Effect of agro-industrial wastes on production of laccase in submerged cultures of some wild mushrooms.

Abstract

The lignocellulolytic process includes the degradation of lignin by different types of specialized peroxidase. These activities are controlled by gene families whose members or number varies among different species. Many researchers interested in increasing the production of enzymes produced by white rot fungi which is an imıportant in biotechnology. Indeed, the efficient production of these enzymes, seems to be attractive for biotechnological applications due to their low cost. ln this study, laccase activities of different fungal species which grow in the region of Diyarbakir Mardin naturally (Coriolus versicolor 1, C. versicolor 2, Agrocybe aegerita 1, A. aegerita 2, Armillariella tabescens 1, A. tabesecens 2, Fomes fomentarius 1, F. fomentarius 2, Pleurotus ostreatus) were investigated in agitated submerged culturies media with evaluation of lignocellulose-containing substrates. Whey (PAS) and Saboroud dextrose broth (SDB) were used as culture medium, cotton stalk (P) was also used as an enzyme inducer. In this study, all macrofungus were observed laccase activity. The highest laccase activity has been identifed in A. tabescens 1 (150.47 U/ml) SDB+P medium, A. tabescens 2 (100.24 U/ml) PAS+P medium and A. tabescens 2 (83.85 U/ml) SDB+P medium respectively. As a result of this study, it was clearly seen that the enzyme activity induced by cotton stalk. These species and cotton stalks should be evaluated on biotechnological applications.