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Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds
(Molecules, 2022) Alacabey, İhsan
Antibiotics are among the most critical environmental pollutant drug groups. Adsorption is one of the methods used to eliminate these pollutants. In this study, activated carbon was produced from pumpkin seed shells and subsequently modified with KOH. The adsorbent obtained through this procedure was used to remove ciprofloxacin from aqueous systems. Fourier Transform-Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), elemental, X-ray Photoelectron Spectroscopy (XPS), Brunauer–Emmett–Teller (BET) and Zeta analyses were used to characterize the adsorbent. The surface area, in particular, was found to be a very remarkable value of 2730 m2/g. The conditions of the adsorption experiments were optimized based on interaction time, adsorbent amount, pH and temperature. Over 99% success was achieved in removal operations carried out under the most optimal conditions, with an absorption capacity of 884.9 mg·g−1. In addition, the Langmuir isotherm was determined to be the most suitable model for the adsorption interaction
Biological assays, electrochemical behavior, and theoretical DFT calculations of Ru(II) complexes of chiral phosphinite based based on β-amino alcohols: Transfer hyrogenation of ketones using a HCOOH/Et3N mixture
(Elsevier, 2024) Dündar, Abdurrahman; Meriç, Nermin; Ertekin Binbay, Nil; Okumuş, Veysi; Sever, M. Şerif; Belyankova, Yelizaveta; Tursynbek, Saniya; Dauletbakov, Anuar; Sarah, Bayazit; Zolotareva, Darya; Yerassyl, Kamshyger; Dündar, Abdurahman
Synthesis of two phosphinite ligands based on β-amino alcohols, in high yields has been demonstrated. When we treated [Ru(arene)(μ-Cl)Cl]2 {arene:p-cymene,benzene} with chelating phosphinite ligands, we obtained neutral Ru(II)-complexes possessing the general formula [Ru(arene)phosphiniteCl2]. The structure of the ligands and complexes was confirmed using analytical and spectroscopic techniques. The quantum chemical calculations were carried out for the ruthenium complexes at the DFT/CAM-B3LYP level of theory in gas phase. The phosphinite complexes were subjected to cyclic voltammetry studies in order to determine the energies of HOMO and LUMO levels and to estimate their electrochemical and some electronic properties. Organic complex-based memory substrates were immobilized using TiO2-modified ITO electrodes, and the memory functions of phosphinite-based organic complexes were verified by chronoamperometry (CA) and open-circuit potential amperometry (OCPA). In the present study, the antioxidant potentials of ruthenium-based p-cymene and benzene complexes through DPPH radical scavenging, metal chelating, and reducing power activities were also determined. In addition, DNA binding abilities and antimicrobial activities of these complexes against pathogenic bacteria were studied. Finally, the ruthenium complex, (2S)-1-{[(2S)-2-[(diphenylphosphanyl)oxy]propyl][(1R)-1-phenylethyl]amino}propan-2-yldiphenyl phosphinitobis[dichloro(η6-benzene)ruthenium(II)] also catalyzed asymmetric transfer hydrogenation of acetophenone with high conversion (up to 99%) and good enantioselectivity (ee up to 89 %), in the existence of formic acid and triethylamine in dichloromethane medium under air atmosphere.
Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract
(Elsevier, 2023) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Aytuğ Ava, Canan; İrtegün Kandemir, Sevgi; Cebe, Deniz Barış; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan
Metallic nanoparticle biosynthesis is thought to offer opportunities for a wide range of biological uses. The green process of turning biological waste into utilizable products gaining attention due to its economical and eco-friendly approach in recent years. This study reported the ability of Solanum tuberosum (ST) peel extract to the green synthesis of non-toxic, stable, small-sized silver nanoparticles without any toxic reducing agent utilizing the phytochemical components present in its structure. UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, flourier scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and energy dispersive analysis X-ray confirmed the biosynthesis and char-acterization of silver nanoparticles. Also, dynamic light scattering and thermogravimetric ana-lyses showed stable synthesized nanoparticles. The antibacterial activity of the biosynthesized silver nanoparticles was evaluated against four different bacterial strains, Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) Bacillus subtilis (B. subtilis), and a yeast, Candida albicans (C. albicans) using the minimum inhibitory concen-tration technique. The cytotoxic activities were determined against Human dermal fibroblast (HDF), glioblastoma (U118), colorectal adenocarcinoma (CaCo-2), and human ovarian (Skov-3) cell lines cancer cells using MTT test. The nanoparticle capping agents that could be involved in the reduction of silver ions to Ag NPs and their stabilization was identified using FTIR. Nano -particles were spherical in shape and had a size ranging from 3.91 to 27.07 nm, showed crys-talline nature, good stability (-31.3 mV), and the presence of capping agents. ST-Ag NPs significantly decreased the growth of bacterial strains after treatment. The in vitro analysis showed that the ST-Ag NPs demonstrated dose-dependent cytotoxicity against cell lines. Based on the data, it is feasible to infer that biogenic Ag NPs were capped with functional groups and demonstrated considerable potential as antibacterial and anticancer agents for biomedical and industrial applications.
