PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/3597

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A Rare Cause of Dysphagia: Giant Lipoma in Parapharyngeal Space
(Lippincott Williams and Wilkins, 2020) Aslan, Mehmet; Ozer Ozturk, Ebru; Dogukan, Fatih Mert
Background: Parapharyngeal area is one of the most complex areas of head and neck anatomy. Tumors of the parapharyngeal region are very rare among head and neck tumors. Lipomas also constitute a tiny proportion of parapharyngeal tumors so parapharyngeal area lipomas are very rare in the literature. Due to anatomical location, these tumors treatment is challenging. Clinical Report: A 20-year-old male patient admitted to our department with complaints of swallowing difficulty and a mass on the right side of the neck. Magnetic resonance imaging was performed, and it was reported as a 6 4.5 cm lipoma. He underwent excision of parapharyngeal area lipoma by a transoral approach. Conclusion: Parapharyngeal lipomas are very rare, and difficult to diagnose before they turn out symptomatic. The primary treatment approach in tumors of the parapharyngeal area is surgery and the surgical approach should be chosen according to the anatomical location of the lesion. © 2021 Elsevier B.V., All rights reserved.
Citation - WoS: 17
Citation - Scopus: 15
Alpha-lipoic acid may ameliorate testicular damage by targeting dox-induced altered antioxidant parameters, mitofusin-2 and apoptotic gene expression
(Andrologia, 2021) Güzel, Elif Erdem; Kaya Tektemur, Nalan; Tektemur, Ahmet; 09.02. Department of Midwifery/ Ebelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In the study, the ameliorating effects of alfa lipoic acid (ALA) against doxorubicin-induced testicular apoptosis, oxidative stress and disrupted mitochondrial fusion were investigated in male rats. Rats were divided into four groups as control, doxorubicin (DOX), DOX + ALA and ALA. A single dose of 15 mg/kg DOX was administered i.p to the DOX and DOX + ALA groups. 50 mg/kg ALA was given to the DOX + ALA and ALA groups by oral gavage every other day. After 28 days, rat testes and serum samples were collected and analysed. Administration of DOX alone caused a decrease in body and relative testicular weights, seminiferous tubule diameter and germinal epithelium thickness, Johnsen's score and serum testosterone levels. DOX treatment led to severe testicular damage such as tubular degeneration, and atrophic tubules. Also, the activities of superoxide dismutase and glutathione peroxidase were reduced, while the level of malondialdehyde was increased in the testis. The mRNA levels of apoptotic-related genes (CASP3, TP53, BAX, BCL2) and apoptotic index were increased, while mitofusin-2 decreased. DOX caused an increase in CASP3 and a decrease in mitofusin-2 immunoreactivities. Treatment with ALA markedly improved all of DOX-induced biochemical, histochemical and molecular alterations in rat testis. Consequently, ALA has a therapeutic role in ameliorating DOX-induced testicular damage in rats.
Citation - WoS: 6
Citation - Scopus: 6
Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium
(Wiley Online Library, 2022) Aktepe, Necmettin; Baran, Ayşe; Atalar, Mehmet Nuri; Baran, Mehmet Fırat; Keskin, Cumali; Taşkin, Abdullah; Yavuz, Ömer; Demirtaş, İbrahim; Oğuz, Ercan; Jahan, Israt; 09.01. Department of Nursing / Hemşirelik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography–electrospray ionization– tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50mg/ml, 5.0 μg/ml). A 500μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.
Citation - WoS: 1
Citation - Scopus: 1
Anatolian Medicinal Plants as Potential Antiviral Agents: Bridging Traditional Knowledge and Modern Science in the Fight Against Covid-19 and Related Viral Infections
(Tubitak Scientific & Technological Research Council Turkey, 2024) Tilkat, Engin; Jahan, Israt; Hoser, Ayse; Kaplan, Alevcan; Ozdemir, Oguzhan; Onay, Ahmet; 21.01. Department of Health and Nursing Services / Sağlık ve Bakım Hizmetleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the cause of the coronavirus 2019 (COVID-19), commonly known as the coronavirus pandemic. Since December 2020, COVID-19 vaccines have been extensively administered in numerous countries. In addition to new antiviral medications, the treatment regimen encompasses symptom management. Despite sustained research efforts, the outbreak remains uncontrolled, with affected patients still lacking proper treatment. This review is a valuable asset for researchers and practitioners aiming to delve into the yet unexplored potential of Anatolian flora in the fight against COVID-19 and other viral infections. Numerous medicinal plants in Anatolia, such as thyme, sage, cannabis, oregano, licorice root, and Origanum sp., contain bioactive compounds with proven antiviral properties that have been used in the region for centuries. The rich legacy of traditional Anatolian medicine (TAM), has significantly influenced modern medicine; thus, the profusion of medicinal plants native to Anatolia holds promise for antiviral drug development, making this review essential for researchers and practitioners.
