Bitkisel ve Hayvansal Üretim Bölümü Koleksiyonu
Permanent URI for this collectionhttps://hdl.handle.net/20.500.12514/201
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Browsing Bitkisel ve Hayvansal Üretim Bölümü Koleksiyonu by Department "MAÜ, Meslek Yüksekokulları, Kızıltepe Meslek Yüksekokulu, Organik Tarım Bölümü"
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Conference Object Alternaria spp. causing postharvest decay on apple fruit in Nigde province, Turkey(2022) Derviş, Sibel; Derviş, Sibel; Özden, MustafaPostharvest rots caused by Alternaria spp. result from infections occurring in the apple orchard at or before harvest and these may remain quiescent during the growing phase and only result in decay during postharvest cold storage. Therefore, the objective of this study was to identify and characterize Alternaria spp. associated with apple black spot and/ or decay during cold storage in Nigde, Turkey. Isolates of Alternaria spp. were obtained from apple fruit (cvs Fuji, Golden Delicious, Granny Smith, Red Delicious, and Starking Delicious) with dark, dry, spongy lesions. The symptomatic fruits were sampled from 18 commercial cold storages in the Nigde Province, Turkey, during the 2020/21 storage period. Decayed fruits were transferred to the laboratory for isolation of the fungi from the lesions. In total, 75 Alternaria isolates were characterized morphologically and were differentiated into 5 phenotypic groups based on their colony morphology, and characteristics of their conidiophores and conidia. At least 5 representative isolates were randomly chosen from each group for the pathogenicity tests. All Alternaria spp. isolates caused round, dry, dark, spongy lesions on/in the inoculated fruits and re-isolates from the inoculated apples of each phenotypic groups were similar to the initial isolates of each group in morphological characteristics. Molecular characterization of isolates is in progress. Since mycotoxin produced by Alternaria spp. in fruits is of unavoidable risk to human health, special care should be given in regards to the need to reduce widespread postharvest losses caused by Alternaria spp. in the cold storages of Niğde, which is the most important apple producer province of the country.Conference Object Antifungal effect of boron compounds against Neoscytalidium dimiatum(2023) Derviş, Sibel; Türkkan, Muharrem; Yiğit, Abdurrahman; Derviş, Sibel; Özer, Göksel; Erper, İsmailNeoscytalidium dimidiatum has been recently identified as the agent responsible for canker, dieback, shoot blight, and root rot on different hosts in Türkiye. In this study, we evaluated the inhibitory effect of boric acid (H3BO3), three borates [disodium octaborate tetrahydrate (Na2B8O13.4H2O), disodium tetraborate decahydrate (Na2B4O7.10H2O) and disodium tetraborate (Na2B4O7)] on the mycelial growth, germ tube elongation and conidial germination of N. dimidiatum. The antifungal effects of the boron compounds were investigated at 0.125, 0.25, 0.5, 1.0 and 2.0% (w/v) concentrations. The differences observed between the inhibitory effects of boron compounds on the parameters were found to be significantly important at P < 0.05. The 0.5% concentration of disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited the fungus, whereas other salts did not. Disodium tetraborate decahydrate and disodium octaborate tetrahydrate completely inhibited three parameters at 0.5% and higher concentrations. However, boric acid and disodium tetraborate were able to completely inhibit investigated factors of N. dimidiatum at 2.0% and 1.0% concentration, respectively. Disodium tetraborate and boric acid at 0.5% concentration decreased germ tube elongation and conidia germination of the fungus by 94.97%-63.57%, 59.33%-51.26%, respectively. The effectiveness of the 0.5% concentration on conidial germination was also similar in both salts at P < 0.05. However, disodium tetraborate inhibited germ tube elongation more effectively than boric acid. The minimum inhibition concentration (MIC) values of the four boron compounds also varied between 0.5% and 2.0% concentrations. The boron