Crystal structure, IR and NMR spectra of (E)-2‑methoxy-4-(2-morpholinovinyl)phenol molecule and its DFT calculations
(ScienceDirect, 2023) Arici, K.; Altınoz, D.E.; Cavusoglu, M.; Diken, S.
In this study; molecular structure, IR and NMR spectroscopic properties of (E)-2–methoxy-4-(2- morpholinovinyl)phenol are investigated both experimentally and theoretically for the first time. The structural characterization of the compound was carried out by X-ray diffraction technique. The bond lengths, bond angles and dihedral angles of the compound were optimized using DFT with the B3LYP 6–311G(dp) base set. Using the optimized results, the infrared vibration frequencies and intensities of the compound as well as the 1H and 13C chemical shift values in the NMR spectrum were calculated with the B3LYP 6–311G(dp) basis set. In addition, the potential energy distributions (PED) of the structure were calculated to determine some characteristic vibrational frequencies of the compound. It is found that the theoretically calculated data support the experimental results. As a result, there is a good agreement between the experimentally determined structural parameters, vibrational frequencies, chemical shifts and theoretically calculated data of the compound.
Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C-60 modified gamma-Fe2O3 superparamagnetic iron oxide nanoparticles
(ROYAL SOC CHEMISTRY, 2015) Kılınç, Ersin
In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C-60 modified maghemite (gamma-Fe2O3 superparamagnetic iron oxide nanoparticles - SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL(-1) with a limit of detection of 0.06 ng mL(-1). The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL(-1). The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL(-1).
Development of Novel Fe3O4/AC@SiO2@1,4-DAAQ Magnetic Nanoparticles with Outstanding VOC Removal Capacity: Characterization, Optimization, Reusability, Kinetics, and Equilibrium Studies
(Industrial and Engineering Chemistry Research, 2021) Ece, Mehmet Şakir; Kutluay, Sinan; Şahin, Ömer; Horoz, Sabit
The adsorption of pollutants to the surface of adsorbents plays a critical role in the effectiveness of adsorption technology for air purification applications. Herein, novel magnetic nanoparticles functionalized with 1,4-diaminoanthraquinone (1,4-DAAQ), namely, Fe3O4/activated carbon (AC)@SiO2@1,4-DAAQ, were innovatively synthesized via co-precipitation and sol-gel techniques. After that, these nanoparticles were used for high-efficiency removal of volatile organic compounds (VOCs) (i.e., benzene and toluene). The synthesized nanoparticles were characterized by various techniques such as Fourier transform IR spectroscopy, thermogravimetric analysis/differential thermal analysis, scanning electron microscopy, and Brunauer-Emmett-Teller analysis. The dynamic adsorption process of VOCs was optimized based on operating parameters. The adsorption experiments revealed that Fe3O4/AC@SiO2@1,4-DAAQ showed exceptional performance for the removal of VOCs. It was observed that for benzene, Fe3O4, AC, Fe3O4/AC, Fe3O4/AC@SiO2, and Fe3O4/AC@SiO2@1,4-DAAQ exhibited dynamic adsorption capacities of 180.25, 228.87, 295.84, 382.10, and 1232.77 mg/g, respectively. Additionally, for toluene, they exhibited dynamic adsorption capacities of 191.08, 274.53, 310.26, 421.30, and 1352.16 mg/g, respectively. This indicated that the modification of 1,4-DAAQ could greatly enhance the dynamic adsorption capacity of Fe3O4/AC@SiO2@1,4-DAAQ for VOCs. In addition to the apparent adsorptive behavior in removing VOCs, Fe3O4/AC@SiO2@1,4-DAAQ exhibited high repeatability. After ten consecutive adsorption/desorption cycles, for benzene and toluene, Fe3O4/AC@SiO2@1,4-DAAQ retained 79.36 and 78.24% of its initial adsorption capacity, respectively. According to the characterization results, the average pore diameter for Fe3O4/AC@SiO2@1,4-DAAQ was determined to be 24.46 nm, indicating that they were in the mesopore range. The adsorption mechanism of the VOCs on Fe3O4/AC@SiO2@1,4-DAAQ was clarified by investigating the isotherm and kinetic criteria in detail. Isotherm models suggested that the adsorption process of VOCs is physical. Moreover, from the analysis of diffusion-based rate-limiting kinetic models, the findings reveal a combination of intraparticle diffusion as well as film diffusion throughout the adsorption process of VOCs. In addition, it was concluded from the analysis of the mass transfer model factors that global mass transfer and internal diffusion are more effective than film diffusion. The results demonstrated that the Fe3O4/AC@SiO2@1,4-DAAQ nanoadsorbent is a promising material for the effective removal of VOCs.
