Bitkisel ve Hayvansal Üretim Bölümü

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Bitki Gelişimini Teşvik Eden Bazı Mikroorganizmalar
(2009) Küçük, Çiğdem; Güler Güney, İnci
Bitki gelişimini arttıran Rhizobium spp. , Azospirillum spp. ve Glomus spp. gibi mikroorganizmalar hastalık kontrolünde de etkili olmaktadır. Pseudomonas spp. ve Trichoderma spp. gibi biyolojik mücadele etmenlerinin ise bitki gelişimini teşvik ettikleri son yıllarda yapılan çalışmalarda ortaya konmuştur. Tarımsal ürünleri hastalıklara karşı korumak ve gelişimlerini arttırmak için, bu mikroorganizmaların uygun zamanda ve miktarlarda kullanımları sağlanmalıdır. Böylece kimyasal gübrelerin kullanımları sonucu oluşan problemler azaltılabilir. Bu derlemede, biyolojik gübre etmeni olarak kullanılan mikroorganizmaların hem bitki gelişimini teşvik etmeleri hem de biyolojik mücadelede kullanılmaları ile ilgili yapılan çalışmalar özetlenmiştir.
Occurrence of Phytophthora Cryptogea Causing Root and Collar Rot on Sweet Cherry Trees in Diyarbakır Province of Turkey
(2017) Çiftçi, Osman; Derviş, Sibel; Türkölmez, Şahimerdan; Ulubaş Serçe, Çiğdem
Turkey is the world's largest producer of sweet cherry (Prunus avium L.), a member of stone fruits, with approximately 500 thousand tons of fruit produced annually. 24,385 sweet cherry trees are grown in 1,358 da area of Diyarbakır province with 275 t of fruit produced annually. In May 2015, approximately 30% of 500 5-year-old sweet cherry (P. avium cv. Ziraat 0900) trees grafted onto ‘Mahaleb’ in Çüngüs of Diyarbakır province showed wilting, lack of vigor, and dieback, with severely infected trees dying. Reddish necrotic tissues at the base of the trunk often extending to the main roots were observed on those trees. When they uprooted; necrosis on taproots and decay on feeder roots appeared. Tissue samples taken from the margins of crown and root lesions were placed on grated apple corn meal agar amended with P5ARPH. Plates were incubated for 4 days at 20°C in the dark and a Phytophthora species was consistently isolated from the tissues. The morphological features fit the descriptions of Phytophthora cryptogea Pethybr. & Laff. P. cryptogea was pathogenic on 5 to 7 mm × 20 cm diam. shoots detached from a 1-year-old ‘Mahaleb’ cherry (Prunus mahaleb L.) rootstock tree. Genomic DNA was extracted from a representative isolate. The internal transcribed spacer (ITS) region of rDNA was amplified using the ITS6/ITS4 primer pair and sequenced (GenBank Accession No: MF538788). BLAST searches showed a 99 to 100% identity with many P. cryptogea strains AF087475, AY995400, GU111626, GU111624, KP070713, KP070713, KP070715, KP070719, KP070716, KP070721, KP070709 etc. Deposited in NCBI GenBank and Phytophthora-ID databases. The provenance of P. cryptogea in a sweet cherry orchard in Ankara province (Central Anatolia), in a kiwifruit orchard in Bartın province (Black Sea Region), and in a potato field in Erzincan province (Eastern Anatolia Region) was previously reported in Turkey. However, to our knowledge, this is the first report of natural infection of P. cryptogea in a new region, in the Southeastern Anatolia, causing root and collar rot of cherry trees.
Tarımsal yönden önemli fungusların muhafazası ve koleksiyonları
(IKSAD INTERNATIONAL PUBLISHING HOUSE, 2021) Güler Güney, İnci; Derviş, Sibel
Bitki patojenleri ile ilişkili genotipik, fenotipik ve epidemiyolojik verilerin kolayca erişilebilen ve aranabilen bir formatta arşivlenmesi,patojenlerin değişimini ve hareketini izlemeye yardımcı olabilmektedir. Doğada keşfedilmemiş patojen çeşitliliği, patojen kültürlerinin kataloglanmasının önemi daha da artmaktadır. Etkili hastalık kontrolü geliştirmek için patojen genomiklerinin potansiyelinin farkında olmak referans olarak dizilenmiş izolatları ne kadar etkili kullandığımıza da bağlı olmaktadır. ungal kültür koleksiyonlarının amacı, izolatları elde etme zamanındaki koşullarındaki şekliyle, kültürün genetik, fizyolojik veya morfolojik özelliklerinin hiçbirinde değişiklik olmaksızın canlılığını muhafaza ettirmek ve streyni sonraki araştırma, öğretim ve biyoteknolojik süreçler için ana materyal kaynağı olarak korumaktır.
