Beslenme ve Diyetetik Bölümü Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/223

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4,4'-Diaminobifenil’in Yumuşak Çeliğin Hidroklorik Asitteki Korozyonuna Karşı İnhibisyon Etkisi Ve Kuantum Hesaplamaları
(Fırat Üniversitesi Mühendislik Bilimleri Dergisi, 2018) Yıldız, Reşit; Dehri, İlyas
In this study, 4,4′-Diamınobıphenyl (4-DB) is investigated usage of inhibitor molecule. Electrochemical techniques (EIS and polarization measurements) and quantum chemical calculations ((DFT) with the B3LYP/6- 311++G (d, p)) were obtained. The results showed that; inhibition efficiencies increase with increasing concentration of 4-DB. It is found that 4-DB retards anodic reactions according to polarization measurements. The inhibition efficiency of 4-DB is attributed to the high value of EHOMO (–0,189 eV), dipole moment μ (0,6121 D) and low value of ELUMO (-0.019 eV). This inhibitor functions through adsorption following Langmuir isotherm. The value of adsorption free energy of (∆G°ads) was also calculated.
Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties
(Gültekin ÖZDEMİR, 2023) İpek, Polat; Baran, Mehmet Fırta; Yıldız, Reşit; Hatipoğlu, Abdulkerim
This study was carried out to synthesize silver nanoparticles (AgNPs) from Arum dioscoridis (AD) leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible (UV-vis) Spectroscopy, Field Emission Scanning Electron Microscopy (FE-SEM), Electron Dispersive X-ray (EDX) Spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffractometer (XRD), Atomic Force Microscopy (AFM) and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration (MIC) values (mg/L) of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid] assay, the half-maximal inhibitory concentrations (IC50: μg/mL) of AD-AgNPs on human colon adenocarcinoma cell (CACO-2), human breast cancer cell (MCF-7), glioblastoma multiforme cell (T98-G), and healthy human umbilical vein endothelial cell (HUVEC) lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.
Capoeta trutta’nın Kas ve Karaciğer Dokusu Total Lipid Fosfolipit ve Triaçilgliserol Yağ Asidi Kompozisyonu Capoeta trutta’nın yağ asitleri
(2017) Kaçar, Semra; Başhan, Mehmet
Bu çalışmada, Atatürk Baraj Gölü'ndeki Capoeta trutta'nın lipid içeriği ve total lipid, fosfolipit (PL), triaçilgliserol (TAG) yağ asidi profili incelendi. Major doymuş yağ asitleri (DYA) palmitik (C16:0) ve stearik (C18:0) asittir. Oleik asit (C18:1n-9) ve palmitoleik asit (C16:1n-7) belirgin tekli doymamış yağ asitleri (TDYA)'ndendir. Her iki dokuda dominant çoklu doymamış yağ asitleri(ÇDYA) eikosapentaenoik asit (EPA, C20:5n-3) ve dokosaheksaenoik (DHA, C22:6n-3) asittir. ?DYA, ?TDYA ve ?ÇDYA oranlarının dağılımları fosfolipit (PL) ve triaçilgliserol (TAG) fraksiyonları arasında farklı bulunmuştur. TAG, PL'ye oranla daha az oranda ?ÇDYA içerirken, daha fazla oranda ?TDYA ve ?DYA içermiştir. Kas dokusunda, n-3/n-6 oranı 5.95 bulunmuştur. C. trutta içerdiği n-3 yağ asitleri ve n-3/n-6 oranı ile ekonomik olarak önemlidir
Clinical Relevance of Preoperative Neutrophil to Lymphocyte and Platelet to Lymphocyte Ratio in Renal Cell Carcinoma
(Journal of Urological Surgery, 2018) Kölükçü, Engin; Kılıç, Şahin; Atılgan, Doğan; Parlaktaş, Bekir; Ceylan, Salih; Unsal, Velid; Etikan, İlker
