Detection of Trichomonas Vaginalis in Vaginal Speciemens From Women by Wet Mount, Culture and Pcr

dc.contributor.author Culha, Gulnaz
dc.contributor.author Gungoren, Arif
dc.contributor.author Demir, Cemil
dc.contributor.author Hakverdi, Ali Ulvi
dc.contributor.author Duran, Nizami
dc.date.accessioned 2025-02-15T19:37:52Z
dc.date.accessioned 2025-09-17T14:28:08Z
dc.date.available 2025-02-15T19:37:52Z
dc.date.available 2025-09-17T14:28:08Z
dc.date.issued 2015
dc.description.abstract Aim: Trichomoniasis, a sexually transmitted infection (STI) caused by Trichomonas vaginalis, affects 180 million people worldwide and causes significant morbidity. Infection with T. vaginalis has been associated with vaginitis, exocervicitis, and urethritis in women. Material and Method: In this study, we aim to investigate the presence of T. vaginalis by using three different methods for comparing the results. Two hundred T. vaginalis isolates taken from swap samples were collected in Medical Faculty, Department of Gynecology, Mustafa Kemal University Polyclinic, and examined genotypically and phenotypically to identify T. vaginalis in Parasitology Department. This research is unique in terms of its contribution to patient treatment, being the first molecular study in Turkey/Hatay to determine Trichomonas (TV) genes stemming from Trichomonas vaginalis strains.R esult: 56 out of 200 patients examined were identified as positive and 24 (42.8%) of these were identified through microscopy, 18 (32,1%) with culture and 24 (42,8%) with PCR. The number of those identified through all these methods is 14 (25%). In this study, difference was calculated using three methods (p=0.022) with Cochran's Q test. When compared with McNemar two by two, no superiority in T. vaginalis diagnosis was found between microscopy and culture (p=0.5), microscopy and PCR (p=0.063), or culture and PCR (p=0.25) methods. Discussion: Culture method is not used in routine laboratory procedures and has contamination risk. PCR method shows directly the parasite of DNAs, and so it is thought to be more reliable compared to the other two methods. en_US
dc.identifier.citationcount 3
dc.identifier.doi 10.4328/JCAM.2158
dc.identifier.issn 1309-0720
dc.identifier.issn 1309-2014
dc.identifier.scopus 2-s2.0-84929144453
dc.identifier.uri https://doi.org/10.4328/JCAM.2158
dc.identifier.uri https://hdl.handle.net/20.500.12514/9417
dc.language.iso en en_US
dc.publisher derman Medical Publ en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.subject Trichomonas Vaginalis en_US
dc.subject Microscopy en_US
dc.subject Culture en_US
dc.subject Pcr en_US
dc.title Detection of Trichomonas Vaginalis in Vaginal Speciemens From Women by Wet Mount, Culture and Pcr en_US
dc.title.alternative Trichomonas Vaginalis Tanısında Mikroskopi, Kültür ve Pcr Yöntemlerinin Karşılaştırılması en_US
dc.type Article en_US
dspace.entity.type Publication

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