Citation - WoS: 10
Citation - Scopus: 13
The antioxidant and anti-apoptotic potential of Pleurotus eryngii extract and its chitosan-loaded nanoparticles against doxorubicin-induced testicular toxicity in male rats
(Wiley, 2021) Erdem Güzel, Elif; Kaya Tektemur, Nalan; Tektemur, Ahmet; Acay, Hilal; Yıldırım, Ayfer; 09.02. Department of Midwifery/ Ebelik Bölümü; 09.03. Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study was conducted to evaluate the protective role of Pleurotus eryngii extract (PE) and Pleurotus eryngii extract-loaded chitosan nanoparticles (PE-CSNP) against doxorubicin (DOX)-induced testicular toxicity in rats. Male rats were divided into six groups: control (DMSO/ethanol), PE (200 mg/kg PE), PE-CSNP (30 mg/kg PECSNP), DOX (10 mg/kg DOX, a single dose, i.p), DOX+PE (10 mg/kg DOX+200 mg/ kg PE) and DOX+PE-CSNP (10 mg/kg DOX+30 mg/kg PE-CSNP). PE and PE-CSNP were administered by oral gavage every other day for 21 days. DOX-treated rats showed histopathological impairment compared with the control group. There was an increase in the apoptotic index, caspase 3 (CASP3), BCL2-associated X apoptosis regulator (BAX), dynamin-related protein 1 (DRP1) expression and total oxidative status (TOS) in the DOX group, while mitofusin-2 (MFN2), total antioxidative status (TAS) and serum testosterone levels of the DOX group reduced when compared with the other groups. PE and PE-CSNP treatments provided significant protection against DOX-induced oxidative stress by reducing TOS levels and increasing TAS levels. CASP3, BAX, apoptotic index and DRP1-MFN2 expressions were restored by PE and PE-CSNP. However, the PE-CSNP showed higher antioxidant and anti-apoptotic efficacy compared with PE. Thus, our results provide evidence that CSNP and PE could synergistically have a potent antioxidant and anti-apoptotic therapy against DOX-induced testicular damage in male rats.
Citation - WoS: 1
Citation - Scopus: 1
Association Between the Triglyceride-Glucose Index and Contrast-Induced Nephropathy in Chronic Total Occlusion Patients Undergoing Percutaneous Coronary Intervention
(Bmc, 2025) Soner, Serdar; Aktan, Adem; Kilic, Raif; Guzel, Hamdullah; Tastan, Ercan; Oksul, Metin; Guzel, Tuncay; Department of Internal Medical Sciences / Dahili Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Objective The triglyceride glucose (TyG) index is a biomarker of insulin resistance and is associated with an increased risk of cardiovascular events. Contrast-induced nephropathy (CIN) is an important complication that causes poor outcomes in patients undergoing percutaneous coronary intervention (PCI). In this study, we aimed to investigate the relationship between the TyG index and CIN and mortality in patients who underwent PCI due to chronic total coronary occlusion (CTO). Methods Two hundred eighteen individuals from three separate medical centers who underwent procedural PCI between February 2010 and April 2012 and had a CTO lesion in at least one coronary artery were recruited. According to the TyG index, patients were divided into two groups. Patients with a TyG index >= 8.65 were included in Group 1, and patients with a TyG index < 8.65 were included in Group 2. Patients were followed up for 96 months. The main outcome was the development of CIN and mortality. Results The mean age of the patients (65.8 +/- 10.94 vs. 61.68 +/- 11.4, P = 0.009), diabetes mellitus (60 [44.8%] vs. 11 [13.1%], P < 0.001), and dyslipidemia rates (52 [38.8%] vs. 21 [25%], P = 0.036) were higher in group 1. In multivariable logistic regression analysis, it was seen that age (OR = 1.04, 95% CI = 1.01-1.08, P = 0.020), chronic kidney disease (OR = 2.34, 95% CI = 1.02-5.33, P = 0.044), peripheral artery disease (OR = 5.66, 95% CI = 1.24-25.91, p = 0.026), LVEF (OR = 0.95, 95% CI = 0.92-0.99, P = 0.005), LDL cholesterol levels (OR = 1.00, 95%CI = 1.00-1.02, P = 0.024) and TyG index (OR = 2.17, 95% CI = 1.21-3.89, P = 0.009) were independent predictors of the development of CIN. Conclusion Our study demonstrates a correlation between the TyG index and the prevalence of CIN in patients with CTO undergoing PCI. Adding the TyG index to the routine clinical evaluation of patients with CTO undergoing PCI may help protect patients from the development of CIN.
