Alhılal, Mohammad
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Alhilal, Mohammad
Alhilal, M
Alhilal, M.
ALHILAL, Mohammad
Alhilal, M
Alhilal, M.
ALHILAL, Mohammad
Job Title
Doktor Öğretim Üyesi
Email Address
mohammadalhilal@artuklu.edu.tr
Main Affiliation
Department of Nursing / Hemşirelik Bölümü
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Website
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Sustainable Development Goals
3
GOOD HEALTH AND WELL-BEING
5
Research Products
6
CLEAN WATER AND SANITATION
0
Research Products
9
INDUSTRY, INNOVATION AND INFRASTRUCTURE
0
Research Products
16
PEACE, JUSTICE AND STRONG INSTITUTIONS
0
Research Products
1
NO POVERTY
0
Research Products
5
GENDER EQUALITY
0
Research Products
10
REDUCED INEQUALITIES
0
Research Products
15
LIFE ON LAND
0
Research Products
7
AFFORDABLE AND CLEAN ENERGY
0
Research Products
12
RESPONSIBLE CONSUMPTION AND PRODUCTION
0
Research Products
8
DECENT WORK AND ECONOMIC GROWTH
0
Research Products
14
LIFE BELOW WATER
1
Research Products
17
PARTNERSHIPS FOR THE GOALS
0
Research Products
11
SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
4
QUALITY EDUCATION
0
Research Products
2
ZERO HUNGER
0
Research Products
13
CLIMATE ACTION
0
Research Products
Documents
16
Citations
115
h-index
7
Documents
14
Citations
111
Scholarly Output
17
Articles
17
Views / Downloads
20/0
Supervised MSc Theses
0
Supervised PhD Theses
0
WoS Citation Count
106
Scopus Citation Count
103
WoS h-index
6
Scopus h-index
6
Patents
0
Projects
0
WoS Citations per Publication
6.24
Scopus Citations per Publication
6.06
Open Access Source
14
Supervised Theses
0
| Journal | Count |
|---|---|
| Journal of Taibah University for Science | 2 |
| BMC Chemistry | 1 |
| Catalysts | 1 |
| Experimental Biomedical Research | 1 |
| Journal of Animal and Plant Sciences | 1 |
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17 results
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Now showing 1 - 10 of 17
Article Citation - WoS: 1Citation - Scopus: 1Synthesis, Spectroscopic Characterization, and Biological Evaluation of a Novel Acyclic Heterocyclic Compound: Anticancer, Antioxidant, Antifungal, and Molecular Docking Studies(MDPI, 2025) Alhilal, Mohammad; Alhilal, Suzan; Sabancilar, Ilhan; Gomha, Sobhi M.; Elhenawy, Ahmed A.; Ouf, Salama A.Background/Objectives: This study aimed to synthesize a novel, high-molecular-weight acyclic heterocyclic compound, compound 5, via a one-pot reaction between Trichloroisocyanuric acid (TCCA) and ethanolamine, and evaluate its anticancer, antioxidant, and antifungal activities. Methods: Its complex tetrameric structure, assembled through N-N linkages, was unequivocally confirmed by a full suite of spectroscopic techniques including IR, 1H & 13C NMR, 2D-NMR, and high-resolution mass spectrometry (LC/Q-TOF/MS). The MTT assay was used to assess the anticancer activity of compound 5 against four different human cancer cell lines. Results: The findings indicate that human colon (HT29) and ovarian (OVCAR3) cancer cells were sensitive to the treatment, whereas brain (glioblastoma) (T98G) cancer cells were resistant. The most pronounced cytotoxic effect was observed in pancreatic (MiaPaCa2) cancer cells. Notably, compound 5 exhibited potent antifungal properties, achieving 100% inhibition of the pathogenic water mould Saprolegnia parasitica zoospores at 100 mu M after 10 min. Molecular docking studies corroborated the biological data, revealing a high binding affinity for key cancer and fungal targets (Thymidylate Synthase and CYP51), providing a strong mechanistic basis for its observed activities. Conclusions: These findings establish compound 5 as a promising dual-action agent with significant potential as both a targeted anticancer lead and an eco-friendly antifungal for applications in aquaculture.Article Citation - WoS: 1Citation - Scopus: 1Isocarpine as A New Peperidine Alkaloid from Adenocarpus Complicatus with Anticandidal Activity(Ingenta Connect, 2022) Alhilal, Mohammad; Alhilal, Suzan; Gomha, SobhiBackground: Adenocarpus complicatus L. is one of Adenocarpus genus, which has about 50 species, from Fabaceae (Papilionaceae) family. This plant is found in the Mediterranean basin as a southern west part of Europe and North Afric forestes. Objective: Isolation of