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1-(2-Hydroxyethyl)-2-imidazolidinone as corrosion inhibitor of mild steel in 0.5 M HCl solution: thermodynamic, electrochemical and theoretical studies
(Journal of Adhesion Science and Technology, 2019) Keleşoğlu, Ayşen; Yıldız, Reşit; Dehri, İlyas; Yıldız, Reşit
The inhibition effect of 1-(2-Hydroxyethyl)-2-imidazolidinone (2-HEI) on mild steel (MS) corrosion in 0.5M HCl solution was investigated at different inhibitor concentration and temperature by electrochemical experiments, such as linear polarization resistance (LPR), electrochemical impedance spectroscopy (EIS), potentiodynamic polarization and quantum chemical calculations. The inhibitor adsorption process on mild steel in 0.5M HCl system was studied at different temperatures (20 C–50 C). Furthermore, the surface morphology of MS was also investigated with SEM in the absence and the presence of inhibitor. The adsorption of 1-(2-Hydroxyethyl)-2-imidazolidinone on MS surface is an exothermic process and this process obeys the Langmuir adsorption isotherm. The Quantum chemical findings are good agreed with the empirical data.
Alpha-lipoic acid may ameliorate testicular damage by targeting dox-induced altered antioxidant parameters, mitofusin-2 and apoptotic gene expression
(Andrologia, 2021) Güzel, Elif Erdem; Kaya Tektemur, Nalan; Tektemur, Ahmet
In the study, the ameliorating effects of alfa lipoic acid (ALA) against doxorubicin-induced testicular apoptosis, oxidative stress and disrupted mitochondrial fusion were investigated in male rats. Rats were divided into four groups as control, doxorubicin (DOX), DOX + ALA and ALA. A single dose of 15 mg/kg DOX was administered i.p to the DOX and DOX + ALA groups. 50 mg/kg ALA was given to the DOX + ALA and ALA groups by oral gavage every other day. After 28 days, rat testes and serum samples were collected and analysed. Administration of DOX alone caused a decrease in body and relative testicular weights, seminiferous tubule diameter and germinal epithelium thickness, Johnsen's score and serum testosterone levels. DOX treatment led to severe testicular damage such as tubular degeneration, and atrophic tubules. Also, the activities of superoxide dismutase and glutathione peroxidase were reduced, while the level of malondialdehyde was increased in the testis. The mRNA levels of apoptotic-related genes (CASP3, TP53, BAX, BCL2) and apoptotic index were increased, while mitofusin-2 decreased. DOX caused an increase in CASP3 and a decrease in mitofusin-2 immunoreactivities. Treatment with ALA markedly improved all of DOX-induced biochemical, histochemical and molecular alterations in rat testis. Consequently, ALA has a therapeutic role in ameliorating DOX-induced testicular damage in rats.
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
The antioxidant and anti-apoptotic potential of Pleurotus eryngii extract and its chitosan-loaded nanoparticles against doxorubicin-induced testicular toxicity in male rats
(Wiley, 2021) Erdem Güzel, Elif; Acay, Hilal; Yıldırım, Ayfer; Acay, Hilal; Yıldırım, Ayfer
This study was conducted to evaluate the protective role of Pleurotus eryngii extract (PE) and Pleurotus eryngii extract-loaded chitosan nanoparticles (PE-CSNP) against doxorubicin (DOX)-induced testicular toxicity in rats. Male rats were divided into six groups: control (DMSO/ethanol), PE (200 mg/kg PE), PE-CSNP (30 mg/kg PECSNP), DOX (10 mg/kg DOX, a single dose, i.p), DOX+PE (10 mg/kg DOX+200 mg/ kg PE) and DOX+PE-CSNP (10 mg/kg DOX+30 mg/kg PE-CSNP). PE and PE-CSNP were administered by oral gavage every other day for 21 days. DOX-treated rats showed histopathological impairment compared with the control group. There was an increase in the apoptotic index, caspase 3 (CASP3), BCL2-associated X apoptosis regulator (BAX), dynamin-related protein 1 (DRP1) expression and total oxidative status (TOS) in the DOX group, while mitofusin-2 (MFN2), total antioxidative status (TAS) and serum testosterone levels of the DOX group reduced when compared with the other groups. PE and PE-CSNP treatments provided significant protection against DOX-induced oxidative stress by reducing TOS levels and increasing TAS levels. CASP3, BAX, apoptotic index and DRP1-MFN2 expressions were restored by PE and PE-CSNP. However, the PE-CSNP showed higher antioxidant and anti-apoptotic efficacy compared with PE. Thus, our results provide evidence that CSNP and PE could synergistically have a potent antioxidant and anti-apoptotic therapy against DOX-induced testicular damage in male rats.
