Tıbbi Hizmetler ve Teknikler Bölümü

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Citation - WoS: 11
Citation - Scopus: 11
Anoxybacillus sp. SO B1-Immobilized Amberlite XAD-16 for Solid-Phase Preconcentration of Cu(II) and Pb(II) and Their Determinations by Flame Atomic Absorption Spectrometry
(TAYLOR & FRANCIS INC, 2015) Oral, Elif Varhan; Ozdemir, Sadin; Dolak, Ibrahim; Okumus, Veysi; Dundar, Abdurrahman; Ziyadanogullari, Berrin; Aksoy, Zehra; Onat, Ruken
A new method for the determination of Cu(II) and Pb(II) by flame atomic absorption spectrometry (FAAS) after preconcentrating on a column containing Anoxybacillus sp. SO B1-immobilized Amberlite XAD-16 was developed. The functional groups of Anoxybacillus sp. SO B1 immobilized on Amberlite XAD-16 were characterized in KBr tablets by Fourier transform infrared (FT-IR) spectrometry. Various parameters such as pH, amount of the adsorbent, eluent type and volume, and flow rate of the sample solution were studied. The optimum pH values of quantitative sorption for Cu(II) and Pb(II) were found to be pH 7.0 and 5.0 and Cu(II) and Pb(II) ions could be quantitatively eluted with 5.0ml of 1.0mol L-1 HCI and 10.0ml of 0.25mol L-1 HNO3, respectively. Recoveries of Cu(II) and Pb(II) were found to be 100.9 +/- 1.57% and 100.3 +/- 0.49% (N = 5), the limits of detection of Cu(II) and Pb(II) in the determination by FAAS (3s, N = 10) were found to be 0.8 and 1.6 mu g L-1, respectively. The proposed enrichment method was applied for metal ion determination from water samples such as two parts of Tigris River water in Diyarbakir and Elazig, Lake of Hazar in Elazig, and tap water in Diyarbakir. Furthermore, the accuracy of the proposed method was verified by studying the analytical recovery and by analyzing certified reference material (NCS-DC 73350 leaves of poplar).
Citation - WoS: 62
Citation - Scopus: 68
Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron
(Elsevier, 2020) Cengiz, Mustafa; Şahintürk, Varol; Cetik Yildiz, Songul; Kurcanay Şahin, İlknur; Bilici, Namık; Onur Yaman, Suzan; Altuner, Yılmaz; Appak-Baskoy, Sıla; Ayhanci, Adnan
Background Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.
Citation - WoS: 7
Citation - Scopus: 10
Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C-60 modified gamma-Fe2O3 superparamagnetic iron oxide nanoparticles
(ROYAL SOC CHEMISTRY, 2015) Kılınç, Ersin
In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C-60 modified maghemite (gamma-Fe2O3 superparamagnetic iron oxide nanoparticles - SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL(-1) with a limit of detection of 0.06 ng mL(-1). The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL(-1). The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL(-1).
Citation - Scopus: 14
Dose Ginger Have Preventative Effects on PONV-Related Eye Surgery? A Clinical Trial
(Anesthesiology and pain medicine, 2019) Sedigh Maroufi, Shahnam; Moradimajd, Parisa; Moosavi, Seyed Ali Akbar; Imani, Farnad; Samaee, Hamidreza; Oguz, Mehmet
Background: Postoperative nausea and vomiting (PONV) is considered a common complication of anesthesia, which, particularly in eye surgery, may exert pressure on stitches and open or leak the surgical wound, leading to bleeding. Objectives: We aimed to study the effect of ginger on PONV and changes in vital signs after eye surgery. Methods: In this triple-blind randomized controlled trial, 120 candidate patients for eye surgery were divided into group A (n = 40) and group B (n = 40). Patients in group A received the capsules of ginger 1 g while patients in group B received a placebo one hour before the procedure with 30 mL water. The incidence of nausea and the frequency of vomiting were evaluated at 0, 15, 30 minutes, and 2 hours after the operation. Also, the vital signs of the participants were recorded at certain times. Results: The results demonstrated a statistically significant difference in the frequency of nausea between group A and group B (P < 0.05). The severity of nausea was lower in group A than in group B immediately and 2 hours after recovery (P < 0.05). The incidence of vomiting was significantly lower in group A than in group B (P < 0.05). The vital signs were not significantly different between group A and group B (P > 0.05). Conclusions: Ginger was effective in the prevention of PONV after eye surgery but had no impact on vital signs. Hence, ginger is proposed to use as a low-cost, prophylactic measure for PONV reduction.
