Browsing by Author "Aktepe, Necmettin"

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Citation - WoS: 6
Citation - Scopus: 5
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Citation - WoS: 0
Analysis of Bioactive Compounds Using Lc-esi-ms/Ms, Cytotoxic, Antimicrobial Effects, and Enzyme Activities From Cyclotrichium Origanifolium (Vol 101, Pg 740, 2023)
(Wiley, 2024) Aktepe, Necmettin; Keskin, Cumali; Atalar, M. N.; Baran, F.; Baran, Ayşe; Taskin, A.; Jahan, I; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Citation - WoS: 5
Citation - Scopus: 5
Biochemical components, enzyme inhibitory, antioxidant and antimicrobial activities in endemic plant Scilla mesopotamica speta
(Wiley, 2021) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Akmeşe, Şükrü; Akmeşe, Şükrü; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
In this study, in vitro antioxidant, antimicrobial, anticholinesterase and phenolic profile of different solvent extracts of Scilla mesopotamica speta were determined in detail. In vitro antioxidant activities and total phenolic and flavonoid contents of plant extracts obtained with different solvents were tested in terms of 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities. The highest total phenolic and total flavonoid contents were determined in the ethyl acetate extract (62.24 mu g GAE/mg) and chloroform extract (87.72 mu g QE/mg) respectively. The highest DPPH radical scavenging activity was detected in ethyl acetate extracts. Antimicrobial and antifungal activities were investigated by MIC method. The inhibitory activities of the extracts on the acetyl cholinesterase enzyme were investigated. Liquid chromatography (LC) tandem mass spectrometry LC-MS/MS was used to determine the phenolic component content of extracts. Thirty-one different components were identified in the analyses and their amounts were measured. Practical applications Scilla mesopotamica speta is an endemic and medicinal plant. It was determined that the extracts of this plant had a very rich content in terms of phenolic compounds, especially caffeic and ferulic acids. However, this plant was remarkable for its antioxidant, anticholinesterase, and antimicrobial activities. Considering the strong antioxidant, antimicrobial, and enzyme inhibition activities of the Scilla mesopotamica speta it can be suggested as a source of anticancer, antimicrobial, and antiviral drugs.
Citation - WoS: 5
Citation - Scopus: 4
Biosynthesis of AgNPs by extract from waste leaves of Citrullus lanatus sp. (watermelon); characterization, antibacterial and antifungal effects
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe; Department of Nursing / Hemşirelik Bölümü
Silver nanoparticles (AgNPs) are valuable materials with a large number of sectors used. Green synthesis is very important for biomedical applications as they show biocompatible properties. In this study, AgNPs were easily synthesized using the environmentally friendly green synthesis approach using agricultural waste parts of Citrullus lanatus sp. plant grown in Diyarbakır region. Characterization of synthesized AgNPs was made. Fourier Transform Infrared Spectroscopy (FTIR) analysis was used to evaluate the phytochemicals responsible for effective reduction in the formation of AgNPs. UV-visible spectrophotometer (UV-Vis.) Spectra were also used to determine the presence of AgNPs. X-Ray Diffraction Diffractometer (XRD), Scanning Electron Microscope (SEM), Transmission Electron Microscope, Zeta potential analyzes were performed to define the crystal structures, dimensions and surface charges of AgNPs, respectively. In these data, it was determined that AgNPs showed maximum absorbance at 460 nm, spherical appearance, 21.27 crystal nano size and -30.05 mV zeta potential. Antimicrobial effects of AgNPs on gram positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) bacteria, gram negative Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa) bacteria, as well as on fungus C. albicans pathogenic microorganisms It was analyzed by specifying the Minimum Inhibition Concentration (MIC) by microdilution.
