Browsing by Author "Baran, Mehmet Firat"

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Citation - WoS: 3
Citation - Scopus: 6
Anticancer, Antioxidant and Antimicrobial Activity of Elaeagnus Angustifolia L. Leaf Extract
(Dove Medical Press Ltd, 2025) Do Gan, Serap; Mese, Ahmet; Baran, Mehmet Firat; Baran, Ayse; Aktepe, Necmettin; Ahmadian, Elham; Aktas, Husnu; Doğan, Serap
Objective: This study was conducted to determine and compare the antioxidant, cytotoxic, and antimicrobial effects of spindle leaves of Elaeagnus angustifolia L. (E. angustifolia) (oleaster) leaves. Methods: Total phenolic content was measured using the Folin-Ciocalteu method, phenolic compound analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) and antimicrobial effect by the minimum inhibition concentration (MIC) method. The free radical scavenging activity was determined by the (2,2-Diphenyl-1-picrylhydrazyl) DPPH method, the free radical scavenging activity was determined by the ABTS method, and cytotoxicity assays were performed by the MTT method in human retinal epithelium cells (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer cells (DU-145) cell lines. Results: High amounts of gallic acid, protocatechuic acid, and o-coumaric acid were identified as phenolic compounds. E. angustifolia was found to have a good antioxidant capacity and high free radical scavenging capacity. In this study, for the first time, E. angustifolia leaf extract was used to investigate cytotoxic effects on human retinal epithelium (RPE-1), human osteosarcoma cells (U2OS), and prostate cancer (DU-145) cells and antimicrobial effects on Listeria monocytogenes American Type Culture Collection (ATTC) 7644, Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 11774, and Candida albicans ATCC 10231 microorganisms. The highest cytotoxic effect was observed in the DU-145 cell line, and the highest antimicrobial effect was observed in Listeria monocytogenes ATTC 7644 and Candida albicans ATCC 10231. The leaf extract of the plant contains some important phenolic compounds and has high free radical scavenging capacity, a good anticancer effect, and effective antimicrobial activity on yeast species such as C. albicans. Conclusion: Our study will contribute greatly to the search for anticancer and antimicrobial agents, especially from a pharmacological perspective, by examining biological activity using three different methods.
Citation - WoS: 2
Citation - Scopus: 2
Antioxidant Properties of allium Turcicum Özhatay & Cowley Plant Extract, Its Effects on the Proliferation and Migration of Cancer Cells
(Frontiers Media Sa, 2024) Ipek, Polat; Baran, Ayse; Cebe, Deniz Baris; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Firat
Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum & Ouml;zhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum & Ouml;zhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 mu g/mL, respectively. We conducted a cell migration experiment to study the A. turcicum & Ouml;zhatay & Cowley Extract (AT & Ouml;CE) impact on cancer cells' metastatic behavior. Our findings indicate that AT & Ouml;CE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of AT & Ouml;CE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 +/- 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 +/- 0.044 mg GAE/g.
Citation - WoS: 28
Citation - Scopus: 30
A Comprehensive Analysis Of Arum Dioscoridis Plant Leaf Extract as a Corrosion Inhibitor for Mild Steel in 1 M Hcl: Synthesis, Characterization, Surface Analysis Observations, Experimental and Dft Studies
(Elsevier, 2025) Doner, Ali; Yildiz, Resit; Arslanhan, Selim; Baran, Mehmet Firat
Background: Corrosion occurs wherever metal and its alloys exist. Protection of metals with corrosion inhibitor is a popular topic. Considering environmental concerns and human health, it is more favorable to use green corrosion inhibitors than traditional corrosion inhibitors. Methanol extract of Arum dioscoridis (AD) becomes a potential green corrosion inhibitor and it can be used in industrial areas. Methods: Corrosion efficiency, corrosion behavior and corrosion mechanism of Arum dioscoridis leaf extract on mild steel (MS) are illuminated in 1 M HCl by Tafel curves, electrochemical impedance spectroscopy (EIS), linear polarization resistance (LPR), zero charge potential, structural and surface morphological analysis and density functional theory (DFT). Significant findings: The extract function as mixed-type corrosion inhibitor with predominantly cathodic action. Inhibition efficiency was reached the value of 97 %. Polarization resistance is measured as 761 Omega cm2 at 1000 ppm of AD. The high inhibition efficiency was attributed to phytochemicals in the AD extract. A high activation energy (70.34 kJ/mol) for inhibited solution than that of in blank solution (49.66 kJ/mol). Both physisorption and chemisorption are responsible for formation of a protective layer on MS surface to inhibit the electrochemical reactions. Optimized molecular structures in phytochemicals confirmed the inhibitive properties via DFT.
