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Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring

dc.authorid 0000-0002-2097-7818
dc.contributor.author Erdem Güzel, Elif
dc.contributor.author Nalan Kaya, Ahmet Tektemur, Nazife Ulker, Ahmet Yardimci, Ramazan Fazil Akkoc, Sinan Canpolat & Ibrahim Enver
dc.contributor.other Department of Midwifery/ Ebelik Bölümü
dc.date.accessioned 2021-06-29T10:11:21Z
dc.date.available 2021-06-29T10:11:21Z
dc.date.issued 2020
dc.department MAÜ, Fakülteler, Sağlık Bilimleri Fakültesi, Ebelik Bölümü en_US
dc.description.abstract Prenatal tobacco-smoke exposure negatively affects the reproductive functions of female offspring and oxidative stress plays a major role at this point. Alpha-lipoic acid (ALA), well known as a biological antioxidant, has been used as a nutritional supplement and as a therapeutic agent in the treatment of certain complications during pregnancy. We aimed to investigate the effects of maternal tobacco-smoke exposure and/or ALA administration on puberty onset, sexual behavior, gonadotrophin levels, apoptosis-related genes, apoptotic cell numbers and oxidative stress markers in the adult female rat offspring. Sprague-Dawley rats were divided into four groups; control, tobacco smoke (TS), TS+ALA and ALA groups. Animals were exposed to TS and/or ALA for 8 weeks before pregnancy and throughout pregnancy. All treatments ended with birth and later newborn female rats were selected for each experimental group. The experiment ended at postnatal day 74-77. Maternal tobacco smoke advanced the onset of puberty in the female offspring of the TS group (p < 0.05). In all treatment groups; the mean number of anogenital investigations and lordosis quality scores showed a decline, serum luteinizing hormone levels significantly increased (p < 0.05) and several histopathological changes in ovaries were observed compared to the control group. In addition, an increase in apoptotic marker levels and apoptotic cell numbers was detected in the ovaries of all treatment groups. Decreased TAS and increased TOS levels were detected in all treatment groups compared to control. These findings suggested that maternal tobacco smoke and/or ALA administration may be leading to the impaired reproductive health of female offspring. Abbreviations: ALA: alpha-lipoic acid; LH: luteinizing hormone; FSH: follicle-stimulating hormone; TAS: total antioxidant status; TOS: total oxidant status; Apaf1: apoptotic protease-activating factor 1; Casp3: caspase 3; Casp9: caspase 9; CF: cyst follicles; 4-HNE: 4-Hidroxynonenal; 8-OHdG: 8-hydroxydeoxyguanosine; TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine-biotin nick end labeling; ROS: reactive oxygen species; GnRHR: gonadotropin-releasing hormone receptor; HPG: hypothalamic-pituitary-gonadal; AMPK: AMP-activated protein kinase; ELISA: enzyme-linked immunosorbent assay; cDNA: complementary DNA; qPCR: quantitative real-time PCR; FC: follicular cysts; PF: primary follicle; SF: secondary follicle; GF: graafian follicle; CL: corpus luteum; DF: degenerated follicle; AF: atretic follicle. en_US
dc.description.citation Erdem Guzel E, Kaya N, Tektemur A, Ulker N, Yardimci A, Akkoc RF, Canpolat S, Ozan IE. Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring. Syst Biol Reprod Med. 2020 Dec;66(6):387-399. doi: 10.1080/19396368.2020.1815248. Epub 2020 Sep 20. PMID: 32951465. en_US
dc.identifier.doi 10.1080/19396368.2020.1815248
dc.identifier.endpage 399 en_US
dc.identifier.issue 6 en_US
dc.identifier.pmid 32951465
dc.identifier.scopus 2-s2.0-85091203470
dc.identifier.startpage 387 en_US
dc.identifier.uri https://pubmed.ncbi.nlm.nih.gov/32951465/
dc.identifier.uri https://doi.org/10.1080/19396368.2020.1815248
dc.identifier.uri https://hdl.handle.net/20.500.12514/2633
dc.identifier.volume 66 en_US
dc.identifier.wos WOS:000571277300001
dc.identifier.wosquality Q3
dc.indekslendigikaynak Web of Science en_US
dc.indekslendigikaynak Scopus en_US
dc.indekslendigikaynak PubMed en_US
dc.language.iso en en_US
dc.publisher Taylor & Francis Online en_US
dc.relation.ispartof Systems Biology in Reproductive Medicine en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 3
dc.subject Maternal tobacco smoke exposure; alpha-lipoic acid; puberty onset; rat female offspring; sexual behavior. en_US
dc.title Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring en_US
dc.type Article en_US
dc.wos.citedbyCount 3
dspace.entity.type Publication
relation.isAuthorOfPublication 57c0745c-c747-4e7b-84c6-ffbb555b64ee
relation.isAuthorOfPublication.latestForDiscovery 57c0745c-c747-4e7b-84c6-ffbb555b64ee
relation.isOrgUnitOfPublication b6765cfc-da7f-42f2-91ed-1e4da13e21cc
relation.isOrgUnitOfPublication.latestForDiscovery b6765cfc-da7f-42f2-91ed-1e4da13e21cc

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