Cyclotrichium origanifolium L. bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi
(Mardin Artuklu Üniversitesi, 2021) Aktepe, Necmettin; Aktepe, Necmettin; Department of Nursing / Hemşirelik Bölümü
Cyclotrichium origanifolium, Lamiaceae familyasaına ait bir bitki olup, ülkemizde Batı ve Güney Anadolu bölgelerinde yetişen bir türdür. Bu tez çalışmasında Cyclotrichium origanifolium bitkisinden biyolojik yolla elde edilen gümüş nanopartiküllerin (AgNP) antimikrobiyal aktiviteleri incelendi. Araştırmanın ilk bölümünde doğal ortamından toplanan bitki örneklerinden elde edilen özütten AgNP'ler başarılı bir şekilde üretildi. Elde edilen AgNP'lerin karakterizasyon işlemleri uygun spektral analiz yöntemleri; Uv-Vis, FT-IR, SEM-EDX, XRD ile belirlendi. Analiz sonuçlarına göre AgNP'lerin küresel şekilli ve ortalama 17,60 nm çapında olduğu tespit edildi. AgNP'lerin antimikrobiyal etkisi MİK yöntemi ile belirlendi. Test mikroorganizmaları olarak gram pozitif; S. aureus, B. subtilis ve gram negatif; E. coli, P. aeruginosa bakterileri ile C. albicans fungal patojen türleri kullanıldı. Mikroorganizma gelişimlerinin baskılanması, çalışmamızda kullanılan standart antibiyotikler ile yeşil sentez yöntemle üretilen AgNP'lerin etkinlikleri karşılaştırılarak incelendi. Sonuç olarak Cyclotrichium origanifolium bitkisinden üretilen AgNP'lerin antimikrobiyal aktivitesi, standart antibiyotiklere göre daha güçlü etki gösterdiği gözlendi.
Citation - WoS: 0
Citation - Scopus: 0
Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract
(Springer int Publ Ag, 2024) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Atalar, Mehmet Nuri; Keskin, Cumali; Khalilov, Rovshan; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Purpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.
Citation - WoS: 19
Citation - Scopus: 21
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Keskin, Cumali; Aktepe, Necmettin; Baran, Ayşe; Baran, Mehmet Fırat; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Farklı Biyosorbentler Kullanarak Su Ortamında Ağır Metallerin Biyosorbsiyon Metodu ile Giderilmesi
(2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe; Baran, Ayşe; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Su canlılar için ekosistemin önemli parçasıdır. Endüstrileşme ile birlikte su kaynaklarındaki kirlenme endişe edilir boyutlara ulaşmıştır. Ağır metallere bağlı su kirliliği ve artan konsantrasyonları su ekosistemlerine verdiği zarar nedeniyle araştırmacıların konuya olan ilgisinin artmasına sebep olmuştur. Ağır metallerin sularda oluşturduğu kirliliği gidermek ciddi maliyet ve zaman gerektirmektedir. Son yıllarda suda bulunan ağır metallerin uzaklaştırılması için bakteriler kullanılarak biyosorbsiyon yönteminin kullanılması yaygınlaşmıştır. Bu yöntemin tercih edilmesinin temel sebebi gram pozitif bakterilerin hücre duvarında kalın bir peptidoglikan tabakasına sahip olması ile adsorbsiyon kapasitesini artırmasıdır. Bu çalışmada içme, atık, nehir suları ve suni olarak hazırlanan numunelerde çalkalamalı metod kullanarak ağır metal biyosorbsiyonu ile birlikte çoklu hazırlanmış ağır metal çözeltilerinde biyosorbsiyon rekabeti incelenmiştir. Bu işlemler için Dicle nehri bölgesine ait topraklardan izole edilen Bacillus licheniformis sp. Bacillus subtilis sp. ve Bacillus subtilis (ATCC 6051) suşları ile sulu çözeltiden Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metallerinin biyosorbsiyonu değerlendirildi. B. subtilis suşları ve B.licheniformis sp. organizmalarının yüzey morfoljik yapıları SEM görüntüleri, element kompozisyonları EDAX verileri ile incelendi. ICP-OES kullanılarak element içeriği tespit edildi. Sulu çözeltideki Cd (II), Cu (II), Pb (II), Fe (II), Ni (II) ve Zn (II) metal iyonları farklı pH’larda 25 °C de 0,25 mg L-1 biomass ile biyosorbsiyon gerçekleştirildi. pH 6.0 da maksimum biyosorbsiyon ile metal iyonlarının % 98 varan oranda giderildiği belirlendi.