Biochemical components, enzyme inhibitory, antioxidant and antimicrobial activities in endemic plant Scilla mesopotamica speta
(Wiley, 2021) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Akmeşe, Şükrü; Akmeşe, Şükrü
In this study, in vitro antioxidant, antimicrobial, anticholinesterase and phenolic profile of different solvent extracts of Scilla mesopotamica speta were determined in detail. In vitro antioxidant activities and total phenolic and flavonoid contents of plant extracts obtained with different solvents were tested in terms of 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities. The highest total phenolic and total flavonoid contents were determined in the ethyl acetate extract (62.24 mu g GAE/mg) and chloroform extract (87.72 mu g QE/mg) respectively. The highest DPPH radical scavenging activity was detected in ethyl acetate extracts. Antimicrobial and antifungal activities were investigated by MIC method. The inhibitory activities of the extracts on the acetyl cholinesterase enzyme were investigated. Liquid chromatography (LC) tandem mass spectrometry LC-MS/MS was used to determine the phenolic component content of extracts. Thirty-one different components were identified in the analyses and their amounts were measured. Practical applications Scilla mesopotamica speta is an endemic and medicinal plant. It was determined that the extracts of this plant had a very rich content in terms of phenolic compounds, especially caffeic and ferulic acids. However, this plant was remarkable for its antioxidant, anticholinesterase, and antimicrobial activities. Considering the strong antioxidant, antimicrobial, and enzyme inhibition activities of the Scilla mesopotamica speta it can be suggested as a source of anticancer, antimicrobial, and antiviral drugs.
Carbamazepine-induced renal toxicity may be associated with oxidative stress and apoptosis in male rat
(Taylor & Francis Online, 2021) Erdem Güzel, Elif; Kaya Tektemur, Nalan; Tektemur, Ahmet; Etem Önalan, Ebru
Carbamazepine (CBZ) is the antiepileptic drug used in epilepsy and some psychiatric disorders. Besides its widely used, many adverse effects have been reported including hematotoxicity, hepatotoxicity, endocrine disorders, and testicular damages due to oxidative stress. However, the role of CBZ on renal toxicity is not fully known. In this study, we attempted to explain the connected mechanisms by focusing on the metabolism of CBZ-induced renal toxicity in rats. Twenty male Wistar-Albino rats were randomized into 2 groups (n = 10); control (1 mL/day distilled water, orally) and CBZ (25 mg/kg/day CBZ, orally) groups. After 60 days, TAS (total oxidant status) and TOS (total oxidant status) levels, histopathological features, some genes involved in apoptosis, 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, and apoptotic cells were assessed of kidney tissue. The oxidative stress index (OSI) was measured from TAS and TOS levels. TOS levels and OSI significantly increased, while TAS levels decreased in the CBZ group relative to the control group. Histopathological observations, Caspase-3 (Casp3), Poly [ADP-ribose] polymerase-1 (PARP-1), 8-OHdG immunoreactivities, and apoptotic cells markedly raised in the CBZ group compared with the control group. Also, mRNA expression of Cytochrome c (Cytc) and CASP3 significantly increased in the CBZ group compared to the control group. In conclusion, long-term use of CBZ may promote renal damage in rats by inducing oxidative stress and apoptosis.
Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring
(Taylor & Francis Online, 2020) Erdem Güzel, Elif; Nalan Kaya, Ahmet Tektemur, Nazife Ulker, Ahmet Yardimci, Ramazan Fazil Akkoc, Sinan Canpolat & Ibrahim Enver
Prenatal tobacco-smoke exposure negatively affects the reproductive functions of female offspring and oxidative stress plays a major role at this point. Alpha-lipoic acid (ALA), well known as a biological antioxidant, has been used as a nutritional supplement and as a therapeutic agent in the treatment of certain complications during pregnancy. We aimed to investigate the effects of maternal tobacco-smoke exposure and/or ALA administration on puberty onset, sexual behavior, gonadotrophin levels, apoptosis-related genes, apoptotic cell numbers and oxidative stress markers in the adult female rat offspring. Sprague-Dawley rats were divided into four groups; control, tobacco smoke (TS), TS+ALA and ALA groups. Animals were exposed to TS and/or ALA for 8 weeks before pregnancy and throughout pregnancy. All treatments ended with birth and later newborn female rats were selected for each experimental group. The experiment ended at postnatal day 74-77. Maternal tobacco smoke advanced the onset of puberty in the female offspring of the TS group (p < 0.05). In all treatment groups; the mean number of anogenital investigations and lordosis quality scores showed a decline, serum luteinizing hormone levels significantly increased (p < 0.05) and several histopathological changes in ovaries were observed compared to the control group. In addition, an increase in apoptotic marker levels and apoptotic cell numbers was detected in the ovaries of all treatment groups. Decreased TAS and increased TOS levels were detected in all treatment groups compared to control. These findings suggested that maternal tobacco smoke and/or ALA administration may be leading to the impaired reproductive health of female offspring. Abbreviations: ALA: alpha-lipoic acid; LH: luteinizing hormone; FSH: follicle-stimulating hormone; TAS: total antioxidant status; TOS: total oxidant status; Apaf1: apoptotic protease-activating factor 1; Casp3: caspase 3; Casp9: caspase 9; CF: cyst follicles; 4-HNE: 4-Hidroxynonenal; 8-OHdG: 8-hydroxydeoxyguanosine; TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine-biotin nick end labeling; ROS: reactive oxygen species; GnRHR: gonadotropin-releasing hormone receptor; HPG: hypothalamic-pituitary-gonadal; AMPK: AMP-activated protein kinase; ELISA: enzyme-linked immunosorbent assay; cDNA: complementary DNA; qPCR: quantitative real-time PCR; FC: follicular cysts; PF: primary follicle; SF: secondary follicle; GF: graafian follicle; CL: corpus luteum; DF: degenerated follicle; AF: atretic follicle.
Eating Behavior Changes of People With Obesity During the Covid-19 Pandemic
(Dove Medical Press Ltd, 2021) Deger, Vasfiye Bayram
Objective: The precautions taken during the pandemic period may cause stress-related eating behavior disorders. It was aimed to test this hypothesis, and the study was carried out to examine pandemic measures the effect of on the nutritional, depression and stress conditions of people with obesity. Methods: The individuals who participated in the study were people with obesity who received follow-up dietary therapy in a private hospital. Three separate scales were applied to the individuals, which measured the desire to overeating request, depression status and stress-fighting status. Results: This study was conducted on 368 individuals. Women had lower values of BMI (28.57 +/- 3.89 kg/cm(2)) than men ( 30.64 +/- 2.87 kg/cm(2)). When the scores of the excessive eating request scale mean scores before and during the pandemic were examined, it was seen that the scores of the individuals increased during the pandemic. In the multivariate regression model, it was seen that the increase of stress and BMI increased the FCQ score (p<0.001). Multiple regression models were created by taking into account the criteria that caused the score increase. Each variable can predict the FCQ score separately. The predictor significance order of variants on FCQ score beta values is as follows: the Patient Health Questionnaire-9 (PHQ-9) (beta=0.774), before pandemic FCQ (beta = 0.601), the Perceived Stress Scale ( PSS) (beta=-0.268), before pandemic BMIa (beta=-0.223), during pandemic BMIb (beta=0.073), and age (beta=-0.013). Conclusion: COVID-19 pandemic, making applications such as quarantine in pandemic processes has successful results in being able to combat its. However, undesirable conditions such as stress can have serious negative consequences on other health measurements. It was observed in the results of this study that excessive eating food desire developed in people with obesity.