Citation - WoS: 24
Citation - Scopus: 28
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
Citation - WoS: 2
Citation - Scopus: 5
Evaluation as time-dependent of pesticides applied in pre-harvest period of grown vegetables: removal of pesticide residues in the vegetables
(Taylor & Francis Online, 2022) Elmastas, Ayhan; Umaz, Adil; Pirinc, Vedat; Aydin, Firat
Pesticide use has benefits such as increasing the quality and amount of food required to sustain the human population. However, its harmful effects on the environment and human health are of great concern. The aim of this study was to evaluate concentrations as timedependent of the pesticide and the removal of pesticide residues in vegetable samples with the aid of the QuEChERS 2007.01 procedure. The analytical method has been validated according to SANTE/12682/2019. Good linearity (2.5–100 ng/mL, R2 > 0.99) was observed, and the recovery yields ranged from 94.76% to 104.03%, repeatability (RSDr) ranged from 2.33% to 4.59% and reproducibility (RSDwR) ranged from 4.06% to 8.40%. LOD and LOQ values were determined to be 3.00 ng/g and 10.00 ng/g, respectively. The grown tomato, eggplant and cucumber samples were sprayed with drugs against pests and diseases. Imidacloprid in the grown tomato, cucumber and eggplant samples was determined to be 0.026, 0.186 and 0.096 mg/kg, respectively. Trifloxystrobin was determined to be 0.102, 0.129 and 0.063 mg/kg, respectively. It was determined that pesticide residue in vegetable samples decreases by washing and peeling but does not disappear completely. The peeling process was determined to be more effective than washing. The concentration of pesticide residues in the shell part was found to be hight than in the whole product. Thus, it is important to consume vegetables with their skin peeled and to consume the unpeeled ones by washing them with water at least.
Citation - WoS: 39
Citation - Scopus: 43
Fabrication and characterization of Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide magnetic nanomaterials
(SpringerLink, 2022) Kutluay, Sinan; Şahin, Ömer; Ece, Mehmet Şakir
Abstract In this study, the fabrication of perlite-supported Fe3O4 (Fe3O4/perlite), SiO2-coated Fe3O4/perlite (Fe3O4/perlite@SiO2), and sulfanilamide-modified Fe3O4/perlite@SiO2 (Fe3O4/perlite@SiO2@sulfanilamide) magnetic nanomaterials and their characterization by various spectroscopic techniques were presented. For this purpose, first, Fe3O4/perlite was fabricated via the co-precipitation method. Then, Fe3O4/perlite@SiO2 and Fe3O4/perlite@SiO2@sulfanilamide nanomaterials were fabricated using the sol–gel method. The structural properties of the fabricated nanomaterials were characterized using Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy-dispersive X-ray spectroscopy (EDX), thermogravimetric analysis-differential thermal analysis, and X-ray diffraction (XRD) analyses. The SEM, SEM–EDX, FTIR, and XRD analyses revealed that the fabrication and surface coatings of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were successfully performed. It was concluded that the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide showed a type IV-H3 hysteresis loop according to the International Union of Pure and Applied Chemistry classification. According to the BET analysis, it was found that the specific surface areas of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 8.09, 12.71, and 5.89 m2/g, respectively. The average pore radius of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 9.68, 7.91, and 34.69 nm, respectively, using the Barrett-Joyner-Halenda method. Moreover, the half-pore widths of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 2.27, 1.58, and 17.99 nm, respectively, using the density functional theory method. Furthermore, in light of characterization findings, the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were in crystalline cubic spinel form, and they had mechanical and thermal stability and a mesoporous structure. Within the framework of the results, these developed nanomaterials, which have potential in many applications, such as sustainable technologies and environmental safety technologies, were brought to the attention of related fields.