Objective: The association of preoperative neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) with postoperative tumor stage and Fuhrman nuclear grade was investigated in patients with renal cell carcinoma (RCC). Materials and Methods: Data of 123 patients, who were operated due to RCC, in our clinic was analysed. NLR and PLR were evaluated in patients who were classified according to tumor stage (T1 and T2 low stage, T3 and T4 high stage) and Fuhrman nuclear grade (grade 1 and 2 low-grade, grade 3 and 4 high-grade). NLR and PLR were compared using Levene’s test between the groups. Results: Sixty four patients were female (52.1%) and 59 were male (47.9%). All haematological parameters were expressed as 103 /μL. Mean age, blood neutrophil, lymphocyte and platelet counts, and NLR and PLR values of the patients were 62.49±12.43 years, 6.27±2.8, 2.05±0.83, 263.72±89.03, 4.01±3.93, and 149.73±82.1, respectively. The most common histologic subtype was recorded as clear cell RCC (76.4%). NLR and PLR were 3.83±3.22 and 142.79±66.66, respectively in the low-stage group and 4.43±5.29 and 165.85±109.41, respectively in the high stage group. As for the Fuhrman nuclear grading, NLR and PLR were 3.81±3.45 and 146.63±87.36, respectively in the low-grade group and 4.61±5.387 and 159.32±63.42 in the high-grade group. There was no statistically significant difference between the groups (p>0.05). Conclusion: Although not statistically significant, high tumor stage and nuclear grade were positively correlated with NLR and PLR. It is concluded that, further multi-center and prospective studies with larger samples are needed to derive meaningful results.
Clinical significance of neutrophil-lymphocyte ratio in patients with low-risk prostate cancer
(Annals of Medical Research, 2021) Kölükçü, Engin; Ozbek, Latif Mustafa; Unsal, Velid
Aim: Active surveillance is a highly emphasized approach to low-risk prostate cancer. Upgrading and upstaging should be evaluated carefully in this strategic management. This study aimed to analyze the relationship of neutrophil-lymphocyte ratio (NLR) with these two clinical conditions. Materials and Methods: Demographic data, prostate-specific antigen (PSA) levels, prostate volumes, NLR, disease stages, and Gleason scores of 59 low-risk prostate cancer patients who underwent radical prostatectomy and met active surveillance criteria were evaluated in our clinic. The patients were examined in four groups according to their postoperative pathology specimens. Accordingly, the patients with upgrading Group 1, while those without formed Group 2. Similarly, Group 3 consisted of the cases with upstaging and the patients with no upstaging were determined as Group 4. Results: Median age, PSA levels, prostate volumes, neutrophil and lymphocyte counts of the patients were 69.0 (63.0-72.0) years, 7.24 (5.50-8.90) ng/dL, 65.0 (60.0-65.0) cc, 6.40 (4.87-8.73) K/uL, and 2.50 (1.60-3.10) K/uL, respectively. Prostate volume and age distribution were similar between the groups. PSA levels were higher in Group 1 and Group 3 than those in Group 2 and Group 4 (p=0.012 and p=0.049, respectively). NLR was 3.54 (1.89-5.45) and 1.94 (1.68-3.76) in groups 1 and 2, respectively. Although these values were low in Group 2, a statistically significant relationship could not be established (p=0.266). NLR in groups 3 and 4 was 2.46 (1.52-5.45) and 2.24 (1.68-4.35), respectively. The NLR level in Group 3 was high; however, the difference was not statistically significant (p=0.953). Conclusion: The study let us to conclude that NLR alone is not sufficient to predict the clinical course of patients with low-risk prostate cancer.
