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Citation - WoS: 73
Citation - Scopus: 85
Biosynthesis, characterization, and investigation of antimicrobial and cytotoxic activities of silver nanoparticles using Solanum tuberosum peel aqueous extract
(Elsevier, 2023) Xu, Jiujun; Yıldıztekin, Mahmut; Han, Dayong; Keskin, Cumali; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Aytuğ Ava, Canan; İrtegün Kandemir, Sevgi; Cebe, Deniz Barış; Dağ, Beşir; Beilerli, Aferin; Khalilov, Rovshan
Metallic nanoparticle biosynthesis is thought to offer opportunities for a wide range of biological uses. The green process of turning biological waste into utilizable products gaining attention due to its economical and eco-friendly approach in recent years. This study reported the ability of Solanum tuberosum (ST) peel extract to the green synthesis of non-toxic, stable, small-sized silver nanoparticles without any toxic reducing agent utilizing the phytochemical components present in its structure. UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, flourier scanning electron microscopy, atomic force microscopy, transmission electron microscopy, and energy dispersive analysis X-ray confirmed the biosynthesis and char-acterization of silver nanoparticles. Also, dynamic light scattering and thermogravimetric ana-lyses showed stable synthesized nanoparticles. The antibacterial activity of the biosynthesized silver nanoparticles was evaluated against four different bacterial strains, Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) Bacillus subtilis (B. subtilis), and a yeast, Candida albicans (C. albicans) using the minimum inhibitory concen-tration technique. The cytotoxic activities were determined against Human dermal fibroblast (HDF), glioblastoma (U118), colorectal adenocarcinoma (CaCo-2), and human ovarian (Skov-3) cell lines cancer cells using MTT test. The nanoparticle capping agents that could be involved in the reduction of silver ions to Ag NPs and their stabilization was identified using FTIR. Nano -particles were spherical in shape and had a size ranging from 3.91 to 27.07 nm, showed crys-talline nature, good stability (-31.3 mV), and the presence of capping agents. ST-Ag NPs significantly decreased the growth of bacterial strains after treatment. The in vitro analysis showed that the ST-Ag NPs demonstrated dose-dependent cytotoxicity against cell lines. Based on the data, it is feasible to infer that biogenic Ag NPs were capped with functional groups and demonstrated considerable potential as antibacterial and anticancer agents for biomedical and industrial applications.
Citation - WoS: 22
Citation - Scopus: 24
Boletus edulis loaded with gamma-Fe2O3 nanoparticles as a magnetic sorbent for preconcentration of Co(II) and Sn(II) prior to their determination by ICP-OES
(SPRINGER WIEN, 2018) Özdemir, Sadin; Yalçın, M. Serkan; Kılınç, Ersin; Soylak, Mustafa
The authors show that the fungus Boletus edulis loaded with gamma-Fe2O3 nanoparticles is a viable sorbent for magnetic solid phase extraction of trace levels of Co(II) and Sn(II). The surface structure of immobilized magnetized B. edulis was characterized by FT-IR, SEM and EDX. Experimental parameters were optimized. Following elution with 1 M HCl, the ions were quantified by ICP-OES. The limits of detection are 21 pg.mL(-1) for Co(II), and 19 pg.mL(-1) for Sn(II). The preconcentration factors are 100 for both ions. The sorption capacities of the sorbent are 35.8 mg.g(-1) for Co(II) and 29.6 mg.g(-1) for Sn(II). The method was applied to the analysis of certificated reference materials and gave >= 95% recoveries with low RSDs. It was also successfully applied to the quantification of Co(II) and Sn(II) in spiked environmental and food samples.