compounds in this study could be potential agents to manage N. dimidiatum.Conference Object Biber (Capsicum annum L.) Bitkisinden İzole Edilen Endofitik Bakteri ve Topraktan İzole Edilen Bakterilerin Toprak Kökenli Fusarium solani, F. oxysporum, Macrophomina phaseolina ve Rhizoctonia solani’xxye Karşı Antagonistik Aktivitelerinin Belirlenmesi(2017) Güler Güney, İnci; Güldür, Mehmet ErtuğrulForty-nine endophytic bacteria were isolated from pepper stem and crown and forty-nine bacteria from soil. Isolates were obtained from pepper grown fields of Bozova and Hilvan Districts of Şanlıurfa. Antagonistic effects of endophytic bacteria and soil-borne bacteria were in-vitro tested against Fusarium solani, F. oxysporum, Macrophomina phaseolina and Rhizoctonia solani in petri dishes. Bacterial isolates from soil and endophytic bacteria revealed antagonistic activity of 79.37-20.22% and 81.69-33.47%, respectively against four pathogens.Statistically significant differences were found among the efficiencies against four different pathogens. Additionally, bacteria isolated from Bozova province were found to have higher antagonistic activity than those of Hilvan province. The antagonistic activity of endophytic bacteria was found to be higher than that of bacterial isolates from soil. In later studies, effective endophytic bacteria isolated from pepper can be used against soilborne pathogensConference Object Bipolaris sorokiniana associated with common root rot in Azerbaijan and Kazakhstan(2022) Derviş, Sibel; Bozoğlu, Tuğba; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, GökselWheat (Triticum spp.) is the third most important crop in terms of global production, with an average annual production of almost 219 million ha and yielding 760.9 million tons, after maize and rice. However, A complex of fungi attacking the crown and root tissues of wheat causes a serious problem and significant yield reductions in wheat. Although most prevailing species in this complex can change yearly and regionally in dryland winter wheat production areas, Bipolaris sorokiniana Shoemaker (teleomorph: Cochliobolus sativus) is one of the dominant species and causes spot blotch and common root rot (CRR) on wheat plants, especially in winter wheat varieties. Comprehensive surveys were conducted to identify B. sorokiniana associated with CRR of wheat throughout the main wheat-growing areas of Azerbaijan and Kazakhstan in 2017 and 2019, respectively. As a result of the classification of species based on morphological and molecular tools, 96 and 547 isolates were identified as B. sorokiniana for Azerbaijan and Kazakhstan, respectively. All surveyed regions for these countries were contaminated with this pathogen. Some representative isolates of B. sorokiniana were tested for their ability to cause disease and produced moderate disease severities on cultivar Seri 82 (Triticum aestivum, bread wheat). The percent incidence of B. sorokiniana isolates in all isolates was 15.2% for Azerbaijan, whereas, for Kazakhstan, B. sorokiniana was the most frequently recovered species in the three regions surveyed, with an isolated frequency of 44.80%. The results of the current study provide crucial and helpful information to improve disease management strategies against CRR of wheat in these countries.Article Bitki Gelişimini Teşvik Eden Bazı Mikroorganizmalar(2009) Güler Güney, İnci; Güler Güney, İnciBitki gelişimini arttıran Rhizobium spp. , Azospirillum spp. ve Glomus spp. gibi mikroorganizmalar hastalık kontrolünde de etkili olmaktadır. Pseudomonas spp. ve Trichoderma spp. gibi biyolojik mücadele etmenlerinin ise bitki gelişimini teşvik ettikleri son yıllarda yapılan çalışmalarda ortaya konmuştur. Tarımsal ürünleri hastalıklara karşı korumak ve gelişimlerini arttırmak için, bu mikroorganizmaların uygun zamanda ve miktarlarda kullanımları sağlanmalıdır. Böylece kimyasal gübrelerin kullanımları sonucu oluşan problemler azaltılabilir. Bu derlemede, biyolojik gübre etmeni olarak kullanılan mikroorganizmaların hem bitki gelişimini teşvik etmeleri hem de biyolojik mücadelede kullanılmaları ile ilgili yapılan çalışmalar