Factors influencing adaptation of innovations in small ruminant production in the TRC3 Region in Turkey
(Emirates Journal of Food and Agriculture, 2021)
This study investigated the socio-economic factors affecting the adoption of innovation in small ruminant production in TRC3 region in Turkey. Stratified random sampling technique was used to select 366 small ruminant farmers from the region. Semi-structured questionnaire was used to collect main data for the study. Data collected were analyzed using descritive statistics, t-tests and multiple linear regression analysis. Results of the study showed that the farmers had a mean age of 45,8 years and 97,0% were male. Traditional production was dominant among the small ruminant producers and as the number of animal increases the income increases (p< 0,01). Out of twelve independent variables, eight of them were statistically significant on multiple linear regression analysis. The F-statistics was statistically significant at 1% level on communication behavior and animal health practices; 5% level on age, breeding reasons, milking way, produced products and fold type. The coefficient of multiple determination (R2) value was estimated to be 0,470 this implies that 47,0% of total variation in the output of small ruminant production was accounted for by the independent variables that were fitted into the model. The main problems of small ruminant production were high feed prices, high deaths of lamps/goat, insufficient use of pastures because of security problems and lack of shepherd due to young people unwillingness to live in rural areas.
Land Suitability Assessment for Pistachio Cultivation Using GIS and Multi‑Criteria Decision‑Making: A Case Study of Mardin, Turkey
(Environ Monit Assess, 2023)
Site selection for pistachio orchards is an important issue for sustainable agricultural policies, crop productivity, agricultural planning, and communities. This study aims to investigate suitable places for pistachio in the Mardin Province (SE Turkey) by considering several variables, such as meteorological data, topographic conditions, economic factors, and soil characteristics, using Geographic Information System (GIS) and Multi-Criteria Decision Analysis. Pistachio farmers, expert opinions, and literature data were used to determine the requirements for pistachio cultivation. Four main assessment criteria (thirteen sub-criteria), sixty value ranges, and fourteen exclusion criteria were determined for the pistachio land suitability assessment. The weighting of the evaluation criteria was calculated using the Analytical Hierarchy Process (AHP). Farmers and experts have stated that meteorological factors are more important than soil, topography, and economic factors. All data were transferred to the GIS environment, and a land suitability map was created using the weighted linear combination method. The results show that Mardin province has very suitable lands for pistachio cultivation. The resulting map determined that the 228,891.59 ha area in Mardin province is very suitable for pistachio. To evaluate the accuracy of the land suitability map generated for pistachio, the Receiver Operating Characteristic (ROC) curve was used. The value of the area under the curve (AUC) was calculated to be 0.806, which indicates that the study is consistent. The created suitability map will be an essential data source for developing sustainable agricultural strategies in the Southeastern Anatolia region.
İNSANSIZ HAVA ARAÇLARININ TARIMSAL FAALİYETLERDE KULLANIMI VE GELECEĞE YÖNELİK BEKLENTİLER
(İKSAD YAYINEVİ, 2023)
İNSANSIZ HAVA ARAÇLARI
Fusarium spp. associated with crown and root rot in Azerbaijan and Kazakhstan
(2022) Bozoğlu, Tuğba; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, Göksel
Little is known about the distribution and prevalence of pathogens of underground parts of winter wheat in Azerbaijan and Kazakhstan. In this study, a detailed survey study was conducted to assess the distribution of pathogen the country to generate information and understand disease dynamics, as well as to test the pathogenicity of the obtained species on a susceptible wheat cultivar.Wheat (Triticum spp.) is a primary source of calories and protein (Shiferaw et al. 2013), grown on 219 million ha and yielding 760.9 million tons (FAOSTAT 2022). Bread wheat (Triticum aestivum L.) and durum wheat (T. durum Desf.) provide a major contribution to the diets of humans and livestock in Azerbaijan and Kazakhstan, with an average annual production of about 1.82 million tons in a planted area of about 0.59 million ha and with 14.3 million tons produced on 12.1 million ha, respectively. Especially, Kazakhstan’s wheat yield (1182.5 ton/ha) falls far short of the global average (3474.4 ton/ha) due to biotic and abiotic stressors.The complex fungal species attacking the crown and root tissues of wheat causes a serious problem, including damping-off, blight, necrosis, and dry rotting of the root, crown, sub-crown, and lower stem tissues, along with wilting and stunting of seedlings and mature wheat plants (Bockus et al. 2010), resulting in significant yield reductions in the major wheat-producing regions of the world (Gonzalez and Trevathan 2000).Template DNA was extracted from 50–100 mg of fungal powder using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The translation elongation factor 1- alpha (EF1-α) gene and the internal transcribed spacer (ITS) region of ribosomal DNA were amplified with EF1/EF2 (O’Donnell et al. 1998) and ITS1/ITS4 (White et al. 1990) primer sets, respectively.
