Sağlık Hizmetleri Meslek Yüksekokulu

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Citation - WoS: 42
Citation - Scopus: 40
Association with Leptin Gene c.-2548 G > A Polymorphism, Serum Leptin Levels, and Body Mass Index in Turkish Obese Patients
(HUMANA PRESS INC, 2013) Say Şahin, Deniz; Tümer, Cemil; Demir, Cemil; Çelik, M. Murat; Çelik, Mustafa; Uçar, Edip; Güneşaçar, Ramazan
Leptin is a protein hormone which plays a critical role in the regulation of both body-weight through reducing food intake and stimulating energy expenditure. Several polymorphisms in leptin gene (LEP), which encodes for leptin, have been described. However, its association with obesity is still controversial. Therefore, in the present study, we aimed to investigate whether LEP c.-2548 G > A polymorphism was associated with serum leptin levels, lipid parameters, and body mass index in Turkish obese patients. Forty-seven obese patients and 48 healthy individuals were included in the study. Blood samples were collected for DNA extraction. LEP c.-2548 G > A polymorphism were detected using polymerase chain reaction-restriction fragment length polymorphism technique. Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques, respectively. GA or AA genotypes and A allele carrier frequencies of the c.-2548 G > A polymorphism in the LEP were higher in obese (38.3, 34.0 and 72.3 %) when compared with controls (14.6, 12.5, and 27.1 %; p = 0.011, 0.016, and 0.002, respectively). On the other hand, AA or AG genotypes were also related to increased serum leptin levels (p < 0.001) and body mass index (p < 0.0001). All these consequences showed that LEP -2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population.
Citation - Scopus: 16
Basic Red 18 and Remazol Brilliant Blue R biosorption using Russula brevipes, Agaricus augustus, Fomes fomentarius
(Water Practice and Technology, 2022) Arslantaş, Ceren; M’barek, Islem; Saleh, Mohammed; Isik, Zelal; Ozdemire, Sadin; Dündar, Abdurrahman; Dizge, Nadir
In this study, the adsorption abilities of Russula brevipes (RB), Agaricus augustus (AA), and Fomes fomentarius (FF) were evaluated in Basic Red 18 (BR18) and Remazol Brilliant Blue R (RBBR) biosorption from textile effluent. The adsorbents were characterized via Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometer (SEM-EDS), and Fourier transform infrared spectroscopy (FT/IR). Fomes fomentarius presented a low sorption capacity contrary to the two other fungi (RB and AA). RB and AA were selected as potential adsorbents for BR18 and RBBR, respectively. The maximum BR18 removal efficiencies for 10, 25, and 50 mg/L were obtained after 60 min to be 90, 88, and 86%, respectively. The RBBR adsorption efficiencies were 96.4, 96, and 90% for dye concentrations of 10, 25, and 50 mg/L. The adsorption of BR18 onto the RB biomass followed the Freundlich isotherm, while Langmuir is the best isotherm for RBBR sorption’s elucidation onto AA fungus biomass. The removal of BR18 by BR biomass was found to follow the pseudo-second order. In contrast, the adsorption of RBBR onto the AA biomass followed Lagergren’s pseudo-first order. For both adsorbents, the adsorption was exothermic, feasible, and spontaneous in nature. Finally, the dyes’ biosorption process was perfectly achieved onto fungi biomass via physisorption
Citation - WoS: 1
Citation - Scopus: 1
Cerebrospinal fluid levels of sortilin-1, lipocalin-2, autotaxin, decorin and interleukin-33 in patients with idiopathic intracranial hypertension
(Arquivos de Neuro-Psiquiatria, 2022) Dündar, Ahmet; Arıkanoğlu, Adalet; Özdemir, Hasan Hüseyin; Aslanhan, Hamza; Çevik, Mehmet Uğur
Background Idiopathic intracranial hypertension (IIH) is characterized by increased cerebrospinal fluid (CSF) pressure of unknown cause. It has been suggested that the inflammatory process plays a role in the pathophysiology of the disease. Sortilin-1, lipocalin-2, autotaxin, decorin, and interleukin-33 (IL-33) are among the factors involved in inflammatory processes. Objective To investigate the CSF levels of sortilin-1, lipocalin-2, autotaxin, decorin, and IL-33 in patients with IIH. Methods A total of 24 IIH patients and 21 healthy controls were included in the study. Demographic characteristics of the patients and of the control group as well as CSF pressures were evaluated. Sortilin-1, lipocalin-2, autotaxin, decorin and IL-33 levels in the CSF were measured. Results The CSF levels lipocalin-2, sortilin-1, autotaxin, IL-33 and CSF pressure were significantly higher in the patients group compared with the control group (p < 0.001). Decorin levels were reduced in patients (p < 0.05). There was no correlation between the autotaxin and IL-33 levels and age, gender, CSF pressure, and body mass index. The results of our study showed that inflammatory activation plays an important role in the development of the pathophysiology of IIH. In addition, the fact that the markers used in our study have never been studied in the etiopathogenesis of IIH is important in explaining the molecular mechanism of this disease. Conclusion Studies are needed to evaluate the role of these cytokines in the pathophysiology of the disease. It is necessary to evaluate the effects of these molecules on this process. © 2022. Academia Brasileira de Neurologia. All rights reserved.