The effect of mindfulness on medication adherence in individuals diagnosed with Schizophrenia: A cross-sectional study
(PubMed, 2022) Yaşar Can, Sevinç; Dilmen Bayar, Behiye; Erkan, Fatma Melike; Dilmen Bayar, Behiye; Yaşar Can, Sevinç; Şanlı, Mehmet Emin
Objective: The aim of this study is to determine the effect of mindfulness on medication adherence in individuals diagnosed with schizophrenia. Materials and methods: This cross-sectional study was conducted with 147 individuals diagnosed with schizophrenia. The data were collected using the Descriptive Characteristics Form, Morisky Medication Adherence Scale, and Mindful Attention Awareness Scale. Results: A strong positive correlation was determined between the mindfulness level and medication adherence of the individuals diagnosed with schizophrenia. Furthermore, mindfulness of the individuals diagnosed with schizophrenia was statistically significant in explaining medication adherence (p < 0.05) and mindfulness predicted medication adherence by 64%. Conclusion: It was observed that the individuals' mindfulness level was low and half of the participants had low medication adherence. Moreover, as their mindfulness level increased, so did their medication adherence.
Efficacy of antimicrobial peptide LL-37 against biofilm forming Staphylococcus aureus strains obtained from chronic wound infections
(Elsevier, 2022) Demir, Cemil; Yigin, Akin; Demir, Cemil
The antimicrobial peptide LL-37 showed inhibitory effects against Staphylococcus aureus strains, which often responsible for wound infections. Understanding the molecular mechanisms of biofilm-containing wound infections is important. Thus, this study aimed to investigate both the antimicrobial and biofilm efficacy of LL-37 against biofilm-positive methicillin-susceptible S. aureus (MSSA) strains and biofilm-positive methicillin-resistant S. aureus (MRSA) strains obtained from chronic wound infections and its effect on different quorum sensing and virulence genes at suboptimal concentrations. Fifteen biofilm-forming MRSA and 15 biofilm-forming MSSA strains were included in this study. The minimum inhibitory concentration (MIC) values and biofilm formation were tested by microdilution methods. Real-time PCR was performed to determine gene expression levels. MIC values for LL-37 were 89.6 mg/L and 132.3 mg/L for MSSA and MRSA strains, respectively. No statistically significant difference was found between MRSA and MSSA strains in terms of the effect of LL-37 on biofilm formation. A statistically significant difference was found between MRSA and MSSA strains for atlA, RNAIII, and agrA gene expression levels following exposure to a suboptimal concentration of LL-37. Ultimately, the required LL-37 antimicrobial concentration was quite high; however, LL-37 antibiofilm concentration may be acceptable for use in humans against biofilm-forming MRSA and MSSA strains. This is the first study to investigate to effect of a suboptimal LL-37 concentration on gene expression levels of biofilm-forming MSSA and MRSA strains. LL-37 affected quorum sensing and biofilm producing mechanisms, even at suboptimal MIC concentrations.