Citation - WoS: 36
Citation - Scopus: 46
gamma-Fe2O3 magnetic nanoparticle functionalized with carboxylated multi walled carbon nanotube: Synthesis, characterization, analytical and biomedical application
(ELSEVIER SCIENCE BV, 2016) Kilinc, Ersin
In recent years, magnetic nanoparticles attained special interest in nanobiotechnology and nanomedicine due to their uniqe properties and biocompatibilities. From this perspective, hybride nanostructure composed from gamma-Fe2O3 magnetic nanoparticle and carboxylated multi walled carbon nanotube was synthesized and characterized by FT-IR, VSM, SEM, HR-TEM and ICP-OES. Microscopy images showed that magnetic nanoparticles were nearly spherical structure that arranged on the axis of carboxylated MWCNT. Particle size was found lower than 10 nm. VSM results showed that the obtained magnetic nanoparticles presented superparamagnetic properties at room temperature. The magnetic saturation value was determined as 35.2 emu/g. It was used for the adsorption and controlled release of harmane, a potent tremor-producing neurotoxin. Maximum adsorption capacity was calculated as 151.5 mg/g from Langmuir isotherm. Concentration of harmane was determined by HPLC with fluorescence detection. The antimicrobial activity of synthesized magnetic nanoparticle was investigated against gram-negative and gram-positive bacteria. However, no activity was observed. (C) 2015 Elsevier B.V. All rights reserved.
Citation - WoS: 18
Citation - Scopus: 18
Green synthesis and characterization of selenium nanoparticles (Se NPs) from the skin (testa) of Pistacia vera L. (Siirt pistachio) and investigation of antimicrobial and anticancer potentials
(SpringerLink, 2023) Keskin, Cumali; Mehmet Fırat Baran, Cumali Keskin, Ayşe Baran, Kadri Kurt, Polat İpek, Aziz Eftekhari, Rovshan Khalilov, Ismayil Fridunbayov, William C. Cho
Metallic nanoparticles created by ecologically friendly synthesis processes are becoming increasingly useful in a variety of applications. Because of their strong bioactive component qualities, biocompatible architectures, high stability, and low toxicity, green-produced selenium nanoparticles are particularly signifcant materials for various medicinal applications. Plants include a wealth of essential phytochemicals with therapeutic and medical capabilities. Pistachio vera L. (Siirt pistachio) is a seasonal fruit that is frequently consumed for its nutritional worth and health advantages. However, the outer colored skin of the P. vera (Siirt pistachio) fruit, which is not consumed, contains many biologically active compounds. In this study, plant-mediated synthesis of selenium nanoparticles (SeNPs) was successfully accomplished after adding the sodium selenite solution to the aqueous extract of P. vera colored skin waste. The synthesized Se NPs were characterized with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta distribution, atomic force microscope (AFM), Fourier transform infrared spectrum (FTIR), thermogravimetric (TGA) and diferential thermal analysis, X-ray difraction (XRD), and energy-dispersive X-ray spectrum (EDX). Biogenic Pistacia vera (PV) PV-SeNPs were also tested for their ability to suppress the development of various pathogenic bacteria and cancerous cell lines. The UV-visible (UV–Vis) spectra revealed an absorption peak at 325 nm, which refected the surface plasmon band. The signifcant selenium signal on the EDX spectrum at 1.5 keV confrmed the creation of Se NPs. The presence of several peaks on the FTIR spectrum of the aqueous extract of Pv and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols, which are important in facilitating the process of capping and bioreduction, as well as conferring stability to nanoparticles. The TEM microphotographs revealed that the nanoparticles were highly distributed, had a spherical morphological form, and were monodisperse below 10 nm. Biogenic Pv-SeNPs exhibited similar antimicrobial activity as standard antibiotics. However, it was determined that the cytotoxic activity of Se-NPs against cancer cell lines was quite high depending on the dose and time. As a result, the Pv-SeNPs are likely to be extremely benefcial in the pharmaceutical and cosmetic sectors, as well as in the food and cosmetic industries, in producing antimicrobial and/or anticancer medications.