Citation - WoS: 2
Citation - Scopus: 2
Association of Inflammatory and Metabolic Markers With Mortality in Patients With Postoperative Femur Fractures in the Intensive Care Unit
(Mdpi, 2025) Kilinc, Metin; Celik, Enes; Demir, Ibrahim; Aydemir, Semih; Akelma, Hakan; Department of Surgical Medical Sciences / Cerrahi Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Background and Objectives: Postoperative femur fracture in elderly patients is associated with high morbidity and mortality, especially in the intensive care unit (ICU). Various factors, including demographic and laboratory parameters, may influence mortality in this population. The aim of this study was to evaluate the association of inflammatory and metabolic markers with mortality in ICU patients with postoperative femur fractures and to identify key predictors to enhance risk stratification and improve patient outcomes. Materials and Methods: In this retrospective single-center study, we analyzed 121 patients aged over 65 years with postoperative femur fractures who were admitted to the ICU between January 2023 and January 2024. Demographic and clinical data, including comorbidities, Charlson Comorbidity Index (CCI), and Acute Physiology and Chronic Health Evaluation (APACHE II) score, were collected. Laboratory parameters such as white blood cell count (WBC), albumin, C-reactive protein (CRP), D-dimer, Pan-Immune-Inflammation Value (PIV), CRP-to-albumin ratio (CAR), neutrophil-to-lymphocyte ratio (NLR), and others were analyzed. Linear regression, logistic regression, and Receiver Operating Characteristic (ROC) analyses were performed to determine the predictive value of these markers for ICU mortality. Results: The mean age of the patients was 76.3 +/- 9.6 years, and 52.1% were female. The most common comorbidities were hypertension (67.8%) and diabetes (49.6%). ICU mortality occurred in 24 patients (20%). Significant predictors of mortality included higher CRP (>62.8 mg/L), NLR (>10.0), PIV (>450), and APACHE II scores (>23) (p < 0.001 for all). Lower albumin levels (<2.5 g/dL) were strongly associated with increased mortality (p < 0.001). ROC analysis demonstrated that the APACHE II score had the highest predictive accuracy for mortality (AUC = 0.83), followed by albumin (AUC = 0.79) and PIV (AUC = 0.76). Extended ICU stay (>10 days) was also significantly correlated with increased mortality (p < 0.001). Conclusions: This study successfully demonstrates the utility of combining traditional clinical markers, such as APACHE II score, with novel inflammatory markers, such as PIV, CAR, and NLR, in predicting mortality in ICU patients following femur fracture surgery. The integration of emerging biomarkers with well-established scoring systems offers enhanced predictive accuracy and provides valuable insights into patient management.
Citation - WoS: 41
Citation - Scopus: 40
Association with Leptin Gene c.-2548 G > A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients
(HUMANA PRESS INC, 2013) Say Şahin, Deniz; Tümer, Cemil; Demir, Cemil; Çelik, M. Murat; Çelik, Mustafa; Uçar, Edip; Güneşaçar, Ramazan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G > A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G > A polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G > A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p < 0.001) and body mass index (p < 0.0001). All these consequences showed that LEP -2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population.
Citation - WoS: 1
Citation - Scopus: 1
Can Quercetin Reduce Arsenic Induced Toxicity in Mouse Balb/C 3t3 Fibroblast Cells ? A Study Involving in Vitro, Molecular Docking, and Adme Predictions
(Bmc, 2025) Unsal, Velid; Keskin, Cumali; Oner, Erkan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 09.03. Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
This study aimed to investigate the protective effect of quercetin against arsenic-induced oxidative damage, inflammation, and apoptosis in mouse BALB/c 3T3 fibroblast cells (NIH-3T3). Arsenic at different concentrations of 0.05 mu M (low), 0.5 mu M (medium), 10 mu M (high) doses were used to induce toxicity, while 120 mu m quercetin was used for treatment. MTT and LDH analyses were performed to determine the effect of arsenic and quercetin on cell viability, while oxidative stress markers and antioxidant enzyme activities were measured by spectrophotometric method. TNF-alpha and IL-1 beta levels were measured by the ELISA method, Autodock programs were used for molecular docking studies. In addition, computer-based analyses of quercetin and succimer molecules were performed using SwissADME web tools. TNF-alpha (PDB ID: 2AZ5), IL-1 beta (PDB ID: 1ITB), Caspase3 (PDB ID: 2XYG), Bax (PDB ID: 4S0O), SOD (PDB ID:1CBJ), GSH-Px (PDB ID: 1GP1) and Bcl-2 (PDB ID: 1G5M) crystal structures were obtained from the Protein Data Bank. Bax and Bcl-2 levels of apoptotic genes and mRNA expression levels of Caspase-3 activity were measured using the QRT-PCR technique. TUNEL staining was performed to determine DNA fragmentations, while DAPI staining was done to visualise nuclear modifications. Quercetin has been found to significantly reduce oxidative stress, inflammation, and apoptosis in cells and exert anti-apoptotic effects. Molecular docking studies revealed quercetin shows good binding affinity with molecules with SOD, GSH-Px, Bax, Bcl-2, Caspase-3, TNF-alpha and IL-1 beta structures, and has been observed to bind with Bax and Bcl-2 with molecular docking scores of -7.5 and - 7.7 kcal/mol, respectively. These findings are supported by results showing that quercetin is effective in anti-apoptotic and anti-inflammatory processes in arsenic-induced cells under in vitro conditions. In addition, when ADME values are examined, it can be considered that quercetin is a useful and effective candidate compound in reducing arsenic toxicity, considering its higher synthetic accessibility score, better pharmacokinetic properties, and good biological transition and interaction capacities compared to succimer.