Citation - WoS: 1
Citation - Scopus: 2
Comparative Study of Lipid and Fatty Acid Profile in Liver Tissues of Male and Female Silurus triostegus During the Catching Seasons
(2021) Kaçar, Semra; Başhan, Mehmet
In this study, seasonal variations of total fatty acid (FA), phospholipid (PL) and triacylglycerol (TAG) compositions in liver tissues of catfish (Silurus triostegus) were investigated. Samples of S. triostegus were obtained from Atatürk Dam Lake, Turkey, in two month periods during one year as from May. The major components were palmitic acid (16:0), stearic acid (18:0), oleic acid (18:1n-9), palmi toleic acid (16:1n-7), docosahexaenoic acid (DHA, 22:6n-3) and arachidonic acid (AA, 20:4n-6) in total lipid, 16:0, 18:0, 18:1n-9, eicosapentaenoic acid (EPA, 20:5n-3), DHA and AA in PL, 16:0, 16:1n7, 18:1n-9, linoleic acid (LA, 18:2n-6), AA, EPA, DHA and docosapentaenoic acid (22:5n-3) in TAG extracted from the liver of S. triostegus in all seasons. N-3/n-6 ratio was found 2.00-2.61 in females, 1.15-2.75 in males in total lipid. The highest lipid content was found in May (2.39%) in the females. In the males, the highest level was found in September (2.91%). In TAG fraction, the component with the lowest ratio in both sexes’ TAG is PUFA. In PL fraction, SFA, MUFA, and PUFA percentages were found at similar rates in both sexes in all months.
CuZn Elektrotundan Zn’nin Uzaklaştırılmasının Hidrojen Çıkış Davranışı Üzerine Etkisi
(2021) Toprak Döşlü, Serap
Hidrojen üretiminde, elektrokimyasal yolla suyun ayrıştırılmasında yüksek katalitik aktiviteye sahip ve kararlı elektrotların geliştirilmesi çok önemlidir. Burada, CuZn elektrotunun fabrikasyonunu yapmak için ucuz alkali uzaklaştırma yöntemi kullanılmış ve bu yöntem elektrota, hidrojen oluşum reaksiyonu (HER) için yüksek yüzey alanı ve verimli aktif merkezler sağlamıştır. CuZn elektrotu karakterize etmek için, taramalı elektron mikroskop (SEM), enerji dağılımlı X-Işını (EDX), atomik kuvvet mikroskop (AFM) ve Dönüşümlü voltametri (CV) tekniklerinden yararlanılmıştır. CuZn elektrotunun hidrojen oluşum karakteristiği 1,0 M KOH çözeltisinde, bakır kaplı bakır elektrot (Cu/Cu) ile kıyaslanmıştır. SEM analizlerine göre, Cu/Cu elektrotta bakır nano-tanecikler yüzeye dağılırken, CuZn elektrotta karnabahara benzeyen şekiller elde edilmiştir. Metallerin karakteristik pikleri CV ve EDX spektrumlarında ortaya çıkmıştır. HER etkinlikleri değerlendirildiğinde, CuZn elektrot (0.309 mA cm-2 ), Cu/Cu elektrot (0.043 mA cm-2 ) ile kıyaslandığında yüksek yük değişimi akım yoğunluğu elde edilmiştir. Ayrıca, CuZn elektrot, 50 mA cm-2 akım yoğunluğunda daha düşük aşırı gerilime ve daha düşük Tafel eğimine sahiptir. Bu CuZn elektrotun Cu/Cu elektrotundan daha yüksek HER performansına sahip olduğu anlamına gelir. Bu yüksek katalitik aktivite, alkali uzaklaştırma nedeniyle yüksek yüzey alanı ve Zn ve Cu metallerinin sinerjistik etkisi ile açıklanabilir. Bu deneylere ek olarak, CuZn elektrot bazik ortamda 18000 saniye boyunca oldukça yüksek kararlılık ve dayanıklılık göstermiştir.