özetlenmiştir.Article Correction to: Leaf spot caused by Alternaria crassa on Datura stramonium in Turkey(Australasian Plant Disease Notes, 2022) Derviş, Sibel; Alkan, Mehtap; Derviş, Sibel; Özer, GökselIn August 2021, jimson weed (Datura stramonium) plants growing as weeds in potato fields in Bolu province, Turkey, exhibited leaf spots with dark concentric rings. Sunken and lens-shaped lesions with a light center were also frequently observed on petioles, branches, and stems. Based on morphological characteristics and phylogenetic analysis of the transcription elongation factor 1-α, RNA polymerase second largest subunit, and glyceraldehyde-3- phosphate dehydrogenase loci, the causal agent was identified as Alternaria crassa. The pathogen was successfully re-isolated from inoculated jimson weed plants in the pathogenicity assay, proving Koch’s postulates. Alternaria crassa caused necrotic lesions on potato plants, similar to those of early blight, confirming them as an alternative host of the pathogen. This is apparently the first report of leaf spot caused by A. crassa on jimson weed in Turkey.Conference Object Evaluation of inhibitory effect some bicarbonate carbonate salts against Neoscytalidium dimiatum(2023) Derviş, Sibel; Türkkan, Muharrem; Özer, Göksel; Derviş, Sibel; Erper, İsmailNeoscytalidium dimidiatum, a member of the Botryosphaeriaceae family, has emerged as a significant pathogen causing canker and blight diseases in various fruit trees, field crops, park and forest trees, and other hosts. Neoscytalidium dimidiatum is the only recognized species within the genus, with the other two previously recognized species, N. novaehollandiae and N. orchidacearum. There is no suggested chemical control method against Neoscytalidium. In this study, we evaluated the inhibitory effect of some salts on the mycelial growth, germ tube elongation and conidial germination of the N. dimidiatum isolate phylogenetically clustered with the isolates previously recognized as N. novaehollandiae. The antifungal effects of the salts were determined at 0.125, 0.25, 0.5, 1.0 and 2.0% (w/v) concentrations of salts in vitro conditions. Statistically, differences were observed between the inhibitory effects of six salts on the parameters at P ≤ 0.05. The 0.25% and higher concentrations of the ammonium bicarbonate and ammonium carbonate salts completely inhibited mycelial growth, germ tube elongation and conidial germination. Potassium and sodium bicarbonate salts in the highest concentration (2.0%) were able to decrease the mycelial growth, germ tube elongation and conidial germination with the rates of 95.58%-95.58%, 95.05%-95.76% and 77.44%-82.91%, respectively. In addition, potassium and sodium carbonate were able to completely inhibit investigated factors of the pathogen at 2.0% and 1.0% concentrations, respectively. The minimum inhibition concentration values of the ammonium bicarbonate, potassium carbonate and sodium carbonate varied between 0.25% and 2.0%. The minimum fungicidal concentration values of the ammonium carbonate and bicarbonate also ranged between 0.25% and 1.0%. As a result, this study showed that salts of carbonate and bicarbonate could be recommended to manage diseases caused by N. dimidiatum.Article Factors influencing adaptation of innovations in small ruminant production in the TRC3 Region in Turkey(Emirates Journal of Food and Agriculture, 2021) Acıbuca, VeysiThis study investigated the socio-economic factors affecting the adoption of innovation in small ruminant production in TRC3 region in Turkey. Stratified random sampling technique was used to select 366 small ruminant farmers from the region. Semi-structured questionnaire was used to collect main data for the study. Data collected were analyzed using descritive statistics, t-tests and multiple linear regression analysis. Results of the study showed that the farmers had a mean age of 45,8 years and 97,0% were male. Traditional production was dominant among the small ruminant producers and as the number of animal increases the income increases (p< 0,01). Out of twelve independent variables, eight of