Morphological, pathogenic and molecular characterization of Globisporangium ultimum causing stem and root-rot disease of bean plants grown in Diyarbakır Province of Turkey
(2017) Çiftçi, Osman; Derviş, Sibel; Türkölmez, Şahimerdan; Ulubaş Serçe, Çiğdem
Bean, Phaseolus vulgaris L., is an economic important herbaceous annual legume plant in the family Fabaceae. It is amongst the most widely cultivated legumes of the world for its delicious seeds having high protein content like other legume seeds. In mid-June2016, we observed bean plants belonging to cv. Ayşekadın at near harvest stage in a commercial field located in Hanzo District of Diyarbakır Province (Southeastern Anatolia) with necrotic taproots and few lateral roots. Infected hypocotyls above the soil line and lower stems had light brown lesions, and plants showed symptoms of wilting. Within a month, the incidence of the affected plants grown in this 30 da field reached 50%. Tissue fragments of 1 mm2 were excised from the root and stem lesion of infected plants, dipped in a solution containing 1% sodium hypochlorite, and plated on grated apple corn meal agar (GACMA) amended with P5ARPH. Plates were incubated at 22°C for 5 days. A Pythium-like organism was consistently isolated from tissues. Growing hyphal tips of isolates were transferred onto V8 medium for production of sexual structures. All isolates were identified as Globisporangium ultimum (Syn: Pythium ultimum) based on the morphological characters of sporangia, oogonia, antheridia, oospores and hyphal swellings. To confirm Koch's postulates, two isolates were tested for pathogenicity against bean (cv. Ayşekadın) by placing colonized GACMA plugs or GACMA alone next to the crown. Symptoms similar to those observed in the field on bean developed on inoculated plants and the pathogen was reisolated. Controls did not develop disease. The internal transcribed spacer (ITS) region of rDNA of a single isolate was amplified using the ITS6/ITS4 primer pair and sequenced. BLAST analysis of the ITS sequence (GenBank Accession No MF536533) showed a 100% homology with the corresponding sequences of many isolates of G. ultimum in GenBank and confirmed our identification of this isolate as G. ultimum. Collar and root rot caused by G. ultimum affects bean plants in many regions of the world. The pathogen was also reported in Hatay and Samsun provinces of Turkey. No published information exists, however, on the existence of this pathogen in the Southeastern Anatolia Region (Diyarbakır). Besides, this is first report of molecular characterization of G. ultimum in Turkey.
Rehabilitation of soils containing high salt levels with beneficial fungi
(2023) Türkölmez, Şahimerdan; Un, Akin; Derviş, Sibel; Dikilitaş, Murat
Soil salinity not only reduces the quality of the soil, but also causes ionic imbalance in the plant, competition in the nutrient uptake and toxic effects at high concentration. Combating salinity, which has become a greater stress factor due to impact of drought and high temperatures, is of great importance. In this study, 3 kg soil in pots, adjusted to 12 mS/m EC, under four different treatments (3 different fungi species and control) were compared in a laboratory environment. The experiment was designed with three replications for each subject and one plant in each pot. Fungi species (Clononotachys rosea, Trichoderma sp., Taloromyces funiculosus) were used as soil inoculant to reduce soil salinity. The soil electrical conductivity (EC) was between 9.25 and 9.95 mS/m within 1 month in tomato cultivated soils, while the EC of the plant-free medium was between 10.4 and 11.4 mS/m: thus, the salt content did not differ statistically. EC values of the saline soils was decreased by 21-, 17-, 29 %, respectively, when Trichoderma sp., Taloromyces funiculosus, Clononotachys rosea were applied to tomato plant growing soils. These fungi also decreased the soil EC values by 15-, 16-, 25 %, respectively, in the plant-free environment. The chlorophyll SPAD value of tomato plants grown in saline soils decreased from 22.65 to 0, however, the SPAD values of tomato plants grown in soils with Clononotachys rosea, Trichoderma sp., Taloromyces funiculosus were increased from 22.4-, 25.6-, 24.1 to 29.3-, 50.2-, and 24.5. Similar findings were also observed for the increase in the chlorophyll content of the tomato plant. The fungi mentioned above also contributed to the increase of soil macro (Ca, Mg, K, P) and micro (Fe, Cu, Zn, Mn) element contents, and to the improvement of soil available phosphorus amount (P2O5) with the increase in soil phosphorus content. Fungi inocula induced a statistically significant increase of soil micronutrient concentrations (p>0.05). Sodium (Na) concentration in saline soils did not change in inoculated-non-inoculated soils. These findings were interpreted as fungi inocula were not able to assimilate the salt from the soil, but they instead contributed to the release of macro and micro elements that were unavailable under saline conditions, possibly due to their metabolic activities. According to the results of soil analysis at the end of the experiment, it was revealed that the pH value was 7.72 in the control pots and 3.28 in the pots treated with Taloromyces funiculosus, and the EC value was 12.34 and 9.74 mS/m, respectively. Among the fungi studied, Taloromyces funiculosus was found to be more effective than other two fungi on decreasing soil salinity and increasing micronutrient availability to plants.