Citation - WoS: 36
Citation - Scopus: 39
Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide
(Elsevier GmbH, 2019) Cengiz Mustafa; Çetik Yıldız, Songül; Demir, Cemil; Kulcanay Şahin, İlknur; Teksoy, Özgün; Ayhancı, Adnan
This study aims to examine cyclophosphamide (CP) exsposure associated toxicity on rat livers and the likely defensive effects of boric acid (BA). The rats used in this study were divided into four groups: control group, CP group, BA group, and BA + CP group. The present study was carried out using routine histological H&E stain, immunohistochemical stain caspase-3 as apoptotic marker, serum biochemical analysis for liver function markers (alanine transaminase (ALT), aspartate transaminase (AST) and alkalen phosphatase (ALP)), oxidative stress markers (total oxidant status (TOS), oxidative stress index (OSI) and total antioxidant capacity marker (TAC)). In the CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 increased whereas TAC levels decreased compared with the control group. In the BA + CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 decreased whereas TAC levels increased compared with the CP group. The histopathological evaluation of light microscope images and immunohistochemical caspase-3 activity in the BA + CP group were found to be decrease compared with those in the CP group. In conclusion, BA was successful in defending the liver against apoptosis and histopathological changes that are attributable to CP. © 2019 Elsevier GmbH
Citation - WoS: 4
Citation - Scopus: 3
A Histopathological, Immunohistochemical and Biochemical Investigation on the in Vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum Triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity
(Inst Tecnologia Parana, 2019) Sahinturk, Varol; Ayhanci, Adnan; Yildiz, Songul Cetik; Keskin, Cumali
The aim of this study was to investigate in vitro antioxidant properties and in vivo protective effects of the methanol extract of the Hypericum triquetrifolium Turra (HT) seed against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats induced by cyclophosphamide (CP). In order to investigate in vivo protective effects of the HT extract on rat tissues, the rats were divided into nine groups. The toxic effects of CP and the protective effects of HT extract on nucleated cells that are produced by bone marrow, serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined for histopathological changes and apoptosis by Bcl-2, Bax and caspase-3 immunohistochemistry. The results of this study show that HT seed methanol extract has high total phenolic content (179.52 mu g GAE/mg) and antioxidant activity (87.48% in 500 mu g/mL concentration). CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity in the rats. Whereas, the groups of rats that were injected with different concentrations of HT (25, 50 and 100 mg/kg) and CP (150 mg/kg) showed significant protective effects on bone marrow nucleated cells and important decreases on serum ALT, ALP, LDH and OSI levels were observed when compared with the CP injected group.
Citation - WoS: 14
Citation - Scopus: 16
In situ atom trapping of Bi on W-coated slotted quartz tube flame atomic absorption spectrometry and interference studies
(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Kilinc, Ersin; Bakirdere, Sezgin; Aydin, Firat; Ataman, O. Yavuz
Analytical performances of metal coated slotted quartz tube flame atomic absorption spectrometry (SQT-FAAS) and slotted quartz tube in situ atom trapping flame atomic absorption spectrometry (SQT-AT-FAAS) systems were evaluated for determination of Bi. Non-volatile elements such as Mo, Zr, W and Ta were tried as coating materials. It was observed that W-coated SQT gave the best sensitivity for the determination of Bi for SQT-FAAS and SQT-AT-FMS. The parameters for W-coated SQT-FAAS and W-coated SQT-AT-FAAS were optimized. Sensitivity of FAAS for Bi was improved as 4.0 fold by W-coated SQT-FAAS while 613 fold enhancement in sensitivity was achieved by W-coated SQT-AT-FAAS using 5.0 min trapping with respect to conventional FAAS. MIBK was selected as organic solvent for the re-atomization of Bi from the trapping surface. Limit of detection values for W-coated SQT-FAAS and W-coated SQT-AT-FAAS was obtained as 0.14 mu g mL(-1) and 0.51 ng mL(-1), respectively. Linear calibration plot was obtained in the range of 2.5-25.0 ng mL(-1) for W-coated SQT-AT-FAAS. Accuracy of the W-coated SQT-AT-FAAS system was checked by analyzing a standard reference material, NIST 1643e. (C) 2013 Elsevier B.V. All rights reserved.