Confirmatory test versus screening test analyses for fetal mosaic variations; a large scale study
(Taylor & Francis Online, 2022) Moosavi, Seyed Akbar; Hasannejad-Asl, Behnam; Arami, Masoumeh Kourosh; Nasuti, Mahsa; Oğuz, Mehmet Cemal; Naseri, Abdol-Hossain
Abstract Background Mosaic genetic anomaly is a problematic and interpretative issue in prenatal diagnosis. Conventional karyotyping, as a confirmatory test traditionally used for detecting mosaic and nonmosiac prenatal disorders. Recently Quantitative Fluorescence PCR (QF-PCR) is used for prenatal testing. We retrospectively assessed the frequency of both mosaic and nonmosaic conditions in a large-scale study and compared the clinical value of confirmatory cytogenetic analysis with QF-PCR and other screening tests. Result Of 6033 cases identified as abnormal conditions by sonography or protein marker screening tests, only 180 nonmosaic and 8 mosaic cases confirmed to be abnormal by confirmatory karyotyping test results. The cytogenetic analysis was correlated with other QF-PCR confirmatory test results for nonmosiac conditions but it was not comparable for mosaic cases. Conclusion The cytogenetic analyses were shown to have the greatest clinical value in revealing the various mosaic conditions. The QF-PCR test is shown to be a reliable confirmatory test for nonmosaic diseases but not for mosaicism, and the screening protein marker test can weakly indicate the presence of abnormal cell lines. Moreover, older mothers (>30 years) are at greater risk for developing mosaic ova.
Citation - WoS: 24
Citation - Scopus: 27
Covalent immobilization of benzoylformate decarboxylase from Pseudomonas putida on magnetic epoxy support and its carboligation reactivity
(Elsevier, 2014) Tural; Tarhan; Tural, Bilsen; Tuba; Servet
Epoxy attached magnetic nanoparticles were prepared and used as solid support for covalent immobi-lization and stabilization of benzoylformate decarboxylase (BFD, E.C. 4.1.1.7) fromPseudomonas putida.A three-step immobilization/stabilization procedure is applied. The enzyme is firstly covalently immobi-lized under mild experimental conditions (e.g. pH 7.0, no added MgSO4and 20◦C). Secondly, the enzymeis immobilized under more drastic conditions (higher pH values, higher ionic strengths, etc.) to facili-tate an increase in effective concentration of the enzyme on the support near the epoxide reactive sites.Thirdly, the remaining epoxy groups are blocked to stop any additional interaction between the enzymeand the support. With more drastic conditions, the loading of enzyme can be increased from 1.25 to6.70 mg enzyme per gram of support. The covalently bounded enzyme was characterized in terms ofits activity and stability for the formation of (S)-2-hydroxypropiophenone (2-HPP). The activity of theimmobilized BFD was determined to be 53.0% related to the activity of the free enzyme. The immobilizedbiocatalyst retained 95% of its original activity after five reaction cycles.
