Investigating Antimicrobial Activity of Silver Nanoparticles Produced Through Green Synthesis Using Leaf Extract of Common Grape (Vitis Vinifera)

Abstract

In this study, a direct approach to fabricating silver nanoparticles (AgNPs) via the leaf extract of common grape (Vitis vinifera) has been demonstrated. The produced particles were found with a maximum wavelength of 452.47 nm, spherical shape and the crystal size of 18.53 nm through UV-Visible spectrophotometry, XRD (X-ray diffraction) and SEM (Scanning electron microscopy) characterization methods. Furthermore, the functional groups involved in the reduction were specified with FTIR (Fourier transform infrared spectroscopy), the elemental compounds were identified with EDX (Energy dispersive X-Ray spectroscopy) and the degradation points were determined with TGA-DTA (Thermal gravimetric analysis) methods. AgNPs were found to be effective against hospital pathogens, namely Gram-negative Escherichia coli ATCC 25922, Gram-positive Staphylococcus aureus ATCC 29213 and Candida albicans fungus at the concentrations of 0.314, 0.078 and 0.334 mu g mL(-1), respectively.