Citation - WoS: 29
Citation - Scopus: 34
Comparative and competitive adsorption of gaseous toluene, ethylbenzene, and xylene onto natural cellulose-modified Fe3O4 nanoparticles
(ScienceDirect, 2022) Ece, Mehmet Şakir; Kutluay, Sinan
Many industrial processes produce volatile organic compound (VOC) pollutants within multicomponent systems. Therefore, exploring the comparative and competitive adsorption of VOCs is of both practical and scientific interest. This study elucidates the adsorption behavior of gaseous toluene, ethylbenzene, and xylene (TEX) targeted as VOCs onto natural cellulose-modified Fe3O4 (NC-Fe3O4) nanoparticles (NPs) both individually and in multicomponent systems for the first time in the literature. The characterization of NC-Fe3O4 synthesized via co precipitation method was carried out with analysis techniques including BET, SEM, EDS, FTIR, and TGA-DTA. The adsorption capacities of TEX as a single-component onto NC-Fe3O4 (for 20 mg L-1 TEX inlet concentration) were found as 477, 550, and 578 mg g(-1), respectively. In contrast, with TEX in a binary-component system, the adsorption capacity of the T (for 20 mg L-1 T with 10 mg L-1 E and 10 mg L-1 X, respectively) decreased by approximately 43% and 50% for the binary-mixtures of T-E and T-X, respectively, due to competition with E and X for adsorption sites. Similarly, the adsorption capacity of the E (for 20 mg L-1 E with 10 mg L-1 X) decreased by approximately 46% due to competition with the X for adsorption sites. With TEX in a ternary-component system, the adsorption capacity of the X remained consistent, indicating its competitive dominance over the E and T. The adsorption capacity of NC-Fe3O4 followed the order of X > E > T in the ternary-component system, which agrees with the adsorption results for the single-component system. The adsorption mechanism of TEX was explained by fitting the adsorption data to diverse kinetic and isotherm models. The NC-Fe3O4 with a superior performance in terms of both reuse efficiency and adsorption capacity, could be used as a promising and renewable adsorbent for efficient treatment of VOC pollutants. The findings of the current study will contribute to a better understanding of the comparative and competitive adsorption behaviors among different VOC pollutants in relation to a given adsorbent.
Citation - WoS: 49
Citation - Scopus: 50
Competitive adsorption of VOCs (benzene, xylene and ethylbenzene) with Fe3O4@SiO2-NH@BENZOPHENONE magnetic nanoadsorbents
(Elsevier, 2023) Güngör, Çetin; Ece, Mehmet Şakir
Volatile organic compounds (VOCs), which are toxic, mutagenic and carcinogenic, are considered a critical factor for air pollution and cause serious harm to the eco-environment and human health. In this study, Fe3O4, Fe3O4@SiO2-NH2, Fe3O4@SiO2-NH@BENZOFENONE were synthesized as new magnetic nanoadsorbents (MNAs) and used for the first time in the removal of gas-phase benzene, xylene and ethylbenzene. The synthesised MNAs were characterized by SEM-EDS, TEM, FTIR, XRD, VSM, TGA and BET analyses. The characterization results showed that the MNAs have mesoporous structure, type IV physioresorption and type H3 hysteresis loop character. In order to clarify the comparative and competitive adsorption behaviour, the adsorption capacity of Fe3O4@SiO2-NH@BENZOFENONE MNA was found to be in the order of xylene > ethylbenzene > benzene in both single, binary and ternary component systems. The adsorption kinetics of benzene, xylene and ethylbenzene with Fe3O4@SiO2-NH@BENZOFENONE MNA were found to be governed by multistep mechanisms. Fe3O4@SiO2-NH@BENZOFENONE MNA showed reuse efficiencies of 83.07%, 84.35% and 82.99% after 5 cycles for benzene, xylene and ethylbenzene respectively. In the framework of the results, Fe3O4@SiO2-NH@BENZOPHENONE MNA, which has a high potential in terms of both adsorption capacity and reuse efficiency, is proposed as a promising adsorbent for the efficient removal of benzene, xylene and ethylbenzene. © 2023 Elsevier B.V.