Citation - WoS: 3
Citation - Scopus: 3
Determination of hydroxymethylfurfural in Turkish honeys, pekmez (grape molasses), and jam samples by high-performance liquid chromatography with diode array detection
(Wiley Online Library, 2022) Elmastas, Ayhan; Aydin, Firat; Umaz, Adil; Kılınc, Ersin; Arslan, Yasin; aydin, Isil
Hydroxymethylfurfural (HMF) is a chemical contaminant that is not naturally present in carbohydrate-containing food samples. The HMF amounts were changed between 4.8 and 214.1 mg/kg in honey; between 7.8 and 204.0 mg/kg in pekmez (grape molasses); between 5.9 and 22.4 mg/kg in strawberry jam; between 6.0 and 65.4 mg/kg in apricot jam; and between 9.2 and 55.9 mg/kg in cherry found by HPLC. The linear calibration plot of HMF was ranged from 0.05 to 10.0 mu g/ml. The correlation coefficient was found to be 0.99997. LOD and LOQ values were found to be 12.0 and 40.0 ng/ml, respectively. According to the experimental results, HMF amounts of four honey samples, three pekmez (grape molasses) samples, one apricot jam, and one cherry jam for investigated samples were found to be higher than the permission amount of Turkish Food Codex. Novelty impact statement Based on experimental results in this study, if the honey is in a transparent package such as a glass, it should be stored in a dark environment. It would be healthier to prefer molasses produced by reliable brands at low temperatures under vacuum instead of molasses made with traditional methods in open boilers. Generally, since jams with high HMF amount have an overcooked or even burnt flavor, this is an extremely negative situation for consumers.
Citation - WoS: 11
Citation - Scopus: 13
Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II)
(Analytical Biochemistry, 2021) Kılınç, Ersin; Ozdemir, Sadin; Şen, Fatih; Soylak, Mustafa
In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min−1 of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL−1, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
Citation - WoS: 70
Citation - Scopus: 77
Ecofriendly/Rapid Synthesis of Silver Nanoparticles Using Extract of Waste Parts of Artichoke (Cynara scolymus L.) and Evaluation of their Cytotoxic and Antibacterial Activities
(Hindawi, 2021) Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Irtegun Kandemir, Sevgi; Valiyeva, Mahbuba; Mehraliyeva, Sevil; Khalilov, Rovshan; Eftekhari, Aziz
Recycling wastes and providing their use in useful fields attract attention every day. In our study, with the extract prepared from the parts of the Cynara scolymus L. (artichoke) plant that is not suitable for human consumption, silver nanoparticles were easily synthesized in an ec-friendly, energy-free way. Characterization of the obtained nanoparticles was done with a UV-visible spectrophotometer (UV-Vis.), fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM), and zeta potential analysis data. In these data, it was determined that AgNPs have a maximum absorbance at 458.8 nm wavelength, a crystal nanosize of 28.78 nm, and a spherical appearance. The zeta potential of (-) 16.9 mV indicates that silver nanoparticles exhibit a stable structure. Particles show antimicrobial effects on pathogenic species at concentrations of 0.03-0.25 μg/ml, and it was determined by using the minimum inhibition concentration (MIC) microdilution method. By examining their cytotoxic effects on U118, CaCo-2, and Skov-3 cancer cell lines and healthy HDF cell lines by the MTT method, concentrations of inhibitive effects on survival were determined.
Citation - WoS: 24
Citation - Scopus: 28
Economic fast synthesis of olive leaf extract and silver nanoparticles and biomedical applications
(Taylor & Francis Online, 2021) Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Aktepe, Necmettin; Yavuz, Ömer; İrtegun Kandemir, Sevgi
In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and -19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.
The Effect of COVID-19 Fear in Patients and Clinical Nurses on Night Nursing Care
(Sage Journals, 2023) Utli, Hediye; Dinç, Mahmut; Oner, Ugur
This study was conducted with the aim of determining the effect of fear of COVID-19 in patients and clinical nurses on night nursing care. This was a cross-sectional study. The research sample consisted of 604 patients and 270 clinical nurses selected by the convenience sampling method. Data were collected between August 2021 and July 2022 using a Patients’ Information form, a Nurse’s Information form, the Fear of COVID-19 scale, and the Night Nursing Care instrument. No significant difference was found between the total mean scores of the patients and the nurses on the Fear of COVID-19 scale or the Night Nursing Care instrument (rho = –0.017, p = .702; rho = –0.020, p = .741). It was found that patients’ and nurses’ fears of COVID-19 did not affect their perceptions of night nursing care.