Citation - WoS: 2
Citation - Scopus: 2
Deciphering the Effect Of Potentilla Fulgens Root Extract Against Healthy Huvec Cell Line and Cancer Cell Lines (a549 and Skov-3)
(Elsevier, 2024) Ipek, Polat; Baran, Ayse; Baran, Mehmet Firat; Eftekhari, Aziz; Khusro, Ameer; Ommati, Mohammad Mehdi; Almutairi, Saeedah Musaed; Fırat Baran, Mehmet; Mehdi Ommati, Mohammad; Musaed Almutairi, Saeedah
Background: Potentilla fulgens, , a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose- dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) P <0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-kappa B after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
Citation - WoS: 22
Citation - Scopus: 25
Determination of Antimicrobial and Toxic Metal Removal Activities of Plant-Based Synthesized (capsicum Annuum L. Leaves), Ecofriendly, Gold Nanomaterials
(Wiley-v C H verlag Gmbh, 2020) Baran, Mehmet Firat; Acay, Hilal; Keskin, Cumali
Nanoparticles are valuable materials with widespread use. The fact that these materials are obtained by biological resources with an environmentally friendly method contributes to the development of studies in this field. Gold nanoparticles (AuNPs) from waste vegetable sources (green leaves of Capsicum annum L.) are economically and easily synthesized. The obtained particles are characterized by UV-vis spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analysis. The antimicrobial activity of the particles on the pathogenic microorganisms Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, Bacillus subtilis bacteria, and Candida albicans yeast are found to have a significant suppressive effect. The removal activities of eight toxic metals (Pd, Cd, Fe, Ni, Co, Mn, Zn, Pb) in Diyarbakir drinking water and artificially prepared water within different pHs are investigated. Gold nanoparticles synthesized from Capsicum annuum L. leaves are found to be effective in toxic metal removal in water samples.
Citation - WoS: 8
Citation - Scopus: 10
Determination of Chemical Components of the Endemic Species allium Turcicum L. Plant Extract by Lc-ms/Ms and Evaluation of Medicinal Potentials
(Cell Press, 2024) Ipek, Polat; Atalar, Mehmet Nuri; Baran, Ayse; Baran, Mehmet Firat; Ommati, Mohammad Mehdi; Karadag, Musa; Khalilov, Rovshan
The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU -145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 mu g/mL and 2000 mu g/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU -145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU -145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00 - 8.00 mu g/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies.
Citation - WoS: 3
Citation - Scopus: 4
Determination of Chemical Composition and Antioxidant, Cytotoxic, Antimicrobial, and Enzyme Inhibition Activities of Rumex Acetosella L. Plant Extract
(Springer int Publ AG, 2024) Kandemir, Sevgi Irtegun; Aktepe, Necmettin; Baran, Ayse; Baran, Mehmet Firat; Atalar, Mehmet Nuri; Keskin, Cumali; Khalilov, Rovshan; Irtegün Kandemir, Sevgi
Purpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.
Citation - WoS: 19
Citation - Scopus: 20
Green synthesis and characterization of selenium nanoparticles (Se NPs) from the skin (testa) of Pistacia vera L. (Siirt pistachio) and investigation of antimicrobial and anticancer potentials
(SpringerLink, 2023) Keskin, Cumali; Mehmet Fırat Baran, Cumali Keskin, Ayşe Baran, Kadri Kurt, Polat İpek, Aziz Eftekhari, Rovshan Khalilov, Ismayil Fridunbayov, William C. Cho; Baran, Mehmet Firat; Eftekhari, Aziz; Kurt, Kadri; Baran, Ayse; Ipek, Polat; Cho, William C.