Citation - WoS: 11
Citation - Scopus: 10
Fast and Low-Cost Biosynthesis of AgNPs with Almond Leaves: Medical Applications with Biocompatible Structures
(Progress in Nutrition, 2021) Aktepe, Necmettin; Baran, Ayşe; Department of Nursing / Hemşirelik Bölümü
In this study, silver nanoparticles (AgNPs) were obtained in a low cost, easy and simple way by using the leaf extract of Prunus dulcis L. (almond tree) growing in the Mardin region. Characterization of AgNPs obtained by biosynthesis: Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffractometer (XRD), UV-visible spectrophotometer (UV-Vis.), Scanning Electron Microscope (SEM), Transmission Electron Microscope(TEM), Zeta potential and Zeta size analysis was done through data. It was determined that AgNPs have maximum absorbance at 443 nm wavelength, they exhibit 14.67 crystal nano size, -19.9 mV zeta potential in spherical appearance. The Minimum Inhibition Concentration (MIC) of the obtained AgNPs was determined by using microdilution method on the growth of pathogen strains.
Green synthesis and antimicrobial effects of silver nanoparticles by pumpkin cucurbita maxima fruit fiber
(Medicine Science, 2022) Aktepe, Necmettin; Baran, Ayşe; Department of Nursing / Hemşirelik Bölümü
In the present study, for the first time, green synthesis of silver nanoparticles was carried out with the extract obtained from the fibrous structure in the pumpkin Cucurbita maxima (CM) fruit. The presence of silver nanoparticles formed by the reduction of Ag+ ions was determined with the maximum absorbance values of 442.89 nm in wavelength scans made by UV-Vis spectroscopy analysis. The functional groups of the phytochemicals involved in the reduction were examined by Fourier-transform infrared (FT-IR) spectroscopy. For the morphology of the synthesized AgNPs, they were found to be uniform in spherical appearance using atomic power microscopy (AFM), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) images. The surface charges of AgNPs were determined to be -16.4 mV by Zeta potential analysis. Their crystal structures and nano-sizes were evaluated by X-ray diffraction (XRD) data to be in cubic pattern, and their size was 21.20 nm using the Debye-Scherrer equation. AgNPs (CM-AgNPs) synthesized via CM provided growth suppression at concentrations of 1.00-0.12, 2.00-1.00, and 0.50 µg/mL on pathogen gram-positive, gram-negative strains and the fungus Candida albicans, respectively. These concentrations were effective against antibiotics and silver solution at a very low concentration.
Gümüş Nano Materyallerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktiviteleri
(2021) Aktepe, Necmettin; Department of Nursing / Hemşirelik Bölümü
Gümüş nano materyallerin kullanım alanları oldukça yaygındır. Farklı sentezleme yöntemleri ile elde edilebilirler. Bu çalışmada, gümüş nano materyaller (AgNP’ler) biyosentez yöntemi kullanılarak ekonomik ve basit bir şekilde elde edildi. Sentez sonucunda elde edilen AgNP’ler UV-visiblespektrofotometre (UV-Vis.), Taramalı Elektron Mikroskobu (SEM), X- Işınımı Kırınımı Difraktrometresi (XRD), Fourier dönüşümü kızılötesi spektroskopisi (FTIR), Zeta potansiyeli cihazları kullanılarak karakterize edildi. AgNP’lerin 426.66 nm dalga boyunda maksimum absorbansa sahip oldukları, küresel morfolojik yapı sergiledikleri, 17.68 nm kristal nano boyut ve yüzey yüklerinin -20.9 mV zeta potansiyeli dağılımı gösterdiği değerlendirildi. Patojen mikroorganizmalar üzerinde Minumum İnhibisyon Konsantrasyonları (MİK) 0.03-0.5 mg/L olarak mikrodilusyon yöntemi kullanılarak tespit edildi.