Citation - WoS: 2
Citation - Scopus: 5
Evaluation as time-dependent of pesticides applied in pre-harvest period of grown vegetables: removal of pesticide residues in the vegetables
(Taylor & Francis Online, 2022) Elmastas, Ayhan; Umaz, Adil; Pirinc, Vedat; Aydin, Firat
Pesticide use has benefits such as increasing the quality and amount of food required to sustain the human population. However, its harmful effects on the environment and human health are of great concern. The aim of this study was to evaluate concentrations as timedependent of the pesticide and the removal of pesticide residues in vegetable samples with the aid of the QuEChERS 2007.01 procedure. The analytical method has been validated according to SANTE/12682/2019. Good linearity (2.5–100 ng/mL, R2 > 0.99) was observed, and the recovery yields ranged from 94.76% to 104.03%, repeatability (RSDr) ranged from 2.33% to 4.59% and reproducibility (RSDwR) ranged from 4.06% to 8.40%. LOD and LOQ values were determined to be 3.00 ng/g and 10.00 ng/g, respectively. The grown tomato, eggplant and cucumber samples were sprayed with drugs against pests and diseases. Imidacloprid in the grown tomato, cucumber and eggplant samples was determined to be 0.026, 0.186 and 0.096 mg/kg, respectively. Trifloxystrobin was determined to be 0.102, 0.129 and 0.063 mg/kg, respectively. It was determined that pesticide residue in vegetable samples decreases by washing and peeling but does not disappear completely. The peeling process was determined to be more effective than washing. The concentration of pesticide residues in the shell part was found to be hight than in the whole product. Thus, it is important to consume vegetables with their skin peeled and to consume the unpeeled ones by washing them with water at least.
Citation - WoS: 4
Citation - Scopus: 3
A Histopathological, Immunohistochemical and Biochemical Investigation on the in Vitro Antioxidant, Myeloprotective, Hematoprotective and Hepatoprotective Effects of Hypericum Triquetrifolium Seed Extract Against Cyclophosphamide-Induced Toxicity
(Inst Tecnologia Parana, 2019) Sahinturk, Varol; Ayhanci, Adnan; Yildiz, Songul Cetik; Keskin, Cumali
The aim of this study was to investigate in vitro antioxidant properties and in vivo protective effects of the methanol extract of the Hypericum triquetrifolium Turra (HT) seed against acute hepatotoxicity, myelotoxicity and hematotoxicity in rats induced by cyclophosphamide (CP). In order to investigate in vivo protective effects of the HT extract on rat tissues, the rats were divided into nine groups. The toxic effects of CP and the protective effects of HT extract on nucleated cells that are produced by bone marrow, serum alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and oxidative stress index (OSI) levels were investigated biochemically. Additionally, liver tissue samples were examined for histopathological changes and apoptosis by Bcl-2, Bax and caspase-3 immunohistochemistry. The results of this study show that HT seed methanol extract has high total phenolic content (179.52 mu g GAE/mg) and antioxidant activity (87.48% in 500 mu g/mL concentration). CP administration caused hepatotoxicity, myelotoxicity and hematotoxicity in the rats. Whereas, the groups of rats that were injected with different concentrations of HT (25, 50 and 100 mg/kg) and CP (150 mg/kg) showed significant protective effects on bone marrow nucleated cells and important decreases on serum ALT, ALP, LDH and OSI levels were observed when compared with the CP injected group.
Citation - WoS: 17
Citation - Scopus: 14
In-Vitro Antioxidant, Cytotoxic, Cholinesterase Inhibitory Activities and Anti-Genotoxic Effects of Hypericum Retusum Aucher Flowers, Fruits and Seeds Methanol Extracts in Human Mononuclear Leukocytes
(Iranian Journal of Pharmaceutical Research, 2017) Keskin, C.; Keskin, Cumali; Aktepe, N.; Aktepe, Necmettin; Yükselten, Y.; Asuman,; Boğa, M.; 21.02. Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü; 09.01. Department of Nursing / Hemşirelik Bölümü; 9. Faculty of Health Sciences / Sağlık Bilimleri Fakültesi; 21. Vocational School of Health Services / Sağlık Hizmetleri Meslek Yüksekokulu; 01. Mardin Artuklu University / Mardin Artuklu Üniversitesi
The present study investigates the antioxidant, anticancer, anticholinesterase, anti-genotoxic activities and phenolic contents of flower, fruit and seed methanol extracts of Hypericum retusum AUCHER. The amounts of protocatechuic acid, catechin, caffeic acid and syringic acid in methanol extracts were determined by HPLC. Total phenolic content of H. retusum seed extract was found more than fruit and flower extracts. The DPPH free radical scavenging activity of flower and seed methanol extracts showed close activity versus BHT as control. Among three extracts of H. retusum only flower methanol extract was exhibited considerable cytotoxic activities against to HeLa and NRK-52E cell lines. Moreover, seed methanol extract showed both acetyl and butyrl-cholinesterase inhibitory activity. The highest anti-genotoxic effects were seen 25 and 50 μg/mL concentrations. In this study, the extracts showed a strong antioxidant and anti-genotoxic effect. The seed extract was more efficient- than extracts of fruit and flowers. Our results suggest that the antioxidant and anti-genotoxic effects of extracts depend on their phenolic contents. Further studies should evaluate the in-vitro and in-vivo the benefits of H. retusum seed methanol extracts. © 2017 by School of Pharmacy.