Metallic nanoparticles created by ecologically friendly synthesis processes are becoming increasingly useful in a variety of applications. Because of their strong bioactive component qualities, biocompatible architectures, high stability, and low toxicity, green-produced selenium nanoparticles are particularly signifcant materials for various medicinal applications. Plants include a wealth of essential phytochemicals with therapeutic and medical capabilities. Pistachio vera L. (Siirt pistachio) is a seasonal fruit that is frequently consumed for its nutritional worth and health advantages. However, the outer colored skin of the P. vera (Siirt pistachio) fruit, which is not consumed, contains many biologically active compounds. In this study, plant-mediated synthesis of selenium nanoparticles (SeNPs) was successfully accomplished after adding the sodium selenite solution to the aqueous extract of P. vera colored skin waste. The synthesized Se NPs were characterized with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta distribution, atomic force microscope (AFM), Fourier transform infrared spectrum (FTIR), thermogravimetric (TGA) and diferential thermal analysis, X-ray difraction (XRD), and energy-dispersive X-ray spectrum (EDX). Biogenic Pistacia vera (PV) PV-SeNPs were also tested for their ability to suppress the development of various pathogenic bacteria and cancerous cell lines. The UV-visible (UV–Vis) spectra revealed an absorption peak at 325 nm, which refected the surface plasmon band. The signifcant selenium signal on the EDX spectrum at 1.5 keV confrmed the creation of Se NPs. The presence of several peaks on the FTIR spectrum of the aqueous extract of Pv and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols, which are important in facilitating the process of capping and bioreduction, as well as conferring stability to nanoparticles. The TEM microphotographs revealed that the nanoparticles were highly distributed, had a spherical morphological form, and were monodisperse below 10 nm. Biogenic Pv-SeNPs exhibited similar antimicrobial activity as standard antibiotics. However, it was determined that the cytotoxic activity of Se-NPs against cancer cell lines was quite high depending on the dose and time. As a result, the Pv-SeNPs are likely to be extremely benefcial in the pharmaceutical and cosmetic sectors, as well as in the food and cosmetic industries, in producing antimicrobial and/or anticancer medications.
Citation - WoS: 27
Citation - Scopus: 33
Green Synthesis and Characterization of Silver Nanoparticles Using Anchusa Officinalis: Antimicrobial and Cytotoxic Potential
(Dove Medical Press Ltd, 2025) Keskin, Cumali; Aslan, Seyhan; Baran, Mehmet Firat; Baran, Ayse; Eftekhari, Aziz; Adican, Mehmet Tevfik; Mohamed, Ali Jimale
Objective: Anchusa officinalis L. (A. officinalis) is a herbaceous traditional medicinal plant used in the treatment of some diseases. The presence of its medicinal properties suggested that A. officinalis (AO) leaf extract could be used as a coating agent for the environmentally friendly production of silver nanoparticles (AgNPs). Methods: The synthesized biogenic silver nanoparticles (AO-AgNPs) were characterized using different techniques. The antimicrobial activity of AgNPs against common bacterial pathogenic strains was determined by the minimum inhibitory concentration (MIC) method. The presence of phytochemicals was determined by LSMS/MS. The MTT assay was used to investigate AO-AgNPs' cytotoxic activity in malignant (LnCap, Caco2, MDA-MB2, A549) and healthy (HEK-293) cell lines. Results: LC-MS/MS analysis detected the presence of rich phytochemicals that may be responsible for reduction reactions. Biogenic AO-AgNPs exhibited effective inhibition of the growth of pathogenic microorganisms at low concentrations. The most effective antimicrobial activity was measured as 0.5 mu g/mL MIC against S. aureus, E. coli, and C. albicans. Moreover, AO-AgNPs showed significant inhibition on the growth of cancerous cell lines, especially at a concentration of 25 mu g/mL. On the contrary, it was determined that the inhibition rate decreased in the growth of healthy cell lines due to the increase in concentration. The lowest EC50 values were determined as 15.15 mu g/mL in A549 cells. Conclusion: The obtained results showed that AO could be an important source for the synthesis of AgNPs. Especially their ability to inhibit the growth of antibiotic-resistant pathogenic bacteria at low concentrations compared to common antibiotics indicates that AOAgNPs can be used as biomedical agents in various areas. Moreover, their suppressive effect on cancerous cell lines showed that they have the potential to be used as an anticancer agent, but due to their proliferative effect on healthy cell lines, care should be taken in determining the appropriate dose.