them were statistically significant on multiple linear regression analysis. The F-statistics was statistically significant at 1% level on communication behavior and animal health practices; 5% level on age, breeding reasons, milking way, produced products and fold type. The coefficient of multiple determination (R2) value was estimated to be 0,470 this implies that 47,0% of total variation in the output of small ruminant production was accounted for by the independent variables that were fitted into the model. The main problems of small ruminant production were high feed prices, high deaths of lamps/goat, insufficient use of pastures because of security problems and lack of shepherd due to young people unwillingness to live in rural areas.Conference Object Fungi isolated from cankered tissues of declining apricot trees in Malatya and Elazığ provinces of Turkey(2017) Derviş, Sibel; Çiftçi, Osman; Derviş, SibelSurveys were carried out in apricot (Prunus armeniaca L.) production areas of Malatya and Elazığ provinces from April to November in 2015 and 2016. Fungal and oomycetous diseases causing dieback and decline symptoms were investigated and locations where the diseases were prevalent were determined according to the districts in these provinces. Nine and 40 orchards were visited in Elazığ and Malatya during the course of the surveys. A total of 665 out of 5750 apricot trees were checked and the disease incidence was found to be 44% in the surveyed orchards. Out of isolates obtained from root and crown tissues of symptomatic trees, isolates obtained from cankered tissues were characterized according to their morphological characteristics. Genomic DNA was extracted from representative isolates. The internal transcribed spacer (ITS) region of rDNA was amplified using the ITS6/ITS4 primer pair and sequenced and submitted to GenBank. NCBI BLAST results showed 98 to 100% similarity with the ITS sequences of many Clonostachys rosea f. rosea (Link : Fr.) Schroers et. al. 1999 (Ascomycetes, Hypocreales), Sarocladium kiliense (Grütz) Summerb. 2011 (Ascomycetes, Incertae sedis) (Syn: Acremonium kiliense), Phoma sp. (Ascomycetes, Pleosporales), Entoleuca spp. (Ascomycetes, Xylariales) strains deposited in NCBI GenBank. The sequences were submitted to GenBank and given accession numbers were MF536537 and MF536538 for C. rosea, MF536539 for S. kiliense, MF536540 and MF536541 for Phoma spp., and MF536542, MF536543, MF536544 and MF536545 for Entoleuca spp. isolates. Moreover, Verticillium dahliae and Macrophomina phaseolina were also isolated from inner tissues of necrotic branches and morphologically identified. However, pathogenicity of these isolates needs further investigations. If some isolates were not pathogenic, their endophytic or hperparasitic characteristics against pathogenic ones should be tested in order to fully exploit their potential for use as biological control agents.Conference Object Fusarium spp. associated with crown and root rot in Azerbaijan and Kazakhstan(2022) Derviş, Sibel; Derviş, Sibel; Paulitz,Timothy C.; Özdemir, Fatih; Morgounov, Alexey; Mumınjanov,Hafiz; Amer, Mohammed; Imren, Mustafa; Dababat, Abdelfattah A.; Özer, GökselLittle is known about the distribution and prevalence of pathogens of underground parts of winter wheat in Azerbaijan and Kazakhstan. In this study, a detailed survey study was conducted to assess the distribution of pathogen the country to generate information and understand disease dynamics, as well as to test the pathogenicity of the obtained species on a susceptible wheat cultivar.Wheat (Triticum spp.) is a primary source of calories and protein (Shiferaw et al. 2013), grown on 219 million ha and yielding 760.9 million tons (FAOSTAT 2022). Bread wheat (Triticum aestivum L.) and durum wheat (T. durum Desf.) provide a major contribution to the diets of humans and livestock in Azerbaijan and Kazakhstan, with an average annual production of about 1.82 million tons in a planted area of about 0.59 million ha and with 14.3 million tons produced on 12.1 million ha, respectively. Especially, Kazakhstan’s wheat yield (1182.5 ton/ha) falls