Citation - WoS: 11
Citation - Scopus: 7
Investigation into the protective effects of Hypericum Triquetrifolium Turra seed against cyclophosphamide-induced testicular injury in Sprague Dawley rats
(Taylor & Francis Online, 2022) Can, Senanur; Çetik Yıldız, Songül; Keskin, Cumali; Şahintürk, Varol; Cengiz, Mustafa; Appak Başköy, Sıla; Ayhanci, Adnan; Akıncı, Gülsema
For centuries, Turkey has been a significant location here around 80 species of Hypericum with differing names widely occur, which is also known as Turkish folk medicine in treating some bacterial diseases as well as stomach and intestine inflammation. Recent studies have reported this herb family to contain numbers of bioactive compound contents. The study aims to investigate the protective effects of Hypericum triquetrifolium Turra (HT) upon oxidative stress and apoptosis in a rat model in which testes injury was induced by CP. The testicular injury was caused using CP (150 mg/kg). The rats were treated with a single dose (100 mg/kg) of methanol extract of HT to investigate various biochemical markers in the serum and plasma of blood samples apart from assessing the prognosis of CP-induced testicular damage. Added to that, histological analyses were performed to identify possible structural changes and apoptotic indicators, like Bax, Caspase-3, and Bcl-2. In CP Group, there was a rise in the levels of total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), Caspase-3, and Bax while superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), Bcl-2, and total antioxidant capacity (TAC) all decreased. Also, our histological analysis showed damaged testes. On the other hand, neither biochemical nor histological analysis showed testicular damage in HT Alone Group. In CP + HT Group, a significant number of the negatives changes due to CP were observed to have improved remarkably following an HT treatment. This study results suggest that HT could help improve CP-induced testicular injury thanks to its anti-oxidative and anti-apoptotic properties.
Citation - WoS: 7
Citation - Scopus: 7
Investigation of serum vaspin, visfatin, chemerin and IL-18 levels in migraine patients
(Arq Neuropsiquiatr, 2021) Dönder, Ahmet; Cafer, Vugar; Yilmaz, Ahmet; Aslanhan, Hamza; Arikanoğlu, Adalet
Background: Migraines are headaches caused by changes in the trigeminovascular metabolic pathway. Migraine headache attacks are associated with neurovascular inflammation, but their pathophysiological mechanisms have not been fully explained. Objective: To investigate the relationship between serum vaspin, visfatin, chemerin and interleukin-18 (IL-18) levels and the frequency of attacks in migraine headache. Methods: Three groups were established: migraine with aura (n = 50), migraine without aura (n = 50) and control group (n = 50). The migraine diagnosis was made in accordance with the International Classification of Headache Disorders-III beta diagnostic criteria. The analyses on serum vaspin, visfatin, chemerin and IL-18 levels were performed using the enzyme-linked immunosorbent assay method. Results: The serum vaspin, visfatin, chemerin and IL-18 levels were found to be significantly higher in the migraine patients than in the control group (p < 0.01). No statistically signiﬁcant differences in serum vaspin, visfatin, chemerin and IL-18 levels were found among the migraine patients during attacks or in the interictal period (p>0.05). The serum visfatin and chemerin levels of the migraine patients were positively correlated with their serum IL-18 levels (p < 0.01), while their serum chemerin and visfatin levels were positively correlated with their serum vaspin levels (p < 0.05). Conclusions: This study showed that these biomarkers may be related to migraine pathogenesis. Nonetheless, we believe that more comprehensive studies are needed in order to further understand the role of vaspin, visfatin, chemerin and IL-18 levels in the pathophysiology of migraine headaches
Investigation of toxin genes in Staphylococcus aureus strains isolated in Mustafa Kemal University Hospital
(2011) Demir Cemil; Aslantas, Ozkan; Duran, Nızamı; Ocak, S; Özer, Burcın