Citation - WoS: 7
Citation - Scopus: 10
Determination of trace Bi by ICP-OES after magnetic solid phase extraction with fullerene C-60 modified gamma-Fe2O3 superparamagnetic iron oxide nanoparticles
(ROYAL SOC CHEMISTRY, 2015) Kılınç, Ersin
In this research, a procedure for the preconcentration of Bi at trace levels in milk samples prior to inductively coupled plasma optical emission spectrometry (ICP-OES) is proposed. It is based on the batch magnetic solid phase extraction of Bi ions by fullerene C-60 modified maghemite (gamma-Fe2O3 superparamagnetic iron oxide nanoparticles - SPION) nanoparticles. The various factors that influence the preconcentration of Bi such as pH, the amount of magnetic nanoparticles, initial Bi concentration, sample volume and the effects of possible interfering ions were investigated. A linear analytical curve was achieved in the concentration range of 0.25-10 ng mL(-1) with a limit of detection of 0.06 ng mL(-1). The precision expressed as a RSD was 7.5% for a concentration of 0.25 ng mL(-1). The method was validated by the analysis of a standard reference material (NIST SRM 1643e). In addition, 96.6-98.7% recoveries were achieved for the spiked samples. Bi concentrations in ten milk samples were successfully determined after the developed method was applied. Bi content in the milk samples ranged from 6.5 to 14.3 ng mL(-1).
Citation - WoS: 67
Citation - Scopus: 73
Ecofriendly Synthesis of Silver Nanoparticles Using Ananas comosus Fruit Peels: Anticancer and Antimicrobial Activities
(Hindawi, 2021) Baran, Ayşe; Keskin, Cumali; Baran, Mehmet Fırat; Huseynova, Irada; Khalilov, Rovshan; Eftekhari, Aziz; Irtegun-Kandemir, Sevgi; Kavak, Deniz Evrim
Metallic nanoparticles are valuable materials and have a range of uses. Nanoparticles synthesized from plant wastes by environment-friendly methods have attracted the attention of researchers in recent years. Also, the advantages of biological resources and synthesis methods are attracting attention. In this study, silver nanoparticles were synthesized from Ananas comosus fruit peels using ecofriendly method steps. The characterization of the particles obtained was determined by using a UV-visible spectrophotometer (UV-Vis.), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction diffractometer (XRD), Fourier scanning electron microscope (FESEM), and transmission electron microscopy (TEM). The nanoparticles showed maximum absorbance at 463 nm, measuring 11.61 in crystal nanosize, and presented spherical in appearance. An antimicrobial activity test was determined with the minimum inhibition concentration (MIC) method. The nanoparticles showed promising inhibitory activity on the Gram-positive and Gram-negative pathogen microorganisms (Escherichia coli ATCC25922, Staphylococcus aureus ATCC29213, Bacillus subtilis ATCC11774, Pseudomonas aeruginosa ATCC27833 bacteria, and Candida albicans yeast) at low concentrations. The cytotoxic and growth inhibitory effects of silver nanoparticles on different cancer cell lines were examined via the MTT assay. © 2021 Ayşe Baran et al.
Citation - WoS: 24
Citation - Scopus: 25
Enantioseparation of Mandelic Acid Enantiomers With Magnetic Nano-Sorbent Modified by a Chiral Selector
(Wiley Online Library, 2015) Tarhan; Tural; Tural; Topal;, Tuba; Bilsen; Servet; Giray
In this study,R(+)-α-methylbenzylamine-modified magnetic chiral sorbent wassynthesized and assessed as a new enantioselective solid phase sorbent for separation ofmandelic acid enantiomers from aqueous solutions. The chemical structures and magnetic prop-erties of the new sorbent were characterized by vibrating sample magnetometry, transmissionelectron microscopy, Fourier transform infrared spectroscopy, and dynamic light scattering.The effects of different variables such as the initial concentration of racemic mandelic acid, dos-age of sorbent, and contact time upon sorption characteristics of mandelic acid enantiomers onmagnetic chiral sorbent were investigated. The sorption of mandelic acid enantiomers followeda pseudo-second-order reaction and equilibrium experiments were wellfitted to a Langmuir iso-therm model. The maximum adsorption capacity of racemic mandelic acid on to the magneticchiral sorbent was found to be 405 mg g 1. The magnetic chiral sorbent has a greater affinityfor (S)-(+)-mandelic acid compared to(R)-( )-mandelic acid. The optimum resolution wasachieved with 10 mL 30 mM of racemic mandelic acid and 110 mg of magnetic chiral sorbent.The best percent enantiomeric excess values (up to 64%) were obtained by use of a chiralpakAD-H column.
