Beslenme ve Diyetetik Bölümü Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/223

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Assessment Of The Antimicrobial, Antioxidant And Cytotoxic Activities Of The Wild Edible Mushroom Pleurotus Eryngii From Hakkari, Turkey
(2017) Acay, Hilal; Dündar, Abdurrahman; Keskin, Cumali; Kaçar, Semra
Abstract: Mushroom is an important source of natural compounds with acknowledged bioactivity. Pleurotus eryngii, in particular, is widely recognized for its organoleptic quality and favorable healthy effects, being commercially produced in great extent. The present study is just for evaluating the antioxidant capacity (scavenging DPPH radicals, reducing power, Total antioxidant activity by β-carotene-linoleic acid metod, Chelating effect on ferrous ions, superoxide anion radical scavenging activity ), antimicrobial effects (by using the disc diffusion method) and insufficiently studied cytotoxic activity (using PC-3 cell lines) of Pleurotus eryngii by using various solvent systems. For this reason; obtained extracts of the mushroom applying hexane, ethylacetate and methanol respectively were tested with in its biologic activities. As a result, The highest total antioxidant activity by β -carotene-linoleic acid metod was identified in methanol extract (63.62 mg /ml). The highest DPPH scavenging activity was obtained from ethyl acetate extract with 88.81 mg/ml. EDTA is used as standard in methal chelating activity. The highest metal chelating activity of Pleurotus eryngii identified in hexane (88,1 mg /ml) and ethylacetate (88.84 mg /ml) extracts. Additionally, in most of studied tests antioxidant activity of the mushroom increased with increasing concentration. On the other hand, the highest antimicrobial activity was obtained against Pseudomonas aeruginosa ATCC 27853 (11 mm) in ethyl acetate extract. However, the best antimicrobial activities were observed in hexane and methanol extracts for other tested microorganism. It was not determined any antimicrobial activity against Escherichia coli ATCC 25922. It has been found that the potential of cytotoxic activity is depended on concentration and solvent type of extracts. Ethyl acetate extract showed significant inhibitory value at the concentrations of 530 µg/ml (86.20 %) against PC-3 cell lines. Using methanol extract, the maximun inhibition was observed at concentration of 680 µg/ml with value of 51.86 %. Overall, the mushroom used as a daily nutrient could be a source for new drug developments and a kind of treatment in cancer therapies, and also, organic extracts of Pleurotus eryngii may contain substances that stimulate bioactivity
Determination of Antioxidant and cytotoxic activities of king oyster mushroom mediated AgNPs synthesized with environmentally friendly methods
(Medicine Science, 2020) Acay, Hilal; Baran, Mehmet Fırat
Synthesizing nanoparticles (NPs) using wild edible fungi with environmentally friendly synthesis methods is more preferred because of the advantages it provides. The fact that its synthesis is easy, economical, non-toxic and has a wide range of uses increases the interest in this subject. The aim of this study was to determine the antioxidant and cytotoxic activity of biomolecular synthesized Pleurotus eryngii silver nanoparticles (PE-AgNPs) against human prostate carcinoma (PC-3), human cervix (HeLa) and breast carcinoma (MCF-7) cells. PE-AgNPs showed significant cytotoxic activity against HeLa, PC-3, MCF-7 cell lines, and also dimethyl sulfoxide solvents of PE-AgNPs applied for their metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and antioxidant activity using the β-carotene linoleate model system. Biosynthetic PE-AgNPs were found to inhibit the proliferation of PC-3, HeLa and MCF-7 cells with IC50 values of 2.185, 46.594 and 6.169 µg / ml, respectively, during a 24-hour incubation period. With the parallel of increasing concentration (1, 2, 5, 10 mg/mL) the activities were also increased at all the tests studied. At 10 mg/ml antioxidant activities were 82%, 85% and 77% for chelation of ferrous ions reducing power, DPPH scavenging and β-carotene linoleate tests respectively. The results show that PE-AgNPs may contribute to the development of a suitable anticancer drug that can lead to a new development of nanoparticles for cancer treatment. It also appears to be advantageous to use nanotechnology and green chemistry to improve the existing therapeutic properties of P.eryngii.