Cyprinus carpio Karaciğer Dokusu Yağ Asidi İçeriğinin Mevsimsel Değişimi
(Karadeniz Fen Bilimleri Dergisi, 2022) Kaçar, Semra; Başhan, Mehmet
Bu çalışmada, dişi ve erkek C. carpio’nun karaciğer dokusu yağ asidi bileşiminin mevsime bağlı değişimleri araştırılmıştır. Balıkların kas dokusu total lipit yağ asidi kompozisyonları; yağ asidi standartları kullanılarak tespit edilmiştir. Tüm mevsimlerde C. carpio’nun karaciğer dokusundaki total lipitlerde SFA (doymuş yağ asitleri) içinde temel bileşen 16:0, MUFA (tekli doymamış yağ asitleri) içinde 16:1n-7 ve 18:1n-9, PUFA (çoklu doymamış yağ asitleri) içinde EPA ve DHA olmuştur. Balığın karaciğer dokusundaki TAG (triaçilgliserol) ve PL (fosfolipid) yağ asidi içeriklerinin farklı olduğu bulunmuştur. Balıkların karaciğer dokusunda baskın yağ asitleri; üreme, sıcaklık ve mevsime bağlı olarak değişmiştir. C. carpio’nun her iki eşeyinin total lipitlerinde n-3/n-6 oranı; karaciğer dokusunda 1.33-2.55 aralığında tespit edilmiştir.
Determination of Antioxidant and cytotoxic activities of king oyster mushroom mediated AgNPs synthesized with environmentally friendly methods
(Medicine Science, 2020) Acay, Hilal; Baran, Mehmet Fırat
Synthesizing nanoparticles (NPs) using wild edible fungi with environmentally friendly synthesis methods is more preferred because of the advantages it provides. The fact that its synthesis is easy, economical, non-toxic and has a wide range of uses increases the interest in this subject. The aim of this study was to determine the antioxidant and cytotoxic activity of biomolecular synthesized Pleurotus eryngii silver nanoparticles (PE-AgNPs) against human prostate carcinoma (PC-3), human cervix (HeLa) and breast carcinoma (MCF-7) cells. PE-AgNPs showed significant cytotoxic activity against HeLa, PC-3, MCF-7 cell lines, and also dimethyl sulfoxide solvents of PE-AgNPs applied for their metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and antioxidant activity using the β-carotene linoleate model system. Biosynthetic PE-AgNPs were found to inhibit the proliferation of PC-3, HeLa and MCF-7 cells with IC50 values of 2.185, 46.594 and 6.169 µg / ml, respectively, during a 24-hour incubation period. With the parallel of increasing concentration (1, 2, 5, 10 mg/mL) the activities were also increased at all the tests studied. At 10 mg/ml antioxidant activities were 82%, 85% and 77% for chelation of ferrous ions reducing power, DPPH scavenging and β-carotene linoleate tests respectively. The results show that PE-AgNPs may contribute to the development of a suitable anticancer drug that can lead to a new development of nanoparticles for cancer treatment. It also appears to be advantageous to use nanotechnology and green chemistry to improve the existing therapeutic properties of P.eryngii.
Düşük Karbonlu Çeliğin Korozyon Davranışına Hekzametilen Tetra Amin’in Etkisi
(2017) Yıldız, Reşit
In this study, the inhibition effect of hexamethylene tetramin (HMTA) on low carbon steel corrosion behavior in acidic environment is investigated. The electrochemical techniques and quantum chemical calculations were obtained. The results showed that; inhibition efficiencies increase with increasing concentration of HMTA. The inhibition efficiency is 93,6% in 10 mM HMTA containing 0,1 M HCl solution. The high inhibition efficiency of HMTA is attributed to the high value of EHOMO (-6.203 eV) and low value of ELUMO (-0.346 eV). The adsorption of HMTA on mild steel was found to fit to Langmuir adsorption isotherm. The inhibition efficiency of HMTA was discussed in terms of adsorption of inhibitor molecules on the metal surface and a protective film formation.