Inoculation Techniquesfor Assessing PathogenicityofRhizoctonia solani, Macrophomina phaseolina,Fusarium oxysporumand Fusarium solani on Pepper Seedlings
(2018) Güler Güney, İnci; Güldür, Mehmet Ertuğrul
In this study, surveys were carried out during 2015 and 2016 for wilt and root rot diseases caused by Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, and Fusarium solani in pepper fields in Adıyaman, Diyarbakır, Mardin and Şanlıurfa provinces of Turkey. The purpose of this study was to evaluate the effects of differentinoculation methods (root dip, soil infestation with wheat bran and soil infestation with rice grain) on pathogenicities of R. solani, M. phaseolina,F. oxysporum andF. solani on pepper seedlings. Inoculated pepper seedlings (cv. İnan-3363) were left to grow for three months after transplanting under growth chamber conditions. Inoculation of infective rice-grain was used to test pathogenicity of all four fungi. Root dip inoculation method was used for F. solani and F. oxysporum when the soil was infestedwith wheat branmethod for R. solani andM. phaseolina inoculation. Alltested isolates resultedin thestemand root rot, leaf chlorosis and bruising. To test the pathogenicity of fungi, soil infestation with rice grain inoculation was the most suitable method.All tested fungiinduced similarfoliar symptoms,root rot severityand caused asimilarreduction in dryroot weightswhen rice-grain inoculum wasused. With other inoculation methods, all pathogens similarly affected root rot severity. Whereas, F. oxysporum was the least virulent pathogen among tested fungi affecting foliar symptom severity for fresh root and plant weights, and dry root and plant weights R. solani, M. phaseolina andF. solani weresimilarlyvirulent when these parameters were used. The results of the present study may have a useful connotation to monitor pepper seedlings against these pathogens. In conclusion, we recommend rice-grain inoculation to test pathogenicities of R. solani, M. phaseolina, F. oxysporumandF. solanion various pepper cultivars.
Correction to: Leaf spot caused by Alternaria crassa on Datura stramonium in Turkey
(Australasian Plant Disease Notes, 2022) Bozoğlu, Tuğba; Alkan, Mehtap; Derviş, Sibel; Özer, Göksel
In August 2021, jimson weed (Datura stramonium) plants growing as weeds in potato fields in Bolu province, Turkey, exhibited leaf spots with dark concentric rings. Sunken and lens-shaped lesions with a light center were also frequently observed on petioles, branches, and stems. Based on morphological characteristics and phylogenetic analysis of the transcription elongation factor 1-α, RNA polymerase second largest subunit, and glyceraldehyde-3- phosphate dehydrogenase loci, the causal agent was identified as Alternaria crassa. The pathogen was successfully re-isolated from inoculated jimson weed plants in the pathogenicity assay, proving Koch’s postulates. Alternaria crassa caused necrotic lesions on potato plants, similar to those of early blight, confirming them as an alternative host of the pathogen. This is apparently the first report of leaf spot caused by A. crassa on jimson weed in Turkey.