The effect of acupressure on postoperative pain of lumbar disc hernia: A quasi-experimental study
(ELSEVIER SCI LTD, 2018) Tanrıverdi, Seher; Sarıtaş, Serdar
Lumbar disc herniation (LDH) is a disease characterized by lumbar and leg pain that occurs as a result of compression of the lumbar spinal root by the degenerated disc. The results of the study showed that 80% of humans experienced lumbar pain in any period of their lives [[1], [2], [3]]. Comprising an important part of lumbar pain; lumbar disc herniation (LDH) has an prevalence of approximately 15–56% per year [4,5]. One of the treatment methods preferred for patients diagnosed with lumbar disc herniation is surgery. Patients, who undergo surgery, experience intense pain in the early postoperative period [6]. It is known that medications are commonly used in pain control. Analgesic treatment used for pain control is the most preferred treatment method for pain relief as it has a rapid action and is administered easily [7,8]. Damages in both individual and national economy due to preferring carelessly and frequently analgesics affect negatively some physiological functionings and have negative effects such as development of addiction with the increase of dosages every time especially in cases where narcotics are preferred [9]. Thus, it is indicated that the administration of non-pharmaceutical methods by nurses to relieve the postoperative pain in patients in addition to medications increases the action of medications [8]. It is anticipated that there are numerous non-pharmaceutical methods which are efficient in pain management, such as distraction, relaxation, therapeutic touch, transcutaneous nerve stimulation (TENS), informing, hypnotherapy, acupuncture, massage, aromatherapy, music and meditation, yoga, attention-grabbing, imagination, cognitive-behavioral techniques. But, it is known that nurses do not use these methods adequately to relieve pain [10]. It may be asserted that non-pharmaceutical treatments are among independent nursing roles of professional nurses who have theoretical knowledge and problem solving ability. Nurses are required to take a role in non-pharmaceutical interventions in order to meet the health needs of individuals, families and society [11]. One of the non-pharmaceutical treatment methods used in pain relief is acupressure. Applied with touch pressuring on the acupuncture points using hands, fingers, thumbs or small beads according to the traditional Chinese medicine; acupressure is a non-pharmaceutical pain relief example that balances energy in the body [[12], [13], [14]]. Advantages of acupressure include low cost, learning convenience and non-invasive characteristics [14]. Besides, randomized clinical studies conducted to compare physiotherapy and acupressure in decreasing lumbar pain (LBP) have reveal that acupressure might be effective [[15], [16], [17]]. The use of acupressure in nursing practices by taking all its positive effects into consideration will contribute to the establishment of qualified nursing care standards.