The Effect of N-acetylcysteine on Amylase, Electrolytes, Vitamins and Nitrosative Stress Levels in Rats Treated with Maras Powder
(Eastern Journal of Medicine, 2020) Unsal, Velid; Belge Kurutaş, Ergül
The aim of this study is to investigate the effects of N-acetylcysteine (NAC) on amylase, electrolytes, vitamins and nitrosative stress levels in the plasma of rats treated with smokeless tobacco "maras powder". Our study consisted of three groups. Control (n = 10), the group using maras powder (n = 10), maras powder+ NAC group. To the Maras powder group, 200 mg maras powder was placed in the sublingual area under general anesthesia. It was waited for 15 minutes for Maras powder to be absorbed through mucosa. This procedure was repeated once a day and for 7 days. To the NAC group, 200 mg of Maras powder was given as in the Maras powder group and NAC was injected intraperitoneally with a dose of 100 mg / kg / day. On the 8th day, the levels of amylase, vitamins (A, C and E), electrolytes (Na+, K+, Cl- ) and, as the biomarkers of nitrosative stress, nitric oxide (NO) and nitrotyrosine (3-NTx) in the plasma of all three groups were measured by the methods of ELISA. It was determined that amylase, 3-NTx, NO, electrolyte levels increased in the group using Maras powder compared to the control and NAC groups, but vitamin levels decreased. (p <0.05). It was detected that the levels of amylase, 3-NTx, NO, electrolyte in the group treated with NAC decreased compared to the group using Maras powder, whereas vitamin levels significantly increased and approached to the values of the control group. Smokeless tobacco 'Maras powder' increases nitrosative stress and distorts the levels of vitamin A, E, C and electrolyte in the plasma. NAC has a positive function in reducing the damage caused by Maras powder.
Citation - WoS: 2
Citation - Scopus: 1
Effects of Ketamine on Penile Tissues in an Experimental Priapism Model in Rats
(Turkish Assoc Trauma Emergency Surgery, 2024) Gevrek, Fikret; Fırat, Fatih; Unsal, Velid; Tapar, Hakan; Karaman, Tugba; Karaman, Serkan; Yalçın, Kenan
BACKGROUND: This study aimed to evaluate the histopathological and biochemical effects of ketamine on penile tissues following ischemia-reperfusion injury induced by priapism. METHODS: Twenty-four male rats were randomized into three groups. Group 1 served as the control group. Group 2 underwent the priapism model to induce ischemia-reperfusion injury. Group 3, the treatment group, experienced a similar ischemia-reperfusion model as Group 2; additionally, 50 mg/kg of ketamine was administered intraperitoneally just before reperfusion. Blood biochemical analyses and penile histopathological evaluations were performed. RESULTS: In Group 3, significant improvements were observed in all histopathological scores, including desquamation, edema, inflammation, and vasocongestion compared to Group 2 (p<0.001). Blood biochemical analyses showed that the malondialdehyde (MDA) levels were recorded as 10 in Group 2, with a significant decrease in Group 3 (p=0.013). Similarly, proinflammatory cytokine levels, including interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α), were found to be suppressed in Group 3 compared to Group 2 (p=0.003, p=0.022, and p=0.028, respectively). Antioxidant enzyme activities, such as glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), were higher in Group 3 compared to Group 2 (p=0.016 and p=0.024, respec- tively). CONCLUSION: Ketamine is an effective anesthetic agent in alleviating the effects of penile ischemia-reperfusion injury.
Fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions of the wild edible mushroom pleurotus ostreatus and russula delica with cytotoxic activities on prostate carcinoma cell lines
(2019) Acay, Hilal; Baran, Mehmet Fırat
The aim of the study to investigate fatty acids (FAs) of Pleurotus ostreatus and Russula delica in Total Lipid (TL), Triacylglycerol (TG) and Phospholipid (PL) fractions. The major FAs of TL, TG, PL in both species were palmitic acid (PA), oleic acid (OLA), and linoleic acid (LA). In both species, total PUFA amounts were found to be higher than total monounsaturated fatty acids (MUFA) and total saturated fatty acids (SFA) in TL, TG and PL fractions. Also, insufficiently studied cytotoxic activity (using prostate carcinoma (PC-3) cell lines) of these mushrooms were investigated by using various solvent systems. Ethyl acetate extract of Pleurotus ostreatus and Russula delica showed significant inhibitory value at the concentrations of 520-530 μg/ml (99.45-92.82%) against PC-3 cell lines with the half-maximal inhibitory concentration (IC50); 274.53-297.77 μg/mL respectively. The present study is a guide for biochemical and nutritional values of both species and can be useful for further investigation on pharmacological applications.