Citation - WoS: 17
Citation - Scopus: 14
In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes
(Iranian Journal of Pharmaceutical Research, 2017) Keskin, C.; Keskin, Cumali; Aktepe, N.; Aktepe, Necmettin; Yükselten, Y.; Asuman,; Boğa, M.; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 09.01. Department of Nursing / Hemşirelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.
Citation - WoS: 11
Citation - Scopus: 7
Investigation into the protective effects of Hypericum Triquetrifolium Turra seed against cyclophosphamide-induced testicular injury in Sprague Dawley rats
(Taylor & Francis Online, 2022) Can, Senanur; Çetik Yıldız, Songül; Keskin, Cumali; Şahintürk, Varol; Cengiz, Mustafa; Appak Başköy, Sıla; Ayhanci, Adnan; Akıncı, Gülsema
For centuries, Turkey has been a significant location here around 80 species of Hypericum with differing names widely occur, which is also known as Turkish folk medicine in treating some bacterial diseases as well as stomach and intestine inflammation. Recent studies have reported this herb family to contain numbers of bioactive compound contents. The study aims to investigate the protective effects of Hypericum triquetrifolium Turra (HT) upon oxidative stress and apoptosis in a rat model in which testes injury was induced by CP. The testicular injury was caused using CP (150 mg/kg). The rats were treated with a single dose (100 mg/kg) of methanol extract of HT to investigate various biochemical markers in the serum and plasma of blood samples apart from assessing the prognosis of CP-induced testicular damage. Added to that, histological analyses were performed to identify possible structural changes and apoptotic indicators, like Bax, Caspase-3, and Bcl-2. In CP Group, there was a rise in the levels of total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), Caspase-3, and Bax while superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), Bcl-2, and total antioxidant capacity (TAC) all decreased. Also, our histological analysis showed damaged testes. On the other hand, neither biochemical nor histological analysis showed testicular damage in HT Alone Group. In CP + HT Group, a significant number of the negatives changes due to CP were observed to have improved remarkably following an HT treatment. This study results suggest that HT could help improve CP-induced testicular injury thanks to its anti-oxidative and anti-apoptotic properties.
Citation - WoS: 104
Citation - Scopus: 115
Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract
(FRONTIERS, 2022) Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Hatipoğlu, Abdulkerim
Using biological materials to synthesize metallic nanoparticles has become a frequently preferred method by researchers. This synthesis method is both fast and inexpensive. In this study, an aqueous extract obtained from chickpea (Cicer arietinum L.) (CA) leaves was used in order to synthesize silver nanoparticles (AgNPs). For specification of the synthesized AgNPs, UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron dispersive X-ray (EDX), and zeta potential (ZP) analyses data were used. Biologically synthesized AgNPs demonstrated a maximum surface plasmon resonance of 417.47 nm after 3 h. With the powder XRD model, the mean crystallite dimension of nanoparticles was determined as 12.17 mm with a cubic structure. According to the TEM results, the dimensions of the obtained silver nanoparticles were found to be 6.11–9.66 nm. The ZP of the electric charge on the surface of AgNPs was measured as −19.6 mV. The inhibition effect of AgNPs on food pathogen strains and yeast was determined with the minimum inhibition concentration (MIC) method. AgNPs demonstrated highly effective inhibition at low concentrations especially against the growth of B. subtilis (0.0625) and S. aureus (0.125) strains. The cytotoxic effects of silver nanoparticles on cancerous cell lines (CaCo-2, U118, Sk-ov-3) and healthy cell lines (HDF) were revealed. Despite the increase of AgNPs used against cancerous and healthy cell lines, no significant decrease in the percentage of viability was detected. Copyright © 2022 Baran, Fırat Baran, Keskin, Hatipoğlu, Yavuz, İrtegün Kandemir, Adican, Khalilov, Mammadova, Ahmadian, Rosić, Selakovic and Eftekhari.