Citation - WoS: 3
Citation - Scopus: 2
Examination of the effects of kefir on healing factors in a mice burn model infected with E.coli, S.aureus and P.aeruginosa using qRT-PCR
(Elsevier, 2023) Çetik Yıldız, Songül; Demir, Cemil; Ayhancı, Adnan
Burn areas are susceptible to bacterial growth and infections, particularly in cases with lengthy periods of hospital stay. Burn wound healing, which involves various molecular and cellular mechanisms, continues to be a significant problem. Growth factors and cytokines play an active and vital role in wound healing. In the present study, the effects of kefir on wound healing in a 2nd-degree mouse burn model infected with e.coli, s.aureus and p.aeruginosa were investigated in vitro. In order to clarify the effects of kefir in the wound healing process, the macroscopic changes in kefir-applied scar tissue as well as wound depth and width were examined and IL-1α, IL-1β, IL-6, IL-8, IL-10 and TNF-α, VEGF, TGF-β protein levels were determined using the qRT-PCR method. The findings of the present study show that kefir has a positive impact on the factors playing a role in wound healing and accelerates the healing process.
Citation - WoS: 15
Citation - Scopus: 18
Fullerene C-60 functionalized gamma-Fe2O3 magnetic nanoparticle: Synthesis, characterization, and biomedical applications
(TAYLOR & FRANCIS LTD, 2016) Kilinc, Ersin
Hybrid magnetic nanoparticles composed from C-60 fullerene and -Fe2O3 were synthesized by hydrothermal method. XRD, FT-IR, VSM, SEM, and HR-TEM were employed for characterizations. The magnetic saturation value of C-60--Fe2O3 magnetic nanoparticles was 66.5 emu g(- 1). Concentration of Fe in nanoparticles asdetermined by ICP-OES was 40.7% Fe. Particle size of C-60--Fe2O3 magnetic nanoparticles was smaller than 10 nm. Maximum adsorption capacity of C-60--Fe2O3 for flurbiprofen, a non-steroidal anti-inflammatory drug, was calculated from Langmuir isotherm as 142.9 mg g(- 1).
Citation - WoS: 41
Citation - Scopus: 48
gamma-Fe2O3 magnetic nanoparticle functionalized with carboxylated multi walled carbon nanotube for magnetic solid phase extractions and determinations of Sudan dyes and Para Red in food samples
(ELSEVIER SCI LTD, 2018) Kilinc, Ersin; Celik, Kadir Serdar; Bilgetekin, Havin
Hybrid nanostructures composed of gamma-Fe2O3 (maghemite) and carboxylated-multi walled carbon nanotube (cMWCNT) were used for the magnetic solid phase extractions and determination of Sudan I, II, III, IV, Para Red, Sudan Black B and Sudan Red 7B in chili products. High performance liquid chromatography (HPLC) was employed for the measurements. Limit of quantification (LOQ) values were found in the range 0.44-2.82 ng mL(-1) for analytes. The best extraction parameters were determined as pH 8.0, 40 mg of magnetic nanoparticle, 4.0 min of contact time, 0.3 mL desorption by acetonitrile. The samples were dissolved in acetone-dichloromethane-methanol (3: 2: 1, v/v/v) and diluted with acetonitrile-methanol (v/v; 80: 20) before the method was applied. Concentrations of Sudan dyes and Para Red were determined in four samples of chili powder from less than LOQ to 31.21 +/- 1.6 ng g(-1), two samples of chili tomato sauces (lower than LOQ) and two samples of ketchup (lower than LOQ).