Isocarpine From Adenocarpus Complicatus as Anticandidal Agent. Methods: A new piperidine alkaloid Isocarpine was isolated from the aerial parts of Adenocarpus complicatus L., which is gross in Syria. The structure of the new compound was determined by UV, IR, EI-Ms, 1H-NMR, 13C-NMR, DEPT-135, DQF-COSY and HMQC. Results: The anticandidal activity of isocarpine was screened against 38 Candida strains and showed remarkable inhibitory effect compared with fluconazole as an antifungal reference drug. Conclusion: Isocarpine is antifungal agent.Article Probiotics Containing Lactobacillus Are Promising Candidates for Cisplatin-Induced Cardiotoxicity(Nature Portfolio, 2025) Alhilal, Mohammad; Dabaniyasti, Eylul Turunc; Sengul, Emin; Ucar, Mahmut; Yilmaz, Ahmet; Alhilal, Suzan; Yildirim, SerkanThis study examined the effects of the mixture of probiotics Lactobacillus (Lactobacillus rhamnosus, Lactobacillus fermentum, and Lactobacillus brevis) against cardiotoxicity induced by cisplatin (CP) in rats by monitoring markers of oxidative stress, inflammation, and apoptosis in cardiac tissue. For this study, 28 male rats were divided into four groups as follows: Control, CP, Lactobacillus + CP, and Lactobacillus. Experimental cardiotoxicity was induced by CP. The activity of cardiac enzymes in serum and indicators of oxidative stress and nitric oxide (NO) in heart tissues were measured using biochemical methods. Inflammation, apoptosis, and DNA damage were also assessed in cardiac tissue by immunohistochemistry and immunofluorescence. CP administration caused a significant increases (p < 0.01) in malondialdehyde (1.53 +/- 0.21), NO (77.83 +/- 4.26), and 8-hydroxydeoxyguanosine (8-OHdG) (80.50 +/- 1.64) levels as well as marked decreases (p < 0.01) in the activity of superoxide dismutase (0.93 +/- 0.15) and the level of glutathione (1.87 +/- 0.20) in the CP group compared with those in the control group (0.88 +/- 0.19, 53.67 +/- 5.32, 21.67 +/- 0.81, 1.39 +/- 0.29, 2.64 +/- 0.28, respectively). This imbalance in the oxidant/antioxidant system was accompanied by increased expression of tumor necrosis factor-alpha (TNF-alpha), Bcl-2-associated X protein (Bax), and caspase-3. CP also caused degenerative and necrotic changes in cardiac tissues and increased cardiac biomarkers, such as serum creatine kinase, creatine kinase myocardial band, lactate dehydrogenase, and troponin I. In contrast, the Lactobacillus probiotic administration significantly reduced (p < 0.01) malondialdehyde (1.04 +/- 0.21) and NO (56.33 +/- 5.39) levels in the Lactobacillus + CP group compared with those in the CP group, and significantly increased (p < 0.01) GSH levels (2.33 +/- 0.14) in the Lactobacillus + CP group compared with that in the CP group. Lactobacillus probiotic also decreased TNF-alpha, 8-OHdG, Bax, and caspase-3 expression, and increased the expression of B-cell lymphoma 2. This enhanced the histopathology of the heart and positively affected cardiac biomarkers. In conclusion, the probiotic Lactobacillus reversed cardiotoxicity by suppressing oxidative stress, attenuating the inflammatory process, and improving the proteins that regulate apoptosis. Our findings support the use of probiotics to treat cardiotoxicity resulting from cancer therapy with CP.Article Citation - WoS: 5Citation - Scopus: 5Medicinal Evaluation and Molecular Docking Study of Osajin as an Anti-Inflammatory, Antioxidant, and Antiapoptotic Agent Against Sepsis-Associated Acute Kidney Injury in Rats(Taylor & Francis Ltd, 2024) Alhilal, Mohammad; Erol, Huseyin Serkan; Yildirim, Serkan; Cakir, Ahmet; Koc, Murat; Alhilal, Suzan; Halici, Mesut BunyamiDespite efforts to find effective drugs for sepsis-associated acute kidney injury (SA-AKI), mortality rates in patients with SA-AKI have not decreased. Our study evaluated the protective effects of isoflavone osajin (OSJ) on SA-AKI in rats by targeting inflammation, oxidative stress, and apoptosis, which represent the cornerstones in the pathophysiological mechanism of SA-AKI. Polymicrobial sepsis was induced in rats via the cecal ligation and puncture (CLP) technique. Markers of oxidative stress were evaluated in kidney tissues using biochemical methods. The expression of interleukin-33 (IL-33), 8-hydroxydeoxyguanosine (8-OHdG), caspase-3, and kidney injury