Enantioseparation of Mandelic Acid Enantiomers With Magnetic Nano-Sorbent Modified by a Chiral Selector
(Wiley Online Library, 2015) Tarhan; Tural; Tural; Topal;, Tuba; Bilsen; Servet; Giray
In this study,R(+)-α-methylbenzylamine-modified magnetic chiral sorbent wassynthesized and assessed as a new enantioselective solid phase sorbent for separation ofmandelic acid enantiomers from aqueous solutions. The chemical structures and magnetic prop-erties of the new sorbent were characterized by vibrating sample magnetometry, transmissionelectron microscopy, Fourier transform infrared spectroscopy, and dynamic light scattering.The effects of different variables such as the initial concentration of racemic mandelic acid, dos-age of sorbent, and contact time upon sorption characteristics of mandelic acid enantiomers onmagnetic chiral sorbent were investigated. The sorption of mandelic acid enantiomers followeda pseudo-second-order reaction and equilibrium experiments were wellfitted to a Langmuir iso-therm model. The maximum adsorption capacity of racemic mandelic acid on to the magneticchiral sorbent was found to be 405 mg g 1. The magnetic chiral sorbent has a greater affinityfor (S)-(+)-mandelic acid compared to(R)-( )-mandelic acid. The optimum resolution wasachieved with 10 mL 30 mM of racemic mandelic acid and 110 mg of magnetic chiral sorbent.The best percent enantiomeric excess values (up to 64%) were obtained by use of a chiralpakAD-H column.
Endosulfan Elimination Using Amine-Modified Magnetic Diatomite as an Adsorbent
(Frontiers in Chemistry, 2022) Alacabey, İhsan
Pesticides are among the most dangerous developing toxins since they are very hazardous to the environment and threaten human health. In this study, researchers successfully manufactured surface-modified magnetic diatomite (m-DE-APTES) and used them as a sorbent to extract endosulfan from an aqueous solution. There is no other study like it in the scholarly literature, and the results are astounding. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), electron spin resonance (ESR), and surface area measurements were used to analyze magnetic diatomite particles with surface modification. According to the analysis results, magnetic diatomite has a wide surface area and a porous structure. Furthermore, m-DE-APTES has a higher endosulfan adsorption capacity (97.2 mg g-1) than raw diatomite (DE) (16.6 mg g-1). Adsorption statistics agree with Langmuir adsorption isotherm (R 2 = 0.9905), and the adsorption occurred spontaneously at -2.576 kj mol-1 in terms of ΔGo. Finally, m-DE-APTES are a viable alternative adsorbent for removing pesticides from aqueous solutions.
Fabrication and characterization of 3,4-diaminobenzophenone-functionalized magnetic nanoadsorbent with enhanced VOC adsorption and desorption capacity
(Environmental Science and Pollution Research, 2021) Ece, Mehmet Şakir; Şahin, Ömer; Kutluay, Sinan; Horoz, Sabit
The present study, for the first time, utilized 3,4-diaminobenzophenone (DABP)-functionalized Fe3O4/AC@SiO2 (Fe3O4/AC@SiO2@DABP) magnetic nanoparticles (MNPs) synthesized as a nanoadsorbent for enhancing adsorption and desorption capacity of gaseous benzene and toluene as volatile organic compounds (VOCs). The Fe3O4/AC@SiO2@DABP MNPs used in adsorption and desorption of benzene and toluene were synthesized by the co-precipitation and sol-gel methods. The synthesized MNPs were characterized by SEM, FTIR, TGA/DTA, and BET surface area analysis. Moreover, the optimization of the process parameters, namely contact time, initial VOC concentration, and temperature, was performed by applying response surface methodology (RSM). Adsorption results demonstrated that the Fe3O4/AC@SiO2@DABP MNPs had excellent adsorption capacity. The maximum adsorption capacities for benzene and toluene were found as 530.99 and 666.00 mg/g, respectively, under optimum process parameters (contact time 55.47 min, initial benzene concentration 17.57 ppm, and temperature 29.09 °C; and contact time 57.54 min, initial toluene concentration 17.83 ppm, and temperature 27.93 °C for benzene and toluene, respectively). In addition to the distinctive adsorptive behavior, the Fe3O4/AC@SiO2@DABP MNPs exhibited a high reproducibility adsorption and desorption capacity. After the fifth adsorption and desorption cycles, the Fe3O4/AC@SiO2@DABP MNPs retained 94.4% and 95.4% of its initial adsorption capacity for benzene and toluene, respectively. Kinetic and isotherm findings suggested that the adsorption mechanisms of benzene and toluene on the Fe3O4/AC@SiO2@DABP MNPs were physical processes. The results indicated that the successfully synthesized Fe3O4/AC@SiO2@DABP MNPs can be applied as an attractive, highly effective, reusable, and cost-effective adsorbent for the adsorption of VOC pollutants. Graphical abstract[Figure not available: see fulltext.]