far short of the global average (3474.4 ton/ha) due to biotic and abiotic stressors.The complex fungal species attacking the crown and root tissues of wheat causes a serious problem, including damping-off, blight, necrosis, and dry rotting of the root, crown, sub-crown, and lower stem tissues, along with wilting and stunting of seedlings and mature wheat plants (Bockus et al. 2010), resulting in significant yield reductions in the major wheat-producing regions of the world (Gonzalez and Trevathan 2000).Template DNA was extracted from 50–100 mg of fungal powder using a DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. The translation elongation factor 1- alpha (EF1-α) gene and the internal transcribed spacer (ITS) region of ribosomal DNA were amplified with EF1/EF2 (O’Donnell et al. 1998) and ITS1/ITS4 (White et al. 1990) primer sets, respectively.Article Herbalists and herbs in Southeastern Anatolia of Turkey(Emirates Journal of Food and Agriculture., 2021) Acıbuca, VeysiIn this study, interview technique (face to face) was used to collect data from 56 herbalists in three provinces of the Southeastern Anatolia Region of Turkey (Mardin, Diyarbakir and Sanliurfa). The main purpose of the study was to determine the education level, learning methods of their job, professional experiences, the parts of the plants used and the problems of the herbalists. The average age of the herbalists were 41,7 and majority of them had a high school diploma. They learned this job mainly from older family members or working at the herbal shop. They mainly obtained their products from local people and wholesalers. Especially spices, food supplements, treatments, cosmetic and weight loss products were sold by herbalists.Article Inoculation Techniquesfor Assessing PathogenicityofRhizoctonia solani, Macrophomina phaseolina,Fusarium oxysporumand Fusarium solani on Pepper Seedlings(2018) Güler Güney, İnci; Güldür, Mehmet ErtuğrulIn this study, surveys were carried out during 2015 and 2016 for wilt and root rot diseases caused by Rhizoctonia solani, Macrophomina phaseolina, Fusarium oxysporum, and Fusarium solani in pepper fields in Adıyaman, Diyarbakır, Mardin and Şanlıurfa provinces of Turkey. The purpose of this study was to evaluate the effects of differentinoculation methods (root dip, soil infestation with wheat bran and soil infestation with rice grain) on pathogenicities of R. solani, M. phaseolina,F. oxysporum andF. solani on pepper seedlings. Inoculated pepper seedlings (cv. İnan-3363) were left to grow for three months after transplanting under growth chamber conditions. Inoculation of infective rice-grain was used to test pathogenicity of all four fungi. Root dip inoculation method was used for F. solani and F. oxysporum when the soil was infestedwith wheat branmethod for R. solani andM. phaseolina inoculation. Alltested isolates resultedin thestemand root rot, leaf chlorosis and bruising. To test the pathogenicity of fungi, soil infestation with rice grain inoculation was the most suitable method.All tested fungiinduced similarfoliar symptoms,root rot severityand caused asimilarreduction in dryroot weightswhen rice-grain inoculum wasused. With other inoculation methods, all pathogens similarly affected root rot severity. Whereas, F. oxysporum was the least virulent pathogen among tested fungi affecting foliar symptom severity for fresh root and plant weights, and dry root and plant weights R. solani, M. phaseolina andF. solani weresimilarlyvirulent when these parameters were used. The results of the present study may have a useful connotation to monitor pepper seedlings against these pathogens. In conclusion, we recommend rice-grain inoculation to test pathogenicities of R. solani, M. phaseolina, F. oxysporumandF. solanion various pepper cultivars.Book Part İNSANSIZ HAVA ARAÇLARININ TARIMSAL FAALİYETLERDE KULLANIMI VE GELECEĞE YÖNELİK BEKLENTİLER(İKSAD YAYINEVİ, 2023)İNSANSIZ HAVA ARAÇLARIArticle Interregional comparative analysis of farmers’ perceptions and expectations of climate change(Italian Journal of Agronomy, 2022) Acıbuca, VeysiThis study looked into the