercih edilmesinin temel sebebi gram pozitif bakterilerin hücre duvarında kalın bir peptidoglikan tabakasına sahip olması ile adsorbsiyon kapasitesini artırmasıdır. Bu çalışmada içme, atık, nehir suları ve suni olarak hazırlanan numunelerde çalkalamalı metod kullanarak ağır metal biyosorbsiyonu ile birlikte çoklu hazırlanmış ağır metal çözeltilerinde biyosorbsiyon rekabeti incelenmiştir. Bu işlemler için Dicle nehri bölgesine ait topraklardan izole edilen Bacillus licheniformis sp. Bacillus subtilis sp. ve Bacillus subtilis (ATCC 6051) suşları ile sulu çözeltiden Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metallerinin biyosorbsiyonu değerlendirildi. B. subtilis suşları ve B.licheniformis sp. organizmalarının yüzey morfoljik yapıları SEM görüntüleri, element kompozisyonları EDAX verileri ile incelendi. ICP-OES kullanılarak element içeriği tespit edildi. Sulu çözeltideki Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metal iyonları farklı pH’larda 25 °C de 0,25 mg L-1 biomass ile biyosorbsiyon gerçekleştirildi. pH 6.0 da maksimum biyosorbsiyon ile metal iyonlarının % 98 varan oranda giderildiği belirlendi.
Fast and Economical Biosorption of Lead (II) Heavy Metal in Aqueous Solutions by Bacillus licheniformis sp.
(Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Baran, Mehmet Fırat; Düz, Zahir; Baran, Ayşe; Keskin, Cumali
Lead is used in many areas of industry. Considering the prevalence of use, there is an intense lead and heavy metal content in the wastes resulting from these applications, and the toxic pollution caused by these metals affects the nearest water source directly or indirectly. Lead poses a serious threat to all life forms in the ecosystem, even at low concentrations in water. Lead recovery with environmentally friendly methods is both easier and more economical. Bacillus licheniformis sp. type was used as a biosorbent in the study. Nearly 98.4% of lead was removed by using the batch biosorption method, at 25 oC, pH 5.5, with an adsorption capacity of 42.92 in 120 minutes. from the water. The properties of the biosorbent, such as its morphological appearance, were characterized by scanning electron microscopy (SEM). Besides, the functional groups affecting biosorption in the surface structure were investigated by fourier transform Infrared spectroscopy (FT-IR)while its resistance to heat treatment was measured by thermal gravimetric analysis (TGA-DTA). The lead(II) element content in the aqueous solution was also determined by inductively coupled plasma optical emission spectroscopy (ICP-OES).
Fast and Low-Cost Biosynthesis of AgNPs with Almond Leaves: Medical Applications with Biocompatible Structures
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe
In this study, silver nanoparticles (AgNPs) were obtained in a low cost, easy and simple way by using the leaf extract of Prunus dulcis L. (almond tree) growing in the Mardin region. Characterization of AgNPs obtained by biosynthesis: Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), UV-visible spectrophotometer (UV-Vis.), Scanning Electron Microscope (SEM), Transmission Electron Microscope(TEM), Zeta potential and Zeta size analysis was done through data. It was determined that AgNPs have maximum absorbance at 443 nm wavelength, they exhibit 14.67 crystal nano size, -19.9 mV zeta potential in spherical appearance. The Minimum Inhibition Concentration (MIC) of the obtained AgNPs was determined by using microdilution method on the growth of pathogen strains.