Citation - WoS: 35
Citation - Scopus: 39
Highly improved solar cell efficiency of Mn-doped amine groups-functionalized magnetic Fe3O4@SiO2 nanomaterial
(Wiley Online Library, 2021) Kutluay, Sinan; Horoz, Sabit; Şahin, Ömer; Ekinci, Arzu
Herein, magnetic Fe3O4@SiO2 nanomaterial functionalized with amine groups (Fe3O4@SiO2@IPA) doped with manganese (Mn) was prepared, characterized and used for solar cell application. Fe3O4@SiO2@IPA-Mn was prepared via the co-precipitation and sol-gel techniques. Energy-dispersive X-ray (EDX), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) measurements were performed to examine the structure of Fe3O4, Fe3O4@SiO2, Fe3O4@SiO2@IPA and Fe3O4@SiO2@IPA-Mn. General morphology and textural properties of the prepared magnetic nanomaterials were clarified by Brunauer-Emmett-Teller (BET) and scanning electron microscopy (SEM). In addition, Ultraviolet-visible (UV-Vis) spectroscopy and thermal gravimetric analysis (TGA) were used to have a knowledge about the energy band gap and thermal behavior of the prepared magnetic nanomaterials. The energy band gap of Fe3O4@SiO2@IPA with spinel structure was determined as approximately 2.48 eV. It was understood that Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@IPA showed type IV-H3 hysteresis cycle according to IUPAC. From the BET data, it was determined that the specific surface areas of Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@IPA were 60.85, 28.99 and 40.41 m(2)/g, respectively. The pore size distributions of Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@IPA were calculated as 8.55, 1.53 and 1.70 nm, respectively, by the BJH method. Also, it was observed that the dominant pore widths of Fe3O4, Fe3O4@SiO2 and Fe3O4@SiO2@IPA were calculated similar to 5.58, similar to 0.88 and similar to 17.92 nm, respectively, by the DFT method. Au/CuO/Fe3O4@SiO2@IPA-Mn/ZnO/SnO2: F solar cell device was created using existing Fe3O4@SiO2@IPA-Mn as a buffer layer. The power conversion efficiency (%) of Fe3O4@SiO2@IPA-Mn based solar cell device was calculated as 2.054. This finding suggest that Fe3O4@SiO2@IPA-Mn can be used as a promising sensitizer in solar cell technology. Moreover, in this study, the effectiveness of the modification of manganese (one of the transition metals, which is cheap and easily available) with magnetic nanomaterials in the use of solar cell technology was demonstrated for the first time.
Citation - WoS: 14
Citation - Scopus: 16
In situ atom trapping of Bi on W-coated slotted quartz tube flame atomic absorption spectrometry and interference studies
(PERGAMON-ELSEVIER SCIENCE LTD, 2013) Kilinc, Ersin; Bakirdere, Sezgin; Aydin, Firat; Ataman, O. Yavuz
Analytical performances of metal coated slotted quartz tube flame atomic absorption spectrometry (SQT-FAAS) and slotted quartz tube in situ atom trapping flame atomic absorption spectrometry (SQT-AT-FAAS) systems were evaluated for determination of Bi. Non-volatile elements such as Mo, Zr, W and Ta were tried as coating materials. It was observed that W-coated SQT gave the best sensitivity for the determination of Bi for SQT-FAAS and SQT-AT-FMS. The parameters for W-coated SQT-FAAS and W-coated SQT-AT-FAAS were optimized. Sensitivity of FAAS for Bi was improved as 4.0 fold by W-coated SQT-FAAS while 613 fold enhancement in sensitivity was achieved by W-coated SQT-AT-FAAS using 5.0 min trapping with respect to conventional FAAS. MIBK was selected as organic solvent for the re-atomization of Bi from the trapping surface. Limit of detection values for W-coated SQT-FAAS and W-coated SQT-AT-FAAS was obtained as 0.14 mu g mL(-1) and 0.51 ng mL(-1), respectively. Linear calibration plot was obtained in the range of 2.5-25.0 ng mL(-1) for W-coated SQT-AT-FAAS. Accuracy of the W-coated SQT-AT-FAAS system was checked by analyzing a standard reference material, NIST 1643e. (C) 2013 Elsevier B.V. All rights reserved.