Citation - WoS: 56
Citation - Scopus: 62
Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract
(SpringerLink, 2023) Baran, Ayşe; Polat İpek, Mehmet Fırat Baran, Ayşe Baran, Abdulkerim Hatipoğlu, Cumali Keskin, Mahmut Yildiztekin, Selçuk Küçükaydin, Hatice Becerekli, Kadri Kurt, Aziz Eftekhari, Irada Huseynova, Rovshan Khalilov, William C. Cho; Baran, Mehmet Firat; Yildiztekin, Mahmut; Keskin, Cumali; Hatipoglu, Abdulkerim; Ipek, Polat; Cho, William C.
Gold nanoparticles (Au NPs) have potentially therapeutic properties as they are synthesized via biomolecules as reducing and stabilizing agent(s). The aim of this study is to develop an easy and eco-friendly method for the synthesis of Au NPs using extracts from the Allium cepa (AC) red peel (skin) extract and investigate the antibacterial and antioxidant activity and also inhibitory efects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. UV–Vis peak at~564 nm confrmed the Au NPs absorbance. TEM images revealed the formation of Au NPs with mostly spherical shapes and sizes between 6.08 and 54.20 nm. FTIR analysis confrmed the important biological compounds responsible for the reduction of gold. The strong absorption property of Au NPs was studied by EDX. The produced Au NPs demonstrated signifcant antibacterial and antifungal activity against the bacterial and fungal strains tested, as well as efcient inhibitory activity against both AChE and BChE enzymes. The highest antimicrobial activities were found against Staphylococcus aureus (0.06° mg/ ml) and Candida albicans (0.06° mg/ml). The antioxidant test fndings revealed that AC-Au NPs had lesser activity when compared to normal antioxidants. The Au NPs showed excellent inhibitory efcacy against AChE and BChE. The proposed green technique could encourage the innocuous generation of Au NPs, implying therapeutic possibilities.
Citation - WoS: 18
Citation - Scopus: 23
Green synthesis of silver nanoparticles based on the Raphanus sativus leaf aqueous extract and their toxicological/microbiological activities
(SpringerLink, 2023) Hatipoğlu, Abdulkerim; Ayşe Baran, Cumali Keskin, Mehmet Fırat Baran, Aziz Eftekhari, Sabina Omarova, Dawid Janas, Rovshan Khalilov, Mehmet Tevfk Adican, Sevgi İrtegün Kandemir; Baran, Mehmet Firat; Eftekhari, Aziz; Keskin, Cumali; Baran, Ayse; Omarova, Sabina; Kandemir, Sevgi Irtegun
Silver nanoparticles (AgNPs) have several uses. Many scientists are working on producing AgNPs from plant extracts for use as biomedicines against drug-resistant bacteria and malignant cell lines. In the current study, plant-based AgNPs were synthesized using Raphanus sativus L. (RS) leaf aqua extract. Diferent concentrations of AgNO3 were used to optimize the synthesis process of RS-AgNPs from the aqueous leaf extract. Energy-dispersive X-ray analysis (EDX), transmission electron microscopy (TEM), scanning electron microscopy (SEM), atomic force microscope (AFM), and UV–vis spectroscopy were used to analyze the generated materials. Furthermore, to evaluate the biological properties of the obtained materials, Bacillus subtilis (B. subtilis), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. albicans) pathogen strains were used for the minimum inhibitory concentration (MIC) assays. Subsequently, healthy cell lines (human dermal fbroblast (HDF)) and cancerous cell lines (glioma/U118, Ovarian/ Skov-3, and colorectal adenocarcinoma/CaCo-2) were engaged to determine the cytotoxic efects of the synthesized NPs. The cytotoxic and anti-pathogenic potential of AgNPs synthesized by the proposed green approach was investigated. The results were encouraging compared to the standards and other controls. Plant-based AgNPs were found to be potential therapeutic agents against the human colon cancer cell (CaCo-2) and showed strong inhibitory activity on Candida albicans and Staphylococcus aureus growth. The RS-AgNPs generated have highly efective antimicrobial properties against pathogenic bacteria. Our fndings also show that green RS-AgNPs are more cytotoxic against cancerous cell lines than normal cell lines. Synthesized nanoparticles with desirable morphology and ease of preparation are thought to be promising materials for antimicrobial, cytotoxic, and catalytic applications.