The Effect of Progressive Muscle Relaxation on Abdominal Pain and Distension in Colonoscopy Patients
(ELSEVIER, 2023) Tanrıverdi, Seher
Purpose:This study was conducted to examine the effect of progressive muscle relaxation on abdominal pain and distension in colonoscopy patients. Design:A randomized controlled experimental design. Methods:The experimental group was informed about progressive muscle relaxation(PMR) and anaudio recording was provided. After the colonoscopy,abdominal pain(VisualAnalogueScale(VAS)pain) and distension (VAS distension) scores were assessed, then PMR was applied for 30 minutes and pain scores were determined again.VAS pain and VAS distension scores of the control group were determined after colonoscopy and 30 minutes later.VAS abdominal pain and VAS distension scores were evaluated at the 2nd,4th, 8th,12th,16th,and 24th hour after the procedure on all patients.The day after thec olonoscopy, post-test data were collected. Findings:The pretest VAS pain and VAS distension mean scores were similar in thei ntervention and control groups(P>.05). The mean VAS pain and VAS distension scores decreased significantly in thei ntervention group post procedure(P<.05). Conclusions:PMR was determined to be effective in reducing abdominal pain and distension after colonoscopy.
Effect of seasonal variation on lipid and fatty acid profile in muscle tissue of male and female Silurus triostegus
(SPRINGER INDIA, 2016) Kacar, Semra; Bashan, Mehmet; Oymak, Seyit Ahmet
Fatty acid (FA) compositions of total lipid, phospholipid (PL) and triacylglycerol (TAG) fractions have been determined in muscle tissues of Silurus triostegus. The distributions of saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) proportions were found to be different among total lipid, PL and TAG fractions from lipids in muscle tissues of the male and female S. triostegus in all seasons. Triacylglycerol contained a lower proportion of PUFA and a higher proportion of MUFA and SFA than PL while PL contained higher proportion of PUFA than proportion of MUFA and SFA compared to TAG. Triacylglycerol and PL fatty acid compositions in muscle tissues of the male and female fish species were found different. The most abundant fatty acids in the investigated seasons were palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1 n-9), palmitoleic acid (C16:1 n-7) and docosahexaenoic acid (C22:6 n-3). It was shown that the total lipid content and FA compositions in muscle tissues of the male and female S. triostegus were influenced by reproduction period and season.
The effects of oxytocin on penile tissues in experimental priapism model in rats
(SPRINGER, 2019) Kolukçu, Engin; Kılıç, Şahin; Parlaktaş, Bekir Süha; Erdemir, Fikret; Ünsal, Velid; Atılgan, Doğan; Uluocak, Nihat
PurposeThis study aimed to demonstrate the effects of oxytocin on penile tissues in ischemia-reperfusion injury developed after priapism.MethodsForty Wistar Albino strain male rats were divided into four groups. The control group (n=10) was not intervened. In Group 2, a rat model of priapism was constructed and maintained for 1 h. In Group 3, reperfusion was ensured for 30min following priapism. Rats in Group 4 rats were given oxytocin 30min before the induction of reperfusion following priapism. All rats were penectomized, and adequate amounts of blood sample were drawn. Inflammation, vasocongestion, desquamation, and edema in penile tissue were scored between 0 and 3 points (0: normal, 1: mild, 2: moderate, 3: severe) to evaluate the severity of tissue damage. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and the levels of malondialdehyde (MDA), and nitric oxide (NO) in blood samples were determined spectrophotometrically.ResultsIn histopathological examination, statistically significant positive changes were detected in vasocongestion, inflammation, desquamation, and edema scores in Group 4 than in Group 2 and Group 3 (p<0.001). Biochemical test results revealed that NO levels were significantly lower in Group 4 than in Group 3 (p<0.001). Serum GSH-Px activities in Group 4 significantly increased when compared with the other groups 2 and 3 (p=0.002, p=0.001, respectively). There was no statistical difference among the groups regarding SOD activities and MDA levels (p>0.05).ConclusionsOxytocin protected against priapism-induced ischemia-reperfusion injury developed in cavernosal tissue as observed based on histopathological and biochemical evidence. Although this is an experimental study, oxytocin can be thought as an alternative drug in the treatment of priapism.