Fabrication and characterization of Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide magnetic nanomaterials
(SpringerLink, 2022) Ece, Mehmet Şakir; Şahin, Ömer; Ece, Mehmet Şakir
Abstract In this study, the fabrication of perlite-supported Fe3O4 (Fe3O4/perlite), SiO2-coated Fe3O4/perlite (Fe3O4/perlite@SiO2), and sulfanilamide-modified Fe3O4/perlite@SiO2 (Fe3O4/perlite@SiO2@sulfanilamide) magnetic nanomaterials and their characterization by various spectroscopic techniques were presented. For this purpose, first, Fe3O4/perlite was fabricated via the co-precipitation method. Then, Fe3O4/perlite@SiO2 and Fe3O4/perlite@SiO2@sulfanilamide nanomaterials were fabricated using the sol–gel method. The structural properties of the fabricated nanomaterials were characterized using Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), SEM-energy-dispersive X-ray spectroscopy (EDX), thermogravimetric analysis-differential thermal analysis, and X-ray diffraction (XRD) analyses. The SEM, SEM–EDX, FTIR, and XRD analyses revealed that the fabrication and surface coatings of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were successfully performed. It was concluded that the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide showed a type IV-H3 hysteresis loop according to the International Union of Pure and Applied Chemistry classification. According to the BET analysis, it was found that the specific surface areas of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 8.09, 12.71, and 5.89 m2/g, respectively. The average pore radius of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 9.68, 7.91, and 34.69 nm, respectively, using the Barrett-Joyner-Halenda method. Moreover, the half-pore widths of the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were 2.27, 1.58, and 17.99 nm, respectively, using the density functional theory method. Furthermore, in light of characterization findings, the Fe3O4/perlite, Fe3O4/perlite@SiO2, and Fe3O4/perlite@SiO2@sulfanilamide were in crystalline cubic spinel form, and they had mechanical and thermal stability and a mesoporous structure. Within the framework of the results, these developed nanomaterials, which have potential in many applications, such as sustainable technologies and environmental safety technologies, were brought to the attention of related fields.
Facile synthesis and comprehensive characterization of Ni-decorated amine groups-immobilized Fe3O4@SiO2 magnetic nanoparticles having enhanced solar cell efficiency
(Journal of Materials Science: Materials in Electronics, 2021) Ece, Mehmet Şakir; Ekinci, Arzu; Kutluay, Sinan; Şahin, Ömer; Horoz, Sabit
In this study, the synthesis and comprehensive characterization of Fe3O4@SiO2 magnetic nanoparticles (MNPs) immobilized with L-Arginine decorated with nickel (Ni) was achieved, and their ability in solar cell efficiency was evaluated. Fe3O4, Fe3O4@SiO2, Fe3O4@SiO2@L-Arginine and Fe3O4@SiO2@L-Arginine-Ni MNPs were prepared by co-precipitation and sol-gel methods. The structural, morphological, optical and textural properties of the prepared MNPs were clarified by Fourier Transform Infrared Spectroscopy (FTIR), Energy-Dispersive X-Ray (EDX), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Brunauer–Emmett–Teller (BET), Thermal Gravimetric Analysis (TGA) and Ultraviolet-Visible (UV-Vis) analyzes. From the BET data, it is understood that the specific surface area of the prepared Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@L–Arginine MNPs is 60.85, 28.99 and 29.84 m2/g, respectively. From the pore size distribution determined by Barrett-Joyner-Halenda (BJH) method, it was understood that the pore radius of Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@L–Arginine MNPs were in the range of mesopore and the average pore radius was equal to approximately 11.03, 9.11 and 28.45 nm, respectively. It is assumed that the half pore widths calculated by the density functional theory (DFT) method of the prepared of Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@L-Arginine MNPs are 5.58, ∼ 0.88, and ∼ 17.98 nm, respectively. The energy band gap of the prepared MNPs with spinel structure was determined as approximately 3.10 eV. In addition to the structural, morphological, optical and textural properties, the photovoltaic properties of the prepared MNPs were examined. Au/CuO/Fe3O4@SiO2@L–Arginine-Ni/ZnO/SnO2:F solar cell device was created by using existing Fe3O4@SiO2@L–Arginine-Ni MNPs as buffer layer. The power conversion efficiency (%) of the prepared Fe3O4@SiO2@L–Arginine-Ni MNPs based solar cell device was calculated as 1.84 %. This numerical result shows that the prepared Fe3O4@SiO2@L-Arginine-Ni MNPs can be used as a promising buffer layer in a solar structure.