Gümüş Nanopartiküllerin Yeşil Sentezi ve Bazı Gıda Patojenleri Üzerindeki Antimikrobiyal Etkileri
(Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2022) Hatipoğlu, Abdulkerim
Bu çalışmada, gümüş nanopartiküller (AgNP’ler) biyolojik yöntemle kolay, düşük maliyetli ve çevre dostu bir şekilde elde edilmiştir. Sentezi yapılan AgNP’lerin karakterizasyonu UV-visible Spektrofotometre (UV-Vis.), Alan Emisyon Taramalı Elektron Mikroskobu (FE-SEM), Transmisyon Elektron Mikroskobu (TEM), Enerji Dağılımlı X-Işını Spektroskopisi (EDX), X- Işını Kırınımı Difraktrometresi (XRD), Fourier Dönüşümü Kızılötesi Spektroskopisi (FT-IR) ve Zeta boyut ve potansiyel cihazı kullanılarak yapılmıştır. AgNP’lerin 440-450 nm absorbansta maksimum pik vermiştir. TEM ve FE-SEM sonuçlarına göre nanopartiküllerin morfolojik yapılarının küresel ve ortalama 38 nm; XRD sonuçlarına göre nanopartiküllerin toz kristal yapılarının kübik ve 21.94 nm boyutunda; zeta boyutunun ise ortalama 158.2 nm, zeta potansiyelinin -23.4 mV olduğu görülmüştür. Sentezlenen AgNP'lerin Staphylococcus aureus ATCC 29213, Bacillus subtilis ATCC 11774 Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 ve Candida albicans üzerinde Minimum İnhibisyon Konsantrasyonları (MİK) (mg/mL) sırasıyla 1.25, 0.625, 2.50, 1.25 ve 0.312 olarak tespit edilmiştir. Söz konusu mikroorganizmaların AgNO3 ve antibiyotik MİK değerleri (mg/mL) sırasıyla 2.65;2, 1.32;1, 0.66;2, 1.32;4, 0.66;2 olarak tespit edilmiştir.
Citation - WoS: 1
Investigation of Nutritional Content, Antioxidant Anticancer and Antimicrobial Activities of Pleurotus eryngii (DC. Ex Fr.) Quel, Extract Obtained by Different Solvents
(KSÜ Tarım ve Doğa Dergisi, 2021) Acay, Hilal; Dündar, Abdurrahman; Kaçar, Semra; Baran, Mehmet Fırat; Keskin, Cumali
The present study related the nutritional contents, fatty acidcompositions, in vitro antioxidant, anticancer and antimicrobialactivities of different solvents extracts of Pleurotus eryngii. Thenutritional contents of the P. eryngii were investigated in detail.Crude protein (g 100 kg-1), carbohydrate (g 100 kg-1), lipid (g 100 kg1), ash (g 100 kg-1), dietary fiber (g 100 kg-1) and energy value (cal100 g-1) of fruiting bodies were analysed. Fatty acid compositions oftotal lipid, phospholipid and triacylglycerol fractions of Pleurotuseryngii were investigated by Gas chromatography. Totally 13 differentfatty acids constituents were identified. Antioxidant propertiesincluding total antioxidant activity, free radical scavenging activity,reducing power activity, metal chelating activity, and superoxideanion radical scavenging activity were evaluated using different tests.The highest total antioxidant, DPPH scavenging, metal chelating, andsuperoxide anion radical scavenging activities were determined inethyl acetate extracts. The potential of cytotoxic activity on PC-3(prostate cancer) cell lines was found to depend on the concentrationand the type of the tested extracts fractions. It was determined thathexane, ethyl acetate, and methanol extracts of Pleurotus eryngii haveinhibition effect on the growth of tested microorganisms. The presentresults suggested that used as a daily nutrient, the Pleurotus eryngiicould be a novel source for new drug developments and promising insome cancer treatment.