TIBBİ ve AROMATİK BİTKİ ve UÇUCU YAĞLARININ ANTİFUNGAL AKTİVİTELERİNDE SON GELİŞMELER
(PARADİGMA AKADEMİ YAYINLARI, 2022) Keske, Mehmet Ata; Güler Güney, İnci
Tıbbi ve aromatik bitki ekstraktlarının ve uçucu yağlarının bitki fungal hastalıklarını kontrol etmek için uygulanabilir olması için daha fazla araştırma yapılması gerekmektedir. Ayrıca son çalışmalarda, yeşil nanoteknoloji üzerinde durulmuş, bu tarz çalışmalar önem kazanmıştır (Nematollahi, 2015). Yeşil nanoteknolojide, yeşil bitki ekstraktları olarak tıbbi ve aromatik bitkilerin nanopartikülleri kullanılarak belli başlı patojenlere karşı antimikrobiyal ajan olarak kullanılabilirliği araştırılmıştır. Bu derlemede tıbbi ve aromatik bitki ekstraktlarının ve uçucu yağlarının belli başlı fungal patojenlere (Colletotrichum gloeosporioides Penz. & Sacc., Aspergillus spp., Penicillium spp., Rhizopus spp., Botrytis spp. ve Alternaria spp., Verticillium, Pythium, Rhizoctonia solani Kühn. ve Macrophomina phaseolina (Tassi) Goidanich, Fusarium spp., Sclerotinia spp., Phytophthora spp. vb.) karşı antifungal aktiviteleri incelenmiştir.
ITS and LSU-rDNA nucleotide sequences based confirmation of Cytospora chrysosperma and Chondrostereum purpureum from symptomatic cankered tissues of Populus nigra trees in Turkey
(2017) Derviş, Sibel; Çiftçi, Osman; Türkölmez, Şahimerdan; Ulubaş Serçe, Çiğdem
Malatya ili Doğanşehir ilçesinde 2016 yılında yapılan arazi çalışmaları sırasında gövde, dal kanseri ve kuruma belirtileri gösteren kavak (Populus nigra) ağaçlarından alınan örneklerden yapılan laboratuar çalışmaları sonucunda piknidyum içeren kabukların altından ve odun dokularından sırasıyla Cytospora chrysosperma ve Chondrostereum purpureum izole edilmiştir. İlkbaharda, kavak ağaçlarının sürgünlerine, tamamen gelişmiş olan dördüncü yapraklarının koparılması sonucu ortaya çıkan yaralar üzerine, C. chrysosperma ve C. purpureum izolatları tarafından kolonize edilmiş agar disklerinin yerleştirilmesiyle inokulasyon yapılmıştır. İnokülasyondan üç ay sonra C. chrysosperma ve C. purpureum ile inokulasyon bölgesinde sırasıyla 6,4 ve 3,3 cm uzunluğunda kanserler oluşmuş ve sürgünler büzüşmüştür. Benzer bir şekilde, serada gerçekleştirilen patojenite testlerinde, kabuk dokusunda oluşturulan yaraların bu izolatlar ile inokülasyonundan yaklaşık 14 gün sonra kanser oluşumu gerçekleşmiştir. Hastalanan bitkilerin dokularından yapılan izolasyonlarda C. chrysosperma ve C. purpureum’un tekrar izole edilmesi ile hastalık etmenlerinin bu funguslar olduğu doğrulanmıştır. Steril ortam diskleri ile inokule edilen kontrol sürgünlerdeki yaralarda kanser oluşmamıştır. Her fungal türün temsili izolatından tüm DNA’nın izolasyonu yapılmıştır. İzole edilen toplam DNA’lar, rDNA'nın internal transcribed spacer (ITS) ve large subunit (LSU) gen bölgeleri için sırasıyla ITS6/ITS4 ve NL1/NL4 primer çiftleri kullanılarak amplifiye edilmiş ve dizilenmiştir. BLAST analizleri sonucunda, daha önce Gen Bankası’nda kaydedilen birçok C. chrysosperma ve C. purpureum ITS ve LSU nükleotid dizisi ile %99 benzerlik göstermiştir. Bu diziler Gen Bankasına kaydedilmiştir. C. chrysosperma ve C. purpureum’nın ITS-rDNA için NCBI’dan verilen erişim numaraları sırasıyla MF536529 ve MF536531; LSU-rDNA için veriler erişim numaraları ise sırasıyla MF536530 ve MF536532’dir. Bu fungus etmenlerinin Türkiye'deki varlığı daha önce bildirilmekle birlikte bu çalışma, C. chrysosperma ve C. purpureum'un ITS ve LSU-rDNA nükleotid dizilerine dayanarak moleküler karakterizasyonlarının ilk raporudur.