Citation - WoS: 8
Citation - Scopus: 9
Carbamazepine-induced renal toxicity may be associated with oxidative stress and apoptosis in male rat
(Taylor & Francis Online, 2021) Erdem Güzel, Elif; Kaya Tektemur, Nalan; Tektemur, Ahmet; Etem Önalan, Ebru; 09.02. Department of Midwifery/ Ebelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Carbamazepine (CBZ) is the antiepileptic drug used in epilepsy and some psychiatric disorders. Besides its widely used, many adverse effects have been reported including hematotoxicity, hepatotoxicity, endocrine disorders, and testicular damages due to oxidative stress. However, the role of CBZ on renal toxicity is not fully known. In this study, we attempted to explain the connected mechanisms by focusing on the metabolism of CBZ-induced renal toxicity in rats. Twenty male Wistar-Albino rats were randomized into 2 groups (n = 10); control (1 mL/day distilled water, orally) and CBZ (25 mg/kg/day CBZ, orally) groups. After 60 days, TAS (total oxidant status) and TOS (total oxidant status) levels, histopathological features, some genes involved in apoptosis, 8-hydroxy-2-deoxyguanosine (8-OHdG) activity, and apoptotic cells were assessed of kidney tissue. The oxidative stress index (OSI) was measured from TAS and TOS levels. TOS levels and OSI significantly increased, while TAS levels decreased in the CBZ group relative to the control group. Histopathological observations, Caspase-3 (Casp3), Poly [ADP-ribose] polymerase-1 (PARP-1), 8-OHdG immunoreactivities, and apoptotic cells markedly raised in the CBZ group compared with the control group. Also, mRNA expression of Cytochrome c (Cytc) and CASP3 significantly increased in the CBZ group compared to the control group. In conclusion, long-term use of CBZ may promote renal damage in rats by inducing oxidative stress and apoptosis.
Cardiac Autonomic Control Reflects Sympathovagal Changes Associated With Withholding Urination
(Wiley, 2025) Alabdo, Ahmad; Oflazoglu, Basak; Kus, Mehmet Mustafa; Cakan, Pinar; Ugras, Seda; Yildiz, Sedat
IntroductionIncreased volume of the urinary bladder causes urge to urinate and constricts the sphincter by increasing the tonus of the sympathetic nervous system (SNS). Activity of the autonomic nervous system (or sympatho-vagal balance) can be assessed by heart rate variability (HRV). Current study aimed to test in healthy young male participants whether HRV technique reflects increased sympathovagal balance during bladder distention as revealed by strong urge to urinate.Materials and MethodsA total of young and apparently healthy 11 males agreed to participate to the current study. They were asked to provide 5-min continuous electrocardiogram (ECG) recordings in supine position for determination of HRV on two occasions, i.e. before and approximately for 3 1/2 hours after withholding urination. HRV parameters included heart rate (HR, per min), total power (TP, ms2), and standard deviation of RR intervals (SDNN, ms); percentage of successive RR intervals that were different >= 50 ms (pNN50), low frequency (LF) and high frequency (HF) bands and their normalized units (LFnu and HFnu, respectively) and their ratios (LF/HF). Half of the students provided samples on both occasions and those data were compared by paired t-test following log10 transformation.ResultsWithholding urination did not change HR (from 80.0 +/- 4.1 to 84.5 +/- 3.1 per min, p = 0.135) but decreased total power (from 2692 +/- 802 to 1605 +/- 357 ms2, p = 0.008), SDNN (from 50.4 +/- 5.8 to 38.7 +/- 3.8 ms, p = 0.007) and pNN50 (from 15.6 +/- 3.3 to 5.9 +/- 2.6%, p = 0.016) and increased LFnu (from 57.3 +/- 5.3 to 65.1 +/- 4.5, p = 0.029) and LF/HF ratio (from 1.77 +/- 0. 73 to 2.42 +/- 0.47, p = 0.047).ConclusionsIncreased LF/HF together with decreased SDNN and pNN50 suggest that HRV successfully reflects increased sympathetic tonus over the parasympathetic to help accommodate the urine in the bladder. Moreover, it seems that HRV can be used to assess sympathovagal changes non-invasively during voiding. Additionally, it appears that in all HRV measurements, participants should not have postponed their urination during recording.
