Green biosynthesis of silver nanoparticles using Prunus cerasifera pissardii nigra leaf and their antimicrobial activities against some food pathogens
Date
2022
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Publisher
Czech Journal of Food Sciences
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Abstract
In this study, silver nanoparticles (AgNPs) were synthesised using the Prunus cerasifera pissardii nigra
(PC) leaf extract in an easy, low-cost and environmentally friendly way. According to the ultraviolet-visible (UV-vis)
spectrophotometer analysis data, the nanocrystals demonstrated a characteristic peak at 456 nm. Scanning electron
microscopy (SEM), field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM)
and energy-dispersive X-ray (EDX) spectroscopy analyses revealed that the morphological structures of the biosynthesised AgNPs were mostly spherical. According to the results of X-ray diffraction (XRD) analysis, it was determined
that the crystal structures of AgNPs were cubic. The size of the nanoparticles was calculated as 23.60 nm using the
Debye-Scherrer equation. The zeta potential of the synthesised nanomaterial was measured as –15.5 mV. The minimum
inhibitory concentration (MIC) values of AgNPs on Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922,
Bacillus subtilis ATCC 11774, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 10231 were determined to be 0.062, 0.250, 0.125, 0.500 and 0.125 µg mL–1, respectively
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Keywords
AgNPs; field emission scanning electron microscopy; inhibitory concentration; Fourier transform infrared; transmission electron microscopy
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Source
Czech Journal of Food Sciences
Volume
40
Issue
5
Start Page
383
End Page
391