Citation - WoS: 104
Citation - Scopus: 115
Investigation of Antimicrobial and Cytotoxic Properties and Specification of Silver Nanoparticles (AgNPs) Derived From Cicer arietinum L. Green Leaf Extract
(FRONTIERS, 2022) Baran, Ayşe; Baran, Mehmet Fırat; Keskin, Cumali; Hatipoğlu, Abdulkerim
Using biological materials to synthesize metallic nanoparticles has become a frequently preferred method by researchers. This synthesis method is both fast and inexpensive. In this study, an aqueous extract obtained from chickpea (Cicer arietinum L.) (CA) leaves was used in order to synthesize silver nanoparticles (AgNPs). For specification of the synthesized AgNPs, UV-vis spectrophotometer, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), electron dispersive X-ray (EDX), and zeta potential (ZP) analyses data were used. Biologically synthesized AgNPs demonstrated a maximum surface plasmon resonance of 417.47 nm after 3 h. With the powder XRD model, the mean crystallite dimension of nanoparticles was determined as 12.17 mm with a cubic structure. According to the TEM results, the dimensions of the obtained silver nanoparticles were found to be 6.11–9.66 nm. The ZP of the electric charge on the surface of AgNPs was measured as −19.6 mV. The inhibition effect of AgNPs on food pathogen strains and yeast was determined with the minimum inhibition concentration (MIC) method. AgNPs demonstrated highly effective inhibition at low concentrations especially against the growth of B. subtilis (0.0625) and S. aureus (0.125) strains. The cytotoxic effects of silver nanoparticles on cancerous cell lines (CaCo-2, U118, Sk-ov-3) and healthy cell lines (HDF) were revealed. Despite the increase of AgNPs used against cancerous and healthy cell lines, no significant decrease in the percentage of viability was detected. Copyright © 2022 Baran, Fırat Baran, Keskin, Hatipoğlu, Yavuz, İrtegün Kandemir, Adican, Khalilov, Mammadova, Ahmadian, Rosić, Selakovic and Eftekhari.
Citation - WoS: 4
Citation - Scopus: 4
Investigation of serum ischemic-modified albumin, galectin-3, paraoxonase-1, and myeloperoxidase activity levels in patients with acute brucellosis
(Taylor & Francis, 2023) Dündar, Ahmet
Objectives: Infection remains current as an important discussion topic in the etiological factors of atherosclerosis. Ischemic-modified albumin (IMA), galectin-3 (gal-3), paraoxonase-1 (PON-1), and myeloperoxidase (MPO) are biomolecules that play an important role in the pathogenesis of atherosclerosis. Our aim is to investigate serum IMA, gal-3, PON-1, and MPO activity in acute brucellosis infection. Materials and methods: Forty patients with acute brucellosis and 40 healthy individuals were included in the study. Serum IMA, gal-3, PON-1, and MPO activity were analyzed by the ELISA method. Results: In acute brucellosis infection, serum gal-3, IMA, and MPO activities were found to be significantly increased compared to the control group, and PON-1 activity was found to be significantly decreased compared to the control group (p < 0.001). There was a positive correlation between serum IMA, and MPO activity (r = 0.707 p = 0.000) and a negative correlation (r = -0.943, p = 0.000) between PON-1 activity. There was a positive correlation between serum gal-3 and MPO activity (r = 0.683, p = 0.000) and IMA level (r = 0.927, p = 0.000) and a negative correlation between PON-1 activity (r = -0.951, p = 0.000).Conclusion, it was found that serum gal-3, IMA levels and MPO activity increased, while PON-1 activity decreased. These results showed that the oxidant-anti-oxidant balance is impaired in acute brucellosis infection. In addition, these results indicate that brucella infection may be increase the risk of atherosclerosis. Further studies are needed to support our findings.