molecule-1 (KIM-1) was evaluated as indicators of inflammation, DNA damage, apoptosis, and SA-AKI respectively in the kidney tissues using immunohistochemical and immunofluorescent detection methods. The CLP technique significantly (p < 0.001) increased lipid peroxidation (LPO) levels and significantly (p < 0.001) decreased the activities of superoxide dismutase and catalase in kidney tissues. In the renal tissues, strong expression of IL-33, 8-OHdG, caspase-3, and KIM-1 was observed with severe degeneration and necrosis in the tubular epithelium and intense interstitial nephritis. In contrast, the administration of OSJ significantly (p < 0.001) reduced the level of LPO, markedly improved biomarkers of antioxidant status, decreased the levels of serum creatinine and urea, lowered the expression of IL-33, 8-OHdG, caspase-3, and KIM-1 and alleviated changes in renal histopathology. A promising binding score was found via a molecular docking investigation of the OSJ-binding mode with mouse IL-33 (PDB Code: 5VI4). Therefore, OSJ protects against SA-AKI by suppressing the IL-33/LPO/8-OHdG/caspase-3 pathway and improving the antioxidant system.Article Citation - WoS: 11Citation - Scopus: 12Eco-Friendly Synthesis of Thiazole Derivatives Using Recyclable Cross-Linked Chitosan Hydrogel Biocatalyst Under Ultrasonic Irradiation as Anti-Hepatocarcinogenic Agents(Mdpi, 2024) Gomha, Sobhi M.; Abd El-Ghany, Nahed A.; Ebaid, Manal S.; Abolibda, Tariq Z.; E. A. Zaki, Magdi; Alhilal, Mohammad; Mohamed, Nadia A.; El-Ghany, Nahed A. AbdIn the current study, pyromellitimide benzoyl thiourea cross-linked chitosan (PIBTU-CS) hydrogel, was evaluated as a green biocatalyst for the efficient synthesis of novel thiazole derivatives. The PIBTU-CS hydrogel showcased key advantages, such as an expanded surface area and superior thermal stability, establishing it as a potent eco-friendly catalyst. By employing PIBTU-CS alongside ultrasonic irradiation, we successfully synthesized a series of novel thiazoles through the reaction of 2-(4-((2-carbamothioylhydrazineylidene)methyl)phenoxy)-N-(4-chlorophenyl)acetamide with a variety of hydrazonoyl halides (6a-f) and alpha-haloketones (8a-c or 10a,b). A comparative analysis with TEA revealed that PIBTU-CS hydrogel consistently delivered significantly higher yields. This synthetic strategy provided several benefits, including mild reaction conditions, reduced reaction times, and consistently high yields. The robustness of PIBTU-CS was further underscored by its ability to be reused multiple times without a substantial reduction in catalytic efficiency. The structures of the synthesized thiazole derivatives were meticulously characterized using a range of analytical techniques, including IR, 1H-NMR, 13C-NMR, and mass spectrometry (MS), confirming their successful formation. These results underscore the potential of PIBTU-CS hydrogel as a sustainable and recyclable catalyst for the synthesis of heterocyclic compounds. Additionally, all synthesized products were tested for their anticancer activity against HepG2-1 cells, with several new compounds exhibiting good anticancer effects.Article Citation - WoS: 10Citation - Scopus: 9Osajin from Maclura pomifera alleviates sepsis-induced liver injury in rats: biochemical, histopathological and immunohistochemical estimation(Taylor & Francis Online, 2023) Alhilal, Mohammad; Huseyin Serkan Erol, Serkan Yildirim, Ahmet Cakir, Murat Koc, Demet Celebi, Mesut Bunyami Halici; Koc, Murat; Cakir, Ahmet; Erol, Huseyin Serkan; Celebi, Demet; Yildirim, Serkan; Halici, Mesut BunyamiThis paper aimed to examine the impact of flavonoid osajin (OSJ) on liver damage induced by sepsis. A total of 30 male rats were divided into 5 groups (Sham, sepsis, OSJ 150, OSJ 300 and reference). During sepsis, elevated lipid peroxidation (LPO) level and catalase activity (CAT) and decreased glutathione (GSH) level and superoxide dismutase (SOD) activity were observed in hepatic tissues of sepsis group in comparison with Sham group. A strong interleukin-33 and caspase-3 expressions were detected in hepatic tissues of sepsis group. On the contrary, OSJ administration to OSJ 300 group showed a significant decrease (P < 0.001) in LPO level (176±2.926) and significant increase (P < 0.001) in GSH level (10.586±0.083) and SOD