relationship and effects of agricultural activities in different regions of Turkey on climate change. This study aims to determine farmer awareness of climate change and its effects, as well as farmer adaptation capabilities in different regions of Turkey against climate change, and to develop extension and policy tools based on the findings. Data were collected through face-to-face surveys with farmers in the provinces where the research was conducted. In this context, a proportional sampling survey of 418 farmers was conducted. The collected information was subjected to factor analysis and the independent t-test. According to the findings, farmers associate climate change with precipitation, and the effect of precipitation and the risks it poses in increasing or decreasing crop yields are of particular concern. Farmers in the Southeastern Anatolia Region are more concerned about heat and drought than farmers in the Mediterranean Region. Furthermore, producers believe that human-caused factors and economic development have a greater impact on climate change than agricultural activities. Farmers in research areas are concerned that climate change will increase migration from rural areas and the decline of forests and animal species. As a result, raising individual awareness and utilising new technology in rural areas is critical. Farmers’ awareness of new and environmentally friendly agricultural techniques must be raised to increase their use.Article ITS and LSU-rDNA nucleotide sequences based confirmation of Cytospora chrysosperma and Chondrostereum purpureum from symptomatic cankered tissues of Populus nigra trees in Turkey(2017) Derviş, Sibel; Çiftçi, Osman; Türkölmez, Şahimerdan; Ulubaş Serçe, ÇiğdemMalatya ili Doğanşehir ilçesinde 2016 yılında yapılan arazi çalışmaları sırasında gövde, dal kanseri ve kuruma belirtileri gösteren kavak (Populus nigra) ağaçlarından alınan örneklerden yapılan laboratuar çalışmaları sonucunda piknidyum içeren kabukların altından ve odun dokularından sırasıyla Cytospora chrysosperma ve Chondrostereum purpureum izole edilmiştir. İlkbaharda, kavak ağaçlarının sürgünlerine, tamamen gelişmiş olan dördüncü yapraklarının koparılması sonucu ortaya çıkan yaralar üzerine, C. chrysosperma ve C. purpureum izolatları tarafından kolonize edilmiş agar disklerinin yerleştirilmesiyle inokulasyon yapılmıştır. İnokülasyondan üç ay sonra C. chrysosperma ve C. purpureum ile inokulasyon bölgesinde sırasıyla 6,4 ve 3,3 cm uzunluğunda kanserler oluşmuş ve sürgünler büzüşmüştür. Benzer bir şekilde, serada gerçekleştirilen patojenite testlerinde, kabuk dokusunda oluşturulan yaraların bu izolatlar ile inokülasyonundan yaklaşık 14 gün sonra kanser oluşumu gerçekleşmiştir. Hastalanan bitkilerin dokularından yapılan izolasyonlarda C. chrysosperma ve C. purpureum’un tekrar izole edilmesi ile hastalık etmenlerinin bu funguslar olduğu doğrulanmıştır. Steril ortam diskleri ile inokule edilen kontrol sürgünlerdeki yaralarda kanser oluşmamıştır. Her fungal türün temsili izolatından tüm DNA’nın izolasyonu yapılmıştır. İzole edilen toplam DNA’lar, rDNA'nın internal transcribed spacer (ITS) ve large subunit (LSU) gen bölgeleri için sırasıyla ITS6/ITS4 ve NL1/NL4 primer çiftleri kullanılarak amplifiye edilmiş ve dizilenmiştir. BLAST analizleri sonucunda, daha önce Gen Bankası’nda kaydedilen birçok C. chrysosperma ve C. purpureum ITS ve LSU nükleotid dizisi ile %99 benzerlik göstermiştir. Bu diziler Gen Bankasına kaydedilmiştir. C. chrysosperma ve C. purpureum’nın ITS-rDNA için NCBI’dan verilen erişim numaraları sırasıyla MF536529 ve MF536531; LSU-rDNA için veriler erişim numaraları ise sırasıyla MF536530 ve MF536532’dir. Bu fungus etmenlerinin Türkiye'deki varlığı daha önce bildirilmekle birlikte bu çalışma, C. chrysosperma ve C. purpureum'un ITS ve LSU-rDNA nükleotid dizilerine dayanarak moleküler karakterizasyonlarının ilk raporudur.Conference Object ITS and LSU-rDNA nucleotide sequences based confirmation of Cytospora chrysosperma and Chondrostereum purpureum from symptomatic cankered tissues of Populus sp. trees in Turkey(2017) Derviş, Sibel; Çiftçi, Osman; Türkölmez, Şahimerdan; Ulubaş