ITS and LSU-rDNA nucleotide sequences based confirmation of Cytospora chrysosperma and Chondrostereum purpureum from symptomatic cankered tissues of Populus sp. trees in Turkey
(2017) Derviş, Sibel; Çiftçi, Osman; Türkölmez, Şahimerdan; Ulubaş Serçe, Çiğdem
The fungi Cytospora chrysosperma and Chondrostereum purpureum were isolated from orange-brown inner bark with pycnidia in the bark surface and underlying wood tissues of infected poplar plants (Populus sp.) with symptoms of stem and branch canker in Doğanşehir, Malatya, in 2016, respectively. Twigs of poplar trees were inoculated during their first season of growth by removing the fourth fully expanded leaves and placing agar plugs colonized by representative isolates of C. chrysosperma and C. purpureum over the resulting wounds. Three months after inoculation, cankers in 6.4 and 3.3 cm length formed by C. chrysosperma and C. purpureum, respectively, and twigs were girdled. Pathogenicity tests in a greenhouse experiment by shallow wounds made into the bark tissue and inoculation with these isolates in a similar manner also resulted in canker formation in and around inoculated wounds 14 days after inoculation. Subsequent re-isolations of C. chrysosperma and C. purpureum confirmed that these fungi were the causal agents of the disease, and no cankers formed in wounds that received only sterile plugs. DNA was extracted from representative isolates of each fungal species. Extracted DNA templates were amplified and sequenced for rDNA internal transcribed spacer (ITS) and the large subunit (LSU) rDNA gene regions using ITS6/ITS4 and NL1/NL4 primer pairs, respectively. NCBI BLAST results showed 99% similarity with the ITS and LSU sequences of C. chrysosperma and C. purpureum in GenBank. The sequences were submitted to GenBank. Given accession numbers of C. chrysosperma and C. purpureum were MF536529 and MF536531 for ITSrDNA; MF536530 and MF536532 for LSU-rDNA, respectively. Existence of these fungi in Turkey was previously reported. However, this is a first report of molecular characterization of C. chrysosperma and C. purpureum based on ITS and LSU-rDNA nucleotide sequences of these fungi in Turkey.
Morphological and molecular identification of Fusarium spp. causing diseases in strawberry
(2017) Doğaner, Müge; Ayyaz, Mahmood; Derviş, Sibel; Ulubaş Serçe, Çiğdem
Soil-born fungal diseases cause significant losses in strawberry culture and Fusarium is one of the most important among them. The present study was conducted to investigate Fusarium species causing wilting in strawberry plants. Morphological and molecular detection of different Fusarium species related to wilting diseases in strawberries was objective of the study. The fungal isolates were collected from the main strawberry growing areas in Anamur and Silifke districts of Mersin province, Gazipaşa and Aydıncık districts of Antalya province, and Sultanhisar district of Afyon province. Total of 300 samples were analyzed morphologically and 39% of them found infected with Fusarium species. The pathogenicity of six morphologically distinct Fusarium isolates were tested utilizing root dipping to spore suspension method using strawberry variety ‘‘Festival’’. After pathogenicity tests, total DNA from mycelial cultures of six Fusarium isolates were extracted and ribosomal DNA ITS region was amplified using ITS6/4 primers by PCR. The amplicons were sequenced and obtained DNA sequences were blasted in NCBI and Fusarium ID GeneBanks. The sequences of all morphologically identified Fusarium isolates showed 99-100% identity with the Fusarium species deposited in NCBI. Furthermore, the investigated Fusarium species were identified as Fusarium oxysporum, and F. proliferatum according to NCBI but F. incarnatumequiseti species complex and F. concolor according to Fusarium ID.
Bipolaris sorokiniana associated with common root rot in Azerbaijan and Kazakhstan
(2022) Alkan, Mehtap; Bozoğlu, Tuğba; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, Göksel
Wheat (Triticum spp.) is the third most important crop in terms of global production, with an average annual production of almost 219 million ha and yielding 760.9 million tons, after maize and rice. However, A complex of fungi attacking the crown and root tissues of wheat causes a serious problem and significant yield reductions in wheat. Although most prevailing species in this complex can change yearly and regionally in dryland winter wheat production areas, Bipolaris sorokiniana Shoemaker (teleomorph: Cochliobolus sativus) is one of the dominant species and causes spot blotch and common root rot (CRR) on wheat plants, especially in winter wheat varieties. Comprehensive surveys were conducted to identify B. sorokiniana associated with CRR of wheat throughout the main wheat-growing areas of Azerbaijan and Kazakhstan in 2017 and 2019, respectively. As a result of the classification of species based on morphological and molecular tools, 96 and 547 isolates were identified as B. sorokiniana for Azerbaijan and Kazakhstan, respectively. All surveyed regions for these countries were contaminated with this pathogen. Some representative isolates of B. sorokiniana were tested for their ability to cause disease and produced moderate disease severities on cultivar Seri 82 (Triticum aestivum, bread wheat). The percent incidence of B. sorokiniana isolates in all isolates was 15.2% for Azerbaijan, whereas, for Kazakhstan, B. sorokiniana was the most frequently recovered species in the three regions surveyed, with an isolated frequency of 44.80%. The results of the current study provide crucial and helpful information to improve disease management strategies against CRR of wheat in these countries.