Green Synthesis of Silver Nanoparticles Derived from Papaver Rhoeas L. Leaf Extract: Cytotoxic and Antimicrobial Properties
(MDPI, 2023) Baran, Mehmet Firat; Beylerli, Ozal; Baran, Ayse; Sufianov, Albert; Yildiz, Resit; Hatipoglu, Abdulkerim; Ipek, Polat
In the last few decades, the search for metal nanoparticles as an alternative to cancer treatments and antibiotics has increased. In this article, the spectroscopic (ultraviolet-visible (UV-vis), electron-dispersing X-ray (EDX), and Fourier transform infrared (FT-IR)), microscopic (field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), and atomic force microscope (AFM)), structural (X-ray diffractometer (XRD) and zetasizer), and analytic (thermogravimetric/differential thermal analyzer (TGA-DTA)) characterization of the silver nanoparticles (AgNPs) produced from Papaver rhoeas (PR) L. leaf extract are presented. PR-AgNPs are generally spherical and have a maximum surface plasmon resonance of 464.03 nm. The dimensions of the manufactured nanomaterial are in the range of 1.47-7.31 nm. PR-AgNPs have high thermal stability and a zeta potential of 36.1 mV. The minimum inhibitory concentration (MIC) values (mg L-1) of PR-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans are 1.50, 0.75, 3.00, 6.00, and 0.37, respectively. In the study, the cytotoxic and proliferative effects of PR-AgNPs using the MTT (3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide) method on various cancer cell lines (CACO-2 (human colon adenocarcinoma cell), MCF-7 (human breast cancer cell), T98-G (glioblastoma multiforme cell), and healthy HUVEC (human umbilical vein endothelial cell)) cell lines are presented. After 24 and 48 h of the application, the half-maximum inhibitory concentration (IC50) values (mu g mL(-1)) of PR-AgNPs on HUVEC, CACO-2, MCF-7, and T98-G lines are 2.365 and 2.380; 2.526 and 2.521; 3.274 and 3.318; 3.472 and 3.526, respectively. Comprehensive in vivo research of PR-AgNPs is proposed to reveal their potential for usage in sectors such as nanomedicine and nanochemistry.
Citation - WoS: 103
Citation - Scopus: 125
Green Synthesis of Silver Nanoparticles from Allium cepa L. Peel Extract, Their Antioxidant, Antipathogenic, and Anticholinesterase Activity
(Molecules, 2023) Keskin, Cumali; Mehmet Fırat Baran, Ayşe Baran, Abdulkerim Hatipoğlu, Mahmut Yildiztekin, Selçuk Küçükaydin, Kadri Kurt, Hülya Hoşgören. Moklesur Rahman Sarker, Albert Sufianov, Ozal Beylerli, Rovshan Khalilov, Aziz Eftekhari; Baran, Mehmet Firat; Yildiztekin, Mahmut; Kucukaydin, Selcuk; Eftekhari, Aziz; Hatipoglu, Abdulkerim; Baran, Ayse
The present work deals with the green synthesis and characterization of silver nanoparticles (AgNPs) using Allium cepa (yellowish peel) and the evaluation of its antimicrobial, antioxidant, and anticholinesterase activities. For the synthesis of AgNPs, peel aqueous extract (200 mL) was treated with a 40 mM AgNO3 solution (200 mL) at room temperature, and a color change was observed. In UV-Visible spectroscopy, an absorption peak formation at ~439 nm was the sign that AgNPs were present in the reaction solution. UV-vis, FE-SEM, TEM, EDX, AFM, XRD, TG/DT analyses, and Zetasizer techniques were used to characterize the biosynthesized nanoparticles. The crystal average size and zeta potential of AC-AgNPs with predominantly spherical shapes were measured as 19.47 ± 1.12 nm and −13.1 mV, respectively. Pathogenic microorganisms Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans were used for the Minimum Inhibition Concentration (MIC) test. When compared to tested standard antibiotics, AC-AgNPs demonstrated good growth inhibitory activities on P. aeuruginosa, B. subtilis, and S. aureus strains. In vitro, the antioxidant properties of AC-AgNPs were measured using different spectrophotometric techniques. In the β-Carotene linoleic acid lipid peroxidation assay, AC-AgNPs showed the strongest antioxidant activity with an IC50 value of 116.9 µg/mL, followed by metal-chelating capacity and ABTS cation radical scavenging activity with IC50 values of 120.4 µg/mL and 128.5 µg/mL, respectively. The inhibitory effects of produced AgNPs on the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were determined using spectrophotometric techniques. This study provides an eco-friendly, inexpensive, and easy method for the synthesis of AgNPs that can be used for biomedical activities and also has other possible industrial applications.