Citation - WoS: 3
Citation - Scopus: 4
Chronic effects of maternal tobacco-smoke exposure and/or α-lipoic acid treatment on reproductive parameters in female rat offspring
(Taylor & Francis Online, 2020) Erdem Güzel, Elif; Nalan Kaya, Ahmet Tektemur, Nazife Ulker, Ahmet Yardimci, Ramazan Fazil Akkoc, Sinan Canpolat & Ibrahim Enver; 09.02. Department of Midwifery/ Ebelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Prenatal tobacco-smoke exposure negatively affects the reproductive functions of female offspring and oxidative stress plays a major role at this point. Alpha-lipoic acid (ALA), well known as a biological antioxidant, has been used as a nutritional supplement and as a therapeutic agent in the treatment of certain complications during pregnancy. We aimed to investigate the effects of maternal tobacco-smoke exposure and/or ALA administration on puberty onset, sexual behavior, gonadotrophin levels, apoptosis-related genes, apoptotic cell numbers and oxidative stress markers in the adult female rat offspring. Sprague-Dawley rats were divided into four groups; control, tobacco smoke (TS), TS+ALA and ALA groups. Animals were exposed to TS and/or ALA for 8 weeks before pregnancy and throughout pregnancy. All treatments ended with birth and later newborn female rats were selected for each experimental group. The experiment ended at postnatal day 74-77. Maternal tobacco smoke advanced the onset of puberty in the female offspring of the TS group (p < 0.05). In all treatment groups; the mean number of anogenital investigations and lordosis quality scores showed a decline, serum luteinizing hormone levels significantly increased (p < 0.05) and several histopathological changes in ovaries were observed compared to the control group. In addition, an increase in apoptotic marker levels and apoptotic cell numbers was detected in the ovaries of all treatment groups. Decreased TAS and increased TOS levels were detected in all treatment groups compared to control. These findings suggested that maternal tobacco smoke and/or ALA administration may be leading to the impaired reproductive health of female offspring. Abbreviations: ALA: alpha-lipoic acid; LH: luteinizing hormone; FSH: follicle-stimulating hormone; TAS: total antioxidant status; TOS: total oxidant status; Apaf1: apoptotic protease-activating factor 1; Casp3: caspase 3; Casp9: caspase 9; CF: cyst follicles; 4-HNE: 4-Hidroxynonenal; 8-OHdG: 8-hydroxydeoxyguanosine; TUNEL: terminal deoxynucleotidyl transferase-mediated deoxyuridine-biotin nick end labeling; ROS: reactive oxygen species; GnRHR: gonadotropin-releasing hormone receptor; HPG: hypothalamic-pituitary-gonadal; AMPK: AMP-activated protein kinase; ELISA: enzyme-linked immunosorbent assay; cDNA: complementary DNA; qPCR: quantitative real-time PCR; FC: follicular cysts; PF: primary follicle; SF: secondary follicle; GF: graafian follicle; CL: corpus luteum; DF: degenerated follicle; AF: atretic follicle.
Citation - WoS: 16
Citation - Scopus: 17
A Class Activation Map-Based Interpretable Transfer Learning Model for Automated Detection of ADHD from fMRI Data
(Sage Journals, 2022) Uyulan, Caglar; Erguzel, Turker Tekin; Türk, Ömer; Farhad, Shams; Metin, Bariş; Tarhan, Nevzat; 08.01. Department of Computer Engineering / Bilgisayar Mühendisliği Bölümü; 08. Faculty of Engineering and Architecture / Mühendislik Mimarlık Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Automatic detection of Attention Deficit Hyperactivity Disorder (ADHD) based on the functional Magnetic Resonance Imaging (fMRI) through Deep Learning (DL) is becoming a quite useful methodology due to the curse of-dimensionality problem of the data is solved. Also, this method proposes an invasive and robust solution to the variances in data acquisition and class distribution imbalances. In this paper, a transfer learning approach, specifically ResNet-50 type pre-trained 2D-Convolutional Neural Network (CNN) was used to automatically classify ADHD and healthy children. The results demonstrated that ResNet-50 architecture with 10-k cross-validation (CV) achieves an overall classification accuracy of 93.45%. The interpretation of the results was done via the Class Activation Map (CAM) analysis which showed that children with ADHD differed from controls in a wide range of brain areas including frontal, parietal and temporal lobes.
