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Kılınç, Ersin

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Kılınç E.
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Doç. Dr.
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Department of Medical Services and Techniques / Tıbbi Hizmetler ve Teknikleri Bölümü
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Former Staff
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Scholarly Output

7

Articles

7

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0

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0

Scholarly Output Search Results

Now showing 1 - 6 of 6
  • Article
    Anason (Pimpinella anisum L.) ve kimyon (Cuminum cyminum L.) tohumlarının uçucu yağ kompozisyonu ile antimikrobiyal ve antioksidan özelliklerinin belirlenmesi
    (2014) Tolan, Veysel; Kılınç, Ersin; Kızıl, Süleyman; Kılınç, Ersin
    Bu çalışmada, anason (Pimpinella anisum L.) ve kimyon (Cuminum cyminum L.) tohumlarının uçucu yağ bileşenleri ile bu yağların antimikrobiyal ve antioksidan özelliklerinin belirlenmesi amaçlanmıştır. Uçucu yağ bileşenleri GC/MS cihazı ile belirlenmiştir. Yağların antimikrobiyal özellikleri disk difüzyon yöntemi ile Gram pozitif (Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615), Gram negatif (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) bakterileri ve maya (Candida albicans ATCC 10231) kullanılarak, antioksidan özellikler ise DPPH yöntemiyle belirlenmiştir. Anason uçucu yağının esas bileşeninin trans-anetol (% 52.94) olduğu, bunu iso-anetol (% 13.89), karyofillen oksit (% 8.55) ve karyofillen (% 4.29)’in izlediği; kimyon uçucu yağının ise sırası ile ?-pinen (% 15.77), ?- terpinen (% 15.52), 1-fenil-1-butanol (% 15.13) ve kuminik aldehit (% 12.74) içerdiği saptanmıştır. Anason uçucu yağının mikroorganizmalar üzerinde düşük, kimyon uçucu yağının ise orta derecede antimikrobiyal aktivite gösterdiği saptanmıştır. Kimyon uçucu yağı C. albicans’a karşı 22±0.9 mm’lik inhibisyon zon çapı ile yüksek antimikrobiyal aktivite göstermiştir. P. aeruginosa her iki uçucu yağa karşı direnç göstermiştir. Kimyon uçucu yağının antioksidan aktivitesi (% 75.60) askorbik asit aktivitesine (% 78.75) yakın, BHA (% 50.45) ve BHT (% 23.54) aktivitesinden daha yüksek bulunmuştur. Anason düşük antioksidan aktivite (% 23.24) göstermiş olmasına rağmen BHT ile benzer aktivite göstermiştir. Çalışmada kullanılan örneklerin aktivite sıralaması askorbik asit>kimyon>BHA>BHT>anason şeklinde gerçekleşmiştir.
  • Article
    Boletus edulis loaded with gamma-Fe2O3 nanoparticles as a magnetic sorbent for preconcentration of Co(II) and Sn(II) prior to their determination by ICP-OES
    (SPRINGER WIEN, 2018) Kılınç, Ersin; Yalçın, M. Serkan; Kılınç, Ersin; Soylak, Mustafa
    The authors show that the fungus Boletus edulis loaded with gamma-Fe2O3 nanoparticles is a viable sorbent for magnetic solid phase extraction of trace levels of Co(II) and Sn(II). The surface structure of immobilized magnetized B. edulis was characterized by FT-IR, SEM and EDX. Experimental parameters were optimized. Following elution with 1 M HCl, the ions were quantified by ICP-OES. The limits of detection are 21 pg.mL(-1) for Co(II), and 19 pg.mL(-1) for Sn(II). The preconcentration factors are 100 for both ions. The sorption capacities of the sorbent are 35.8 mg.g(-1) for Co(II) and 29.6 mg.g(-1) for Sn(II). The method was applied to the analysis of certificated reference materials and gave >= 95% recoveries with low RSDs. It was also successfully applied to the quantification of Co(II) and Sn(II) in spiked environmental and food samples.
  • Article
    Preconcentrations of Pb(II), Ni(II) and Zn(II) by solid phase bio-extractor using thermophilic Bacillus subtilis loaded multiwalled carbon nanotube biosorbent
    (ScienceDirect, 2023) Dündar, Abdurrahman; Kılınç, Ersin; Dizge, Nadir; Kılınç, Ersin; Balakrishnan, Deepanraj; Prasad, Kashi Sai; Senthilkumar, Natarajan