ERUCA SATIVA MILL SEEDS OIL ALLEVIATES HYPERLIPIDEMIA AND NON-ALCOHOLIC FATTY LIVER DISEASE IN SYRIAN HAMSTER
(2022) Alhilal, M; Sulaiman, Y A M; Subuh, A M; Habra, N; Alhilal, S
The impact of oils rich in long chain monounsaturated fatty acids (LCMUFA) against hyperlipidemia and non-alcoholic fatty liver disease (NAFLD) has been inadequately described. In addition, the chemical solvents and the high temperature used in vegetable oils extraction process from seeds cause severe loss of many vital compounds. So the goal of this paper was to examine the effect of cold pressed Eruca sativa Mill seeds oil (ESSO), as a source of LCMUFA, on hyperlipidemia and NAFLD in Syrian hamster. The ESSO content of fatty acids was analyzed using chromatographic methods. Fifty two (52) healthy male golden Syrian hamsters used in this experiment were randomly divided into 4 groups (Completely Randomized Design). Negative control group, CHD group, positive control group and ESSO group. This experiment was achieved in two periods. The first period continued 4 weeks, in which hyperlipidemia and NAFLD were induced in CHD, positive control and ESSO groups through feeding on a hyperlipidemic diet. The second period also lasted 4 weeks, in which ESSO was orally gavaged at 2 g/kg of the body weight daily to animals of ESSO group. The levels of total cholesterol (TC), triglycerides, HDL-C and glucose and the activities of ALT, AST, ALP, LDH and CK were analysed in the serum. One way analysis of variance (ANOVA) followed by Duncan's multiple range test was used for statistical analysis. The consumption of hyperlipidemic diet for 4 weeks caused a significant raise (P<0.05) of triglycerides, glucose, ALT, AST, LDH, CK and a significant reduction (P<0.05) of the HDL-C/TC ratio, at the same time created lipid accumulation in liver cells in CHD, positive control and ESSO groups in comparison with negative control group at the end of the first period. These negative influences were alleviated in ESSO group by administration of ESSO at the end of second period. In conclusion, The examined cold pressed ESSO has effective hypolipidemic and hepatoprotective effects in Syrian hamsters with hyperlipidemia and NAFLD.
ERUCA SATIVA MILL SEEDS OIL ALLEVIATES HYPERLIPIDEMIA AND NONALCOHOLIC FATTY LIVER DISEASE IN SYRIAN HAMSTER
(Journal of Animal & Plant Sciences, 2022) Alhilal, M.; Sulaiman, Y.A.M.; Subuh, A.M.; Habra, N.; Alhilal, S.
The impact of oils rich in long chain monounsaturated fatty acids (LCMUFA) against hyperlipidemia and non-alcoholic fatty liver disease (NAFLD) has been inadequately described. In addition, the chemical solvents and the high temperature used in vegetable oils extraction process from seeds cause severe loss of many vital compounds. So the goal of this paper was to examine the effect of cold pressed Eruca sativa Mill seeds oil (ESSO), as a source of LCMUFA, on hyperlipidemia and NAFLD in Syrian hamster. The ESSO content of fatty acids was analyzed using chromatographic methods. Fifty two (52) healthy male golden Syrian hamsters used in this experiment were randomly divided into 4 groups (Completely Randomized Design). Negative control group, CHD group, positive control group and ESSO group. This experiment was achieved in two periods. The first period continued 4 weeks, in which hyperlipidemia and NAFLD were induced in CHD, positive control and ESSO groups through feeding on a hyperlipidemic diet. The second period also lasted 4 weeks, in which ESSO was orally gavaged at 2 g/kg of the body weight daily to animals of ESSO group. The levels of total cholesterol (TC), triglycerides, HDL-C and glucose and the activities of ALT, AST, ALP, LDH and CK were analysed in the serum. One way analysis of variance (ANOVA) followed by Duncan's multiple range test was used for statistical analysis. The consumption of hyperlipidemic diet for 4 weeks caused a significant raise (P<0.05) of triglycerides, glucose, ALT, AST, LDH, CK and a significant reduction (P<0.05) of the HDL-C/TC ratio, at the same time created lipid accumulation in liver cells in CHD, positive control and ESSO groups in comparison with negative control group at the end of the first period. These negative influences were alleviated in ESSO group by administration of ESSO at the end of second period. In conclusion, The examined cold pressed ESSO has effective hypolipidemic and hepatoprotective effects in Syrian hamsters with hyperlipidemia and NAFLD.