Citation - WoS: 16
Citation - Scopus: 22
Green-Synthesized Characterization, Antioxidant and Antibacterial Applications of Ctac/Mnps-ag Nanocomposites
(Mdpi, 2024) Baran, Ayse; Ertas, Erdal; Baran, Mehmet Firat; Eftekhari, Aziz; Gunes, Zubeyir; Keskin, Cumali; Khalilov, Rovshan
The emergence of antibiotic resistance, caused by the improper use of antibiotics, is a significant challenge in combating infectious diseases, leading to millions of annual fatalities. The occurrence of antimicrobial side effects catalyzes the investigation of novel antimicrobial compounds and sources of drugs. Consequently, the research on biological activity that is conducted on plants, plant extracts, and compounds that are produced from plant components is of utmost significance. In this study, CtAC/MNPs were obtained by the reaction of activated carbon (AC) obtained from the fruits of the Celtis tournefortii (Ct) plant and magnetic nanoparticles (MNPs), and a CtAC/MNPs-Ag nanocomposite was synthesized by the reduction in silver ions added to the reaction. The synthesized CtAC/MNPs and CtAC/MNPs-Ag nanocomposites were analyzed spectroscopically (FTIR, XRD), microscopically (SEM, EDX), optically (DLS), electrochemically (zeta potential) and magnetically (VSM). The antibacterial activities of CtAC/MNPs and CtAC/MNPs-Ag nanocomposites against S. aureus and E. coli were investigated by microdilution method using minimal inhibitory concentration (MIC) and disk diffusion methods. Antioxidant activity study, including total phenolic content and DPPH and cuprac assays, revealed the remarkable effect of the CtAC/MNPs-Ag nanocomposite. This study has the advantages of obtaining CtAC/MNPs and CtAC/MNPs-Ag nanocomposites in a short time without requiring energy, and most importantly, the reaction takes place without using any toxic substances. In addition, according to the data obtained in the study, the CtAC/MNPs-Ag nanocomposite is thought to shed light on biomedical research.
Citation - WoS: 12
Citation - Scopus: 13
The Investigation of the Chemical Composition and Applicability of Gold Nanoparticles Synthesized with Amygdalus communis (Almond) Leaf Aqueous Extract as Antimicrobial and Anticancer Agents
(Molecules, 2023) Keskin, Cumali; Mehmet Fırat Baran, Cumali Keskin, Ayşe Baran, Aziz Eftekhari, Sabina Omarova, Rovshan Khalilov, Mehmet Tevfik Adican, Gvozden Rosić, Dragica Selakovic, Mahmut Yıldıztekin, Kadri Kurt, Canan Aytuğ Ava, Mehmet Nuri Atalar; Baran, Mehmet Firat; Atalar, Mehmet Nuri; Eftekhari, Aziz; Khalilov, Rovshan; Baran, Ayse; Omarova, Sabina
The current work’s main objective was to determine the chemical composition of Amygdalus communis (AC) leaf extract and examine the antibacterial and cytotoxic properties of biosynthesized gold nanoparticles (AuNPs). The chemical composition of AC leaf extract was determined using LC-ESI/MS/MS to detect compounds that may be responsible for the reducing, stabilizing, and capping steps in the synthesis of nanoparticles and their biological activities. The AC-AuNPs were spherical, with a particle size lower than 100 nm and a face-centered cubic structure. The EDX spectrum confirmed the formation of AuNPs and a negative zeta potential value (−27.7 mV) suggested their physicochemical stability. The in vitro cytotoxic efficacy of the AC-AuNPs against colorectal adenocarcinoma (Caco-2), glioma (U118), and ovarian (Skov-3) cancer cell lines and human dermal fibroblasts (HDFs) was evaluated by MTT assay. CaCo-2 cell proliferation was effectively inhibited by the AC-AuNPs at concentrations between 25 and 100 g mL−1. The AC-AuNPs exerted preeminent antimicrobial activity against Bacillus subtilis with an MIC of 0.02 μg/mL, whilst good activity was shown against Staphylococcus aureus bacteria and Candida albicans yeast with an MIC of 0.12 μg/mL. Ultimately, the results support the high antibacterial and anticancer potential of biosynthesized AuNPs from AC leaf extract.