Alternaria spp. causing postharvest decay on apple fruit in Nigde province, Turkey
(2022) Tulukoglu Kunt, Keziban Sinem; Derviş, Sibel; Özden, Mustafa
Postharvest rots caused by Alternaria spp. result from infections occurring in the apple orchard at or before harvest and these may remain quiescent during the growing phase and only result in decay during postharvest cold storage. Therefore, the objective of this study was to identify and characterize Alternaria spp. associated with apple black spot and/ or decay during cold storage in Nigde, Turkey. Isolates of Alternaria spp. were obtained from apple fruit (cvs Fuji, Golden Delicious, Granny Smith, Red Delicious, and Starking Delicious) with dark, dry, spongy lesions. The symptomatic fruits were sampled from 18 commercial cold storages in the Nigde Province, Turkey, during the 2020/21 storage period. Decayed fruits were transferred to the laboratory for isolation of the fungi from the lesions. In total, 75 Alternaria isolates were characterized morphologically and were differentiated into 5 phenotypic groups based on their colony morphology, and characteristics of their conidiophores and conidia. At least 5 representative isolates were randomly chosen from each group for the pathogenicity tests. All Alternaria spp. isolates caused round, dry, dark, spongy lesions on/in the inoculated fruits and re-isolates from the inoculated apples of each phenotypic groups were similar to the initial isolates of each group in morphological characteristics. Molecular characterization of isolates is in progress. Since mycotoxin produced by Alternaria spp. in fruits is of unavoidable risk to human health, special care should be given in regards to the need to reduce widespread postharvest losses caused by Alternaria spp. in the cold storages of Niğde, which is the most important apple producer province of the country.
New disease caused by Neoscytalidium dimidiatum devastates tomatoes (Solanum lycopersicum) in Turkey
(2019) Türkölmez, Şahimerdan; Derviş, Sibel; Çiftçi, Osman; Ulubaş Serçe, Çiğdem; Dikilitaş, Murat
A novel disease of tomato (Solanum lycopersicum L.) was observed in the Southeast Anatolia Region of Turkey. Symptoms were blight of all aerial parts of the plant, including stems, branches, leaves, petioles, flowers and fruits, defoliation, root rot, inner stem necrosis, and plant death. The disease was found in 13.9% of surveyed fields, with an incidence varying from 3% to nearly 75% (average 21.2%) of the plants in symptomatic fields. The average severity of blight on stem in fields with the symptomatic plant surveyed was 1.4%. A Botryosphaeriaceae species, identified as Neoscytalidium dimidiatum (Penz.) Crous & Slippers using morphological and cultural features, was consistently isolated from symptomatic roots, inner stems, and blighted leaves, shoots, stems, fruits and flowers. The partial nucleotide sequence data for three gene loci, including nuclear rDNA internal transcribed spacer (ITS), large subunit (LSU) genes and the translation elongation factor 1-alpha (TEF-1α), confirmed the morphological identification. Furthermore, sequence data of actin genes from N. dimidiatum was, for the first time, deposited to the GenBank. Koch's postulates were fulfilled by testing the susceptibility of different tomato tissues (leaves, stems, inner stems and roots of tomato seedlings, and detached tomato fruits and flowers) to N. dimidiatum inoculation. To our knowledge, this is the first report of N. dimidiatum on tomato.