Evaluation and characterization of Pleurotus eryngii extract-loaded chitosan nanoparticles as antimicrobial agents against some human pathogens
(Taylor & Francis Online, 2020) Acay, Hilal; Yıldırım, Ayfer; Erdem Güzel, Elif; Baran, Mehmet Fırat; Baran, Mehmet Fırat
With the increase of antibiotic resistance, which is present at a worrying rate, research on the use of newly developed nanoparticles as an antimicrobial agent with green biotechnology has intensified. The study aimed to investigate the antimicrobial effects of chitosan nanoparticles (CSNP) synthesized using Pleurotus eryngii extract (PE). Characterization of P. eryngii-loaded chitosan nanoparticles (PE-CSNPs) was performed with Fourier transform infrared spectrophotometer, X-ray diffraction, Field-emission scanning electron microscopy, Brunauer-Emmett-Teller, Differential scanning calorimetry, and zeta potential techniques. The FE-SEM images showed that the surface morphology of nanoparticles is similar to CS, but has more porosity network and smaller dimensions structure. The average particle size of spherical PE-CSNPs was obtained as 330.1 nm. The specific surface area and average pore diameter of the synthesized nanoparticles were found as 3.99 m2g-1 and 2.25 nm, respectively. X-ray diffraction determines the presence of an amorphous peak at 2θ = 21.2° results from CS and PE. PE-CSNPs synthesized using P. eryngii extract showed strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans as 0.0156, 0.0625, 0.0625 and 0.0312 mg ml-1, respectively. Thus, it was determined that chitosan nanoparticles formed by the green synthesis of P. eryngii extract showed strong anti-microbial properties.
Experimental and theoretical study on hydrogen production by using Ag nanoparticle‐decorated graphite/Ni cathode
(2021) Yıldız, Reşit; Doğru, Mert, Başak; KARAZEHİR, TOLGA; GÜRDAL DURĞUN, YELİZ; Toprak Döşlü, Serap; Yıldız, Reşit; Toprak Döşlü, Serap
In this study, graphite (G) electrode was coated with nickel and decorated with silver nanoparticles (G/Ni/Ag) with the help of galvanostatic method, and electrodes were used as a cathode in alkaline water electrolysis system. The characterization was achieved using X-ray diffraction and field emission scanning electron microscopy. Hydrogen evolution performance of electrodes was investigated via cyclic voltammetry, chronoamperometry, cathodic polarization curves, and electrochemical impedance measurements. Electrochemical results showed that hydrogen production efficiency significantly increased and charge transfer resistance decreased via G/Ni/Ag. The electrochemical water splitting performance of G/Ni/Ag, was established in a joint experimental and computational effort. Water and proton adsorption on Ag-decorated Ni surface were investigated using density functional theory. Electronic structure calculations identified the role of Ag adatom and Ni surface on water and proton adsorptions. From the computational studies, O in water was more reliable to adsorb at the bridge position of the Ag and Ni atoms, leading improved orbital overlap between H and Ni atoms and maximized chemical and physical interactions between the H2O molecules. Therefore, the Ag-decorated Ni(111) surface provides preferable adsorption site for the O atom in water and direct interactions between water Hs and available surface Ni atoms promote water dissociation.
Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101
(Wiley, 2021) Aktepe, Necmettin; Yukselten, Yunus
The aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.