gamma-Fe2O3 magnetic nanoparticle functionalized with carboxylated multi walled carbon nanotube: Synthesis, characterization, analytical and biomedical application
(ELSEVIER SCIENCE BV, 2016) Kilinc, Ersin
In recent years, magnetic nanoparticles attained special interest in nanobiotechnology and nanomedicine due to their uniqe properties and biocompatibilities. From this perspective, hybride nanostructure composed from gamma-Fe2O3 magnetic nanoparticle and carboxylated multi walled carbon nanotube was synthesized and characterized by FT-IR, VSM, SEM, HR-TEM and ICP-OES. Microscopy images showed that magnetic nanoparticles were nearly spherical structure that arranged on the axis of carboxylated MWCNT. Particle size was found lower than 10 nm. VSM results showed that the obtained magnetic nanoparticles presented superparamagnetic properties at room temperature. The magnetic saturation value was determined as 35.2 emu/g. It was used for the adsorption and controlled release of harmane, a potent tremor-producing neurotoxin. Maximum adsorption capacity was calculated as 151.5 mg/g from Langmuir isotherm. Concentration of harmane was determined by HPLC with fluorescence detection. The antimicrobial activity of synthesized magnetic nanoparticle was investigated against gram-negative and gram-positive bacteria. However, no activity was observed. (C) 2015 Elsevier B.V. All rights reserved.
Green synthesis of silver nanoparticles mediated Diospyros kaki L. (Persimmon): determination of chemical composition and evaluation of their antimicrobials and anticancer activities
(Frontiers in Chemistry, 2023) Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Baran, Mehmet Fırat; Eftekhari, Aziz; Gareev, Ilgiz
The eco-friendly synthesis of metallic nanoparticles (MNPs) using biological materials is an encouraging and innovativeness approach to nanotechnology. Among other synthesizing methods, biological methods are chosen because of their high efficiency and purity in many aspects. In this work, using the aqueous extract obtained from the green leaves of the D. kaki L. (DK); silver nanoparticles were synthesized in a short time and simply with an eco-friendly approach. The properties of the synthesized silver nanoparticles (AgNPs) were characterized using various techniques and measurements. In the characterization data of AgNPs, Maximum absorbance at 453.34 nm wavelengths, the average size distribution of 27.12 nm, the surface charge of -22.4 mV, and spherical appearance were observed. LC-ESI-MS/MS analysis was used to assess the compound composition of D. kaki leaf extract. The chemical profiling of the crude extract of D. kaki leaves revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the identification of five major high-feature compounds: two major phenolic acids (Chlorogenic acid and Cynarin), and tree flavonol glucosides (hyperoside, quercetin-3-glucoside, and quercetin-3- D-xyloside). The components with the highest concentrations were cynarin, chlorogenic acid, quercetin-3- D-xyloside, hyperoside, and quercetin-3-glucoside, respectively. Antimicrobial results were determined by a MIC assay. The biosynthesized AgNPs exhibited strong antibacterial activity against the human and food pathogen Gram (+ and -) bacteria and good antifungal activity against pathogenic yeast. It was determined that 0.03-0.050 μg/mL concentrations ranges of DK-AgNPs were growth suppressive concentrations on all pathogen microorganisms. The MTT technique was used to study the cytotoxic effects of produced AgNPs on cancer cell lines (Glioblastoma (U118), Human Colorectal Adenocarcinoma (Caco-2), Human Ovarian Sarcoma (Skov-3) cancer cell lines, and Human Dermal Fibroblast (HDF) healthy cell line). It has been observed that they have a suppressive effect on the proliferation of cancerous cell lines. After 48 h of treatment with Ag-NPs, the DK-AgNPs were found to be extremely cytotoxic to the CaCo-2 cell line, inhibiting cell viability by up to 59.49% at a concentration of 50 g mL-1. It was found that the viability was inversely related to the DK-AgNP concentration. The biosynthesized AgNPs had dose-dependent anticancer efficacy. Because of the high concentration of bioactive chemicals in Diospyros kaki, it may be employed as a biological resource in medicinal applications. DK-AgNPs were shown to be an effective antibacterial agent as well as a prospective anticancer agent. The results provide a potential approach for the biogenic production of DK-AgNPs utilizing D. kaki aqueous leaf extract.
Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide
(Elsevier GmbH, 2019) Çetik Yıldız, Songül; Demir, Cemil; Demir, Cemil; Kulcanay Şahin, İlknur; Teksoy, Özgün; Ayhancı, Adnan
This study aims to examine cyclophosphamide (CP) exsposure associated toxicity on rat livers and the likely defensive effects of boric acid (BA). The rats used in this study were divided into four groups: control group, CP group, BA group, and BA + CP group. The present study was carried out using routine histological H&E stain, immunohistochemical stain caspase-3 as apoptotic marker, serum biochemical analysis for liver function markers (alanine transaminase (ALT), aspartate transaminase (AST) and alkalen phosphatase (ALP)), oxidative stress markers (total oxidant status (TOS), oxidative stress index (OSI) and total antioxidant capacity marker (TAC)). In the CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 increased whereas TAC levels decreased compared with the control group. In the BA + CP group, the levels of ALT, AST, ALP, TOS, OSI and caspase-3 decreased whereas TAC levels increased compared with the CP group. The histopathological evaluation of light microscope images and immunohistochemical caspase-3 activity in the BA + CP group were found to be decrease compared with those in the CP group. In conclusion, BA was successful in defending the liver against apoptosis and histopathological changes that are attributable to CP. © 2019 Elsevier GmbH
Investigation into the protective effects of Hypericum Triquetrifolium Turra seed against cyclophosphamide-induced testicular injury in Sprague Dawley rats
(Taylor & Francis Online, 2022) Çetik Yıldız, Songül; Keskin, Cumali; Keskin, Cumali; Şahintürk, Varol; Cengiz, Mustafa; Appak Başköy, Sıla; Ayhanci, Adnan; Akıncı, Gülsema
For centuries, Turkey has been a significant location here around 80 species of Hypericum with differing names widely occur, which is also known as Turkish folk medicine in treating some bacterial diseases as well as stomach and intestine inflammation. Recent studies have reported this herb family to contain numbers of bioactive compound contents. The study aims to investigate the protective effects of Hypericum triquetrifolium Turra (HT) upon oxidative stress and apoptosis in a rat model in which testes injury was induced by CP. The testicular injury was caused using CP (150 mg/kg). The rats were treated with a single dose (100 mg/kg) of methanol extract of HT to investigate various biochemical markers in the serum and plasma of blood samples apart from assessing the prognosis of CP-induced testicular damage. Added to that, histological analyses were performed to identify possible structural changes and apoptotic indicators, like Bax, Caspase-3, and Bcl-2. In CP Group, there was a rise in the levels of total oxidant status (TOS), malondialdehyde (MDA), oxidative stress index (OSI), Caspase-3, and Bax while superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), Bcl-2, and total antioxidant capacity (TAC) all decreased. Also, our histological analysis showed damaged testes. On the other hand, neither biochemical nor histological analysis showed testicular damage in HT Alone Group. In CP + HT Group, a significant number of the negatives changes due to CP were observed to have improved remarkably following an HT treatment. This study results suggest that HT could help improve CP-induced testicular injury thanks to its anti-oxidative and anti-apoptotic properties.