Küçük Aile İşletmelerinde Üretilen Diyarbakır Örgü Peynirinin Bileşim ve Bazı Biyokimyasal Özellikleri
(Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 2021) Hatipoğlu, Abdulkerim; Çelik, Şerafettin
Bu çalışmada, Diyarbakır yöresinde peynir üreten küçük aile işletmelerinden taze ve olgun Diyarbakır Örgü peyniri (DÖP) örnekleri alınmış ve bileşim ve bazı biyokimyasal özellikleri araştırılmıştır. İstatistiksel değerlendirmede, taze ve olgun peynirlerin yağ, kurumaddede (KM’de) yağ, kül, tuz ve KM’de tuz oranları ile titrasyon asitliği (TA)pH 4.6’da çözünen azot (ÇA), pH ve lipoliz değerleri arasındaki farklılık önemli bulunmuştur. Türk Gıda Kodeksi Peynir Tebliği bağlamında, taze DÖP’ün yarı sert ve yarım yağlı, olgun peynirin ise sert ve az yağlı peynir sınıfında değerlendirilebileceği, KM’ de tuz oranlarının ise anılan tebliğde izin verilen orandan yüksek olduğu sonucuna varılmıştır. Küçük aile işletmelerinde üretilen DÖP’ün bileşimi standardize edilmeli, özellikle peynirin tuz oranı ilgili tebliğde izin verilen düzeye düşürülmelidir.
Citation - WoS: 6
Citation - Scopus: 5
The protective effects of sinapic acid on acute renal ischemia/reperfusion injury
(Turkish Journal of Biochemistry, 2021) Unsal, Velid; kolukcu, Engin; Firat, Fatih; Gevrek, Fikret
Objectives: The aim of this research was to investigate whether sinapic acid (SA) can alleviate oxidative damage, apoptosis, and inflammation in I/R induced renal injury. Methods: A total of 24 male rats were randomly separated into four groups as six rats in each group. Group 1 (Sham), Group 2 (I/R), Group 3 (I/R + SA, 10mg/kg), Group 4 (I/R + SA, 20 mg/kg). In order to evaluate kidney function serum BUN, Cr, and AST were measured in an autoanalyzer. SOD, GSH-Px, MDA, PC and NO oxidative stress parameters were measured with spectrophotometric methods and TNF-α, IL-1β, IL-6, KIM-1 and NGAL parameters were measured with the ELISA method. In addition, H&E method and immunohistochemical examinations were performed for histological evaluations of kidney tissue. Results: SA significantly decreases the increase in kidney damage, inflammation, oxidative stress, cell death and restore the decrease in antioxidant enzyme activities (p<0.05). Pre-treatment of the rats with SA reduces kidney dysfunction and morphological changes. Conclusions: The development of oxidative stress and lipid peroxidation seems to be the leading factors that accelerate inflammation and cell death during renal IRI. The antioxidant, anti-inflammatory, and anti-apoptotic features of SA displayed a renoprotective effect.
Relationship Between Lymph Node Metastasis and Lymph Node Density and Preoperative Neutrophil-Lymphocyte Ratio in Patients Undergoing Radical Cystectomy
(UROONKOLOJI BULTENI-BULLETIN OF UROONCOLOGY, 2021) Kölükçü, Engin; Atılgan, Doğan; Kuyucu, Yunus Emre; Unsal, Velid; Özbek, Latif Mustafa
Objective: This study aimed to evaluate the relationship between the preoperative neutrophil-lymphocyte ratio (NLR) and lymph node metastasis and lymph node density after radical cystectomy in patients with invasive urinary bladder cancer. Materials and Methods: Data of 89 patients who underwent radical cystectomy were examined. Our study included only cases with stage 2 urothelial bladder cancer. They were classified according to the lymph node status based on the surgical specimen. Patients with negative results were classified as group 1 and those with positive results as group 2. Patients in group 2 were further evaluated in two subgroups according to their lymph node density. Accordingly, group 2A consisted of patients with lymph node density of <20%, and group 2B involved those with lymph node density of ≥20%. Groups were compared statistically according to NLR. Results: Of the patients, 71 (79.8%) were male. The patients’ mean ages and neutrophil and lymphocyte counts were 67.36±8.64 years, 6.89±3.02 K/µL, and 3.08±2.18 K/µL, respectively. NLRs of groups 1 and 2 were 2.80±2.25 and 4.59±2.97, respectively. The relationship between group 1 and 2 tumors was significant (p=0.008). NLR values were 3.82±2.49 and 5.20±3.25 in groups 2A and 2B, respectively. However, no significant relationship was found between these values (p=0.235). Conclusion: Although no positive correlation was found between NLR and lymph node density, we think that this inflammation marker is an invaluable parameter to predict lymph node metastasis