Comparison of Orexigenic and Anorexigenic Neuropeptide Levels in Hyperemesis Gravidarum Patients With Normal Pregnant Women: a Prospective Cohort Study
(Lippincott Williams & Wilkins, 2024) Yilmaz, Mehmet; Aksin, Serif; Balsak, Deniz; Aboalhasan, Yasmin; Batmaz, Ibrahim; Department of Surgical Medical Sciences / Cerrahi Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Background: The aim of this study was to determine whether orexigenic neuropeptides, orexin and galanin, and anorexigenic neuropeptides, alpha-melanocyte-stimulating hormone (alpha-MSH) and cocaine- and amphetamine-regulated transcript (CART), are implicated in hyperemesis gravidarum (HG). Methods: Fifty pregnant women who had been diagnosed with HG between April 2022 and February 2023 at the Siirt University Faculty of Medicine Training and Research Hospital (tertiary center) were recruited for this study. An equal number of pregnant women without an HG diagnosis were included in the study as the control group. Participants' age, pregnancy history, medical history, thyroid function test results, complete blood count results, and electrolyte levels were recorded, and their orexin, galanin, alpha-MSH, and CART serum levels were analyzed using an enzyme-linked immunosorbent assay. Results: No statistically significant differences in orexigenic neuropeptides (orexin and galanin) were observed between the HG and control groups. A statistical difference was found between an anorexigenic neuropeptide (alpha-MSH) and the control group (P = .012). Based on a receiver operating characteristic analysis, the alpha-MSH parameter was statistically significant for distinguishing between participants with an HG diagnosis and those without, with a sensitivity of 63.6%, specificity of 65.9%, and cutoff value of 11769.3 pg/mL (P = .012, area under curve: 0.655). Based on the severity classification of ketonuria (ketonuria levels of +1 or +2 were classified as mild, whereas levels of +3 or +4 were classified as moderate to severe), the anorexigenic CART neuropeptide was found to be a statistically significant diagnostic indicator of severe ketonuria (P = .020). Conclusion: alpha-MSH and CART levels were found to be related in HG patients and in HG patients with severe ketonuria.
Citation - WoS: 1
Citation - Scopus: 1
Comparison of Pde-5 Inhibitors Used in Erectile Dysfunction With Some Candidate Molecules: a Study Involving Molecular Docking, Admet, Dft, Biological Target, and Activity
(Bmc, 2025) Sagir, Suleyman; Unsal, Velid; Oner, Erkan; Yildiz, Resit; Mert, Basak Dogru; 09.03. Department of Nutrition and Dietetics/ Beslenme ve Diyetetik Bölümü; Department of Surgical Medical Sciences / Cerrahi Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Erectile dysfunction (ED) is a urological condition defined as the inability of a man to achieve or maintain an erection. This condition negatively affects his sexual performance and the performance of his partner. Phosphodiesterase type 5 (PDE5) inhibitors are commonly used to treat ED. Arginase II plays an important role in regulating L-arginine to NO synthase in the smooth muscle of the human corpus cavernosum of the penis. NO is a molecule essential for regulating a variety of functions, including arterial blood pressure, penile erection, and energy balance. Substances such as vardenafil, alprostadil, papaverine, and resveratrol increase NO production, thereby supporting sexual function and vascular health. Additionally, NO donors such as L-arginine, L-citrulline, and alpha-lipoic acid provide effective alternatives when used in combination with PDE5 inhibitors. Medications used in the treatment of ED include vardenafil, alprostadil, and papaverine. In addition, although molecules such as L-arginine, citrulline, resveratrol, alpha-lipoic acid, and rutin are thought to play a role in ED, their pharmacological and molecular effects have not been sufficiently elucidated. The aim of this study was to investigate the effects of these molecules in the treatment of ED by computer-based calculations, to obtain new information about them and to inspire new treatment strategies for ED. The physicochemical, molecular and pharmacokinetic properties of the compounds were determined by SwissADME software, and ADMET (absorption, distribution, metabolism, excretion and toxicity) data were determined by ADMETlab 3.0 software. Biological target and activity data were obtained by MolPredictX and PASS Online software. While the Gaussian 09 program was used for DFT calculations, PyMOL, AutodockTools 4.2.6, AutoDock Vina, and Biovia Discovery programs were used for molecular docking studies. It was found that L-arginine, citrulline, resveratrol and alpha-lipoic acid were well absorbed from the intestine, while rutin showed limited absorption. When their metabolic risks were evaluated, L-arginine and citrulline were found to have lower toxicity. Molecular docking results of rutin and resveratrol were remarkable. The electronic properties of the compounds were explained by DFT calculations. L-arginine and citrulline were found to have low toxicity and positive therapeutic effects. L-arginine and citrulline stand out as promising candidates for future research. Although resveratrol data are promising, unfortunately their potential toxicity and metabolic interactions require further investigation. It is important to learn more about these compounds or conduct research to improve their therapeutic efficacy. Although computer-based calculations play an important role in toxicity predictions, drug interactions, pharmacokinetics and toxicity properties should be carefully evaluated.