Citation - WoS: 0
Hydrogen peroxide-induced oxidative damage in human mononuclear leukocyte: the anti-genotoxic effects of H. Perforatum extract on DNA damage
(WILEY-BLACKWELL, 2015) Aktepe, Necmettin; Keskin, Cumali; Yukselten, Y.; Sunguroglu, A.; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
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Citation - WoS: 17
Citation - Scopus: 14
In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes
(Iranian Journal of Pharmaceutical Research, 2017) Keskin, Cumali; Aktepe, Necmettin; Yükselten, Y.; Asuman,; Boğa, M.; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.
Citation - WoS: 21
Citation - Scopus: 21
Increased DNA Damage and Oxidative Stress Among Silver Jewelry Workers
(HUMANA PRESS INC, 2015) Aktepe, Necmettin; Keskin, Cumali; Yükselten, Yunus; Taşkın, Abdullah; Keskin, Cumali; Çelik, Hakim; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Silver has long been valued as a precious metal, and it is used to make ornaments, jewelry, high-value tableware, utensils, and currency coins. Human exposures to silver and silver compounds can occur oral, dermal, or by inhalation. In this study, we investigated genotoxic and oxidative effects of silver exposure among silver jewelry workers. DNA damage in peripheral mononuclear leukocytes was measured by using the comet assay. Serum total antioxidative status (TAS), total oxidative status (TOS), total thiol contents, and ceruloplasmin levels were measured by using colorimetric methods among silver jewelry workers. Moreover, oxidative stress index (OSI) was calculated. Results were compared with non-exposed healthy subjects. The mean values of mononuclear leukocyte DNA damage were significantly higher than control subjects (p < 0.001). Serum TOS, OSI, and ceruloplasmin levels were also found to be higher in silver particles exposed group than those of non-exposed group (p < 0.001, p < 0.001, p < 0.01, respectively). However, serum TAS levels and total thiol contents of silver exposed group were found significantly lower (p < 0.05, p < 0.001, respectively). Exposure to silver particles among silver jewelry workers caused oxidative stress and accumulation of severe DNA damage.
Citation - WoS: 2
Citation - Scopus: 2
Induction of apoptosis in human hormone-refractory prostate cancer cell lines by using resveratrol in combination with AT-101
(Wiley, 2021) Aktepe, Necmettin; Yukselten, Yunus; Department of Nursing / Hemşirelik Bölümü
The aim of this study was to determine the appropriate doses of AT-101 and resveratrol combination in the in vitro hormone-refractory prostate cancer (PC) cell lines, in order to evaluate the cytotoxic and genotoxic effects of this combination on the proliferation of cancer cells, namely PC-3, DU-145 and LNCAP. Cytotoxicity in PC cell lines was analysed by using the XTT Cell Proliferation Assay. DNA damage was performed with the cell death assay. Apoptotic protein levels were performed by Roche Human Apoptosis Array. IC50 values were determined by XTT analysis. The strongest combined doses (100 µM resveratrol + 5µM AT-101) were found to have the strongest synergistic apoptotic and cytotoxic effects on DU-145 cells at 72 hr. While the combined use of resveratrol and AT-101 increased the expression of markers in apoptotic cell pathways on cells, a decrease in the expression of anti-apoptotic markers was detected (p ˂ 0.05). Combined applications of these compounds showed an important synergism in the hormone-refractory PC cell lines, and it was determined that after the post-translational modification, they were significantly effective on the apoptotic pathway. These results have revealed that the combination of resveratrol and AT-101 holds great expectation as a new chemotherapeutic application in the treatment of human prostate cancer.