Investigation of the Effect of Cuscuta Spp. Extract on Cell Viability
(2025) Öziç, Cem; Baran, Ayşe; Aktepe, Necmettin; Güneş, Zübeyir; Baran, Mehmet Firat
Cancer is becoming an increasingly serious health problem worldwide. While various treatment approaches exist for diseases like colorectal cancer, they have not yet been found to be fully effective. Therefore, the development of new anti-cancer drugs is crucial. Cuscuta is a plant used in functional foods and for traditional medicinal purposes in various cultures, including Ayurveda and its medicinal traditions, which are widespread throughout the world. In preclinical studies, Cuscuta spp. have demonstrated anticytotoxic and cytoprotective properties and potential anticancer effects, particularly in various cell lines and animal models. Fibroblasts are scientifically proven to be the primary normal cell type of connective tissue, typically mesenchymal in origin. This is represented by the laboratory L929 mouse fibroblast cell line. Colorectal cancer is the third most common cancer worldwide and the third most common cause of cancer-related death. Our study aimed to determine the cytotoxic effects of Cuscuta spp extract, known to have medicinal effects in various diseases in public health, on the L929 mouse fibroblast cell line and the HT-29 human colorectal adenocarcinoma cell line. For cytotoxic analysis, the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] enzymatic test was preferred. In vitro testing using Cuscuta spp extract revealed decreased viability in L929 fibroblast cell line and HT-29 human colorectal adenocarcinoma cells exposed to Cuscuta spp plant extract for 24 and 48 hours. The plant extract showed more cytotoxic effects on fibroblast cells, a normal cell line. In conclusion, this study demonstrates that Cuscuta spp. extract exhibits high cytotoxicity on normal cells and has limited effects on cancer cells. Further studies are needed for its pharmacological use.
Citation - WoS: 17
Citation - Scopus: 23
lavandula Angustifolia Extract as a Green Corrosion Inhibitor for Protection of Mild Steel in Hcl Acid Solution
(Maik Nauka/interperiodica/springer, 2024) Arslanhan, Selim; Sigircik, Gokmen; Yildiz, Resit; Baran, Mehmet Firat
A new Lavandula angustifolia extract was prepared to investigate the anti-corrosion effect against mild steel in corrosive environment. Inhibitory performance of green extract was examined in detail with electrochemical, morphological, as well as quantum chemical calculation analyses. The effects of inhibitor concentration and exposure time were examined to describe the inhibition behavior. Physical and chemical adsorption kinds happen between the surface of the steel and Lavandula angustifolia extract. Furthermore, surface analysis studies were applied to justify the formation of protective adsorption coating occurred on the surface of steel. Quantum chemical calculation results are also in good consistent with other experimental outcomes.
Citation - WoS: 2
Citation - Scopus: 2
Nano-Enabled Biochar Modulate Arsenic Toxicity in Plants: a Step Towards Crop Safety and Health
(Springer Int Publ Ag, 2025) Faizan, Mohammad; Sharma, Pooja; Eren, Abdullah; Afzal, Shadma; Alam, Pravej; Baran, Mehmet Firat; Hayat, Shamsul
As global agricultural demands continue to rise amidst increasing environmental stressors, enhancing plant resilience has become a critical necessity. Heavy metals (HMs), especially arsenic (As), severely impact crop productivity and quality, threatening global food security and human health. Arsenic toxicity disrupts normal physiological, biochemical and molecular processes in food crops due to its eco-toxicological effects. Nano-enabled biochar (Nano-BC) has emerged as a promising soil amendment capable of mitigating As-induced oxidative stress in horticultural crops by modulating stress responses, enhancing detoxification pathways and improving plant resilience. This review comprehensively examines the interactions of Nano-BC with soil matrices and microbial communities, highlighting its dual role in influencing soil health and plant growth. While Nano-BC improves soil structure, increases nutrient retention and supports beneficial microbial populations, its potential long-term ecological impacts and interactions with native microbial communities require further evaluation. Furthermore, we discuss the mechanisms by which Nano-BC modulates As bioavailability, phyto-toxicity and detoxification pathways in horticultural crops. Advances in Nano-BC applications have demonstrated its potential in producing "pollution-safe" crops, yet challenges remain regarding its environmental fate and persistence. Future research should focus on optimizing Nano-BC formulations to enhance its efficiency while minimizing unintended ecological consequences. By integrating Nano-BC into sustainable agricultural practices, we can move closer to achieving food security, mitigating HM stress in crops and aligning with global sustainable development goals (SDGs).