Citation - WoS: 1
Renoprotective effect of diacerein in rats with partial unilateral ureteral obstruction model
(Walter de Gruyter, 2024) Katar, Muzaffer; Gevrek, Fikret; Fırat, Fatih; Kölükçü, Engin; Unsal, Velıd
Objectives: We aimed to analyze the effects of diacerein in a rat model of partial unilateral ureteral obstruction (PUUO). Methods: We randomly divided 24 female rats into three groups. Control group, PUUO group and PUUO + diacerein group. The PUUO group was subjected to the PUUO model for seven days. The PUUO + diacerein group received oral diacerein (80 mg/kg) for seven days. Spectrophotometric methods were employed to measure oxidative stress parameters, including malondialdehyde (MDA), protein carbonyl (PC) and antioxidant enzyme levels, including glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD), while indicators of renal function, such as kidney injury molecule-1 (KIM-1) and neutrophil gelatinous- associated lipocalin (NGAL), along with inflammatory parameters interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6), were assessed using the ELISA method. Inflammatory parameters were measured in blood samples, and other parameters were analyzed in kidney tissue. Hematoxylin-eosin method examinations were used for histological analyses. Results: IL-1beta, TNF-alpha, and IL-6 levels were found to be significantly decreased in the PUUO + diacerein group compared to the PUUO group (p=0.006, p=0.002 and p=0.001, respectively). In the PUUO + diacerein group, GSH-Px and SOD activities increased compared with the PUUO group (p=0.031 and p=0.037, respectively). We also observed a significant improvement in renal function parameters, such as KIM-1 and NGAL levels in the PUUO + diacerein group compared to PUUO (p=0.002 and p=0.012, respectively). The PC and MDA levels were highest in the PUUO group (p<0.001). Similarly, the histopathologic tissue damage was the most prominent PUUO group (p<0.001). Conclusions: Our study found that diacerein is a highly effective pharmacologic agent in alleviating oxidative dam- age in PUUO model rats.
A Research on the Determination of Aflatoxin M1 Levels in Milk and Dairy Products for Sale in Diyarbakır by ELISA
(Erzincan University Journal of Science and Technology, 2019) Yeşil, Ömer Faruk; Hatipoğlu, Abdulkerim; Yıldız, Abdunnasır; Vural, Aydın; Erkan, Mehmet Emin
In this study, the contamination levels of Aflatoxin M1 of 248 sterilized milk, Beyaz, Cecil, Cokelek, Cream, Lor, Van Otlu (herbed), Van Otlu Lavash Cheese and butter samples randomly obtained from selected market and supermarkets from Diyarbakır province center were determined by ELISA method. The average AFM1 contamination value of the cheese samples investigated was 138.65 ng/kg; while the minimum and maximum values were 50 and 595.31 ng/kg, respectively. On the other hand, the average AFM1 value of 13 sterilized milk samples was 52.59 ng/kg; minimum and maximum values were determined as 25.97 and 80.0 ng/kg, respectively. In addition, the average AFM1 value of 18 butter samples was 97.54 ng/kg; the minimum and maximum values were calculated as 50 and 308.13 ng/kg. In the study, the level of AFM1 contamination in the 26 samples of cheese samples were exceeded the limits set by the European Commission for AFM1. In terms of Turkish Food Codex, only 2 cheese samples exceeded the AFM1 tolerance limit. According to the study, the level of AFM1 contamination in the 7 milk samples and 15 butter samples exceeded both the European Commission and Turkish Food Codex AFM1 tolerance limit.

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