activity (29.152±0.094) in comparison with sepsis group (185.777±1.735, 8.246±0.124, 24.307±0.379 respectively). In addition, the consumption of OSJ reduced expressions of interleukin-33 and caspase-3 and improved histopathological integrity. In conclusions, OSJ has hepatoprotective effect against sepsis-induced liver injury.Article Estradiol and Ascorbic Acid Alleviate Malathion-Induced Lung Damage in Albino Wistar Rats: a Histopathological Study(2024) Alhılal, Mohammad; Salem, Mahmoud Elsayed MohamedAim: To assess the modulatory role of estradiol and ascorbic acid in malathion-induced pulmonary toxicity in albino Wistar rats. Methods: A total of twenty albino Wistar rats were randomly divided into four groups; the control group (group 1) was given corn oil alone, the test group (group 2) received a daily dose of malathion 20 mg/kg in corn oil, treatment group A (group 3) was administered a daily dose of malathion 20 mg/kg in corn oil plus estradiol 40 μg/100 g (gram), and treatment group B (group 4) received a daily dose of malathion 20 mg/kg in corn oil plus ascorbic acid 100 mg/kg. Experimental rats were administered once daily for four weeks. The lungs were examined histopathologically using two staining methods (Hematoxylin and Eosin, Masson Trichrome). Results: There were significant reductions in degeneration, interstitial pneumonia and interstitial fibrosis for group 3 (treatment group A) compared to group 2 (test group) (p<0.05). These reductions were more statistically significant for group 4 (treatment group B) compared to group 2 (test group) (p<0.01). Therefore, the damage was less pronounced and injury severity was moderate in group 3 treated with estradiol. Group 4, with ascorbic acid, showed the most improvement with significant tissue repair under microscopic examination and mild injury compared to group 3. Conclusions: The results of our present study suggest that both estradiol and ascorbic acid have clear protective effects against malathion-induced lung injury. However, ascorbic acid exhibited more pronounced protective effects compared to estradiol. With more comprehensive studies, the positive effects of ascorbic acid and estradiol can be used to prevent lung damage in individuals exposed to malathion.Article Citation - WoS: 3Citation - Scopus: 3Biological Evaluation and Molecular Docking Studies of Novel Aza-Acyclic Nucleosides as Putative Antimicrobial, Anticancer, and Antioxidant Agents(BioMed Central Ltd, 2025) Alhilal, M.; Alhilal, S.; Gomha, S.M.; Farag, B.; Sabancilar, I.; Ouf, S.A.This study aimed to synthesize new aza-acyclic nucleosides (aza-acyclovir) and evaluate the efficacy of these synthetic compounds as potential antimicrobial, anticancer, and antioxidant agents. We prepared two novel aza-acyclic nucleosides via two reactions. The first reaction involved trichloroisocyanuric acid and dibenzosulphonyl diethylamine, and the second reaction involved trichloroisocyanuric acid and diethanolamine. We then used one-dimensional nuclear magnetic resonance (NMR) spectroscopy, two-dimensional NMR spectroscopy, infrared spectroscopy, and mass spectrometry to determine the structures of the resulting compounds. In this regard, we first tested the antimicrobial activity of these compounds against various bacteria, including Bacillus cereus, B. subtilis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa, and against fungal pathogens, including Aspergillus fumigatus, Candida tropicalis, and Alternaria solani. Next, the precise mode for the interaction between synthesized aza-acyclic nucleosides and the target protein 8HQ5 was elucidate using molecular docking analysis. Subsequently, we tested the synthesized compounds for putative anticancer activity at different concentrations (i.e., 12.5, 25, 50, 100, and 200 µg/mL) against A549 cell (Human epithelial lung carcinoma) and human umbilical vein endothelial cell (HUVEC) lines. In addition, compounds antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl-based and cupric reducing antioxidant capacity-based methods at different concentrations (i.e., 31.25, 62.5, 125, 250, and 500 µg/mL). Results revealed that both aza-acyclic nucleosides inhibited both bacterial and fungal strains, although toxicity toward bacterial strains was generally greater than toward