Citation - WoS: 55
Citation - Scopus: 61
Investigation of the antibiotic resistance and biofilm-forming ability of Staphylococcus aureus from subclinical bovine mastitis cases
(ELSEVIER SCIENCE INC, 2016) Aslantas, Ozkan; Demir, Cemil
A total of 112 Staphylococcus aureus isolates obtained from subclinical bovine mastitis cases were examined for antibiotic susceptibility and biofilm-forming ability as well as genes responsible for antibiotic resistance, biofilm-forming ability, and adhesin. Antimicrobial susceptibility of the isolates were determined by disk diffusion method. Biofilm forming ability of the isolates were investigated by Congo red agar method, standard tube method, and microplate method. The genes responsible for antibiotic resistance, biofilm-forming ability, and adhesion were examined by PCR. Five isolates (4.5%) were identified as methicillin-resistant Staph. aureus by antibiotic susceptibility testing and confirmed by mecA detection. The resistance rates to penicillin, ampicillin, tetracycline, erythromycin, trimethoprim-sulfamethoxazole, enrofloxacin, and amoxicillin-clavulanic acid were 45.5, 39.3, 33, 26.8, 5.4, 0.9, and 0.9%, respectively. All isolates were susceptible against vancomycin and gentamicin. The blaZ (100%), tetK (67.6%), and ermA (70%) genes were the most common antibiotic-resistance genes. Using Congo red agar, microplate, and standard tube methods, 70.5, 67, and 62.5% of the isolates were found to be biofilm producers, respectively. The percentage rate of icaA, icaD, and bap genes in Staph. aureus isolates were 86.6, 86.6, and 13.4%, respectively. The adhesion molecules fnbA, can, and clfA were detected in 87 (77.7%), 98 (87.5%), and 75 (70%) isolates, respectively. The results indicated that Staph. aureus from sublinical bovine mastitis cases were mainly resistant to beta-lactams and, to a lesser extent, to tetracycline and erythromycin. Also, biofilm- and adhesion-related genes, which are increasingly accepted as an important virulence factor in the pathogenesis of Staph. aureus infections, were detected at a high rate.
Citation - WoS: 20
Citation - Scopus: 24
Kinetic and isotherm investigation into the removal of heavy metals using a fungal-extract-based bio-nanosorbent
(Environmental Technology and Innovation, 2020) Yıldırım, Ayfer; Baran, Mehmet Fırat; Acay, Hilal
Adsorption is very economical and environmentally friendly method that is commonly accepted as a promising technique for the removal of heavy metals. In this study a fungal-extract-based (FE-CB) bio-nanosorbent was prepared and used as an efficient biosorbent for the removal of heavy metals, namely Cu(II) and Ni(II), from aqueous solutions. FE-CB was characterized by scanning electron microscope, Brunauer–Emmett–Teller surface area and porosity analyzer, Fourier transform infrared, x-ray diffraction, differential scanning calorimeter, thermalgravimetric analysis and zeta potential. The Brunauer–Emmett–Teller surface area, pore volume and average pore diameter of FE-CB were 7.43 m2/g, 0.060 cm3/g, and 2.82 nm, respectively. The adsorbtion properties of FE-CB onto both Cu(II) and Ni(II) were investigated in terms of biosorbent dosage, temperature, initial concentration of Cu(II) and Ni(II) ions, pH and contact time in the batch experiments. The dependence of the biosorption mechanism on pH was revealed and the optimum pH was determined as 6 for Ni(II) and 5 for Cu(II). The Langmuir and Freundlich isothermal models and the kinetic Pseudo-first-order and Pseudo-second-order kinetic models were used to describe the adsorption performance of FE-CB. The activation energy was calculated by pseudo-second-order rate constants. In addition, thermodynamic parameters, standard Gibbs free energy, standard enthalpy and standard entropy were analyzed using the (Van't Hoff equation). The biosorption process was found to be spontaneous, favorable and endothermic.