Confounding Factors in the Tear Break-Up Time Test. Influence of Sequential Measurements
(Taylor & Francis Ltd, 2025) Acet, Yakup; Dag, Yasar; Department of Surgical Medical Sciences / Cerrahi Tıp Bilimleri Bölümü; 10. Faculty of Medicine / Tıp Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Clinical relevance The tear break-up time (BUT) test is important in diagnosing and monitoring tear film stability, and understanding potential changes in tear film dynamics during repeated measurements could improve the accuracy and reliability of the test in clinical practice. Background The study aimed to examine the changes in BUT value caused by subsequent measurements made in BUT test. Methods A total of 120 female participants were divided into groups (30 individuals each). Participants were divided into four groups based on the interval between two non-invasive tear break-up time (NI-BUT) tests: Group 1 (1-minute interval), Group 5 (5-minute interval), Group 10 (10-minute interval), and Group 15 (15-minute interval). The first NI-BUT test was called Initial NI-BUT (NI-BUT(I)), and the second test was called Subsequent NI-BUT (NI-BUT(S)). The first BUTvalue (BUT1st), the second BUT value (BUT2nd), the third BUT value (BUT3rd), the fourth BUT value (BUT4th), the fifth BUT value (BUT5th), and the average value of the first three BUTs (BUTA3) were compared between the NI-BUT(1) and NI-BUT(S) tests. Results Participant ages ranged from 18 to 36 years, with a mean of 25.1 +/- 3.2 years. The mean values of BUT1st, BUT2(nd), BUT3(rd), BUT4(th), BUT5(th), and BUTA3 in the NI-BUT(I) test were statistically significantly shorter than those in the NI-BUT(S) test for all groups (p < 0.05). The changes in the parameters were statistically significantly greater in Group 1 compared to the other groups (p < 0.05). Conclusions Subsequent measurements cause increased BUT values. The longer the interval between two measurements, the smaller the differences between the subsequent measurements. When performing the BUT test, a single measurement should be preferred if possible. If the clinician finds the results of a single measurement unsatisfactory and wishes to consider the average of multiple measurements, the interval between tests should be longer than 15 minutes.
Citation - WoS: 58
Citation - Scopus: 64
Cyclophosphamide induced oxidative stress, lipid per oxidation, apoptosis and histopathological changes in rats: Protective role of boron
(Elsevier, 2020) Cengiz, Mustafa; Şahintürk, Varol; Cetik Yildiz, Songul; Kurcanay Şahin, İlknur; Bilici, Namık; Onur Yaman, Suzan; Altuner, Yılmaz; Appak-Baskoy, Sıla; Ayhanci, Adnan; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Background Cyclophosphamide (CP) is an alkylating chemotherapeutic drug used in the treatment of many types of cancer. However, as with other chemotherapeutic drugs, the use of CP is limited by the damage to healthy tissues such as testes, bladder and liver as well as cancerous tissue. Boron (B) is a trace element with many biological properties such as antioxidant, anti-apoptotic and anti-lipid per oxidation. Methods This current study aims to determine protective effects of B on CP induced testicular toxicity. The rats were divided into 4 groups (control, CP, B and B plus CP groups). The testes of experimental animals were taken for histological, apoptotic markers and biochemical analysis. Results The damage to some seminifer tubules, loss of typical appearance, thinning of seminifer epithelium and relative enlargement of the tubule lumen were watched in testis of the group that administrated CP. Moreover, Bcl-2, TAC and GSH levels decreased while TOC, OSI, MDA, Bax and Caspase-3 levels increased. On the other hand, pretreatment limited to B in the B plus CP group, testicular tissue improved. In addition, Bcl-2, GSH, TAC levels increased, Bax, MDA, TOC, OSI and caspase-3 levels decreased. Conclusion B significantly reduced testicular lipid per-oxidation and strengthened antioxidant defenses. Our results showed that pre-treatment B can protect rat testis against CP-induced testicular damage owing to its anti-lipid per oxidation, anti-oxidant and anti-apoptotic properties.