Serçe, ÇiğdemThe fungi Cytospora chrysosperma and Chondrostereum purpureum were isolated from orange-brown inner bark with pycnidia in the bark surface and underlying wood tissues of infected poplar plants (Populus sp.) with symptoms of stem and branch canker in Doğanşehir, Malatya, in 2016, respectively. Twigs of poplar trees were inoculated during their first season of growth by removing the fourth fully expanded leaves and placing agar plugs colonized by representative isolates of C. chrysosperma and C. purpureum over the resulting wounds. Three months after inoculation, cankers in 6.4 and 3.3 cm length formed by C. chrysosperma and C. purpureum, respectively, and twigs were girdled. Pathogenicity tests in a greenhouse experiment by shallow wounds made into the bark tissue and inoculation with these isolates in a similar manner also resulted in canker formation in and around inoculated wounds 14 days after inoculation. Subsequent re-isolations of C. chrysosperma and C. purpureum confirmed that these fungi were the causal agents of the disease, and no cankers formed in wounds that received only sterile plugs. DNA was extracted from representative isolates of each fungal species. Extracted DNA templates were amplified and sequenced for rDNA internal transcribed spacer (ITS) and the large subunit (LSU) rDNA gene regions using ITS6/ITS4 and NL1/NL4 primer pairs, respectively. NCBI BLAST results showed 99% similarity with the ITS and LSU sequences of C. chrysosperma and C. purpureum in GenBank. The sequences were submitted to GenBank. Given accession numbers of C. chrysosperma and C. purpureum were MF536529 and MF536531 for ITSrDNA; MF536530 and MF536532 for LSU-rDNA, respectively. Existence of these fungi in Turkey was previously reported. However, this is a first report of molecular characterization of C. chrysosperma and C. purpureum based on ITS and LSU-rDNA nucleotide sequences of these fungi in Turkey.Article Land Suitability Assessment for Pistachio Cultivation Using GIS and Multi‑Criteria Decision‑Making: A Case Study of Mardin, Turkey(Environ Monit Assess, 2023)Site selection for pistachio orchards is an important issue for sustainable agricultural policies, crop productivity, agricultural planning, and communities. This study aims to investigate suitable places for pistachio in the Mardin Province (SE Turkey) by considering several variables, such as meteorological data, topographic conditions, economic factors, and soil characteristics, using Geographic Information System (GIS) and Multi-Criteria Decision Analysis. Pistachio farmers, expert opinions, and literature data were used to determine the requirements for pistachio cultivation. Four main assessment criteria (thirteen sub-criteria), sixty value ranges, and fourteen exclusion criteria were determined for the pistachio land suitability assessment. The weighting of the evaluation criteria was calculated using the Analytical Hierarchy Process (AHP). Farmers and experts have stated that meteorological factors are more important than soil, topography, and economic factors. All data were transferred to the GIS environment, and a land suitability map was created using the weighted linear combination method. The results show that Mardin province has very suitable lands for pistachio cultivation. The resulting map determined that the 228,891.59 ha area in Mardin province is very suitable for pistachio. To evaluate the accuracy of the land suitability map generated for pistachio, the Receiver Operating Characteristic (ROC) curve was used. The value of the area under the curve (AUC) was calculated to be 0.806, which indicates that the study is consistent. The created suitability map will be an essential data source for developing sustainable agricultural strategies in the Southeastern Anatolia region.Conference Object Mardin İlinde Anız Yakılan ve Yakılmayan Topraklarda Rizosfer Mikroorganizmalarının Belirlenmesi: Mısırda Anız Yakımı Topraktaki Bakteri ve FungusPopulasyonunu Etkiler mi?