Citation - WoS: 44
Citation - Scopus: 51
Magnetic solid phase extractions of Co(II) and Hg(II) by using magnetized C-micaceus from water and food samples
(ELSEVIER SCI LTD, 2019) Ozdemir, Sadin; Mohamedsaid, Siham Abdullah; Kilinc, Ersin; Soylak, Mustafa
A new bio-MSPE sorbent based on the use of C. micaceus and gamma-Fe2O3 magnetic nanoparticle was prepared for the preconcentrations of Co(II) and Hg(II). Critical parameters including pH, flow rate, quantity of C. micaceus, quantity of gamma-Fe2O3 magnetic nanoparticle, eluent (type, concentration and volume), sample volume, and foreign ions were examined. Surface structure and variations after interaction with Co(II) and Hg(II) of bio-MSPE sorbent were investigated by FT-IR, SEM, and EDX. The impact of bio-MSPE column reusage was also tested. The biosorption capacities were determined as 24.7 mg g(-1) and 26.2 mg g(-1), respectively for Co(II) and Hg(II). Certified reference materials were utilized to find out the accuracy of the prepared bio-MSPE method. This novel bio-MSPE method was accomplished by being applied to real food and water samples. In particular, it will be possible to make use of C. micaceus as new alternatives, in environmental biotechnology applications.
Citation - WoS: 57
Citation - Scopus: 58
O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme
(ELSEVIER SCIENCE BV, 2018) Acet, Ömür; Baran, Talat; Erdönmez, Demet; Aksoy, Neşe Hayat; Alacabey, İhsan; Menteş, Ayfer; Odabaşı, Mehmet
We synthesized Ni2+-attached O-Carboxymethyl chitosan Schiff base complexes embedded composite cryogels (Ni2+-O-CMCS-CCs) by means of polymerization of gel-forming precursors at subzero temperatures. Prepared affinity cryogel showed excellent adsorption performance for lysozyme selected as model protein to test adsorption parameters, demonstrating an adsorption capacity of 244.6 mg/g (15.3 mg/g for Ni2+ minus O-CMCS-CCs), with fast adsorption equilibrium within 30 min and good reversibility. The performance of Ni2+-O-CMCS-CCs for lysozyme was also evaluated by SDS-PAGE, and a purification efficiency of 86.9% with 89.5% purification yield was determined. The swelling test, FT-IR, and SEM analysis were carried out for the characterization of Ni2+-O-CMCS-CCs. At the end of 35 adsorption-desorption cycles, there was no significant change in the adsorption capacity. (C) 2018 Elsevier B.V. All rights reserved.
Citation - WoS: 58
Citation - Scopus: 60
Preconcentration of metal ions using microbacteria
(SPRINGER WIEN, 2013) Ozdemir, Sadin; Okumus, Veysi; Dundar, Abdurrahman; Kilinc, Ersin
This review (160 refs). covers the current state of the art of microbacteria-based sorbents for preconcentration of metal ions at trace levels. We highlight advantages and major challenges of the techniques and discuss future perspectives of both batch and column-based methods. Particular attention is paid to the preconcentration of metal ions using resin-immobilized microbacteria for solid phase extractions. We also discuss detection methods including UV-vis spectrophotometry, FAAS, ICP-OES and ICP-MS. Analytical figures of merit are compared, and examples are given for the application to a variety of samples including food, beverages, alloys, water, soil, and geological samples.
Citation - WoS: 35
Citation - Scopus: 40
Preconcentrations and determinations of copper, nickel and lead in baby food samples employing Coprinus silvaticus immobilized multi-walled carbon nanotube as solid phase sorbent
(ELSEVIER SCI LTD, 2019) Ozdemir, Sadin; Kilinc, Ersin; Oner, Ebru Toksoy
Preconcentrations of Cu(II), Ni(II) and Pb(II) ions by using Coprinus silvaticus immobilized multiwalled carbon nanotube (MWCNT) were investigated. Effects of important parameters on preconcentration procedure were examined. The best pH values of for Cu(II), Ni(II) and Pb(II) were found to be 6.0, 6.0 and 4.0, respectively. Flow rate of sample solution was 2.0 mL min(-1), while desorption was achieved at 1.0 mL min(-1) flow rate. Preconcentration factors were achieved as 60 for Cu(II), Ni(II) and 70 for Pb(II) (by dividing initial sample volume to final volume). LODs were calculated as 0.014, 0.016 and 0.093 ng mL(-1), respectively for Cu(II), Ni(II) and Pb(II). Accuracy of the method was checked by applying to certified reference samples. Inductively coupled plasma optical emission spectrometer (ICP OES) was employed for measurements of Cu(II), Ni(II) and Pb(II) in digested baby food samples.