Citation - WoS: 2
Citation - Scopus: 2
Morin Attenuates Arsenic-Induced Toxicity in 3t3 Embryonic Fibroblast Cells by Suppressing Oxidative Stress, Inflammation, and Apoptosis: in Vitro and Silico Evaluations
(Oxford Univ Press, 2024) Unsal, Velid; Aktepe, Necmettin; Aktepe, Necmettin; Oner, Erkan; Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; Department of Nursing / Hemşirelik Bölümü
This study aims to investigate the curative effects of Morin, a flavonoid, against arsenic toxicity in 3T3 embryonic fibroblast cells and its effect on the molecular mechanisms of cells. The cytotoxicity and viability of the cells were measured by MTT and LDH tests. Arsenic (0.74 mu M) was used to trigger toxicity and Morin (50 mu M) was used for treatment. The levels of oxidative stress biomarkers and the activities of antioxidant enzymes were measured by spectrophotometric method, and inflammatory markers were measured by ELISA method. While mRNA expression levels of Bax, Bcl-2 levels, and Caspase-3 activity were measured by qRT-PCR technique, TUNEL staining was performed to detect DNA breaks and DAPI staining to visualize nuclear changes. Protein structures were retrieved from the protein data bank. OpenBabel and Autodock programs were used for the molecular docking study. Morin rescued the 3T3 embryonic fibroblast cells exposed to arsenic. However, Arsenic decreased the activities of antioxidant enzymes in cells and significantly increased oxidative stress, inflammation, and apoptosis. Morin treatment reduced oxidative damage and TNF-alpha and IL-1 beta levels. Arsenic-induced Caspase-3 mRNA expression level and Bax protein mRNA expression level were significantly increased, while Bcl-2 mRNA expression level was significantly decreased. While Caspase-3 mRNA expression level and Bax protein mRNA expression level decreased with morin treatment, Bcl-2 mRNA expression level increased significantly. Molecular docking study results showed good binding affinity of morin in SOD, GSH-Px, Bax, Bcl-2, Caspase-3, TNF-alpha, and IL-1 beta structures. Morin showed antioxidant, anti-inflammatory, and anti-apoptotic effects against Arsenic-induced cellular toxicity. Graphical Abstract
The protective effects of different parts of hypericum perforatum extracts on human mononuclear leukocytes in hydrogen peroxide-induced DNA damage and their phenolic contents
(Medicine Science, 2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Department of Nursing / Hemşirelik Bölümü; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
Oxidative stress is the state of the formation of some pathophysiological condition with the excessive increase of the normal amount of free radicals in the organism. In this study, the in vivo genotoxic and antigenotoxic effects of methanol and water extract and phenolic content of Hypericum perforatum flower, fruit, and seed methanol extracts were analyzed. HPLC was used to evaluate the quantities of 3,4-Dihydroxybenzoic acid, syringic acid, hydroxycinnamic acid, O-coumaric acid, caffeic acid, and catechin in the methanol extracts. The alkaline comet test was used to assess the DNA damage and protective effects of H. perforatum flower fruit, seed methanol, and water extract on human mononuclear leukocytes. The amounts of catechin and caffeic acid in seed methanol extract were found as quite high when compared to other extracts. The highest protective effects were seen at 10 and 50μg/ml concentrations of seed methanol extract. The optimum doses of fruit, flower, and seed extracts obtained from H. perforatum neutralized the genotoxic effect. This effect is stronger in seed methanol extract than other extracts. We suggest that more research is needed to evaluate the effects of H. perforatum phytochemicals in vitro and in vivo.
Citation - WoS: 0
Removal of Heavy Metals in Water by Biosorption Method Using Three Different Bacillus sp-derived Biosorbents
(Kahramanmaraş Sütçü İmam Üniversitesi, 2022) Keskin, Cumali; Aktepe, Necmettin; Baran, Mehmet Fırat; Baran, Ayşe; Aktepe, Necmettin; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Water is an important part of the ecosystem for life. With industrialization, pollution in water resources has reached a worrying level. Water pollution due to heavy metals and their increasing concentrations have caused researchers to increase their interest in the subject due to the damage they cause to water ecosystems. It requires serious cost and time to eliminate the pollution caused by heavy metals in water. In recent, years, the use of biosorption method using bacteria to remove heavy metals in water has become widespread. The main reason why this method is preferred is that gram-positive bacteria have a thick peptidoglycan layer on the cell wall and increases the adsorption capacity. In this study. in drinking, waste, river water and artificially prepared samples, batch method of heavy metal biosorption and biosorption competition in multiple prepared heavy metal solutions were investigated. For these processes. Bacillus licheniformth sp. Bacillus subtilis sp. and Bacillus subtilis (ATCC 6051) strains were used as a biosorbent. Biosorbtion of Cd (II), Cu (H), Pb (II), Fe(II), Ni (II) and Zn (II) metals from waters with these biosorbents at different pHs at. 25 degrees C with 0.25 mg LI It was investigated. Surface morphological structures of biosorbents were evaluated using SEM images and element compositions were evaluated using EDAX profile. Element content was determined using ICP-OES. It was found that heavy metal ions were removed up to 98% with maximum biosorption at pH 6.0.