Citation - WoS: 4
Citation - Scopus: 4
Investigation of serum ischemic-modified albumin, galectin-3, paraoxonase-1, and myeloperoxidase activity levels in patients with acute brucellosis
(Taylor & Francis, 2023) Dündar, Ahmet
Objectives: Infection remains current as an important discussion topic in the etiological factors of atherosclerosis. Ischemic-modified albumin (IMA), galectin-3 (gal-3), paraoxonase-1 (PON-1), and myeloperoxidase (MPO) are biomolecules that play an important role in the pathogenesis of atherosclerosis. Our aim is to investigate serum IMA, gal-3, PON-1, and MPO activity in acute brucellosis infection. Materials and methods: Forty patients with acute brucellosis and 40 healthy individuals were included in the study. Serum IMA, gal-3, PON-1, and MPO activity were analyzed by the ELISA method. Results: In acute brucellosis infection, serum gal-3, IMA, and MPO activities were found to be significantly increased compared to the control group, and PON-1 activity was found to be significantly decreased compared to the control group (p < 0.001). There was a positive correlation between serum IMA, and MPO activity (r = 0.707 p = 0.000) and a negative correlation (r = -0.943, p = 0.000) between PON-1 activity. There was a positive correlation between serum gal-3 and MPO activity (r = 0.683, p = 0.000) and IMA level (r = 0.927, p = 0.000) and a negative correlation between PON-1 activity (r = -0.951, p = 0.000).Conclusion, it was found that serum gal-3, IMA levels and MPO activity increased, while PON-1 activity decreased. These results showed that the oxidant-anti-oxidant balance is impaired in acute brucellosis infection. In addition, these results indicate that brucella infection may be increase the risk of atherosclerosis. Further studies are needed to support our findings.
Citation - WoS: 24
Citation - Scopus: 25
Investigation of the effectiveness of waste nut shell–based hydrochars in water treatment: a model study for the adsorption of methylene blue
(SpringerLink, 2022) Teğin, İbrahim; Demirel, Mehmet Ferit; Alacabey, İhsan; Yabalak, Erdal
Thousands of tons of walnut and pistachio green outer shells emerge as waste sources. Recycling such wastes in environmental applications is of great importance. In this study, the efciency of waste walnut and pistachio shell–based hydrochars in removing methylene blue (MB), which represents hazardous chemicals, from the water was investigated. Outer green pistachio shell–based hydrochar (PH) and outer green walnut shell–based hydrochar (WH) were characterized by FT-IR, SEM, EDX, TG–DTA, and BET analyses. The adsorption of MB was carried out at diferent concentrations and temperatures using WH and PH, and the adsorption parameters of Langmuir and Freundlich were investigated. The R2 values of PH were calculated as 0.9963, 0.9974, and 0.9950 and of WH adsorbent were calculated as 0.9759, 0.9939, and 0.9981 for the MB adsorption at 298 K, 313 K, and 323 K, respectively. The separation factor (RL) values for WH and PH were calculated as 0.1650≥RL≥0.103, 0.1108≥RL≥0.0177, respectively. Both adsorbents ft the Langmuir model. The ΔH° values of the WH and PH adsorbents were found to be 37.0940 and 22.2493, respectively. Positive ΔH° values indicated that the adsorption was endothermic. The negative ΔG° values of both adsorbents indicated a spontaneous adsorption process. It was shown that waste nut shell–based hydrochars can be used efectively in water treatment.
Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital
(2011) Demir Cemil; Aslantas, Ozkan; Duran, Nızamı; Ocak, S; Özer, Burcın
Aim: The aim of this study was to investigate the presence of genes encoding staphylococcal enterotoxins (SEs), exfoliative toxins (ETAs, ETBs), and toxic shock syndrome toxin-1 (TSST-1) by polymerase chain reaction (PCR) in Staphylococcus aureus strains isolated from various clinical samples from the Mustafa Kemal University Hospital. In addition, PCR-based restriction fragment length polymorphism (RFLP) analysis of the coa gene was employed to genotype the isolates. Materials and methods: A total of 120 S. aureus strains isolated from various clinical samples (blood, wounds, urine, conjuctival swabs, and tracheal aspirate) over a 1 year period, 2007-2008, were used in this study. Results: Almost 65.8% of the isolates possessed at least one toxin gene. The genes most frequently found were seg-sei (40.8%), followed by sea (30%) and eta (19.2%). Overall, 35 toxin genotypes were observed, among which the genotypes seg-sei, sea-seg-sei, and sea-see predominated at the rate of 8.3%, 5.8%, and 5%, respectively. Four coagulase genotype patterns were observed, with molecular sizes ranging from 570 to 970 bp. Coo-based RFLP analysis revealed 7 different patterns using Conclusion: Our results have revealed that toxin genes were very prevalent among S. aureus isolates, and the toxigenic isolates were independent of the genotypes obtained by PCR-RFLP of the coa gene (P > 0.05).