Determination of fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions of the wild edible mushroom Pleurotus ostreatus and Russula delica with cytotoxic activities on pc-3 cell lines
(2018) Acay, Hilal; Kaçar, Semra; Keskin, Cumali; Dündar, Abdurrahman
Fatty acids (FAs) of Pleurotus ostreatus and Russula delica were identified in TL (Total Lipid), TG (Triacylglycerol ) and PL (phospholipid) fractions. The major FAs of TL, TG, PL in both species were Cl6:0 (palmitic açid, PA), Cl8:1 n-9 (oleic acid, OLA) and Cl8:2 n-6 (linoleic acid, LA). In both species, total PUFA amounts were found to be higher than total MUFA and total SFA in TL, TG and PL fractions. The efficient production of the fatty acids especially linolenic and oleic acids which are majorly needed in building blocks of dietary human has confirmed these species as good source of nutrition. On the other hand, insufficiently studied cytotoxic activity (using pC-3 cell lines) of these mushrooms were investigated by using various solvent systems Ethyl acetate extract of Russula delica and, Pleurltus ostreatus showed significant inhibitory value at the concentrations of 520-530 µg/ml (99,45%- 92,82%) against PC-3 cell lines with IC50; 274,53-297,77 µg /mL respectively. Methanol extracts did not show any cytotoxic activity. lt has been found that the potential of cytotoxic activity is depended on concentration and solvent type of extracts. As a result, the present study is a guide for biochemiçal and nutritional values of the both species and can be useful for further investigation on plıarnıacological applications.
Effect of agro-industrial wastes on production of laccase in submerged cultures of some wild mushrooms.
(2012) Acay, Hilal; Keskin, Cumali; Kaçar, Semra
The lignocellulolytic process includes the degradation of lignin by different types of specialized peroxidase. These activities are controlled by gene families whose members or number varies among different species. Many researchers interested in increasing the production of enzymes produced by white rot fungi which is an imıportant in biotechnology. Indeed, the efficient production of these enzymes, seems to be attractive for biotechnological applications due to their low cost. ln this study, laccase activities of different fungal species which grow in the region of Diyarbakir Mardin naturally (Coriolus versicolor 1, C. versicolor 2, Agrocybe aegerita 1, A. aegerita 2, Armillariella tabescens 1, A. tabesecens 2, Fomes fomentarius 1, F. fomentarius 2, Pleurotus ostreatus) were investigated in agitated submerged culturies media with evaluation of lignocellulose-containing substrates. Whey (PAS) and Saboroud dextrose broth (SDB) were used as culture medium, cotton stalk (P) was also used as an enzyme inducer. In this study, all macrofungus were observed laccase activity. The highest laccase activity has been identifed in A. tabescens 1 (150.47 U/ml) SDB+P medium, A. tabescens 2 (100.24 U/ml) PAS+P medium and A. tabescens 2 (83.85 U/ml) SDB+P medium respectively. As a result of this study, it was clearly seen that the enzyme activity induced by cotton stalk. These species and cotton stalks should be evaluated on biotechnological applications.
Evaluation and characterization of Pleurotus eryngii extract-loaded chitosan nanoparticles as antimicrobial agents against some human pathogens
(Taylor & Francis Online, 2020) Acay, Hilal; Yıldırım, Ayfer; Erdem Güzel, Elif; Kaya, Nalan; Baran, Mehmet Fırat
With the increase of antibiotic resistance, which is present at a worrying rate, research on the use of newly developed nanoparticles as an antimicrobial agent with green biotechnology has intensified. The study aimed to investigate the antimicrobial effects of chitosan nanoparticles (CSNP) synthesized using Pleurotus eryngii extract (PE). Characterization of P. eryngii-loaded chitosan nanoparticles (PE-CSNPs) was performed with Fourier transform infrared spectrophotometer, X-ray diffraction, Field-emission scanning electron microscopy, Brunauer-Emmett-Teller, Differential scanning calorimetry, and zeta potential techniques. The FE-SEM images showed that the surface morphology of nanoparticles is similar to CS, but has more porosity network and smaller dimensions structure. The average particle size of spherical PE-CSNPs was obtained as 330.1 nm. The specific surface area and average pore diameter of the synthesized nanoparticles were found as 3.99 m2g-1 and 2.25 nm, respectively. X-ray diffraction determines the presence of an amorphous peak at 2θ = 21.2° results from CS and PE. PE-CSNPs synthesized using P. eryngii extract showed strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans as 0.0156, 0.0625, 0.0625 and 0.0312 mg ml-1, respectively. Thus, it was determined that chitosan nanoparticles formed by the green synthesis of P. eryngii extract showed strong anti-microbial properties.