Rumex acetosella l. (kuzukulağı) bitkisinden gümüş nanopartiküllerin sentezi, karakterizasyonu ve antimikrobiyal aktivitelerinin değerlendirilmesi
(Mardin Artuklu Üniversitesi, 2022) Aktepe, Necmettin; Aktepe, Necmettin; Department of Nursing / Hemşirelik Bölümü
Rumex acetosella L. (kuzukulağı), Polygonaceae familyasına mensup bir bitki olup ülkemiz genelinde doğal olarak yetişen bir türdür. Bu tez çalışmasında Rumx acetosella L. bitkisinden yeşil sentez yöntemi ile elde edilen gümüş nanopartiküllerin (AgNP) karakterizasyonu ve antimikrobiyal aktiviteleri incelenmiştir. Araştırmanın ilk aşamasında doğal yetiştiği ortamından toplanan bitki örneklerinden elde edilen bitki özütü ile AgNP'ler başarılı bir şekilde sentezlenmiştir. Elde edilen Ra-AgNP'lerin karakterizasyonu uygun analiz yöntemleri kullanılarak (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potansiyeli ve Zeta Sizer) tespit edilmiştir. Analiz sonuçlarına göre AgNP'lerin 476 nm dalga boyunda maksimum absorbans gösterdiği; beşgen, altıgen ve küresel görünüm; 29.16 nm boyut; -¬9.88 mV zeta potansiyeline sahip oldukları belirlenmiştir. AgNP'lerin antimikrobiyal aktiviteleri gram pozitif Staphylococcus aureus, Bacillus subtilis ve gram negatif Pseudomonas aeruginosa, Escherichia coli bakterileri ile Candida albicans mantarı üzerinde MİK (Minimum İnhibisyon Konsantrasyonu) değerlerinin belirlendiği mikrodilüsyon yöntemi ile tespit edilmiştir. Sonuç olarak Ra-AgNP'lerin hem düşük konsantrasyonlarda hem de ticari antibiyotiklere kıyasla daha güçlü antimikrobiyal aktivite gösterdiği belirlenmiştir.
Synthesis, Characterization and Evaluation of Antimicrobial Activities of Silver Nanoparticles Obtained From Rumex Acetosella L. (sorrel) Plant
(2022) Aktepe, Necmettin; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; Department of Nursing / Hemşirelik Bölümü
Rumex acetosella L. (sorrel) is a plant belonging to the Polygonaceous family and is a species that grows naturally across Turkey. In this study, the characterization of silver nanoparticles (AgNPs) obtained from the Rumex acetocella L. (RA) plant using the green synthesis method was performed and their antimicrobial activities were investigated. AgNPs were successfully synthesized in the first stage of the study using plant extract taken from plant samples collected from the natural growing environment. Characterization of synthesized AgNPs was performed using appropriate analytical methods (UV-vis, FT-IR, XRD, SEM-EDX, TEM, Zeta Potential and Zeta Sizer). According to the analysis results, it was determined that AgNPs had a maximum absorbance at 476 nm wavelength, a pentagonal, hexagonal, and spherical appearance, a size of 29.16 nm, and a zeta potential of -9.88 mV. The antimicrobial activities of AgNPs were tested using the microdilution technique, in which Minimum Inhibition Concentration (MIC) values were determined on gram-positive Staphylococcus aureus, Bacillus subtilis and gram-negative Pseudomonas aeruginosa, Escherichia coli bacteria and Candida albicans fungus. It showed a very strong antimicrobial effect on C. albicans, S. aureus and P. aeruginosa. Consequently, AgNPs had stronger antimicrobial activity at low concentrations and when compared to commercial antibiotics.