A New Green Corrosion Inhibitor for Mild Steel in 1 M HCl Using Erodium Cicutarium (L.) Leaf Extract: Insights From Both Theoretical and Experimental Methods
(Elsevier, 2026) Ergel, Busra; Yildiz, Resit; Arslanhan, Selim; Baran, Mehmet Firat
Considering the economic and environmental conditions, corrosion appears to be a significant handicap. In this study, anti-corrosion performance of Erodium cicutarium (L.) (EC) is explored in a 1.0 M HCl using potentiaodynamic polarization (PDP), electrochemical impedance (EIS) and linear polarization resistance (LPR) spectroscopies along with morphological, structural and quantum chemical methods. FT-IR and LC-ESI-MS/MS reveal the chlorogenic acid, gentisic acid, protocatechuic acid, vanillin and salicylic acid, containing-C=O, C=C, C-H, C-H, -OH, and aromatic ring. PDP, EIS and LPR showed that at the highest concentration of the EC extract (1000 ppm), the polarization resistance and corrosion current density are measured as 974 Omega cm2 and 246 mu A cm-2, while the inhibition efficiency was found to be 98 %. Inhibition efficiencies decreased with increasing temperature and exposure times due to desorption of adsorbed inhibitor molecules from the surface. But, a notable increase in the Ea value (67.2 kJ mol-1) is observed, thereby hindering the corrosion process. While adsorption behavior is in accordance with the Langmuir isotherm, the Delta G degrees ads (-27.34 kJ mol-1) value indicates both physical and chemical adsorptions. To better understand the inhibitory mechanism, zero charge potential is determined, and quantum chemical calculations of the EC extract's components are performed.
Preparation and Characterization of Silver-Loaded Magnetic Activated Carbon Produced From Crataegus Monogyna for Antimicrobial and Antioxidant Applications
(Wiley-v C H verlag Gmbh, 2025) Ertas, Erdal; Dogan, Serap; Baran, Ayse; Baran, Mehmet Firat; Evcil, Murat; Kurt, Baris; Aslan, Kadir Sinan
Secondary metabolites from several plant species have been used to cure various illnesses. Current advances allowed green synthesis nanoparticle manufacturing of metal salts from plant sources. This study involves binding activated carbon obtained from the Crataegus monogyna plant to magnetic nanoparticles and coating the resulting magnetic activated carbon nanocomposite with Ag ions (CMAC/MNPs-Ag) to produce a biomedical nanobiological material. Various techniques such as SEM, EDX, XRD, FTIR, UV-Vis, VSM, DLS, and zeta potential were used to characterize synthesized nanocomposites. CMAC/MNPs-Ag nanocomposite demonstrated activity in several processes of antioxidant activity tests. DPPH and CUPRAC activities of CMAC/MNPs-Ag nanocomposite were measured as 90.21 +/- 0.42 and 46.73 +/- 0.108 mg TE/g, respectively, while total phenolic content was measured as 27.15 +/- 0.381 mg GAE/g. Finally, the antibacterial activity of CMAC/MNPs-Ag nanocomposite was evaluated against Escherichia coli and Staphylococcus aureus by microdilution and disk diffusion techniques. The antimicrobial activity of CMAC/MNPs-Ag nanocomposite was determined using microdilution and disk diffusion techniques. For Escherichia coli, microdilution and disk diffusion were measured as 1.17 mu g mL-1 and 12 mm, respectively, while for Staphylococcus aureus, microdilution and disk diffusion were measured as 2.34 mu g mL-1 and 10 mm, respectively.