SYNTHESIS, CHARACTERIZATION AND INVESTIGATION OF ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLES FROM CYDONIA OBLONGA LEAF

Abstract

In this study, leaf extract of Cydonia oblonga were used to synthesize silver nanoparticles. Silver nanoparticles have been successfully synthesized by environmentally friendly and economical methods. Characterization of synthesized AgNPs used UV-visible spectroscopy (UV-Vis.), thermogravimetric and differential thermal analysis (TGA-DTA), X-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), electron diffraction X-ray (EDX), Fourier transform infrared spectroscopy (FT-IR). XRD results demonstrated that the crystal structure of AgNPs was cubic and had a size of 27.30 nm. AgNPs were found to be spherical in SEM images. The element composition was also revealed by EDX Antimicrobial activity of silver nanoparticles; Gram-negative Escherichia coli was tested on gram positive Staphylococcus aureus and Candida albicans yeast. The concentration values of 0.0552, 0.1535 and 0.0383 mg L-1 were determined as MIC (minimum inhibitory concentration), respectively. It was evaluated that the commercial antibiotics used were more effective at low concentrations compared to colistin, vancomycin and fluconazole.