fungal strains. We also observed that the molecular docking results were consistent with the results of in vitro antimicrobial assessments. Further, both aza-cyclic nucleosides exhibited cytotoxic effects against both the A549 cell and HUVEC lines. Despite exhibiting lower radical scavenging activity than ascorbic acid (an antioxidant compound used as a standard), Compound 1 from the novel synthetic aza-acyclic nucleosides showed a higher reduction capacity, which was dose-dependent. Overall, we report newly synthesized compounds that show promising antimicrobial, anticancer, and antioxidant effects. © 2025 Elsevier B.V., All rights reserved.Article Morin Hydrate Prevents Diabetic Nephropathy by Suppressing Oxidative Stress and 8-Hydroxydeoxyguanosine in Kidney Tissues(2025) Alhılal, Mohammad; Yildirim, Serkan; Kiliçlioğlu, Metin; Alhilal, Suzan; Esra, Dereli; Yıldırım, Hüseyin ErenAims: The aim of this study was to investigate the protective effect of morin hydrate either individually or in combination with metformin against diabetic nephropathy by targeting oxidative stress and 8-hydroxydeoxyguanosine (8-OHdG) in the kidney tissue of rats with diabetic nephropathy. Methods: In this experimental study, diabetic nephropathy was induced in rats by injection of streptozotocin (STZ). The ability of morin hydrate to inhibit diabetic nephropathy was tested by screening lipid peroxidation (LPO), glutathione, glutathione peroxidase, superoxide dismutase, and catalase as parameters of oxidative stress; 8-OHdG as a marker of DNA damage and kidney injury molecule-1 (KIM-1) and aquaporin as indicators of kidney injury in renal tissues; and serum creatinine and blood urea nitrogen as markers of renal function using biochemical, immunohistochemical, and immunofluorescence methods. Results: Significant increases (p<0.0001) in LPO, 8-OHdG, KIM-1, and aquaporin levels and significant decreases (p<0.0001) in glutathione, glutathione peroxidase, superoxide dismutase, and catalase levels were observed after STZ administration, indicating the development and progression of diabetic nephropathy. Treatment with morin hydrate, especially in combination with metformin, suppressed the oxidant levels and improved the antioxidant system and the histopathological integrity of the kidney, which was positively reflected in the levels of KIM-1, aquaporin, and kidney function parameters. Conclusion: Morin hydrate prevents diabetic nephropathy resulting from diabetes mellitus by suppressing oxidative stress and 8-OHdG levels in kidney tissues. Therefore, this bioflavonoid represents a promising candidate for patients with diabetic nephropathy. Moreover, the combination therapy of morin hydrate and metformin achieved better effectiveness than the single treatment, which emphasizes an important synergistic role of morin hydrate and metformin in managing patients with diabetic nephropathy.Article Citation - WoS: 28Citation - Scopus: 29Novel thiadiazole-thiazole hybrids: synthesis, molecular docking, and cytotoxicity evaluation against liver cancer cell lines(Taylor & Francis Online, 2022) Aljohani, Ghadah F.; Abolibda, Tariq Z.; Alhilal, Mohammad; Al-Humaidi, Jehan Y.; Alhilal, Suzan; Ahmed, Hoda A.; Gomha, Sobhi M.One of the worst diseases, cancer claims millions of lives each year throughout the world, necessitating the creation of novel treatments. In this study, we designed a novel series of 1,3,4-thiadiazoles through the reaction of 2-(4-methyl-2-(2-(1-phenylethylidene)hydrazineyl)thiazole-5-carbonyl)-N-phenylhydrazine-1-carbothioamide (3) with the proper hydrazonoyl halides. Using the MTT assay, the newly synthesized thiadiazoles' growth-inhibitory potential against the liver cancer cell line HepG2-1 was assessed. In comparison to the standard drug doxorubicin (IC50 = 0.72 ± 0.52 µM), the results showed that two compounds, 16b and 21 (IC50 = 0.69 ± 0.41 and 1.82 ± 0.94 µM, respectively) had promising anticancer activity. The structural activity relationship (SAR) was investigated. In addition, molecular docking analysis onto quinone oxidoreductase2 (NQO2) receptor (PDB: 4ZVM) was investigated against the potent compounds to examine the reliability of the in vitro results. The newly prepared thiadiazole-thiazole hybrids are therefore regarded as potent anticancer drugs.