Fatty acid compositions of total lipid, phospholipid and triacylglycerol fractions of the wild edible mushroom pleurotus ostreatus and russula delica with cytotoxic activities on prostate carcinoma cell lines
(2019) Acay, Hilal; Baran, Mehmet Fırat
The aim of the study to investigate fatty acids (FAs) of Pleurotus ostreatus and Russula delica in Total Lipid (TL), Triacylglycerol (TG) and Phospholipid (PL) fractions. The major FAs of TL, TG, PL in both species were palmitic acid (PA), oleic acid (OLA), and linoleic acid (LA). In both species, total PUFA amounts were found to be higher than total monounsaturated fatty acids (MUFA) and total saturated fatty acids (SFA) in TL, TG and PL fractions. Also, insufficiently studied cytotoxic activity (using prostate carcinoma (PC-3) cell lines) of these mushrooms were investigated by using various solvent systems. Ethyl acetate extract of Pleurotus ostreatus and Russula delica showed significant inhibitory value at the concentrations of 520-530 μg/ml (99.45-92.82%) against PC-3 cell lines with the half-maximal inhibitory concentration (IC50); 274.53-297.77 μg/mL respectively. The present study is a guide for biochemical and nutritional values of both species and can be useful for further investigation on pharmacological applications.
The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes
(Journal of Planar Chromatography - Modern TLC, 2020) Acay, Hilal; Uzan, Serhat; Baran, Mehmet Fırat; Bilden, Alican; Aygün, Hüsamettin
It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC50 value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver–Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.
Investigation of Nutritional Content, Antioxidant Anticancer and Antimicrobial Activities of Pleurotus eryngii (DC. Ex Fr.) Quel, Extract Obtained by Different Solvents
(KSÜ Tarım ve Doğa Dergisi, 2021) Acay, Hilal; Dündar, Abdurrahman; Kaçar, Semra; Baran, Mehmet Fırat; Keskin, Cumali
The present study related the nutritional contents, fatty acidcompositions, in vitro antioxidant, anticancer and antimicrobialactivities of different solvents extracts of Pleurotus eryngii. Thenutritional contents of the P. eryngii were investigated in detail.Crude protein (g 100 kg-1), carbohydrate (g 100 kg-1), lipid (g 100 kg1), ash (g 100 kg-1), dietary fiber (g 100 kg-1) and energy value (cal100 g-1) of fruiting bodies were analysed. Fatty acid compositions oftotal lipid, phospholipid and triacylglycerol fractions of Pleurotuseryngii were investigated by Gas chromatography. Totally 13 differentfatty acids constituents were identified. Antioxidant propertiesincluding total antioxidant activity, free radical scavenging activity,reducing power activity, metal chelating activity, and superoxideanion radical scavenging activity were evaluated using different tests.The highest total antioxidant, DPPH scavenging, metal chelating, andsuperoxide anion radical scavenging activities were determined inethyl acetate extracts. The potential of cytotoxic activity on PC-3(prostate cancer) cell lines was found to depend on the concentrationand the type of the tested extracts fractions. It was determined thathexane, ethyl acetate, and methanol extracts of Pleurotus eryngii haveinhibition effect on the growth of tested microorganisms. The presentresults suggested that used as a daily nutrient, the Pleurotus eryngiicould be a novel source for new drug developments and promising insome cancer treatment.
Mardin, Türkiye’de doğal olarak yetişen yenilebilen yabani mantar Morchella conica’nın besinsel kalitesi ve biyoaktif özelliklerinin değerlendirilmesi.