Aim: The aim of this study was to investigate the presence of genes encoding staphylococcal enterotoxins (SEs), exfoliative toxins (ETAs, ETBs), and toxic shock syndrome toxin-1 (TSST-1) by polymerase chain reaction (PCR) in Staphylococcus aureus strains isolated from various clinical samples from the Mustafa Kemal University Hospital. In addition, PCR-based restriction fragment length polymorphism (RFLP) analysis of the coa gene was employed to genotype the isolates. Materials and methods: A total of 120 S. aureus strains isolated from various clinical samples (blood, wounds, urine, conjuctival swabs, and tracheal aspirate) over a 1 year period, 2007-2008, were used in this study. Results: Almost 65.8% of the isolates possessed at least one toxin gene. The genes most frequently found were seg-sei (40.8%), followed by sea (30%) and eta (19.2%). Overall, 35 toxin genotypes were observed, among which the genotypes seg-sei, sea-seg-sei, and sea-see predominated at the rate of 8.3%, 5.8%, and 5%, respectively. Four coagulase genotype patterns were observed, with molecular sizes ranging from 570 to 970 bp. Coo-based RFLP analysis revealed 7 different patterns using Conclusion: Our results have revealed that toxin genes were very prevalent among S. aureus isolates, and the toxigenic isolates were independent of the genotypes obtained by PCR-RFLP of the coa gene (P > 0.05).
Citation - WoS: 15
Citation - Scopus: 15
Investigations of Hg(II) and Pb(II) tolerance, removal and bioaccumulation and their effects on antioxidant enzymes on thermophilic Exiguobacterium profundum
(Human and Ecological Risk Assessment, 2020) Kılınç, Ersin; Akkoyun, Mahire Bayramoğlu; Özdemir, Sadin
Hg(II) and Pb(II) tolerance, removal, bioaccumulation and effects on antioxidant enzymes of thermophilic Exiguobacterium profundum were investigated. The results indicated that Hg(II) was more toxic than Pb(II) to E. profundum. E. profundum was also more tolerant in solid medium than in liquid medium for Pb(II) and Hg(II). The bacterial growth was not significantly influenced at 1.0 and 2.5 mg/L Pb(II) and Hg(II) for 24 h. The highest Hg(II) and Pb(II) bioaccumulation amounts were determined as 37.56 and 54.35 mg metal/dried bacteria, respectively. Bioaccumulation capacities of the cell membrane of E. profundum for Hg(II) and Pb(II) were determined. The different concentrations of Pb(II) and Hg(II) enhanced the SOD and CAT enzymes. In addition, variations of the surface macrostructure and the functionality of E. profundum after the interaction with Hg(II) and Pb(II) were investigated by the scanning electron microscope (SEM) and the Fourier transform infrared spectroscopy (FT-IR), respectively. This investigation obviously showed that thermophilic E. profundum can also be applied for removal and recovery of toxic metals from industrial wastewater. Clearly, a further investigation should be utilized by thermophilic microorganisms. According to antioxidant enyzme activities, E. profundum can be also used as a bioindicator for the detection of toxic metal pollution in natural water samples.
Citation - WoS: 9
Citation - Scopus: 11
Isolation and Molecular Characterization of Methicillin-Resistant Staphylococci from Horses, Personnel and Environmental Sites at an Equine Hospital in Turkey
(JAPAN SOC VET SCI, 2012) Aslantas, Ozkan; Turkyilmaz, Suheyla; Yilmaz, Mehmet Ali; Erdem, Zeynep; Demir, Cemil
The present study was carried out to assess the frequency of methicillin-resistant staphylococci (MRS) among racehorses (n=209) and veterinary personnel (n=13) as well as environmental surfaces (n=14) at an equine hospital in Adana, Turkey. In addition, species distribution, antimicrobial susceptibility, resistance genes, staphylococcal chromosomal cassette mec (SCCmec) type and clonality of these isolates were also investigated. MRS were identified by 16S rRNA sequencing, and typed by pulsed-field gel electrophoresis (PFGE). As a result, MRS was isolated in horses (48.3%), clinic staff (92.3%) and environmental samples (71.4%). Of the 123 MRS isolates, 118 isolates were identified as Staphylococcus lentus, and the remaining ones were found to be S. sciuri (n=3), S. intermedius (n=1) and S. fleuretti (n=1). All isolates were found to be susceptible against vancomycin, quinupristin-dalfopristin and rifampicin. Additionally, single or various combinations of resistance genes were detected among MRS isolates. SCCmec type II was identified in all isolates. Similar PFGE patterns were observed among MRS isolated from horses, humans, and environmental samples. Since MRS were concurrently isolated from horses and humans it is suggested that cross-transmission of MRS between horses and humans might occur. However, it cannot be ruled out that transmission is human to animal or animal to human.