Investigation of the Necessity of Aspiration During the Intramuscular Injection Administered in the Ventrogluteal Site and Its Effect on Pain: A Randomized Controlled Trial
(Sage Journals, 2022) Baran, Leyla; Güneş, Ülkü; Dönmez, Hilal
This experimental study, which was conducted to examine the necessity of aspiration procedure and its effect on pain in intramuscular (IM) injections made into the ventrogluteal site (VGS), is randomized controlled and double-blind. The patients in the study group (n = 834) were assigned to the IM group with the aspiration period of 5 to 10 seconds (Implementation Group A-IGA), the aspiration period of 1 to 2 seconds (Control Group-CG), and no aspiration (Implementation Group B-IGB) according to stratified block randomization list. Patients’ pain levels were evaluated with the Visual Analog Scale (VAS). No bleeding was observed when aspiration periods of 1 to 2 and 5 to 10 seconds were followed during the injections administered to the VGS. The difference between the pain medians of patients in IGB and the CG were not significant (p =.521). It can be said that there is no need to apply aspiration in IM applied into the VGS if the correct site is determined
Investigation of the Necessity of Aspiration During the Intramuscular Injection Administered in the Ventrogluteal Site and Its Effect on Pain: A Randomized Controlled Trial
(2023) Baran, Leyla; Güneş, Ülkü; Dönmez, Hilal
Abstract This experimental study, which was conducted to examine the necessity of aspiration procedure and its effect on pain in intramuscular (IM) injections made into the ventrogluteal site (VGS), is randomized controlled and double-blind. The patients in the study group (n = 834) were assigned to the IM group with the aspiration period of 5 to 10 seconds (Implementation Group A-IGA), the aspiration period of 1 to 2 seconds (Control Group-CG), and no aspiration (Implementation Group B-IGB) according to stratified block randomization list. Patients’ pain levels were evaluated with the Visual Analog Scale (VAS). No bleeding was observed when aspiration periods of 1 to 2 and 5 to 10 seconds were followed during the injections administered to the VGS. The difference between the pain medians of patients in IGB and the CG were not significant (p = .521). It can be said that there is no need to apply aspiration in IM applied into the VGS if the correct site is determined.
Milrinone ameliorates ischaemia-reperfusion injury in experimental testicular torsion/detorsion rat model
(Andrologia, 2021) Unsal, Velid; Kölükçü, Engin; Atılgan, Doğan; Uluocak, Nihat; Deresoy, Faik Alev; Katar, Muzaffer
This experimental study aims to evaluate the efficacy of milrinone against ischaemia-reperfusion injury due to testicular torsion/detorsion. Group 1 was defined as the control group. Testicular torsion/detorsion model was performed in Group 2. Group 3 had similar procedures to the rats in Group 2. In addition, 0.5 mg/kg of milrinone was administered intraperitoneally immediately after testicular torsion in Group 3. Histopathological examinations indicated a dramatic improvement in terms of inflammation, haemorrhage, oedema, congestion, Cosentino and Johnson scores in Group 3 compared to Group 2 (p =.037, p =.045, p =.018, p =.040, p =.033 and p =.03 respectively). Blood biochemical analyses, superoxide dismutase (SOD), glutathione peroxidase (GSH-px) activity and total antioxidant status (TAS) levels increased significantly in Group 3 compared to Group 2 (p =.001, p =.024 and p <.001). Malondialdehyde (MDA), protein carbonyl (PC), interleukin 1beta (IL-1beta), tumour necrosis factor-alpha (TNF-alpha) and total oxidant status (TOS) levels decreased in Group 3 compared to Group 2 (p =.001, p =.018, p <.001, p =.036 and p =.002 respectively). Tissue biochemical analyses determined an increase in SOD and GSH-px activity in Group 3 compared to Group 2, while PC and MDA levels were reduced (p =.001, p <.001, p =.038 and p <.001 respectively). Milrinone attenuates ischaemia-reperfusion injury that causes highly harmful effects due to testicular torsion/detorsion.

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