(2017) Acay, Hilal; Keskin. Cumali; Kaçar. Semra
Özet: Türkiye zengin ve yenilebilen makrofungus floraya sahiptir. Bu çalışma, Mardinde doğal olarak yetişen Morchella conica’nın kimyasal kompozisyonu, yağ asitleri, aminoasitleri ve biyoaktif özellikleri içeren parametreleri belirlemeyi amaçlamaktadır. Protein, karbonhidrat, yağ, kül, diyet, lif ve enerji içerikleri sırasıyla 20, 64 g/100g.dw, 75.1 g/100g, 3.25/100g, 12.4 g/100g, 30.4g/100 g ve 4257kcal/100g bulunmuştur. Linoleik, oleik ve palmitik asit gibi yağ asitleri oldukça fazlaydı. Temel amino asitler arasında en yüksek valindi ve onu threonin takip etti. β-karoten- linoleic asit metoduyla en yüksek total antioksidan aktivite metanol ekstraktında belirlendi (67.21 mg/ml). En yüksek DPPH radikalini giderme aktivitesi 89.22 mg/ml ile hekzan ekstraktında elde edildi. Diğer taraftan, en yüksek antimikrobiyal aktivite Pseudomonas aeruginosa ATCC 27853’a karşı (10mm) hekzan eksraktında elde edildi. Sitotoksik aktivitenin potansiyelinin konsantrasyon ve ekstraktların solvent tipine bağımlı olduğu bulunmuştur. Etil asetat ekstraktı 550 µg/ml (99.82%) konsantrasyon değerinde (99.82%) PC-3 hücre hattına karşı IC50 264,33 (µg/ml) ile önemli inhibisyon etkisi göstermiştir. Sonuçta, günlük besin olarak kullanılan bu mantar yeni ilaç geliştirmek için ve kanser terapisinde bir çeşit tedavi kaynağı olabilir, ve ayrıca Morchella conica’nın organik ekstartları biyoaktiviteyi teşvik eden maddeler içerebilir.
Morchella esculenta-based chitosan bionanocomposites: Evaluation as an antifungal agent
(Wiley Online Library, 2022) Acay, Hilal; Yildirim, Ayfer; Güney, İnci Güler; Derviş, Sibel
Considering the damage caused by fungicides to human health and problems such as microbial resistance, biological control against plant pathogens has started to gain importance worldwide. This research demonstrates a new, simple, cost-effective, and environmentally friendly method for synthesizing chitosan bionanocomposite (CBNC) from Morchella esculenta (L) Pers-extract (MEE). The antifungal property of the synthesized Morchella esculenta (L) Pers-chitosan bionanocomposite (MCBNC) against some plant pathogens was also evaluated. FTIR, XRD, FE-SEM, DSC, TGA, and BET were used to characterize the synthesized MCBNC. Mushroom-based chitosan nanoparticles were evaluated for antifungal activity against some fungal pathogens, including Neoscytalidium novaehollandiae, N. dimidiatum, Alternaria alternata, Verticillium dahliae, Bipolaris sorokiniana, and Colletotrichum sp. The findings obtained clearly showed that chitosan nanoparticles have antifungal activity. The results suggest that the chitosan nanoparticle can be used in the field to protect various crops from phytopathogens. Novelty impact statement Chitosan bionanocomposite (MCBNC) synthesis was performed for the first time using the wild mushroom Morchella esculenta, which has strong bioactive properties. It was observed that the bionanomaterial, which was characterized by FTIR, XRD, FE-SEM, DSC, TGA, and BET analyses, has high antifungal activity against plant pathogens such as Alternaria alternata and Bipolaris sorokiniana. MCBNCs synthesized by the green synthesis method can be an important area of use in the fight against plant pathogens, which corresponds to 1/3 of the world's agricultural production potential.
Utilization of edible mushroom for nanomaterial-based bioactive material development
(Iranian Journal of Materials Science and Engineering, 2021) Acay, Hilal; Yıldırım, Ayfer; Baran, Ayşe
Gold nanoparticles (AuNP) were synthesized using edible mushroom Russula delica (RD) in this study. Possibilities to evaluate these synthesized nanoparticles (RD-AuNPs) as bioactive substances were investigated. Characterization of synthesized RD-AuNPs were characterized via UV-vis, XRD, FTIR, EDX. In a spherical view, RD-AuNPs with a crystal size of 34.76 nm were synthesized. As a result, fungal systems used for nanomaterial biosynthesis as an effective alternative to chemical synthesis can be used in different biotechnological and medical applications. RD-AuNPs produced by green synthesis can be evaluated in this context.