Citation - WoS: 9
Citation - Scopus: 11
Isolation and Molecular Characterization of Methicillin-Resistant Staphylococci from Horses, Personnel and Environmental Sites at an Equine Hospital in Turkey
(JAPAN SOC VET SCI, 2012) Aslantas, Ozkan; Turkyilmaz, Suheyla; Yilmaz, Mehmet Ali; Erdem, Zeynep; Demir, Cemil
The present study was carried out to assess the frequency of methicillin-resistant staphylococci (MRS) among racehorses (n=209) and veterinary personnel (n=13) as well as environmental surfaces (n=14) at an equine hospital in Adana, Turkey. In addition, species distribution, antimicrobial susceptibility, resistance genes, staphylococcal chromosomal cassette mec (SCCmec) type and clonality of these isolates were also investigated. MRS were identified by 16S rRNA sequencing, and typed by pulsed-field gel electrophoresis (PFGE). As a result, MRS was isolated in horses (48.3%), clinic staff (92.3%) and environmental samples (71.4%). Of the 123 MRS isolates, 118 isolates were identified as Staphylococcus lentus, and the remaining ones were found to be S. sciuri (n=3), S. intermedius (n=1) and S. fleuretti (n=1). All isolates were found to be susceptible against vancomycin, quinupristin-dalfopristin and rifampicin. Additionally, single or various combinations of resistance genes were detected among MRS isolates. SCCmec type II was identified in all isolates. Similar PFGE patterns were observed among MRS isolated from horses, humans, and environmental samples. Since MRS were concurrently isolated from horses and humans it is suggested that cross-transmission of MRS between horses and humans might occur. However, it cannot be ruled out that transmission is human to animal or animal to human.
Citation - WoS: 2
Citation - Scopus: 4
Nematode Parasites of Rockfish (Sebastes spp.) and Cod (Gadus spp.) from Waters near Kodiak Island Alaska, USA
(MDPI, 2021) Oğuz, Mehmet Cemal; Campbell, Andrea McRae; Bennett, Samuel P.; Belk, Mark C.
Distribution and abundance of common parasitic nematodes in marine fishes is not well documented in many geographic regions. Understanding the influence of large-scale environmental changes on infection rates of fish by nematodes requires quantitative assessments of parasite abundance for multiple host species. We collected samples of two species of cod and eight species of rockfish (total of 232 specimens) from waters near Kodiak Island, Alaska, USA during Spring and Summer of 2015, and dissected and recorded all internal nematode parasites. We quantified the prevalence and intensity of nematode parasites in the ten host species, and tested for differences in prevalence among host species. We found three species of nematode: Anisakis simplex, sensu lato (Van Thiel), Pseudoterranova decipiens, sensu lato (Krabbe), and Hysterothylacium sp. (Ward and Magath). Eighty-two percent of the examined fish were infected with at least one parasitic nematode. The overall prevalence of P. decipiens, A. simplex, and Hysterothylacium sp. was 56%, 62%, and 2%, respectively. Anisakis simplex and P. decipiens were abundant and present in all ten species of host fish examined, whereas Hysterothylacium sp. was rare and found in only five of the host fish species. Prevalence and mean intensity of P. decipiens and A. simplex varied across the ten host species, and the number of parasites varied substantially among individual hosts within host species. The mean intensity of P. terranova and A. simplex in our study was substantially higher than the mean intensity for these same species from multiple other locations in a recent meta-analysis. This study provides a baseline of nematode parasite abundance in long-lived fish in waters near Kodiak Island, AK, and fills an important gap in our quantitative understanding of patterns of occurrence and abundance of these common and widespread parasites of marine fish.

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