TR-Dizin İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/1836

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Allelic variations of glutenin subunits and their association with quality traits in bread wheat genotypes
(2017) Aktaş, Hüsnü; Baloch, Faheem Shehzad
The present study was conducted to evaluate the genotype × environment interaction of the yield and quality traits for five bread wheat varieties commonly grown in the Southeastern Anatolia Region of Turkey and 20 advanced lines developed within the framework of the International Winter Wheat Improvement Project. We also determined the allelic pattern of the Glu-1 and Glu-3 loci of these genotypes and examined whether these loci had an effect on the quality traits. There was a significant variation among the genotypes and environments in terms of grain yield, protein content, sedimentation volume (SV), and the extensograph dough energy value (EDEV). The results of the study indicated that genotypic effect was more influential on SV and EDEV than environmental effect; thus, both traits could be used in breeding programs to develop elite cultivars with better quality. Twelve different high-molecularweight (HMW) glutenin alleles were identified at the Glu-A1, Glu-B1, and Glu-D1 loci, resulting in 14 allelic combinations, and 17 different alleles were observed in 19 combinations for low-molecular-weight (LMW) subunits. Furthermore, among all the genotypes, 13 + 16 and 13 + 19 alleles at Glu-B1 and 5 + 12 at Glu-D1 were observed to have the lowest frequency. Our study indicated that the combinations of HMW glutenin alleles with 2* at Glu-A1, 17 + 18 and 13 + 16 at Glu-B1, and 5 + 10 at Glu-D1, as well as the combinations of LMW alleles with subunits c and d at Glu-A3; subunits d, b, c, and g at Glu-B3; and subunits a and b at Glu-D3 had positive effects on the quality traits.
Characterization and Bioremediation Potential of Heavy-Metal Resistant Bacteria Isolated From Agricultural Soil
(Tubitak Scientific & Technological Research Council Turkey, 2024) Dahnoun, Kheira; Djadouni, Fatima; Essghaier, Badiaa; Naccache, Chahnez; Zitouna, Nadia; Zehdi-Azouzi, Salwa; Bourguiba, Hedia
Heavy metal pollution is a major environmental issue that has a negative impact on soil quality and food security. As result, heavy metal removal or remediation from hazardous sites has become mandatory. Bioremediation based on microorganisms is promising method to remediate heavy metal-contaminated areas due to its ecofriendly, cost-effective, and highly efficient characteristics. This study aimed to isolate, identify, and characterize rhizospheric bacteria able to resist, reduce, and detoxify heavy metals [chromium (Cr), nickel (Ni), and aluminum (Al)] from agricultural soil. Two isolates were chosen due to their high level of heavy metal resistance and could serve as potential in situ remediation agents at the site of isolation. On the basis of morphological, cultural, biochemical, and molecular characterization, these two isolates were identified as Pseudomonas aeruginosa (S1) and Bacillus cereus (S2). The results revealed a minimum inhibitory concentrations (MICs) of the three heavy metals studied, ranging from 1000 to 1400 mu g/mL for the two bacterial isolates. Atomic absorption spectroscopy analysis was used to evaluate the degrading potential. B. cereus was able to reduce Cr and Al more than P. aeruginosa (42% and 67.78% vs. 38.44% and 58.85, respectively). On the other hand, P. aeruginosa showed a higher capacity to degrade Ni than B. cereus (62.33% and 50.76%, respectively). The findings of the analysis revealed information regarding the use of these heavy metal-resistant bacterial isolates as potential bioremediation agents in contaminated environments. Microbial bioremediation offers sustainable alternatives to the traditional physical or chemical remediation technologies of agricultural land.
The Involvement of the Serotonergic System in Ketamine and Fluoxetine Combination-Induced Cognitive Impairments in Mice
(Ataturk Univ, 2024) Uyar, Emre; Erdinc, Meral; Kelle, Lker; Erdinc, Levent; Seker, Ugur; Nergiz, Yusuf
Background: Gluta mater gic N-methyl-D-aspartate (NMDA) receptors play vital roles in memory formation. Changes in the activity of these receptors influence memory processes. Ketamine is a noncompetitive NMDA receptor antagonist drug with promising mood-altering and pain-reducing effects ff ects in low doses. These effects ff ects are believed to be related to altered serotonergic transmission. Methods: The present study investigated the involvement of the serotonergic system in low-dose ketamine administrations' effects ff ects on memory acquisition, consolidation, and retrieval processes. Sixty-four male BALB/c mice were used in this experiment and separated into 8t groups. Mice were treated subchronically with a selective serotonin reuptake inhibitor, fluoxetine, and a serotonin depletion agent, p-chlorophenylalanine (pCPA). A serotonin antagonist, methiothepin, and ketamine were acutely administered 60 minutes before or after the behavioral tests. A passive avoidance (PA) test measured emotional memory acquisition, consolidation, and retrieval processes. Hippocampi malondialdehyde (MDA) levels were analyzed, and histopathological examinations were performed. Results: Ketamine alone did not significantly affect ff ect memory encoding processes in the PA test, while the ketamine-fluoxetine combination disrupted memory consolidation. Fluoxetine negatively affected ff ected the memory acquisition process, which was normalized during the consolidation and retrieval trials. Drug applications did not significantly alter hippocampal MDA levels. In all ketamine-applied groups, histopathologic alterations were evident. Conclusion: Low-dose ketamine administration induces neurodegeneration, and it also impairs memory functions when combined with fluoxetine, indicating increased serotonergic transmission may be involved in the memory-impairing and neurotoxic effects ff ects of ketamine.

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