Fungi isolated from cankered tissues of declining apricot trees in Malatya and Elazığ provinces of Turkey
(2017) Türkölmez, Şahimerdan; Çiftçi, Osman; Derviş, Sibel
Surveys were carried out in apricot (Prunus armeniaca L.) production areas of Malatya and Elazığ provinces from April to November in 2015 and 2016. Fungal and oomycetous diseases causing dieback and decline symptoms were investigated and locations where the diseases were prevalent were determined according to the districts in these provinces. Nine and 40 orchards were visited in Elazığ and Malatya during the course of the surveys. A total of 665 out of 5750 apricot trees were checked and the disease incidence was found to be 44% in the surveyed orchards. Out of isolates obtained from root and crown tissues of symptomatic trees, isolates obtained from cankered tissues were characterized according to their morphological characteristics. Genomic DNA was extracted from representative isolates. The internal transcribed spacer (ITS) region of rDNA was amplified using the ITS6/ITS4 primer pair and sequenced and submitted to GenBank. NCBI BLAST results showed 98 to 100% similarity with the ITS sequences of many Clonostachys rosea f. rosea (Link : Fr.) Schroers et. al. 1999 (Ascomycetes, Hypocreales), Sarocladium kiliense (Grütz) Summerb. 2011 (Ascomycetes, Incertae sedis) (Syn: Acremonium kiliense), Phoma sp. (Ascomycetes, Pleosporales), Entoleuca spp. (Ascomycetes, Xylariales) strains deposited in NCBI GenBank. The sequences were submitted to GenBank and given accession numbers were MF536537 and MF536538 for C. rosea, MF536539 for S. kiliense, MF536540 and MF536541 for Phoma spp., and MF536542, MF536543, MF536544 and MF536545 for Entoleuca spp. isolates. Moreover, Verticillium dahliae and Macrophomina phaseolina were also isolated from inner tissues of necrotic branches and morphologically identified. However, pathogenicity of these isolates needs further investigations. If some isolates were not pathogenic, their endophytic or hperparasitic characteristics against pathogenic ones should be tested in order to fully exploit their potential for use as biological control agents.
Root and stem rot caused by Fusarium solani on a new host, apricot
(2017) Türkölmez, Şahimerdan; Çiftçi, Osman; Derviş, Sibel; Ulubaş Serçe, Çiğdem
Apricot plantations (Prunus armeniaca L.) in Malatya and Elazığ cover approximately ten thousand hectares with nearly 10 million trees. In a survey carried out in apricot production areas of Malatya and Elazığ provinces from April to November in 2015 and 2016, apricot trees displayed symptoms of yellowing, stunting, rotting of roots and basal stems, and wilting, especially on those with injuries. A severe brown discoloration of vascular tissue along the stems of infected trees was also observed. Tissues samples collected from symptomatic trees were disinfected with 2% sodium hypochlorite and isolations were conducted on potato dextrose agar (PDA). A Fusarium sp. was consistently isolated from the roots and stems of diseased trees at Pötürge, Doğanşehir, Darende, Doğanyol, Akçadağ, Battalgazi and Baskil districts with 5.7, 10.0, 2.0, 3.3, 6.7, 6.7 and 6.7% incidence, respectively. All isolates obtained had white fluffy aerial hypha on PDA. Morphological characteristics of two types of conidia, macroconidia with three to five septate and microconidia with mostly non-septate to one septate, and chlamydospores produced pointed the fungal isolates to be Fusarium solani (Mart.) Sacc. 1881 (Ascomycetes, Hypocreales). Microconidia were abundant and macroconidia were sparse on PDA. To confirm pathogenicity, 20 healthy 1-year-old wild apricot ‘Zerdali’ rootstock seedlings grown in pots (25 cm in diameter) with sterilized soil were used for two experiments. For the first experiments, a conidial suspension from one isolate (Fs3) cultivated on PDA plates at 28°C for 7 days was used for root inoculation of 6 plants by submerging roots for 20 min in a conidial suspension (5×105 conidia/ml). Four seedlings inoculated with sterile water were used as controls. After 1 month incubation in a greenhouse, dark brown lesions were observed in the inoculated mature roots but not in the control roots. Pathogenicity was also confirmed by stem inoculations of plants in the second experiments. Six plants were inoculated with one mycelium disk of Fs3 (1 cm diameter) each, and sterile PDA disks were placed on four additional plants as controls. The inoculation site was wrapped with Parafilm for 2 days, and then the film was removed. After 1 month, symptoms similar to those observed in the field developed on the trunks of all inoculated plants, while only slight scars formed on the control plants. F. solani was reisolated from all inoculated root and stem tissues. For species confirmation, the internal transcribed spacer region (ITS) of rDNA of Fs3 isolate was amplified using the ITS6/ITS4 primer pair and sequenced. NCBI BLAST results of a 509-bp sequence shared 100% identity with those of many F. solani GenBank accessions previously reported. The new sequence was deposited in GenBank (Accession No. MF536534). To our knowledge, this is the first report of F. solani causing disease on this host plant, P. armeniaca, in Turkey and worldwide, which may help to establish the appropriate measures to control this disease.

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