Investigation of the effectiveness of waste nut shell–based hydrochars in water treatment: a model study for the adsorption of methylene blue
(SpringerLink, 2022) Demirel, Mehmet Ferit; Alacabey, İhsan; Alacabey, İhsan; Yabalak, Erdal
Thousands of tons of walnut and pistachio green outer shells emerge as waste sources. Recycling such wastes in environmental applications is of great importance. In this study, the efciency of waste walnut and pistachio shell–based hydrochars in removing methylene blue (MB), which represents hazardous chemicals, from the water was investigated. Outer green pistachio shell–based hydrochar (PH) and outer green walnut shell–based hydrochar (WH) were characterized by FT-IR, SEM, EDX, TG–DTA, and BET analyses. The adsorption of MB was carried out at diferent concentrations and temperatures using WH and PH, and the adsorption parameters of Langmuir and Freundlich were investigated. The R2 values of PH were calculated as 0.9963, 0.9974, and 0.9950 and of WH adsorbent were calculated as 0.9759, 0.9939, and 0.9981 for the MB adsorption at 298 K, 313 K, and 323 K, respectively. The separation factor (RL) values for WH and PH were calculated as 0.1650≥RL≥0.103, 0.1108≥RL≥0.0177, respectively. Both adsorbents ft the Langmuir model. The ΔH° values of the WH and PH adsorbents were found to be 37.0940 and 22.2493, respectively. Positive ΔH° values indicated that the adsorption was endothermic. The negative ΔG° values of both adsorbents indicated a spontaneous adsorption process. It was shown that waste nut shell–based hydrochars can be used efectively in water treatment.
Magnetic diatomite for pesticide removal from aqueous solution via hydrophobic interactions
(Springer Link, 2019) Alacabey, İhsan; Yıldız, Emrecan; Alacabey, İhsan; Karabörk, Muharrem; Uzun, Lokman
Pesticides are highly hazardous chemicals for the environment and human health and their use in agriculture is constantly increasing. Although 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane 4,4′-DDT was banned at developed countries, it is still one of the most dangerous of chemical due to accumulation in the environment. It is known that the toxicity of DDT affects some enzyme systems biochemically. The main motivation of this study is to develop an effective adsorbate for the removal DDT, which was chosen as a model hydrophobic pesticide, out of aqueous systems. For this purpose, the bare diatomite particles were magnetically modified and a hydrophobic ligand attached to enhance its adsorptive and physio-chemical features. Under optimal conditions, a high adsorption capacity, around 120 mg/g with the hydrophobic and magnetic diatomite particles, modification of the diatomite particles reduced average pores diameter whereas surface area and total pore volume increased (around 15-folds). After five consecutive adsorption–desorption cycles, no significant decrease in adsorption capability was observed. The adsorption isotherms (Langmuir, Freundlich, and Flory–Huggins) applied to the data indicated that the adsorption process occurred via monolayer adsorption in an entropy-driven manner. The kinetic data also revealed the quick adsorption process without any diffusion limitations
Metallo and metal free phthalocyanines bearing (4-(1(4-phenoxyphenyl)-1-phenylethyl)phenol substituents: Synthesis, characterization, aggregation behavior, electronic, antioxidant and antibacterial properties
(ELSEVIER SCIENCE SA, 2014) Agirtas, M. Salih; Guven, M. Emin; Gumus, Selcuk; Ozdemir, Sadin; Dundar, Abdurrahman
As starting material the phthalonitrile derivative bearing (4-(1(4-phenoxyphenyl)-1-phenylethyl)phenol substituents at peripheral position was prepared by a nucleophilic displacement reaction. Cyclotetramerization of 4-(4-(1-(4-hydroxyyphenyl)-1-phenylethyl)phenoxy)phthalonitrile derivative in the presence of corresponding metal salts gave the new metallophthalocyanines. Metal free phthalocyanine was obtained from the reaction of 4-(4-(1-(4-hydroxyyphenyl)-1-phenylethyl)phenoxy)phthalonitrile units. The novel compounds have been characterized by using various spectroscopic data. The aggregation investigations carried out in different concentrations indicate that 4-(4-(1-(4-hydroxyyphenyl)-1-phenylethyl)phenoxy)phthalocyanine compounds do not have any aggregation behavior for the concentration range of 10(-4)-10(-5) M in tetrahydrofuran. The antioxidant activities of novel compounds were analyzed through radical scavenging ability of 1,1-dipheny1-2-picrylhydrazyl, chelating ability to ferrous ions and reducing power. In addition to these, the antibacterial activities of compounds were investigated. Moreover, the ground-state geometries of the complexes were optimized using B3LYP/6-31G(d,p) level of density functional theory in order to predict the three-dimensional geometries and electronic structure. (C) 2014 Elsevier B.V. All rights reserved.

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