    An alternative biotechnological solid phase bio-extraction (SPE) method was developed. Bacillus subtilis loaded multiwalled carbon nanotube was designed and used as biosorbent for the preconcentrations of Pb(II), Ni(II), and Zn(II). The experimental parameters such as sample flow rate, pH of sample solution, amounts of Bacillus subtilis and multiwalled carbon nanotube, volume of sample solution and reusability of column which affects the analytical characteristics of the SPE method were investigated in details. Surface structures were investigated by using FTIR, SEM. The best pH was determined as 5.0 and the percentages recoveries of Zn(II), Ni(II), and Pb(II) were determined as 99.1%, 98.7%, and 96.2%, respectively, at a flow rate of 3 mL/min. In this study, in which the profitable sample volume was determined as 400 mL and the amount of multiwalled carbon nanotube (MWCNT) as 50 mg. It was also observed that the column had a significant potential to preconcentrate Zn(II), Ni(II), and Pb(II) even after 25 reuses. The biosorption capacities for Pb(II), Ni(II) and Zn(II) were calculated as 34.2 mg/g, 36.8 mg/g and 45.5 mg/g respectively. The LOD values were calculated as 0.024 ng/mL for Pb(II), 0.029 ng/mL for Ni(II), and 0.019 ng/mL for Zn(II). The linear range was detected as 0.25–25 ng/mL. The concentrations of Pb(II), Ni(II), and Zn(II) in a variety of real food samples were determined by using developed method after application of certified reference sample.
  • Article
    Development of Armillae mellea immobilized nanodiamond for the preconcentrations of Cr(III), Hg(II) and Zn(II)
    (Analytical Biochemistry, 2021) Kılınç, Ersin; Ozdemir, Sadin; Şen, Fatih; Soylak, Mustafa
    In this study, we present an environmental friend and easy procedure for simultaneous preconcentration of Cr(III), Hg(II) and Zn(II) by solid-phase extraction before their determination by inductively coupled plasma optical emission spectrometry. Armillae mellea immobilized nanodiamond was used as sorbent. During the study, critical parameters influencing the extraction performance were investigated in detail. The best parameters were found as pH 5.0, 2.0 mL min−1 of flow rate, 200 mg of Armillae mellea, 300 mL of sample volume. LOD values were found as 0.025, 0.13 and 0.038 ng mL−1, respectively for Cr(III), Hg(II) and Zn(II). By applying the developed procedure, sensitivities of ICP-OES were improved for 60 fold for Cr(III), Hg(II) and Zn(II). Their concentrations in different food samples were measured after microwave digestion and solid-phase extraction.
  • Article
    Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C-60 modified gamma-Fe2O3 superparamagnetic iron oxide nanoparticles
    (ROYAL SOC CHEMISTRY, 2015) Kılınç, Ersin
    In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C-60 modified maghemite (gamma-Fe2O3 superparamagnetic iron oxide nanoparticles - SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL(-1) with a limit of detection of 0.06 ng mL(-1). The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL(-1). The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL(-1).
  • Article
    Investigations of Hg(II) and Pb(II) tolerance, removal and bioaccumulation and their effects on antioxidant enzymes on thermophilic Exiguobacterium profundum
    (Human and Ecological Risk Assessment, 2020) Kılınç, Ersin; Akkoyun, Mahire Bayramoğlu; Özdemir, Sadin
    Hg(II) and Pb(II) tolerance, removal, bioaccumulation and effects on antioxidant enzymes of thermophilic Exiguobacterium profundum were investigated. The results indicated that Hg(II) was more toxic than Pb(II) to E. profundum. E. profundum was also more tolerant in solid medium than in liquid medium for Pb(II) and Hg(II). The bacterial growth was not significantly influenced at 1.0 and 2.5 mg/L Pb(II) and Hg(II) for 24 h. The highest Hg(II) and Pb(II) bioaccumulation amounts were determined as 37.56 and 54.35 mg metal/dried bacteria, respectively. Bioaccumulation capacities of the cell membrane of E. profundum for Hg(II) and Pb(II) were determined. The different concentrations of Pb(II) and Hg(II) enhanced the SOD and CAT enzymes. In addition, variations of the surface macrostructure and the functionality of E. profundum after the interaction with Hg(II) and Pb(II) were investigated by the scanning electron microscope (SEM) and the Fourier transform infrared spectroscopy (FT-IR), respectively. This investigation obviously showed that thermophilic E. profundum can also be applied for removal and recovery of toxic metals from industrial wastewater. Clearly, a further investigation should be utilized by thermophilic microorganisms. According to antioxidant enyzme activities, E. profundum can be also used as a bioindicator for the detection of toxic metal pollution in natural water samples.