Citation - WoS: 15
Citation - Scopus: 21
Presence of Biofilm and Adhesin Genes in Staphylococcus aureus Strains Taken from Chronic Wound Infections and their Genotypic and Phenotypic Antimicrobial Sensitivity Patterns
(Photodiagnosis and Photodynamic Therapy, 2020) Demir, Cemil; Demirci, Mehmet; Yigin, Akin; Bahar Tokman, Hrisi; Çetik Yıldız, Songul
The aim of this study was to investigate some biofilm (icaA, icaD and bap) and some adhesion (clfA, fnbA, cna) genes, and also evaluate the phenotypic and genotypic antimicrobial resistance patterns of Staphylococcus aureus strains isolated from wound samples in Mardin, Turkey. A total of 220 wound samples were studied. The biofilm forming ability and resistance pattern for eleven antimicrobial agents were investigated by conventional and multiplex PCR methods. S. aureus was isolated from 112 (50.9%) of 220 wound samples. Moreover, biofilm production was found in 79 (70.5%) of the 112 S. aureus isolates. 97 (86.6%) strains of all isolates were positive for icaA and icaD, and 15 (13.4%) for bap. The adhesin genes, cna, fnbA and clfA were detected in 98 (87.5%), 87 (77.7%), and 75 (66.9%) strains, respectively. The numbers of MSSA and MRSA carrying antimicrobial resistance genes were 19 (16.96%) and 32 (28.57%) for blaZ, 9 (8.04%) and 17 (15.18%) for tetK, 6 (5.36%) and 14 (12.5%) for ermC, 2 (1.79%) and 7 (6.25%) for tetM, 0 (0%) and 5 (4.46%) for mecA, 2 (1.79%) and 4 (3.57%) for ermA, 1 (0.89%) and 2 (1.79 %) for both tetK and tetM, respectively. Our findings indicate that multiplex PCR is a reliable method for identifying biofilm and adhesin producing S. aureus. Our data also provided a nationwide surveillance of the antimicrobial resistance gene profiles of S. aureus for the accurate treatment of patients and to control the dissemination of the resistance genes.
Citation - WoS: 13
Citation - Scopus: 14
Production and characterization of thermostable alpha-amylase from thermophilic Anoxybacillus flavithermus sp nov SO-19
(WILEY-V C H VERLAG GMBH, 2016) Ozdemir, Sadin; Okumus, Veysi; Ulutas, Mehmet Sefa; Dundar, Abdurrahman; Akarsubasic, Alper Tunga; Dumontet, Stefano
This study was concerned with isolation and identification of thermophilic bacteria from hot spring in Afyonkarahisar (Gecek) and optimization of a-amylase production, partial purification of alpha-amylase, and characterization of extracellular enzyme from isolated thermophilic strain 19. To characterize and identify the thermophilic isolated bacteria, morphological analysis and biochemistry tests were studied. Besides, for classification 16S rRNA gene, G-C content and DNA-DNA hybridization analysis were performed. These results indicated that strain 19 is a novel species, Anoxybacillus flavithermus sp. nov. The effects of different fermentation conditions, such as incubation time, temperature, and pH, different carbon and nitrogen sources, and surfactants on a-amylase production were investigated. Various parameters such as temperature and temperature stability, pH and pH stability, detergents and surfactants, different starches, and metal ions on influence of enzyme characterization were assayed. About 93, 87, and 81% of the activities were retained after heating the partially purified enzyme solution at 50, 60, and 70 for 240 min, respectively. Enzyme was excessively inhibited by Hg2+ (6%). The enzyme was activated by Co2+ (212%) and Mg2+ (142%). Enzyme degradated 82% of starch content in apple juice at 70 degrees C in 30 min. The molecular weight of enzyme was estimated as 96 kDa.

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