Utilization of Morchella esculenta-mediated green synthesis golden nanoparticles in biomedicine applications
(Preparative Biochemistry and Biotechnology, 2021) Acay, Hilal
This study aimed to synthesize gold nanoparticles (AuNPs) by hot water extract in room conditions using edible Morchella esculenta (ME) and investigate the bioactive properties of the synthesized Morchella esculenta-based gold nanoparticles (ME-AuNPs). The characterization of the biologically synthesized ME-AuNPs was made using the ultraviolet–visible spectrophotometry, X-ray crystallography, scanning electron microscopy, Fourier transforms infrared spectroscopy, and energy dispersive X-ray spectrum methods. The ME-AuNPs, with a particle size of 16.51 nm, were found to have strong bioactive properties. The antioxidant activity of the ME-AuNPs attempted by metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and the β-carotene linoleate model system. The activities at 10 mg/mL were 82, 85, and 77% for the chelation of ferrous ions, DPPH scavenging, and β-carotene linoleate tests, respectively. The ME-AuNPs also showed strong antimicrobial activity against various pathogen microorganisms and strong cytotoxic activity in the A549 and HepG2 cell lines. This study demonstrated the possibility of using a cheap and nontoxic fungal extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, and biocompatible AuNPs that could be used in future diagnostic and therapeutic applications.
Utilization of Morchella esculenta-mediated green synthesis golden nanoparticles in biomedicine applications
(Taylor & Francis Online, 2021) Acay, Hilal
This study aimed to synthesize gold nanoparticles (AuNPs) by hot water extract in room conditions using edible Morchella esculenta (ME) and investigate the bioactive properties of the synthesized Morchella esculenta-based gold nanoparticles (ME-AuNPs). The characterization of the biologically synthesized ME-AuNPs was made using the ultraviolet-visible spectrophotometry, X-ray crystallography, scanning electron microscopy, Fourier transforms infrared spectroscopy, and energy dispersive X-ray spectrum methods. The ME-AuNPs, with a particle size of 16.51 nm, were found to have strong bioactive properties. The antioxidant activity of the ME-AuNPs attempted by metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and the β-carotene linoleate model system. The activities at 10 mg/mL were 82, 85, and 77% for the chelation of ferrous ions, DPPH scavenging, and β-carotene linoleate tests, respectively. The ME-AuNPs also showed strong antimicrobial activity against various pathogen microorganisms and strong cytotoxic activity in the A549 and HepG2 cell lines. This study demonstrated the possibility of using a cheap and nontoxic fungal extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, and biocompatible AuNPs that could be used in future diagnostic and therapeutic applications.
Yenilebilen Yabani Mantar Morchella esculenta (L.) Pers.’nın Besinsel Kalitesi ve Biyoaktif Özelliklerinin Değerlendirilmesi
(Selçuk Üniversitesi, 2018) Acay, Hilal
Türkiye zengin ve yenilebilen bir makrofungus çeşitlilğine sahiptir. Bu çalışma, Mardin’de doğal olarak yetişen Morchella esculenta (L.) Pers.’nın kimyasal kompozisyonu, yağ asitleri, aminoasitleri ve biyoaktif özellikleri içeren parametreleri belirlemeyi amaçlamaktadır. Protein, karbonhidrat, yağ, kül, diyet lif ve enerji içerikleri sırasıyla 20.64 g/100 g·dw, 75.1g/100 g, 3.25/100 g, 12.4 g/100 g, 30.4 g/100 g ve 4257 kcal/100 g, bulunmuştur. Linoleik, oleik ve palmitik asit gibi yağ asitleri oldukça fazlaydı. Temel amino asitler arasında en yüksek valindi ve onu threonin takip etti. β-karoten-linoleic asit metoduyla en yüksek total antioksidan aktivite metanol ekstraktında belirlendi (67.21 mg/ml). En yüksek DPPH radikalini giderme aktivitesi 89.22 mg/ml ile hekzan ektraktında elde edildi. Diğer taraftan, en yüksek antimikrobiyal aktivite Pseudomonas aeruginosa ATCC 27853 ‘a karşı (10 mm) hekzan ekstraktında elde edildi. Sitotoksik aktivitenin potansiyelinin konsantrasyon ve eksraktların solvent tipine bağımlı olduğu bulunmuştur. Etil asetat eksraktı 550 μg/ml (99.82 %) konsantrasyon değerinde (99.82 %) PC-3 hücre hattına karşı IC50 264.33 (μg/ml ) ile önemli inhibisyon etkisi göstermiştir. Sonuçta, günlük besin olarak kullanılan bu mantar yeni ilaç geliştirmek için ve kanser terapisinde bir çeşit tedavi kaynağı olabilir, ve ayrıca, M. esculenta organik ekstrakları biyoaktiviteyi teşvik eden maddeler içerebilir.

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