(2016) Güler Güney, İnci; Derviş, Sibel; Güldür, Mehmet ErtuğrulBu çalıĢma, mısır bitkilerinde anız yakımının, rizosfer toprağındaki (0-10 cm) bakteriyel ve fungal popülasyonlara etkisini gözlemlemek amacıyla kurgulanmıĢtır. Bu amaçla, anız yakımının yaygın bir Ģekilde uygulandığı Mardin ilinde 20 lokasyondan anız yakılmıĢ ve yakılmamıĢ mısır tarlalarının rizosfer tabakasının 0-2.5, 2.5- 5 ve 5-10 cm toprak derinliklerinden, toplam 120 örnek alınmıĢtır. Toprak örnekleri 10-1‟den 10-6‟ya kadar seyreltilerek bakteriyel ve fungal izolasyonlar yapılmıĢtır. Her toprak derinliği ve her seyreltme derecesi için 10 ar adet patates dekstroz agar (PDA) ve nutrient agar (NA) kullanılmıĢtır. Bakteriyel ve fungal izolasyonlar için kullanılan besi yerleri sırasıyla 2-4 gün ve 5-7 gün 28 °C‟de inkübe edilmiĢtir. Anız yakılmıĢ topraklarda 10-3 ve 10-4 seyreltmelerde 0-2.5, 2.5-5 ve 5-10cm toprak derinliklerinde sırasıyla 2,4x105, 2.8x105 ve 2,9 x105 kob (koloni oluĢturan birim) bakteri /g toprak; anız yakılmamıĢ topraklarda ise aynı konsantrasyonda ve aynı toprak derinliklerinde sırasıyla 3,3x105, 4 x105 ve 3,8x105 kob bakteri /g toprak sayımı yapılmıĢtır. Anız yakılmıĢ topraklarda 10-3 ve 10-4 konsantrasyonda 0-2.5, 2.5-5 ve 5-10cm toprak derinliklerinde sırasıyla 1.2 x104, 3 x104 ve 1.3 x104 kob fungus/g toprak; anız yakılmamıĢ topraklarda ise aynı konsantrasyonda ve aynı toprak derinliklerinde sırasıyla 2.9 x104, 2.1 x105 ve 3.6 x104 kob fungus /g toprak sayımı yapılmıĢtır. Sonuç olarak, anız yakılmamıĢ topraklarda fungal ve bakteriyel koloni sayısının farklı toprak derinliklerinde anız yakılmıĢlara oranla daha fazla olduğu tespit edilmiĢtir. Anız yakılan topraklarda biyolojik aktivite düĢtüğü için, toprağın sürdürülebilirliğini ve verimliliğini artırmada daha uygun anız yönetim sistemlerinin uygulanması gerekliliğini öneriyoruz.Article Mardin İlinde Makarnalık Buğday Üretimi ve Üreticilerin Sorunları(MAS Journal of Applied Sciences, 2021) Acıbuca, VeysiBu çalışmada 2020 yılı verilerine göre Türkiye’de makarnalık buğday üretiminin % 8,3’ünün üretildiği Mardin ilinde makarnalık buğday üretimi yapan çiftçilerin sosyo-ekonomik durumu, makarnalık buğday üretimi yapmalarına etki eden faktörler, ürünün pazarlama yapısı ve üreticilerin sorunları araştırılmış olup bu amaçla 3 ilçede (Artuklu, Kızıltepe ve Derik) tabakalı tesadüfi örnekleme yöntemiyle hesaplanmış 120 üreticiyle yüz yüze anket çalışması yapılmıştır. İlde makarnalık buğday üretimine etki eden en önemli faktörlerin buğdayın fiyatı ve pazarlanma durumu olduğu, yörede sertifikalı ve yüksek verimli tohum kullanımının oldukça yüksek olduğu ve işletmelerin % 61.7’sinde sulama yapıldığı bilgisi alınmıştır. Üreticilerin makarnalık buğday üretiminde karşılaştıkları en önemli sorunların başında sulamada kullanılan enerji ücretinin çok yüksek olması gelmektedir. Girdi fiyatlarının yüksek olması ile kaliteli tohum temininde yaşanan sıkıntılar diğer sorunlar arasında bulunmaktadır. Sonuç olarak ilde makarnalık buğday üretimini kısıtlayan faktörlerin minimum seviyeye düşürülmesi ve bölgemizde makarnalık buğday ekilişinin giderek azaldığı göz önünde bulundurularak ilave/alternatif destekleme modelleri ile söz konusu ürünün ekim alanlarının arttırılması önerilmiştir.Book Part Mardin’de Tarım Sektörünün Temel Sorunları ve Potansiyel İş Birliği Alanları(MAÜ Yayınevi, 2023) Acıbuca, Veysia) Amaç: Bu bölümün amacı; Mardin ilinde tarım sektörünün temel sorunlarının tespit edilmesi ve Mardin Artuklu Üniversitesi ile iş birliği alanlarının ortaya çıkarılmasıdır. b) Bulgular: Mardin ilinde tarım üreticilerinin karşılaştığı temel sorunların başında enerji giderlerinin ve diğer girdi maliyetlerinin yüksek olması gelmektedir. Pazarlama, Ar-Ge, markalaşma ve kalifiye eleman yetiştirilmesi gibi alanlardaki sorunlar ise ön plana çıkmaktadır. c) Teklifler: Katılımcılar, ilde tarım sektörünün sorunlarının Mardin Artuklu Üniversitesi ile yürütülecek iş birlikleri ile çözülebileceğini belirtmektedirler. Bu çerçevede Üniversiteden; sorunların gündemde tutulması, çiftçilerin ve üreticilerin bilinçlendirilmesi, kalifiye eleman temin edilmesi, ihracat desteklerine dair bilgi sağlanması ve alternatif pazar arayışları gibi konularda iş birliği talep etmektedirler.