Citation - WoS: 10
Citation - Scopus: 12
Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II)
(Analytical Biochemistry, 2021) Kılınç, Ersin; Ozdemir, Sadin; Şen, Fatih; Soylak, Mustafa; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min−1 of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL−1, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
Does the Presence of Chronic Lymphocytic Thyroiditis Affect Diagnostic Value of Fine Needle Aspiration Biopsy in Bethesda Category III Nodules?
(Dr. Mladen Stojanovic University Hospital, 2023) Pedük, Şevki; Koçer, Belma
This study aimed to determine the relationship between the presence of Hashimo-to’s thyroiditis (HT) and malignancy rates with prognostic factors in thyroid nodules diagnosed as Bethesda category III, and to examine the effect of HT on diagnostic value of fine-needle aspiration biopsy (FNAB). Demographic information, preoperative examination, and final pathological evaluation of patients with Bethesda category III (AUS-FLUS) nodules who had been operated on in our department over the last 6 years were analyzed. Statistical analyses were performed using the Student’s t-test, Mann-Whitney U test and χ2-test and logistic regression analysis using SPSS version 22 software. The malignancy rate on final pathology of 159 patients was 24.5%. Malignancy rates were found to be higher in patients with HT coexistence (30.7% vs. 21.5%, p=0.20). Poor prognostic factors such as multifocality, number of metastatic lymph nodes (p=0.04), and extrathy-roidal extension were more common in patients with cancer in the pathology specimen who were in the non-HT group. It cannot be said that HT decreases diagnostic value of FNAB in lesions diagnosed with AUS-FLUS. The lower incidence of poor prognostic factors in the HT group may be attributed to cytotoxic cell dominance in tumor immunity. © 2024 Elsevier B.V., All rights reserved.
Citation - WoS: 5
Citation - Scopus: 7
The effect of acupressure on postoperative pain of lumbar disc hernia: A quasi-experimental study
(ELSEVIER SCI LTD, 2018) Tanrıverdi, Seher; Sarıtaş, Serdar; 09.06. Department of Social Services/ Sosyal Hizmet Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
Lumbar disc herniation (LDH) is a disease characterized by lumbar and leg pain that occurs as a result of compression of the lumbar spinal root by the degenerated disc. The results of the study showed that 80% of humans experienced lumbar pain in any period of their lives [[1], [2], [3]]. Comprising an important part of lumbar pain; lumbar disc herniation (LDH) has an prevalence of approximately 15–56% per year [4,5]. One of the treatment methods preferred for patients diagnosed with lumbar disc herniation is surgery. Patients, who undergo surgery, experience intense pain in the early postoperative period [6]. It is known that medications are commonly used in pain control. Analgesic treatment used for pain control is the most preferred treatment method for pain relief as it has a rapid action and is administered easily [7,8]. Damages in both individual and national economy due to preferring carelessly and frequently analgesics affect negatively some physiological functionings and have negative effects such as development of addiction with the increase of dosages every time especially in cases where narcotics are preferred [9]. Thus, it is indicated that the administration of non-pharmaceutical methods by nurses to relieve the postoperative pain in patients in addition to medications increases the action of medications [8]. It is anticipated that there are numerous non-pharmaceutical methods which are efficient in pain management, such as distraction, relaxation, therapeutic touch, transcutaneous nerve stimulation (TENS), informing, hypnotherapy, acupuncture, massage, aromatherapy, music and meditation, yoga, attention-grabbing, imagination, cognitive-behavioral techniques. But, it is known that nurses do not use these methods adequately to relieve pain [10]. It may be asserted that non-pharmaceutical treatments are among independent nursing roles of professional nurses who have theoretical knowledge and problem solving ability. Nurses are required to take a role in non-pharmaceutical interventions in order to meet the health needs of individuals, families and society [11]. One of the non-pharmaceutical treatment methods used in pain relief is acupressure. Applied with touch pressuring on the acupuncture points using hands, fingers, thumbs or small beads according to the traditional Chinese medicine; acupressure is a non-pharmaceutical pain relief example that balances energy in the body [[12], [13], [14]]. Advantages of acupressure include low cost, learning convenience and non-invasive characteristics [14]. Besides, randomized clinical studies conducted to compare physiotherapy and acupressure in decreasing lumbar pain (LBP) have reveal that acupressure might be effective [[15], [16], [17]]. The use of acupressure in nursing practices by taking all its positive effects into consideration will contribute to the establishment of qualified nursing care standards.

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